The effects of cultivation method, fertilizer input and previous sward type on organic C and N storage and gaseous losses under spring and winter barley following long-term leys

2002 ◽  
Vol 139 (3) ◽  
pp. 231-243 ◽  
Author(s):  
A. J. A. VINTEN ◽  
B. C. BALL ◽  
M. F. O'SULLIVAN ◽  
J. K. HENSHALL
Keyword(s):  
Spring Barley ◽  
Balance Sheet ◽  
N Fertilizer ◽  
Net N Mineralization ◽  
No Tillage ◽  
Confidence Limits ◽  
Organic C ◽  
Soil C ◽  
C And N

The effects of ploughing or no-tillage of long-term grass and grass-clover swards on changes in organic C and N pools and on CO2 and denitrified gas emissions were investigated in a 3-year field experiment in 1996–99 near Penicuik, Scotland. The decrease in soil C content between 1996 and 1999 was 15·3 t/ha (95% confidence limits were 1·7–28·9 t/ha). Field estimates of CO2 losses from deep-ploughed, normal-ploughed and no-tillage plots were 3·1, 4·5 and 4·6 t/ha over the sampling periods (a total of 257 days) in 1996–98. The highest N2O fluxes were from the fertilized spring barley under no-tillage. Thus no-tillage did not reduce C emissions, caused higher N2O emissions, and required larger inputs of N fertilizer than ploughing. By contrast, deep ploughing led to smaller C and N2O emissions but had no effect on yields, suggesting that deep ploughing might be an appropriate means of conserving C and N when leys are ploughed in. Subsoil denitrification losses were estimated to be 10–16 kg N/ha per year by measurement of 15N emissions from incubated intact cores. A balance sheet of N inputs and outputs showed that net N mineralization over 3 years was lower from plots receiving N fertilizer than from plots receiving no fertilizer.

scholarly journals Linking Cover Crop Residue Quality and Tillage System to CO2-C Emission, Soil C and N Stocks and Crop Yield Based on a Long-Term Experiment

Agronomy ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 1848
Author(s):  
Otávio A. Leal ◽  
Telmo J. C. Amado ◽  
Jackson E. Fiorin ◽  
Cristiano Keller ◽  
Geovane B. Reimche ◽  
...  
Keyword(s):  
Crop Yield ◽  
Cover Crops ◽  
No Tillage ◽  
Yield Data ◽  
Soil C ◽  
Term Experiment ◽  
Long Term Experiment ◽  
Soil C And N ◽  
C And N

Cover crops (CC), particularly legumes, are key to promote soil carbon (C) sequestration in no-tillage. Nevertheless, the mechanisms regulating this process need further elucidation within a broad comprehensive framework. Therefore, we investigated effects of CC quality: black oat (Avena strigosa Schreb) (oat), common vetch (Vicia sativa L.) (vetch), and oat + vetch on carbon dioxide-C (CO2-C) emission (124 days) under conventional- (CT), minimum- (MT) and no-tillage (NT) plots from a long-term experiment in Southern Brazil. Half-life time (t1/2) of CC residues and the apparent C balance (ACB) were obtained for CT and NT. We linked our data to long-term (22 years) soil C and nitrogen (N) stocks and crop yield data of our experimental field. Compared to CT, NT increased t1/2 of oat, oat + vetch and vetch by 3.9-, 3.1- and 3-fold, respectively; reduced CO2-C emissions in oat, oat + vetch and vetch by 500, 600 and 642 kg ha−1, respectively; and increased the ACB (influx) in oat + vetch (195%) and vetch (207%). For vetch, CO2-C emission in MT was 77% greater than NT. Legume CC should be preferentially combined with NT to reduce CO2-C emissions and avoid a flush of N into the soil. The legume based-NT system showed the greatest soil C and N sequestration rates, which were significantly and positively related to soybean (Glycine max (L.) Merrill) and maize (Zea mays L.) yield. Soil C (0–90 cm depth) and N (0–100 cm depth) sequestration increments of 1 kg ha−1 corresponded to soybean yield increments of 1.2 and 7.4 kg ha−1, respectively.

