In-vitro blood vessel regeneration

ABSTRACTBasic fibroblast growth factor (bFGF), a protein, plays a key role in wound healing and blood vessel regeneration. However, most negative effects in vivo, or in vitro result from the over dosage of bFGF. Furthermore, it needs to keep the bFGF from protein denaturant. Thus, this study aims to develop a new delivery system based on silica nanoparticles (SiO2 NPs) dispersed in collagen patch for delivery of the bFGF in a local and prolonged manner. In this research, SiO2 NPs are used to encapsulate bFGF through a modified water-in-oil micro-emulsion. The bFGF-loaded nanoparticles afterwards are dispersed in the collagen-based matrix through a EDC cross-linking step. The in vitro release kinetics of SiO2 NPs - encapsulated bFGF with and without collagen matrix have been monitored through ELISA. In addition, the cytotoxicity of SiO2 NPs is investigated by studying the viability of Human Umbilical Vein Endothelial Cells (HUVEC) under the different concentrations of SiO2 NPs. It has found the average diameter (d) for SiO2 NPs encapsulating bFGF is 45 ± 8 nm with a loading efficiency of 72.5±3%. The maximum concentration of bFGF locally released from SiO2 NPs impregnated collagen matrix can be monitored after 30 days, while bFGF released from SiO2 NPs can be detected in 20 days. The further prolonged releasing after the nanoparticle-encapsulated bFGF laden collagen matrix is possibly due to the interaction between the nanoparticles and collagen matrix. In addition, the biocompatibility of the SiO2 NP has been investigated. We found that SiO2 NPs at the concentration of 50 μg/ml can still keep the cell alive. The results indicate that the nanoparticle-laden collagen matrix can locally deliver growth factor in a prolonged manner. This new delivery system may benefit to blood vessel regeneration and potentiate greater angiogenesis.

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Nanofiber-mediated microRNA-126 delivery to vascular endothelial cells for blood vessel regeneration

Acta Biomaterialia ◽

10.1016/j.actbio.2016.07.048 ◽

2016 ◽

Vol 43 ◽

pp. 303-313 ◽

Cited By ~ 51

Author(s):

Fang Zhou ◽

Xiaoling Jia ◽

Yang Yang ◽

Qingmao Yang ◽

Chao Gao ◽

...

Keyword(s):

Endothelial Cells ◽

Blood Vessel ◽

Vascular Endothelial Cells ◽

Vascular Endothelial ◽

Vessel Regeneration

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Bio-Medico-Mechanical Behavior of Natural Artery Blood Vessel Under Constant and Variable Internal Pulsatile Pressure Flow Test In Vitro

Journal of Biomechanical Engineering ◽

10.1115/1.3138617 ◽

1986 ◽

Vol 108 (4) ◽

pp. 295-300 ◽

Cited By ~ 4

Author(s):

A. T. Yokobori ◽

T. Maeyama ◽

T. Ohkuma ◽

T. Yokobori ◽

H. Ohuchi ◽

...

Keyword(s):

Blood Pressure ◽

Blood Vessel ◽

Mechanical Behavior ◽

Arterial System ◽

Pressure Amplitude ◽

Constant Amplitude ◽

Pressure Flow ◽

Fluctuating Pressure ◽

Pulsatile Pressure

Studies have been carried out on the bio-medico-mechanical behavior in vitro of natural blood vessel (dogs) under constant and variable internal pulsatile pressure flow. The apparatus designed by us well simulated the arterial system. The studies were made for the case of pressure amplitude kept as constant, of the two-step-multi-duplicated pulsatile pressure and of the fluctuating pressure. For the case of the fluctuating pressure, the strength of the artery becomes considerably lower than those under constant amplitude and two-step-multi-duplicated pulsatile pressure. SEM observations of the inner walls of the artery shows that collagen fibers are more elongated under fluctuating pulsatile pressure flow. In conclusion, in order to avoid the mechanical deterioration of the artery strength, it is useful to keep the pulsatile blood pressure at constant amplitude. Even for the case of the blood pressure fluctuation, it is necessary to manage to keep the blood pressure as near a regular wave as possible, the total number of repeated pulse being equal.

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In Vitro Blood Compatibility of Novel Hydrophilic Chitosan Films for Vessel Regeneration and Repair

Advances in Biomaterials Science and Biomedical Applications ◽

10.5772/52706 ◽

2013 ◽

Cited By ~ 2

Author(s):

Antonello A. ◽

Luigi Ippolito ◽

Federica Riccardi ◽

Silvia Pipitone ◽

Marina Morganti ◽

...

Keyword(s):

Blood Compatibility ◽

Chitosan Films ◽

Vessel Regeneration

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Sustained VEGF delivery via PLGA nanoparticles promotes vascular growth

AJP Heart and Circulatory Physiology ◽

10.1152/ajpheart.00199.2009 ◽

2010 ◽

Vol 298 (6) ◽

pp. H1959-H1965 ◽

Cited By ~ 92

Author(s):

Justin S. Golub ◽

Young-tae Kim ◽

Craig L. Duvall ◽

Ravi V. Bellamkonda ◽

Divya Gupta ◽

...

