scholarly journals Vascular Tissue Engineering: Recent Advances in Small Diameter Blood Vessel Regeneration

2014 ◽  
Vol 2014 ◽  
pp. 1-27 ◽  
Author(s):  
Valentina Catto ◽  
Silvia Farè ◽  
Giuliano Freddi ◽  
Maria Cristina Tanzi
Keyword(s):  
Tissue Engineering ◽  
Cardiovascular Diseases ◽  
Blood Vessel ◽  
Polyethylene Terephthalate ◽  
Vascular Tissue ◽  
Vascular Grafts ◽  
Small Diameter ◽  
Synthetic Polymers ◽  
Vascular Tissue Engineering ◽  
Vessel Regeneration

Cardiovascular diseases are the leading cause of mortality around the globe. The development of a functional and appropriate substitute for small diameter blood vessel replacement is still a challenge to overcome the main drawbacks of autografts and the inadequate performances of synthetic prostheses made of polyethylene terephthalate (PET, Dacron) and expanded polytetrafluoroethylene (ePTFE, Goretex). Therefore, vascular tissue engineering has become a promising approach for small diameter blood vessel regeneration as demonstrated by the increasing interest dedicated to this field. This review is focused on the most relevant and recent studies concerning vascular tissue engineering for small diameter blood vessel applications. Specifically, the present work reviews research on the development of tissue-engineered vascular grafts made of decellularized matrices and natural and/or biodegradable synthetic polymers and their realization without scaffold.

Fabrication of PCL/keratin composite scaffolds for vascular tissue engineering with catalytic generation of nitric oxide potential

2020 ◽  
Vol 8 (28) ◽  
pp. 6092-6099
Author(s):  
Pengfei Li ◽  
Yanfang Wang ◽  
Xingxing Jin ◽  
Jie Dou ◽  
Xiao Han ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Tissue Engineering ◽  
Blood Vessel ◽  
Vascular Tissue ◽  
Vascular Grafts ◽  
Small Diameter ◽  
Vascular Tissue Engineering ◽  
Composite Scaffolds ◽  
Promising Solution ◽  
Blood Vessel Substitutes

Tissue-engineered vascular grafts (TEVGs) have been proposed as a promising solution to fulfill the need for small-diameter blood vessel substitutes.

Engineering blood vessels and vascularized tissues: technology trends and potential clinical applications

2019 ◽  
Vol 133 (9) ◽  
pp. 1115-1135 ◽  
Author(s):  
Prafulla Chandra ◽  
Anthony Atala
Keyword(s):  
Tissue Engineering ◽  
Vascular Tissue ◽  
Vascular Grafts ◽  
Small Diameter ◽  
Clinical Applications ◽  
Angiogenic Factors ◽  
Vascular Tissue Engineering ◽  
Technology Trends

Abstract Vascular tissue engineering has the potential to make a significant impact on the treatment of a wide variety of medical conditions, including providing in vitro generated vascularized tissue and organ constructs for transplantation. Since the first report on the construction of a biological blood vessel, significant research and technological advances have led to the generation of clinically relevant large and small diameter tissue engineered vascular grafts (TEVGs). However, developing a biocompatible blood-contacting surface is still a major challenge. Researchers are using biomimicry to generate functional vascular grafts and vascular networks. A multi-disciplinary approach is being used that includes biomaterials, cells, pro-angiogenic factors and microfabrication technologies. Techniques to achieve spatiotemporal control of vascularization include use of topographical engineering and controlled-release of growth/pro-angiogenic factors. Use of decellularized natural scaffolds has gained popularity for engineering complex vascularized organs for potential clinical use. Pre-vascularization of constructs prior to implantation has also been shown to enhance its anastomosis after implantation. Host-implant anastomosis is a phenomenon that is still not fully understood. However, it will be a critical factor in determining the in vivo success of a TEVGs or bioengineered organ. Many clinical studies have been conducted using TEVGs, but vascularized tissue/organ constructs are still in the research & development stage. In addition to technical challenges, there are commercialization and regulatory challenges that need to be addressed. In this review we examine recent advances in the field of vascular tissue engineering, with a focus on technology trends, challenges and potential clinical applications.

