Effect of acaricidal components isolated from lettuce (Lactuca sativa) on carmine spider mite (Tetranychus cinnabarinus Boisd.)

2017 ◽  
Vol 108 (3) ◽  
pp. 314-320 ◽  
Author(s):  
M. Li ◽  
Y. Zhang ◽  
W. Ding ◽  
J. Luo ◽  
S. Li ◽  
...  
Keyword(s):  
Lactuca Sativa ◽  
Spider Mite ◽  
Acaricidal Activity ◽  
Post Treatment ◽  
Tetranychus Cinnabarinus ◽  
Liquid Chromatography Mass Spectrometry ◽  
Median Lethal Concentration ◽  
Acetone Extracts ◽  
Layer Chromatography ◽  
High Median

AbstractThis study aimed to evaluate the acaricidal activity of lettuce (Lactuca sativa) extracts against carmine spider mites (Tetranychus cinnabarinus Boisd.) and isolate the acaricidal components. Acaricidal activities of lettuce extracts isolated from different parts (the leaf, root and seed) using various solvents (petroleum ether, acetone and methanol) were evaluated with slide-dip bioassay and relatively high median lethal concentration (LC50) values were detected. Acetone extracts of lettuce leaves harvested in July and September were fractionated and isolated with silica gel and thin-layer chromatography. Consequently, acetone extracts of lettuce leaves harvested in July exhibited higher acaricidal activity than those harvested in September, with an LC50 value of 0.268 mg ml−1 at 72 h post-treatment. A total of 27 fractions were obtained from the acetone extract of lettuce leaves harvested in July, and mite mortalities with the 11th and 12th fractions were higher than those with the other 25 fractions (LC50: 0.751 and 1.258 mg ml−1 at 48 h post-treatment, respectively). Subsequently, active acaricidal components of the 11th fraction were identified by infrared, nuclear magnetic resonance and liquid chromatography/mass spectrometry. Five components were isolated from the 11th fraction, with components 11-a and 11-b showing relatively high acaricidal activities (LC50: 0.288 and 0.114 mg ml−1 at 48 h post-treatment, respectively). Component 11-a was identified as β-sitosterol. In conclusion, acetone extracts of lettuce leaves harvested in July might be used as a novel phytogenic acaricide to control mites.

Synergistic inhibitory effect of scopoletin and bisdemethoxycurcumin on Tetranychus cinnabarinus (Boisduval) (Acari: Tetranychidae)

2016 ◽  
Vol 71 (1-2) ◽  
pp. 1-8 ◽  
Author(s):  
Yong-qiang Zhang ◽  
Zhen-guo Yang ◽  
Wei Ding ◽  
Jin-Xiang Luo
Keyword(s):  
Spider Mite ◽  
Developmental Stages ◽  
Leaf Disc ◽  
Inhibitory Effect ◽  
Synergistic Activity ◽  
Tetranychus Cinnabarinus ◽  
Mass Ratio ◽  
Median Lethal Concentration ◽  
Synergistic Inhibitory Effect ◽  
Spss Software

Abstract The study aimed to investigate the synergistic activity of scopoletin and bisdemethoxycurcumin (BDMC) against the carmine spider mite Tetranychus cinnabarinus. The acaricidal activities of mixtures of scopoletin and BDMC against T. cinnabarinus female adults were measured via slide dipping and leaf disc dipping. A mathematical model was established by SPSS software. Bioassays for multiple effects including contact, ovicidal, cowpea root intake, repellency and oviposition inhibitory activity were carried out. The optimal mass ratio of the mixture of scopoletin and BDMC (at their respective LC50), the median lethal concentration (LC50) and the co-toxicity coefficient were 7:6, 0.19 mg/mL and 129, respectively. LC50 values of contact activities of the mixture at optimal ratio against adults, nymphs, larvae, and eggs were 0.19, 0.18, 0.06, and 1.52 mg/mL, respectively. LC50 values of cowpea root intake activity against adults and nymphs were 5.62 and 6.52 mg/mL, respectively. The highest repellent rates against adults and nymphs were 69.5% and 72.5%, respectively. The mixture of scopoletin and BDMC at the optimal mass ratio possessed strong acaricidal activity against T. cinnabarinus at various developmental stages.