Management effects on the dynamics and storage rates of organic matter in long-term crop rotations

2004 ◽  
Vol 84 (1) ◽  
pp. 49-61 ◽  
Author(s):  
E. A. Paul ◽  
H. P. Collins ◽  
K. Paustian ◽  
E. T. Elliott ◽  
S. Frey ◽  
...  
Keyword(s):  
Organic Matter ◽  
C Sequestration ◽  
Organic C ◽  
Soil C ◽  
Site Analysis ◽  
Laboratory Incubation ◽  
C And N ◽  
A Site ◽  
Management Effects

Factors controlling soil organic matter (SOM) dynamics in soil C sequestration and N fertility were determined from multi-site analysis of long-term, crop rotation experiments in Western Canada. Analyses included bulk density, organic and inorganic C and N, particulate organic C (POM-C) and N (POM -N), and CO2-C evolved during laboratory incubation. The POM-C and POM-N contents varied with soil type. Differences in POM-C contents between treatments at a site (δPOM-C) were related (r2= 0.68) to treatment differences in soil C (δSOC). The CO2-C, evolved during laboratory incubation, was the most sensitive indicator of management effects. The Gray Luvisol (Breton, AB) cultivated plots had a fivefold difference in CO2-C release relative to a twofold difference in soil organic carbon (SOC). Soils from cropped, Black Chernozems (Melfort and Indian Head, SK) and Dark Brown Chernozems (Lethbridge, AB) released 50 to 60% as much CO2-C as grassland soils. Differences in CO2 evolution from the treatment with the lowest SOM on a site and that of other treatments (δCO2-C) in the early stages of the incubation were correlated to δPOM-C and this pool reflects short-term SOC storage. Management for soil fertility, such as N release, may differ from management for C sequestration. Key words: Multi-site analysis, soil management, soil C and N, POM-C and N, CO2 evolution

scholarly journals Long-Term Impact of Liming on Soil C and N in a Fertile Spruce Forest Ecosystem

Ecosystems ◽  
2020 ◽  
Author(s):  
T. Persson ◽  
S. Andersson ◽  
J. Bergholm ◽  
T. Grönqvist ◽  
L. Högbom ◽  
...  
Keyword(s):  
Forest Soils ◽  
Heterotrophic Respiration ◽  
Organic Layer ◽  
N Loss ◽  
Organic C ◽  
Soil C ◽  
Soil C And N ◽  
C And N ◽  
C And N Pools

Abstract Liming can counteract acidification in forest soils, but the effects on soil C and N pools and fluxes over long periods are less well understood. Replicated plots in an acidic and N-rich 40-year-old Norway spruce (Picea abies) forest in SW Sweden (Hasslöv) were treated with 0, 3.45 and 8.75 Mg ha−1 of dolomitic lime (D0, D2 and D3) in 1984. Between 1984 and 2016, soil organic C to 30 cm depth increased by 28 Mg ha−1 (30% increase) in D0 and decreased by 9 Mg ha−1 (9.4% decrease) in D3. The change in D2 was not significant (+ 2 Mg ha−1). Soil N pools changed proportionally to those in soil C pools. The C and N changes occurred almost exclusively in the top organic layer. Non-burrowing earthworms responded positively to liming and stimulated heterotrophic respiration in this layer in both D2 and D3. Burrowing earthworms in D3 further accelerated C and N turnover and loss of soil. The high soil C and N loss at our relatively N-rich site differs from studies of N-poor sites showing no C and N loss. Earthworms need both high pH and N-rich food to reach high abundance and biomass. This can explain why liming of N-rich soils often results in decreasing C and N pools, whereas liming of N-poor soils with few earthworms will not show any change in soil C and N. Extractable nitrate N was always higher in D3 than in D2 and D0. After 6 years (1990), potential nitrification was much higher in D3 (197 kg N ha−1) than in D0 (36 kg N ha−1), but this difference decreased during the following years, when also the unlimed organic layers showed high nitrification potential. Our experiment finds that high-dose liming of acidic N-rich forest soils produces an initial pulse of soil heterotrophic respiration and increases in earthworm biomass, which together cause long-term declines in soil C and N pools.

