An Assessment of Different Techniques for Inoculating Phaseolus vulgaris with Rhizobium

1984 ◽  
Vol 20 (2) ◽  
pp. 119-127 ◽  
Author(s):  
S. Rawsthorne ◽  
R. J. Summerfield
Keyword(s):  
Phaseolus Vulgaris ◽  
Sucrose Solution ◽  
Dinitrogen Fixation ◽  
Acetylene Reduction Activity ◽  
Rhizobium Phaseoli ◽  
Reduction Activity ◽  
Freeze Dried ◽  
Potential Problems ◽  
Yeast Mannitol Agar ◽  
Pot Culture

SUMMARYA technique to better ensure reliable nodulation of pot-grown, P. vulgaris plants with cultured strains of Rhizobium is described. Nodulation, dinitrogen fixation (acetylene reduction) activity and growth of 28-day-old plants were all stimulated markedly when seeds had been inoculated with cultured Rhizobium phaseoli suspended in alginate gel (Agrigel) rather than in sucrose solution. Nodulation attributes of plants inoculated with R. phaseoli strain RCR 3644 which had been sub-cultured repeatedly on yeast mannitol agar slopes were consistently inferior to those recorded for the same strain but recently cultured from a freeze-dried sample. Some of the potential problems with open pot culture of nodule-dependent legumes are discussed.

An Assessment of Different Techniques for Inoculating Phaseolus vulgaris with Rhizobium

1984 ◽  
Vol 20 (2) ◽  
pp. 119-127
Author(s):  
S. Rawsthorne ◽  
R. J. Summerfield
Keyword(s):  
Phaseolus Vulgaris ◽  
Sucrose Solution ◽  
Dinitrogen Fixation ◽  
Acetylene Reduction Activity ◽  
Rhizobium Phaseoli ◽  
Reduction Activity ◽  
Freeze Dried ◽  
Potential Problems ◽  
Yeast Mannitol Agar ◽  
Pot Culture

SUMMARYA technique to better ensure reliable nodulation of pot-grown, P. vulgaris plants with cultured strains of Rhizobium is described. Nodulation, dinitrogen fixation (acetylene reduction) activity and growth of 28-day-old plants were all stimulated markedly when seeds had been inoculated with cultured Rhizobium phaseoli suspended in alginate gel (Agrigel) rather than in sucrose solution. Nodulation attributes of plants inoculated with R. phaseoli strain RCR 3644 which had been sub-cultured repeatedly on yeast mannitol agar slopes were consistently inferior to those recorded for the same strain but recently cultured from a freeze-dried sample. Some of the potential problems with open pot culture of nodule-dependent legumes are discussed.

Nitrogen fixation associated with 'Park' Kentucky bluegrass (Poapratensis L.)

1979 ◽  
Vol 25 (10) ◽  
pp. 1197-1200 ◽  
Author(s):  
R. C. Shearman ◽  
W. L. Pedersen ◽  
R. V. Klucas ◽  
E. J. Kinbacher
Keyword(s):  
Nitrogen Fixation ◽  
Acetylene Reduction ◽  
Poa Pratensis ◽  
Kentucky Bluegrass ◽  
Acetylene Reduction Activity ◽  
Controlled Environment ◽  
Nitrogen Accumulation ◽  
Reduction Activity ◽  
Significant Difference ◽  
Reduction Assay

Associative nitrogen fixation in Kentucky bluegrass (Poa pratensis L.) turfs inoculated with five nitrogen-fixing bacterial isolates was evaluated using the acetylene reduction assay and nitrogen accumulation as indicators of fixation. 'Park' and 'Nugget' Kentucky bluegrass turfs were grown in controlled environment chambers and inoculated with Klebsiella pneumoniae (W-2, W-6, and W-14), Erwinia herbicola (W-8), and Enterobacter cloacae (W-11). 'Park' inoculated with K. pneumoniae (W-6) had significant acetylene reduction activity using undisturbed turfs. Other treatments including turfs treated with heat-killed cells had no significant difference in acetylene reduction. In a second study, 'Park' and 'South Dakota Certified' turfs were grown in a greenhouse and inoculated with K. pneumoniae (W-6) and E. herbicola (W-8). 'Park' inoculated with K. pneumoniae (W-6) had increased acetylene reduction activity rates and also a greater nitrogen accumulation in aerial tissues when compared to controls. Acetylene reduction activity was correlated (r = 0.92) to nitrogen accumulation. Other treatments did not effectively increase acetylene reduction activity or nitrogen accumulation.

