scholarly journals Effects of photoperiod and relative humidity on diapause termination and post-winter development of Rhagoletis cerasi pupae

2020 ◽  
Vol 110 (5) ◽  
pp. 588-596
Author(s):  
Cleopatra A. Moraiti ◽  
Kirsten Köppler ◽  
Heidrun Vogt ◽  
Nikos T. Papadopoulos
Keyword(s):  
Relative Humidity ◽  
Pupal Stage ◽  
Adult Emergence ◽  
Fruit Fly ◽  
Light Conditions ◽  
Developmental Duration ◽  
Diapause Termination ◽  
Host Trees ◽  
Chilling Period ◽  
Rhagoletis Cerasi

AbstractThe European cherry fruit fly, Rhagoletis cerasi (Diptera: Tephritidae), is a univoltine species that undergoes obligatory summer–winter diapause at pupal stage in the soil (2–5 cm) beneath host trees. To study the effects of photoperiod and relative humidity on diapause termination and post-winter developmental duration of R. cerasi, pupae collected from Dossenheim (Germany) were exposed to different photoperiod or relative humidity regimes during a chilling period ranging from 2 to 8.5 months. Specifically, pupae were exposed to four photoperiod regimes: (a) light conditions (24L:00D), (b) dark conditions (00L:24D), (c) short photoperiod (08L:16D) and (d) long photoperiod (16L:08D), as well as to three relative humidity regimes: (a) low (40% RH), (b) medium (60% RH) and (c) high (70–80% RH). Data revealed that relative humidity is not a significant predictor of diapause termination, but it affects the post-winter developmental period. Higher relative humidity promotes post-winter pupae development. On the other hand, photoperiod significantly affected both diapause termination and post-winter development of R. cerasi pupae. Light conditions (24L:00D) accelerate adult emergence, particularly for females. Regardless of the photoperiod (24L:00D, 00L:24D, 08L:16D), rates of adult emergence were high (>75%) for chilling intervals longer than 6.5 months. Nonetheless, exposure to a long day photoperiod (16L:08D), during chilling, dramatically reduced the proportion of adult emergence following 6 months exposure to chilling. Our findings broaden the understanding of factors regulating diapause responses in European cherry fruit fly, local adaptation and synchronization of adult emergence with the ripening period of major hosts.

DISINFESTATION OF MANGOES HADEN IN-FESTED BY Anastrepha fraterculus (WIED., 1830) (DIPTERA, TEPHRITIDAE), WITH GAMMA RADIATION

2021 ◽  
Vol 9 (1A) ◽  
Author(s):  
Valter Arthur
Keyword(s):  
Relative Humidity ◽  
Radiation Dose ◽  
Gamma Radiation ◽  
Adult Stage ◽  
Mangifera Indica ◽  
Lethal Dose ◽  
Adult Emergence ◽  
Climate Chamber ◽  
Anastrepha Fraterculus

The objective of experiment was determinate the radiation dose for disinfestation to mango Mangifera indica cv. Haden, infested by Anastrepha fraterculus larvae. For realization of the experiment, were collected fruits in the field, which were taking to Entomology laboratory where there was a infestation by the flies in cages during 72 hours period. Waited for the development of the larvae and before 5 – 8 days to infestation, the mangos were irradiated in a Cobalt-60 source with doses of: 0(control), 50, 100, 200, 300, 400, 500, 600, 700, 800, 900, 1000, 1.100, 1.200 and 1.300 Gy. After the irradiation, the fruits were placed in climate chamber with 25 ±5°C of temperature and 70±5% of relative humidity, posteriorly waited the larvae exit to out of the fruit until the transformation in pupae and posteriorly in adult stage. By the results obtained our can concluded that the lethal dose to larvae in mangoes infested with 5-8 days after infestation were 600 Gy and 1.000 Gy (0.6 and 1 kGy) respectively. The dose of 50 Gy prevented the total adult emergence for both treatments. 

Genetic and cytogenetic analysis of the fruit fly Rhagoletis cerasi (Diptera: Tephritidae)

Genome ◽  
2008 ◽  
Vol 51 (7) ◽  
pp. 479-491 ◽  
Author(s):  
Ilias Kounatidis ◽  
Nikolaos Papadopoulos ◽  
Kostas Bourtzis ◽  
Penelope Mavragani-Tsipidou
Keyword(s):  
Salivary Gland ◽  
Sex Chromosomes ◽  
Cytogenetic Analysis ◽  
Polytene Chromosomes ◽  
Fruit Fly ◽  
Pest Species ◽  
Heteromorphic Sex Chromosomes ◽  
Weak Points ◽  
Heterogametic Sex ◽  
Rhagoletis Cerasi