EFFECT OF FERTILIZER ON SOIL PRODUCTIVITY IN LONG-TERM SPRING WHEAT ROTATIONS

1987 ◽  
Vol 67 (1) ◽  
pp. 165-174 ◽  
Author(s):  
H. H. JANZEN
Keyword(s):  
Organic Matter ◽  
N Fertilization ◽  
N Fertilizer ◽  
Organic N ◽  
Phosphorus Fertilization ◽  
Soil Productivity ◽  
Organic C ◽  
C And N ◽  
N Fertility

Surface soil samples taken from two long-term crop rotations at Lethbridge, Alberta were used to assess the influence of fertilizer N and P on total and mineralizable concentrations of organic C and N in a Dark Brown Chernozemic soil. Rotations sampled were continuous wheat and fallow-wheat-wheat initiated in 1912. In 1967 and 1972, N fertilizer and P fertilizer treatments, respectively, were superimposed over the rotation treatments (which had received no previous fertilizer) to produce a factorial of two N rates (0 and 45 kg N ha−1) by two P rates (0 and 20 kg P ha−1). After 18 yr of application, N fertilizers increased organic C content in both rotations by approximately 14% over that observed in soil receiving no N fertilizer. Organic N contents, similarly, were increased by 15 and 11% in the continuous wheat and fallow-wheat-wheat rotations, respectively. As well, N fertilization increased relative N mineralization potential by 22% in the continuous wheat rotation and by 44% in the fallow-wheat-wheat rotation. Phosphorus fertilization had no significant influence on either total or mineralizable C and N concentrations. Soil pH (measured in dilute CaCl2) was reduced, on average, from 7.2 to 6.9 by 18 annual N applications. These results demonstrate that N fertilization can make significant contributions to the replenishment of organic matter in soil and to the maintenance of indigenous soil N fertility. Key words: Organic matter, nitrogen, carbon, mineralizable nitrogen, mineralizable carbon, pH

scholarly journals Changes in soil organic matter under different land management in misiones province (Argentina)

2008 ◽  
Vol 65 (3) ◽  
pp. 290-297 ◽  
Author(s):  
Gabriel Agustín Piccolo ◽  
Adrián Enrique Andriulo ◽  
Bruno Mary
Keyword(s):  
Cover Crop ◽  
Native Forest ◽  
Yerba Mate ◽  
No Tillage ◽  
Elephant Grass ◽  
Organic C ◽  
Soil C ◽  
Soil C And N ◽  
C And N ◽  
13C Abundance

Highly weathered tropical soils rapidly loose soil organic matter (SOM) and may be affected by water erosion and soil compaction after deforestation and intensive cultivation. With the main objective to estimate the SOM balances in a subtropical soil we determined the dynamics of SOM in a degraded yerba mate (Ilex paraguaiensis Saint Hil.) plantation introduced after deforestation and with elephant grass (Pennisetum purpureum L.) as a cover crop. The study site was in Misiones, Argentina, and we use the natural 13C abundance methodology and a descriptive model. The study was conducted on three contiguous 50 x 100 m plots of a typic Kandihumult soil with: (i) native forest, (ii) 50 years of continuous yerba mate monoculture with intensive tillage, and (iii) yerba mate associated with elephant grass as a cover crop and no tillage. We determined bulk density, carbon (C), nitrogen (N) and 13C content of the soil (0 - 0.05, 0.05 - 0.15 m layers) and the grass biomass. Yerba mate monoculture reduced soil C and N content as well as porosity at 0 - 0.15 m depth by 43 and 23%, respectively, as compared to the native forest. After ten years of yerba mate - elephant grass association soil C and N contents at the same depth increased by 19 and 12%, respectively, compared to the yerba mate monoculture, while soil porosity remained similar. Total C input,13C, and soil organic C were incorporated into a three compartment model to evaluate elephant grass C dynamics. Through the natural 13C abundance methodology we tracked the elephant grass C incorporation and the "old" soil C loss, and determined the model parameters - humification (k1) and mineralization (k) coefficients and stable C (Cs)- unambiguously. The high k1 and k predicted by the model are probably explained by elephant grass root system incorporation under no tillage and humid subtropical climate, respectively. In soil under yerba mate monoculture, Cs was counted as 91% of the total soil organic C.

scholarly journals Long-Term No-Tillage and Straw Retention Management Enhances Soil Bacterial Community Diversity and Soil Properties in Southern China

Agronomy ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1233
Author(s):  
Yuqiong Luo ◽  
Anas Iqbal ◽  
Liang He ◽  
Quan Zhao ◽  
Shangqin Wei ◽  
...  
Keyword(s):  
Soil Properties ◽  
Conventional Tillage ◽  
No Tillage ◽  
Total N ◽  
Organic C ◽  
Soil C ◽  
Tillage Practices ◽  
Straw Retention ◽  
Soil Bacterial