scholarly journals Acaricidal Activity and Synergistic Effect of Thyme Oil Constituents against Carmine Spider Mite (Tetranychus Cinnabarinus (Boisduval))

Molecules ◽  
2017 ◽  
Vol 22 (11) ◽  
pp. 1873 ◽  
Author(s):  
Lipeng Wu ◽  
Xin Huo ◽  
Xiaolong Zhou ◽  
Duoyong Zhao ◽  
Weizhong He ◽  
...  
Keyword(s):  
Synergistic Effect ◽  
Spider Mite ◽  
Acaricidal Activity ◽  
Tetranychus Cinnabarinus ◽  
Thyme Oil ◽  
Carmine Spider Mite

scholarly journals The Acaricidal Activity of Venom from the Jellyfish Nemopilema nomurai against the Carmine Spider Mite Tetranychus cinnabarinus

Toxins ◽  
2016 ◽  
Vol 8 (6) ◽  
pp. 179 ◽  
Author(s):  
Huahua Yu ◽  
Yang Yue ◽  
Xiangli Dong ◽  
Rongfeng Li ◽  
Pengcheng Li
Keyword(s):  
Spider Mite ◽  
Acaricidal Activity ◽  
Tetranychus Cinnabarinus ◽  
Nemopilema Nomurai ◽  
Carmine Spider Mite

Mass spectrometry as a versatile ancillary technique for the rapid in situ identification of lichen metabolites directly from TLC plates

2017 ◽  
Vol 49 (5) ◽  
pp. 507-520 ◽  
Author(s):  
Pierre LE POGAM ◽  
Aline PILLOT ◽  
Françoise LOHEZIC-LE DEVEHAT ◽  
Anne-Cécile LE LAMER ◽  
Béatrice LEGOUIN ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Accurate Identification ◽  
Chemical Profiling ◽  
Analytical Strategies ◽  
Tlc Plates ◽  
Lichen Metabolites ◽  
Acetone Extracts ◽  
Layer Chromatography ◽  
Structural Classes

AbstractThin-layer chromatography (TLC) still enjoys widespread popularity among lichenologists as one of the fastest and simplest analytical strategies, today remaining the primary method of assessing the secondary product content of lichens. The pitfalls associated with this approach are well known as TLC leads to characterizing compounds by comparison with standards rather than properly identifying them, which might lead to erroneous assignments, accounting for the long-held interest in hyphenating TLC with dedicated identification tools. As such, commercially available TLC/Mass Spectrometry (MS) interfaces can be easily connected to any brand of mass spectrometer without adjustments. The spots of interest are extracted from the TLC plate to retrieve mass spectrometric signals within one minute, thereby ensuring accurate identification of the chromatographed substances. The results of this hyphenated strategy for lichens are presented here by 1) describing the TLC migration and direct MS analysis of single lichen metabolites of various structural classes, 2) highlighting it through the chemical profiling of crude acetone extracts of a set of lichens of known chemical composition, and finally 3) applying it to a lichen of unknown profile, Usnea trachycarpa.

Seasonal variation of abscisic acid in the dormant shoots of Douglas-fir

1979 ◽  
Vol 57 (5) ◽  
pp. 534-538 ◽  
Author(s):  
Joe E. Webber ◽  
Murray L. Laver ◽  
Joe B. Zaerr ◽  
Denis P. Lavender
Keyword(s):  
Abscisic Acid ◽  
Liquid Chromatography ◽  
Seasonal Variation ◽  
Internal Standard ◽  
Close Correlation ◽  
Douglas Fir ◽  
Gas Liquid Chromatography ◽  
Bud Burst ◽  
Liquid Chromatography Mass Spectrometry ◽  
Layer Chromatography

The occurrence of abscisic acid (ABA) in the dormant shoots of Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco) was confirmed by bioassay, thin-layer chromatography, gas–liquid chromatography, and gas–liquid chromatography – mass spectrometry. Seasonal variation of ABA in the buds, leaves, and stems was then determined using 2-trans-ABA as an internal standard. Concentrations of ABA were highest in the autumn for buds (2.1 μg/g) and needles (0.79 μg/g) and highest in January for stems (0.34 μg/g). The lowest concentrations for all tissues were in February and March, before bud burst. Close correlation of levels of ABA with previously measured physiological evidence of growth and metabolic activity suggests a possible role in the dormancy cycle of Douglas-fir.

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