Multilayer Hybrid Construct for Vascular Tissue Engineering

2011 ◽  
Author(s):  
Krishna Madhavan ◽  
Walter Bonani ◽  
Wei Tan
Keyword(s):  
Tissue Engineering ◽  
Vascular Tissue ◽  
Vascular Grafts ◽  
Small Diameter ◽  
Vascular Tissue Engineering ◽  
Graft Material ◽  
Cell Assays ◽  
Recent Developments ◽  
And Function

Vascular grafts are often used as blood vessel substitutes. Until now, synthetic materials have not matched the efficacy of native tissues, particularly in the applications of small-diameter vascular grafts (<6mm) such as bypass grafts for arthrosclerosis and vascular access graft for hemodialysis. There is a considerable need for alternatives to the autologous veins or arteries. Many patients do not have an autologous vessel suitable for use due to preexisting pathological conditions or previous surgical harvest. Recent developments in vascular tissue engineering demonstrate the possibility of a biodegradable graft material containing living cells to mimic the structure and function of native vessels. However, fabrication of biomimetic grafts is often time and labor intensive, and subsequently requires complicated storage. This demands technology advancements in producing vessel mimetic grafts, considering their availability in addition to efficacy. To this end, new approaches to constructing small-diameter grafts that are of immediate availability and capable of regenerating biomimetic blood vessels in vivo may address the unmet demand in this area. We have designed a novel multilayer vascular construct which is made up of a nanofibrous “intima-equivalent” with thrombus-resistant vessel lumen and a porous biopolymer matrix as “media-equivalent” to allow smooth muscle cells (SMC) from native artery to grow and remodel the tissue. In this study, various layering strategies have been explored. To evaluate the resultant multilayer construct, structural, biochemical and biomechanical characterizations, as well as cell assays and short-term animal studie have been performed.

Surface Modification by Nanobiomaterials for Vascular Tissue Engineering Applications

2020 ◽  
Vol 27 (10) ◽  
pp. 1634-1646 ◽  
Author(s):  
Huey-Shan Hung ◽  
Shan-hui Hsu
Keyword(s):  
Tissue Engineering ◽  
Vascular Tissue ◽  
Thrombus Formation ◽  
Vascular Grafts ◽  
Vascular Tissue Engineering ◽  
Great Success ◽  
Natural Polymers ◽  
Vascular Biomaterials

Treatment of cardiovascular disease has achieved great success using artificial implants, particularly synthetic-polymer made grafts. However, thrombus formation and restenosis are the current clinical problems need to be conquered. New biomaterials, modifying the surface of synthetic vascular grafts, have been created to improve long-term patency for the better hemocompatibility. The vascular biomaterials can be fabricated from synthetic or natural polymers for vascular tissue engineering. Stem cells can be seeded by different techniques into tissue-engineered vascular grafts in vitro and implanted in vivo to repair the vascular tissues. To overcome the thrombogenesis and promote the endothelialization effect, vascular biomaterials employing nanotopography are more bio-mimic to the native tissue made and have been engineered by various approaches such as prepared as a simple surface coating on the vascular biomaterials. It has now become an important and interesting field to find novel approaches to better endothelization of vascular biomaterials. In this article, we focus to review the techniques with better potential improving endothelization and summarize for vascular biomaterial application. This review article will enable the development of biomaterials with a high degree of originality, innovative research on novel techniques for surface fabrication for vascular biomaterials application.

scholarly journals Engineering the mechanical and biological properties of nanofibrous vascular grafts for in situ vascular tissue engineering

2017 ◽  
Vol 9 (3) ◽  
pp. 035007 ◽  
Author(s):  
Jeffrey J D Henry ◽  
Jian Yu ◽  
Aijun Wang ◽  
Randall Lee ◽  
Jun Fang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Vascular Tissue ◽  
Vascular Grafts ◽  
Biological Properties ◽  
Vascular Tissue Engineering

Elastin-Sprayed Tubular Scaffolds With Microstructures and Nanotextures for Vascular Tissue Engineering