Conservation farming practices, such as no-tillage and crop residue retention, have been proposed as sustainable management practices. However, it remains unclear how different tillage practices and rice straw retention affect the soil bacterial community (SBC) and the soil C/N ratio in the long term. The objective of this study was to evaluate changes in SBC composition and abundance and soil properties (e.g., carbon (C), nitrogen (N)) and determine their relationship to the soil C/N ratio under long-term no-tillage and straw retention techniques. This study investigates the effect of a long-term field experiment begun in 2008 and continued until 2019 to measure the response of the SBC and soil properties and their relation to different tillage practices, including no-tillage (NT), no-tillage and straw mulching (NT-SM), conventional tillage (CT), conventional tillage and straw mulching (CT-SM), and conventional tillage and straw retention (CT-SR). Soil samples were collected at depths of 0–5 cm (A), 5–10 cm (B), and 10–20 cm (C) after rice harvesting in the early and late growing seasons in 2018–2019. The Illumina MiSeq sequencing and quantitative polymerase chain reaction (PCR) technology was used to analyze changes in SBC diversity in soil and determined the changes in the soil C/N ratio and their relationship with the SBC diversity. The results showed that the Proteobacteria, Acidobacteria, and Chloroflexi were the dominant phyla in the soil and accounted for 61.26%, 59.39%, and 55.62% of the total bacteria in the A, B, and C soil layers, respectively. The NT treatment increased SBC diversity, the number of operational taxonomic units (OTUs), and the proportion of Proteobacteria across the soil depths. Similarly, straw retention also significantly improved SBC diversity, soil organic C (SOC), total N (TN), soil C/N ratio, and the abundance of Proteobacteria and Acidobacteria in the soil layers A and B. The NT-SM treatment increased the SOC, TN, and soil C/N ratio by 30%, 21%, and 6% in 2018 and by 33, 25% and 7% in 2019, respectively, across the seasons and layers compared to the CT treatment. The NT-SM treatment had the highest soil bacterial diversity index, and the CT-SR treatment had the highest soil bacterial abundance and number of OTUs. The redundancy analysis showed that Acidobacteria were highly positively correlated with the soil C/N ratio. The results demonstrate that conservation tillage practices, i.e., no-tillage and straw retention, increase the SBC diversity and soil C/N ratio, thereby enhancing soil organic C and total N and changing soil microbial ecology. As a result, sustainable crop production and profitable agro-ecosystems are ensured.

Effect of crop management on C and N in long-term crop rotations after adopting no-tillage management: Comparison of soil sampling strategies

1998 ◽  
Vol 78 (1) ◽  
pp. 155-162 ◽  
Author(s):  
C. A. Campbell ◽  
F. Selles ◽  
G. P. Lafond ◽  
B. G. McConkey ◽  
D. Hahn
Keyword(s):  
Organic Matter ◽  
Crop Rotations ◽  
No Tillage ◽  
Negative Consequences ◽  
Total N ◽  
Organic C ◽  
Soil C ◽  
Soil C And N ◽  
C And N ◽  
Over Time

Society is interested in increasing C storage in soil to reduce CO2 concentration in the atmosphere, because the latter may contribute to global warming. Further, there is considerable interest in the use of straw for industrial purposes. Using soil samples taken from the 0- to 7.5-cm and 7.5- to 15-cm depths in May 1987 and September 1996, we determined organic C and total N in five crop rotations (nine treatments) using automated Carlo Erba combustion analyzer. The experiment was managed using conventional mechanical tillage from 1957 to 1989; it was changed to no-tillage management in 1990. Our objective was to determine: (a) if change to no-tillage management had changed soil C and N storage, and (b) if method of calculating organic C and N change would influence interpretation of the results. All three methods of calculation confirmed the efficacy of employing best management practices (e.g., fertilization based on soil tests, reducing summerfallow, including legumes in rotations) for increasing or maintaining soil organic matter, and showed that the latter was directly associated with the amount of crop residues returned to the soil. Where bulk density was significantly different between sampling times, the often used mass per fixed depth (MFD) (i.e., volume basis) calculation can lead to erroneous conclusions. When the recently recommended mass per equal depth (MED) method of calculation was used, it showed that 6 yr of no-tillage did not increase soil organic C or total N. However, in unfertilized systems, where crop yields are gradually decreasing since the change, there is an accompanying decrease in organic matter, while fertilized, or high-fertility systems that include legume hay crops, in which wheat yields have been maintained have tended to maintain the organic matter level over time. When the MFD calculation was used, there was no change in C over time when straw was harvested in the F–W–W system; however, the MED calculation and concentrations tend to show a decrease in soil C and N. This suggests that in time, industrial use of straw may have negative consequences for soil conservation. We concluded that concentrations may be as effective as MED for assessing changes in organic matter, provided "amounts" are not required. Key words: Straw removal, fertilizers, legumes, cropping frequency, C mass calculation