15N-determined dinitrogen fixation potential of genetically diverse bean lines (Phaseolus vulgaris L.)

1989 ◽  
Vol 120 (2) ◽  
pp. 171-179 ◽  
Author(s):  
P. A. A. Pereira ◽  
R. H. Burris ◽  
F. A. Bliss
Keyword(s):  
Phaseolus Vulgaris ◽  
Dinitrogen Fixation ◽  
Phaseolus Vulgaris L

scholarly journals Selection of phytotoxin producing rhizobacteria

2011 ◽  
Vol 83 (3) ◽  
pp. 1091-1096 ◽  
Author(s):  
Daniel D.C. Carvalho ◽  
Denilson F. Oliveira ◽  
Vicente P. Campos ◽  
Moacir Pasqual
Keyword(s):  
Seed Germination ◽  
Sucrose Solution ◽  
Triticum Aestivum L ◽  
In Vitro Assays ◽  
Lettuce Seed ◽  
Bacterial Strains ◽  
Coleoptile Growth ◽  
Liquid Phases ◽  
Freeze Dried

In order to select phytotoxin producing rhizobacteria to control weed plants, twenty five bacterial strains previously isolated from the rhizospheres of various plants were grown in a liquid medium and, after cell removal by centrifugation, the liquid phases were freeze-dried and the products were extracted with ethyl acetate/methanol. The extracts were concentrated to dryness under vacuum and dissolved in water and sucrose solution to be submitted to in vitro assays of lettuce (Lactuca sativa L.) seed germination and wheat (Triticum aestivum L.) coleoptile growth. Although most samples affected coleoptile growth, only those from four strains reduced lettuce seed germination. Two strains of Bacillus cereus, one strain of B. pumilus and one of Stenotrophoonas altophilia were the most promising microorganisms for producing phytotoxin and, consequently, for the development of new weed control products.

Nitrogenase Activity by Subterranean Clover and Other Temperate Pasture Legumes

1988 ◽  
Vol 15 (5) ◽  
pp. 657 ◽  
Author(s):  
AG Davey ◽  
RJ Simpson
Keyword(s):  
Root Respiration ◽  
Nitrogenase Activity ◽  
Subterranean Clover ◽  
Trifolium Subterraneum ◽  
Acetylene Reduction Activity ◽  
Diffusion Resistance ◽  
Pasture Legumes ◽  
Reduction Activity ◽  
Decline Rate ◽  
Nodulated Root

Nitrogenase (C2H2-reduction) activity and nodulated root respiration of intact plants of subterranean clover (Trifolium subterraneum L.) cv. Seaton Park nodulated by Rhizobium trifolii WU95 were measured in a flow-through system. Simultaneous declines in nitrogenase activity and respiration were exhibited 2 min after 10% C2H2 had been introduced into the gas stream. Declines in nitrogenase activity and nodulated root respiration provided an estimate of the efficiency of nitrogenase activity (mol CO2 evolved/mol C2H4 produced). The pre-decline rate of nitrogenase activity at time zero was thus calculated as the product of the respiration associated with nitrogenase activity and the reciprocal of the efficiency of nitrogenase activity. Pre-decline rates of nitrogenase activity were similar to peak rates for several pasture legumes. However, post-decline rates of activity were as much as 70% lower than the pre-decline rate. The age of subterranean clover plants had an important influence on the magnitude of the C2H2-induced decline; young plants exhibited the largest C2H2-induced inhibition of nitrogenase activity. Neither sainfoin (Onobrychis viciifolia Scop.) cv. Othello nodulated by Rhizobium sp. CC1108 nor yellow serradella (Ornithopus compressus L.) cv. Pitman nodulated by R. lupini WU425 exhibited C2H2-induced declines in nitrogenase activity. Nitrogenase-linked respiration of subterranean clover at the 14-leaf stage accounted for 50% of total nodulated root respiration. The oxygen diffusion resistance of the nodules increased in the presence of C2H2 but the effect was reversible once C2H2 was removed from the gas atmosphere. The pre-decline rate of acetylene reduction activity of subterranean clover reached a maximum at 10% C2H2. The C2H2-induced decline in nitrogenase activity was lower at subsaturating pC2H2 and was not detected at 0.4% C2H2.

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