2013 ◽  
Author(s):  
Jinah Jang ◽  
Junghyuk Ko ◽  
Dong-Woo Cho ◽  
Martin B. G. Jun ◽  
Deok-Ho Kim
Keyword(s):  
Tissue Engineering ◽  
Vascular Graft ◽  
Vascular Tissue ◽  
High Surface ◽  
Small Diameter ◽  
Vascular Tissue Engineering ◽  
Biophysical Properties ◽  
Fibrous Scaffold ◽  
Cardiovascular Tissue ◽  
Highly Porous

Development of a small-diameter vascular graft (<6 mm) have been challenging due to thrombosis and intimal hyperplasia [1]. To overcome this problem, cardiovascular tissue engineers have attempted to construct a highly porous and biocompatible fibrous scaffold providing a sufficient mechanical strength for the regeneration of a functional tissue [2–5]. Herein, we present a 3D tubular-shaped micro/nanofibrous composite-layered scaffold for vascular tissue engineering. The surface of scaffold has high surface roughness by introducing nanofibrous layer and the biophysical properties have been fulfilled by using microfibrous layer. Moreover, the atomized spraying technique is applied to spray elastin proteins, which is well known as an antithrombogenic material, on the surface of micro/nanofibrous composite-layered scaffold to introduce an appropriate antithrombogenic surface.

The Effect of Dynamical Strain on the Maturation of Collagen-Based Cell-Containing Scaffolds for Vascular Tissue Engineering

2011 ◽  
Vol 409 ◽  
pp. 152-157 ◽  
Author(s):  
Lucie Levesque ◽  
D. Mantovani
Keyword(s):  
Tissue Engineering ◽  
Smooth Muscle ◽  
Blood Vessel ◽  
Smooth Muscle Cells ◽  
Vascular Tissue ◽  
Muscle Cells ◽  
Collagen Fibrils ◽  
Vascular Tissue Engineering ◽  
Silicone Membrane ◽  
Custom Made

Diseases occurring to blood vessel are preferentially solved by replacing the vessel by an autologous graft. When it is not available, a synthetic graft is used which has low patency rates for small diameter (<6 mm) vessels. Tissue engineering of blood vessel aims to improve the performance of vascular substitutes. Bioreactors are used in vascular tissue engineering to mimic the mechanical and biochemical environment of blood vessel. A 2D bioreactor was custom made in order to impose a dynamical strain to silicone membrane receiving the collagen cell-based construct. Collagen gels with vascular smooth muscle cells cultured inside were subdued to maturation under dynamical uniaxial stretch regimes at 1Hz for 48 hours. The percentage of deformation encountered by the silicone membrane was measured by ImageJ. Collagen fibrils and porcine smooth muscle cells (PSMC) orientations were assessed by scanning electron microscopy (SEM). Results show that the study of mechanical conditioning on cell activity is an important issue for enhancing the alignment of collagen fibrils.

scholarly journals Applying elastic fibre biology in vascular tissue engineering

2007 ◽  
Vol 362 (1484) ◽  
pp. 1293-1312 ◽  
Author(s):  
Cay M Kielty ◽  
Simon Stephan ◽  
Michael J Sherratt ◽  
Matthew Williamson ◽  
C. Adrian Shuttleworth
Keyword(s):  
Tissue Engineering ◽  
Vascular Graft ◽  
Vascular Tissue ◽  
Small Diameter ◽  
Elastic Fibre ◽  
Vascular Tissue Engineering ◽  
Western Society ◽  
Elastic Recoil ◽  
Vessel Structure ◽  
Biomaterial Scaffolds

For the treatment of vascular disease, the major cause of death in Western society, there is an urgent need for tissue-engineered, biocompatible, small calibre artery substitutes that restore biological function. Vascular tissue engineering of such grafts involves the development of compliant synthetic or biomaterial scaffolds that incorporate vascular cells and extracellular matrix. Elastic fibres are major structural elements of arterial walls that can enhance vascular graft design and patency. In blood vessels, they endow vessels with the critical property of elastic recoil. They also influence vascular cell behaviour through direct interactions and by regulating growth factor activation. This review addresses physiological elastic fibre assembly and contributions to vessel structure and function, and how elastic fibre biology is now being exploited in small diameter vascular graft design.

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