The effects of cultivation method and timing, previous sward and fertilizer level on subsequent crop yields and nitrate leaching following cultivation of long-term grazed grass and grass-clover swards

2002 ◽  
Vol 139 (3) ◽  
pp. 245-256 ◽  
Author(s):  
A. J. A. VINTEN ◽  
B. C. BALL ◽  
M. F. O'SULLIVAN ◽  
J. K. HENSHALL ◽  
R. HOWARD ◽  
...  
Keyword(s):  
Grain Yield ◽  
Nitrate Leaching ◽  
Spring Barley ◽  
N Uptake ◽  
Barley Grain ◽  
Winter Barley ◽  
N Fertilizer ◽  
No Tillage ◽  
Fertilizer N

A 3-year field experiment was conducted in 1996–98 near Penicuik, Scotland, to investigate the fate of N released after cultivation of previously long-term grass and grass-clover swards. The effects of timing of cultivations (autumn and spring), tillage methods (no tillage, ploughing to 200 mm and ploughing to 300 mm) and fertilizer N for spring (0, 40, 80 and 120 kg N/ha) and winter barley (0, 60, 120, 180 kg N/ha) on yield, N uptake and nitrate leaching were measured.In 1996, after spring cultivation, on plots previously in grass, spring barley grain yield and N uptake did not respond to N fertilizer, but on plots previously in grass-clover there was a nearly linear response to N. In 1997, the spring barley responded to N fertilizer at all levels. Yields of 1997 winter barley after grass did not show a response above 60 kg N/ha, but increased with fertilizer N up to at least 120 kg N/ha after grass-clover. In 1998, there were strong effects of N fertilizer and cultivation method on grain yield and N uptake of both spring barley and winter barley. Winter barley grain yield was significantly higher in plots previously in grass than in plots previously in grass-clover in 1998, though not in 1997. Winter barley yields were higher than spring barley at the same fertilizer N level.Throughout the 3 years, the no-tillage plots had consistently lower yields than the ploughed plots, but there was no consistent difference between the ploughed and deep ploughed treatments. There were strong interactive effects between tillage and previous sward in 1997. No-tillage under spring barley generally yielded lower than ploughing due to difficulties in weed control and the frequent anaerobic conditions in the soil.Annual leaching losses were relatively small (6·4–19·6 kg N/ha). In 1996–97, more N was leached from the plots left in stubble following spring barley than from those planted with winter barley after either spring barley or grass in 1996, but in 1997–98 more N was leached from plots in winter barley than from those in over-winter stubble. Nitrate leaching was least under no-tillage, though the effect was not significant.

Long-term effects of crop rotations and fertilization on soil C and N in a thin Black Chernozem in southeastern Saskatchewan

2012 ◽  
Vol 92 (3) ◽  
pp. 449-461 ◽  
Author(s):  
R.L. Lemke ◽  
A.J. Vandenbygaart ◽  
C.A. Campbell ◽  
G.P. Lafond ◽  
B.G. McConkey ◽  
...  
Keyword(s):  
Carbon Sequestration ◽  
Organic Carbon ◽  
Crop Rotations ◽  
No Tillage ◽  
Long Term Effects ◽  
Soil C ◽  
No Till ◽  
Soil C And N ◽  
C And N