Keyword(s):

Tissue Engineering ◽

Blood Vessel ◽

Femoral Artery ◽

Release Kinetics ◽

Aortic Ring ◽

Plga Nanoparticles ◽

Cardiovascular Medicine ◽

Vessel Growth ◽

Vessel Volume

Technologies to increase tissue vascularity are critically important to the fields of tissue engineering and cardiovascular medicine. Currently, limited technologies exist to encourage angiogenesis and arteriogenesis in a controlled manner. In the present study, we describe an injectable controlled release system consisting of VEGF encapsulated in poly(lactic- co-glycolic acid) (PLGA) nanoparticles (NPs). The majority of VEGF was released gradually over 2–4 days from the NPs as determined by an ELISA release kinetics experiment. An in vitro aortic ring bioassay was used to verify the bioactivity of VEGF-NPs compared with empty NPs and no treatment. A mouse femoral artery ischemia model was then used to measure revascularization in VEGF-NP-treated limbs compared with limbs treated with naked VEGF and saline. 129/Sv mice were anesthetized with isoflurane, and a region of the common femoral artery and vein was ligated and excised. Mice were then injected with VEGF-NPs, naked VEGF, or saline. After 4 days, three-dimensional microcomputed tomography angiography was used to quantify vessel growth and morphology. Mice that received VEGF-NP treatment showed a significant increase in total vessel volume and vessel connectivity compared with 5 μg VEGF, 2.5 μg VEGF, and saline treatment (all P < 0.001). When the yield of the fabrication process was taken into account, VEGF-NPs were over an order of magnitude more potent than naked VEGF in increasing blood vessel volume. Differences between the VEGF-NP group and all other groups were even greater when only small-sized vessels under 300 μm diameter were analyzed. In conclusion, sustained VEGF delivery via PLGA NPs shows promise for encouraging blood vessel growth in tissue engineering and cardiovascular medicine applications.

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Ingrowth of aorta vascular cells into basic fibroblast growth factor-impregnated vascular prosthesis material: A porcine and human in vitro study on blood vessel prosthesis healing

Journal of Vascular Surgery ◽

10.1067/mva.2002.128932 ◽

2002 ◽

Vol 36 (6) ◽

pp. 1237-1247 ◽

Cited By ~ 12

Author(s):

J.M.Annemieke van der Bas ◽

Paul H.A. Quax ◽

Arjen C. van den Berg ◽

Victor W.M. van Hinsbergh ◽

J.Hajo van Bockel

Keyword(s):

Growth Factor ◽

Blood Vessel ◽

Fibroblast Growth Factor ◽

Basic Fibroblast Growth Factor ◽

In Vitro Study ◽

Vascular Prosthesis ◽

Fibroblast Growth ◽

Blood Vessel Prosthesis ◽

Prosthesis Material

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Fabrication of Hollow Structures in Photodegradable Hydrogels Using a Multi-Photon Excitation Process for Blood Vessel Tissue Engineering

Micromachines ◽

10.3390/mi11070679 ◽

2020 ◽

Vol 11 (7) ◽

pp. 679

Author(s):

Uran Watanabe ◽

Shinji Sugiura ◽

Masayuki Kakehata ◽

Fumiki Yanagawa ◽

Toshiyuki Takagi ◽

...

Keyword(s):

Tissue Engineering ◽

Blood Vessel ◽

Microfluidic Device ◽

Vascular Function ◽

Laser Scanning ◽

Crosslinking Reaction ◽

Excitation Process ◽

Hollow Structures ◽

Photon Excitation

Engineered blood vessels generally recapitulate vascular function in vitro and can be utilized in drug discovery as a novel microphysiological system. Recently, various methods to fabricate vascular models in hydrogels have been reported to study the blood vessel functions in vitro; however, in general, it is difficult to fabricate hollow structures with a designed size and structure with a tens of micrometers scale for blood vessel tissue engineering. This study reports a method to fabricate the hollow structures in photodegradable hydrogels prepared in a microfluidic device. An infrared femtosecond pulsed laser, employed to induce photodegradation via multi-photon excitation, was scanned in the hydrogel in a program-controlled manner for fabricating the designed hollow structures. The photodegradable hydrogel was prepared by a crosslinking reaction between an azide-modified gelatin solution and a dibenzocyclooctyl-terminated photocleavable tetra-arm polyethylene glycol crosslinker solution. After assessing the composition of the photodegradable hydrogel in terms of swelling and cell adhesion, the hydrogel prepared in the microfluidic device was processed by laser scanning to fabricate linear and branched hollow structures present in it. We introduced a microsphere suspension into the fabricated structure in photodegradable hydrogels, and confirmed the fabrication of perfusable hollow structures of designed patterns via the multi-photon excitation process.

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Vascular Tissue Engineering: Recent Advances in Small Diameter Blood Vessel Regeneration

ISRN Vascular Medicine ◽

10.1155/2014/923030 ◽

2014 ◽

Vol 2014 ◽

pp. 1-27 ◽

Cited By ~ 59

Author(s):

Valentina Catto ◽

Silvia Farè ◽

Giuliano Freddi ◽

Maria Cristina Tanzi

Keyword(s):

Tissue Engineering ◽

Cardiovascular Diseases ◽

Blood Vessel ◽

Polyethylene Terephthalate ◽

Vascular Tissue ◽

Vascular Grafts ◽

Small Diameter ◽

Synthetic Polymers ◽

Vascular Tissue Engineering ◽

Vessel Regeneration

Cardiovascular diseases are the leading cause of mortality around the globe. The development of a functional and appropriate substitute for small diameter blood vessel replacement is still a challenge to overcome the main drawbacks of autografts and the inadequate performances of synthetic prostheses made of polyethylene terephthalate (PET, Dacron) and expanded polytetrafluoroethylene (ePTFE, Goretex). Therefore, vascular tissue engineering has become a promising approach for small diameter blood vessel regeneration as demonstrated by the increasing interest dedicated to this field. This review is focused on the most relevant and recent studies concerning vascular tissue engineering for small diameter blood vessel applications. Specifically, the present work reviews research on the development of tissue-engineered vascular grafts made of decellularized matrices and natural and/or biodegradable synthetic polymers and their realization without scaffold.

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