The European cherry fruit fly, Rhagoletis cerasi , is a major agricultural pest for which biological, genetic, and cytogenetic information is limited. We report here a cytogenetic analysis of 4 natural Greek populations of R. cerasi, all of them infected with the endosymbiotic bacterium Wolbachia pipientis . The mitotic karyotype and detailed photographic maps of the salivary gland polytene chromosomes of this pest species are presented here. The mitotic metaphase complement consists of 6 pairs of chromosomes, including one pair of heteromorphic sex chromosomes, with the male being the heterogametic sex. The analysis of the salivary gland polytene complement has shown a total of 5 long chromosomes (10 polytene arms) that correspond to the 5 autosomes of the mitotic nuclei and a heterochromatic mass corresponding to the sex chromosomes. The most prominent landmarks of each polytene chromosome, the “weak points”, and the unusual asynapsis of homologous pairs of polytene chromosomes at certain regions of the polytene elements are also presented and discussed.

scholarly journals Transcriptome characterization analysis and molecular profiles of obligatory diapause induction of the Chinese citrus fruit fly,Bactrocera minax(Diptera: Tephritidae)

2019 ◽  
Author(s):  
Zhixiong Zhou ◽  
Xiaolin Dong ◽  
Chuanren Li
Keyword(s):  
Signaling Pathway ◽  
Molecular Mechanisms ◽  
Expression Patterns ◽  
Citrus Fruit ◽  
Pupal Stage ◽  
Fruit Fly ◽  
Diapause Induction ◽  
Regulation Mechanism ◽  
Pupal Diapause ◽  
Specific Stage

AbstractThe Chinese citrus fruit fly,Bactrocera minax, is a devastating citrus pest in China, Bhutan and India. It will enter obligatory pupal diapause in each generation at specific stage, while little is known about the course and the molecular mechanisms of diapause induction. To gain insight into possible mechanisms of obligatory pupal diapause induction, high-throughput RNA-seq data were generated from second-instar larvae (2L), third-instar larvae (3L) and pupal (P, one week after pupating). A total of 116,402 unigenes were assembled and researched against public databases, and 54,781 unigenes matched to proteins in the NCBI database using the BLAST search. Three pairwise comparisons were performed, and significantly differentially regulated transcripts were identified. Several differentially expressed genes (DEGs) expression patterns revealed that those highly or lowly expressed genes in pupal stage were predicted to be involved in diapause induction. Moreover, GO function and KEGG pathway analysis were performed on all DEGs and showed that 20-hydroxyecdysone (20E) biosynthesis, insulin signaling pathway, FoxO signaling pathway, cell cycle and metabolism pathway may be related to the obligatory diapause of the Chinese citrus fruit fly. This study provides valuable information about the Chinese citrus fruit fly transcriptome for future gene function research, and contributes to the in-depth elucidation of the molecular regulation mechanism of insect obligatory diapause induction.

scholarly journals Seasonal Abundance and Infestation of Fruit Fly on Cucumber

2021 ◽  
Vol 18 (2) ◽  
pp. 233-241
Author(s):  
S Nahid ◽  
MR Amin ◽  
MM Haque ◽  
SJ Suh
Keyword(s):  
Relative Humidity ◽  
Light Intensity ◽  
Meteorological Factors ◽  
Fruit Fly ◽  
Methyl Eugenol ◽  
Seasonal Abundance ◽  
Bactrocera Cucurbitae ◽  
Larval Population ◽  
Weather Parameters ◽  
Positive Light

Population dynamics and infestation of fruit fly, Bactrocera cucurbitae Coquillett (Diptera: Tephritidae) was monitored on cucumber, Cucumis sativus using methyl eugenol trap during summer and autumn in 2017 at Gazipur, Bangladesh. Relationship between the abundance of fruit fly and meteorological factors was also studied. The fruit fly showed significantly higher abundance in summer compared to autumn. The larval population per fruit was found higher in summer (24.9 fruit-1) than that in autumn (1.5 fruit-1). The daily mean temperature had significant positive, light intensity had insignificant positive, relative humidity had insignificant negative and rainfall had insignificant negative correlation with fruit fly abundance. The temperature, light intensity, relative humidity and rainfall individually contributed to fruit fly abundance of 14.1 %, 24.0 %, 0.8 % and 1.8 %, respectively. The combined effect of the weather parameters on fruit fly abundance was 40.7 % and the equations were insignificant. The fruit fly showed significantly lower level of infestation in methyl eugenol treated plots as compared to controls in both summer and autumn. SAARC J. Agri., 18(2): 233-241 (2020)

scholarly journals Host plant‐related genomic differentiation in the European cherry fruit fly, Rhagoletis cerasi

2019 ◽  
Vol 28 (20) ◽  
pp. 4648-4666 ◽  
Author(s):  
Vid Bakovic ◽  
Hannes Schuler ◽  
Martin Schebeck ◽  
Jeffrey L. Feder ◽  
Christian Stauffer ◽  
...  
Keyword(s):  
Host Plant ◽  
Fruit Fly ◽  
Rhagoletis Cerasi
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