Lemke, R. L., VandenBygaart, A. J., Campbell, C. A., Lafond, G. P., McConkey, B. G. and Grant, B. 2012. Long-term effects of crop rotations and fertilization on soil C and N in a thin Black Chernozem in southeastern Saskatchewan. Can. J. Soil Sci. 92: 449–461. Carbon sequestration in soil is important due to its influence on soil fertility and its impact on the greenhouse gas (GHG) phenomenon. Carbon sequestration is influenced by agronomic factors, but to what extent is still being studied. Long-term agronomic studies provide one of the best means of making such assessments. In this paper we discuss and quantify the effect of cropping frequency, fertilization, legume green manure (LGM) and hay crops in rotations, and tillage on soil organic carbon (SOC) changes in a thin Black Chernozemic fine-textured soil in southeastern Saskatchewan. This was based on a 50-yr (1958–2007) crop rotation experiment which was initiated on land that had previously been in fallow-wheat (Triticum aestivum L.) (F-W), or F-W-W receiving minimum fertilizer for the previous 50 yr. We sampled soil in 1987, 1996 (6 yr after changing from conventional tillage to no-tillage management and increasing N rates markedly) and again in 2007. The SOC (0–15 cm depth) in unfertilized F-W and F-W-W appears not to have changed from the assumed starting level, even after 20 yr of no-till, but SOC in unfertilized continuous wheat (Cont W) increased slightly [not significant (P>0.05)] in 30 yr, but increased more after 20 yr of no-till (but still not significant). No-till plus proper fertilization for 20 yr increased the SOC of F-W, F-W-W and Cont W in direct proportion to cropping frequency. The SOC in the LGM-W-W (unfertilized) system was higher than unfertilized F-W-W in 1987, but 20 yr of no-tillage had no effect, likely because grain yields and C inputs were depressed by inadequate available P. Soil organic carbon in the two aggrading systems [Cont W (N+P) and F-W-W-hay(H)-H-H (unfertilized)] increased significantly (P<0.05) in the first 30 yr; however, a further 20 yr of no-tillage (and increased N in the case of the Cont W) did not increase SOC suggesting that the SOC had reached a steady-state for this soil and management system. The Campbell model effectively simulated SOC changes except for Cont W(N+P), which it overestimated because the model is ineffective in simulating SOC in very fertile systems. After 50 yr, efficiency of conversion of residue C inputs to SOC was negligible for unfertilized F-W and F-W-W, was 3 to 4% for fertilized fallow-containing systems, was about 6 or 7% for Cont W, and about 11% for the unfertilized F-W-W-H-H-H systems.

Manipulation of rhizosphere organisms to enhance glomalin production and C sequestration: Pitfalls and promises

2014 ◽  
Vol 94 (6) ◽  
pp. 1025-1032 ◽  
Author(s):  
F. L. Walley ◽  
A. W. Gillespie ◽  
Adekunbi B. Adetona ◽  
J. J. Germida ◽  
R. E. Farrell
Keyword(s):  
Plant Growth ◽  
Mycorrhizal Fungi ◽  
Plant Growth Promoting Rhizobacteria ◽  
C Sequestration ◽  
Ionization Mass ◽  
Edge Structure ◽  
Organic C ◽  
Soil C ◽  
N Storage ◽  
C And N

Walley, F. L., Gillespie, A. W., Adetona, A. B., Germida, J. J. and Farrell, R. E. 2014. Manipulation of rhizosphere organisms to enhance glomalin production and C-sequestration: Pitfalls and promises. Can. J. Plant Sci. 94: 1025–1032. Arbuscular mycorrhizal fungi (AMF) reportedly produce glomalin, a glycoprotein that has the potential to increase soil carbon (C) and nitrogen (N) storage. We hypothesized that interactions between rhizosphere microorganisms, such as plant growth-promoting rhizobacteria (PGPR), and AMF, would influence glomalin production. Our objectives were to determine the effects of AMF/PGPR interactions on plant growth and glomalin production in the rhizosphere of pea (Pisum sativum L.) with the goal of enhancing C and N storage in the rhizosphere. One component of the study focussed on the molecular characterization of glomalin and glomalin-related soil protein (GRSP) using complementary synchrotron-based N and C X-ray absorption near-edge structure (XANES) spectroscopy, pyrolysis field ionization mass spectrometry (Py-FIMS), and proteomics techniques to characterize specific organic C and N fractions associated with glomalin production. Our research ultimately led us to conclude that the proteinaceous material extracted, and characterized in the literature, as GRSP is not exclusively of AMF origin. Our research supports the established concept that GRSP is important to soil quality, and C and N storage, irrespective of origin. However, efforts to manipulate this important soil C pool will remain compromised until we more clearly elucidate the chemical nature and origin of this resource.

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