Evidence of selective changes in winter wheat in middle-European environments reflected by allelic diversity at loci affecting plant height and photoperiodic response

2010 ◽  
Vol 149 (3) ◽  
pp. 313-326 ◽  
Author(s):  
V. ŠÍP ◽  
J. CHRPOVÁ ◽  
A. ŽOFAJOVÁ ◽  
Z. MILEC ◽  
D. MIHALIK ◽  
...  

SUMMARYGenes for adaptation to climatic conditions can have an impact on the expression of genes for agricultural productivity. This study tested the hypothesis that winter wheat cultivars registered in middle Europe (especially the Czech and Slovak Republics) during the period 1976–2009 were differentially adapted to different regions, reflecting selection for different allelic combinations. This was tested by analysing for the presence of alleles at the Rht and Ppd loci using molecular markers and gibberellic acids (GA) response tests. Four allelic variants (174, 192, 165 and 198-bp) were detected at the Xgwm261 locus linked to Rht8 on chromosome 2D. The 198-bp allele was rare, but present in some of the most widely grown cultivars. Of 85 cultivars grown in the area of Czech Republic, the 174-bp allele predominated in frequency and area (39 cultivars), often in combination with Ppd-D1b (30 out of 39 cultivars) and Rht-D1b (15 out of 30 cultivars). In neighbouring Slovakia, the 192-bp allele, generally associated with Ppd-D1a, was detected in 30 out of 40 cultivars; in 12 cultivars accompanied by a GA-insensitive allele on 4B chromosome (pedigree analyses indicated a high prevalence of the Rht-B1d allele). The 192-bp (Rht8)/Ppd-D1a linkage block was broken up in 7 out of 22 cultivars that carried the 192-bp allele in the ‘Czech collection’. Analysis of the effects of year of registration on allele frequency showed a decline in GA-insensitive cultivars released recently in both countries, and great changes in the frequency of the 2D alleles during the period 1981–2009 in the Czech Republic. The pedigrees of successful cultivars were examined to find probable sources of Xgwm261 192-bp, 174-bp and 165-bp alleles on 2D and Rht genes located on chromosomes 4B and 4D. These results will impact on breeding strategies and the exploitation of existing registered wheat cultivars in different regions and growing systems.

Euphytica ◽  
2009 ◽  
Vol 172 (2) ◽  
pp. 221-233 ◽  
Author(s):  
Václav Šíp ◽  
Jana Chrpová ◽  
Alžběta Žofajová ◽  
Kateřina Pánková ◽  
Martin Užík ◽  
...  

2017 ◽  
Vol 63 (No. 7) ◽  
pp. 315-321
Author(s):  
Phuong LE Minh ◽  
Lachman Jaromír ◽  
KOTÍKOVÁ Zora ◽  
Orsák Matyáš ◽  
MICHLOVÁ Tereza ◽  
...  

Eighteen winter wheat cultivars with different grain colour (purple-, blue-, yellow- and red-grained) and three spring tritordeum yellow-grained cultivars and breeding lines were assessed for grain selenium (Se) content from the crop season 2014/2015 on the experimental field Agrotest fyto, Ltd., Kroměříž (Czech Republic). Se content has shown to be genotype dependent, with the highest contents in control red-grained cv. Bohemia (0.235 mg/kg dry matter (DM)) and yellow-grained cv. Bona Vita (0.229 mg/kg DM), and breeding lines V2 10–16 (blue-grained), KM 53–14 (blue-grained) and V2 15–16 (yellow-grained) winter wheats. In new spring tritordeums, average Se content was comparable (0.039 mg/kg DM) with purple pericarp wheats (0.042 mg/kg DM); in wheats with blue aleurone and yellow endosperm it was higher (0.057 mg/kg DM and 0.069 mg/kg DM). Although in most cultivars the Se contents were not significantly different, statistically significant differences were determined between the cvs. Bohemia and Bona Vita with the highest Se content and breeding line V2 31–16 with the lowest Se content as well as between the cv. Bohemia and breeding line KM 178–14. Grain colour of wheat cultivars and breeding lines affected Se content, so possible wheat genetic resources for use in the breeding process can be assessed. Diversity in certain wheat accessions offers genetic potential for developing cultivars with better ability to accumulate beneficial Se micronutrient in grains.


2010 ◽  
Vol 42 (No. 1) ◽  
pp. 21-25 ◽  
Author(s):  
M. Váňová ◽  
P. Matušinský ◽  
J. Benada

Bunts (caused by <i>Tilletia caries</i> and <i>T. controversa</i>) belong to very important diseases of winter wheat because contaminated commodities (seeds, foods and feeds) affect the marketability of the crop on both domestic and export markets. They can be relatively easily controlled by chemical seed treatments. Due to the availability of effective chemical control, the reaction of wheat cultivars to bunts has so far not been an important trait for plant breeders in some areas of the world. However, if synthetic chemicals are not allowed, like in organic farming, untreated seed may quickly lead to a build-up of bunt to levels that render the crop unmarketable. The use of wheat cultivars partially or fully resistant to bunts could greatly contribute to ease the bunt problem. The reaction of winter wheat cultivars was evaluated in field tests. Seeds of winter wheat were inoculated with teliospores of <i>T. caries</i>. The reaction to <i>T. controversa</i> was studied under heavy natural infestation with spores in the soil. With <i>T. caries</i>, the heaviest infection was found in cvs Drifter and Ebi, while cvs Nela, Brea and Samanta had the lowest. The average level of infection with <i>T. controversa</i> was higher than that of <i>T. caries</i>. The cvs Niagara, Brea and Versailles had significantly lower numbers of bunt ears of <i>T. controversa</i> in 2002. The incidence of both bunts in grain samples that had not been cleaned and sorted after harvest was monitored for 4 years. A total of 1 058 samples collected from various locations in the Czech Republic were analysed for the presence of bunt spores and the species determined. The investigation demonstrated a rather widespread occurrence of bunts across the Czech Republic, with <i>T. controversa</i> being more frequent.


2011 ◽  
Vol 42 (No. 2) ◽  
pp. 31-37
Author(s):  
J. Palicová-Šárová ◽  
A. Hanzalová

The reaction of 50 winter wheat cultivars/lines to artificial infection with Pyrenophora tritici-repentis (PTR) races 1, 3, and 6 was studied under greenhouse conditions. The set of tested cultivars/lines included predominantly cultivars registered in the Czech Republic and some new breeding lines. A high level of resistance to P. tritici-repentis was detected in the cultivars Clarus, Rheia, Cubus, SHMK WW 14-92, &Scaron;&aacute;rka, Vlasta and Dromos (SWS 799.14953), susceptible reactions were observed in the cultivars Caphorn, Corsaire, Karolinum, Heroldo (PBIS 00/91), Hedvika, Biscay, Svitava, Barroko (PBIS 00/140) to all three races tested. The majority of the tested cultivars possess a moderate level of resistance to PTRraces 1, 3, and 6. Significant differences were proved not only in the reaction of the tested cultivars but also in the aggressiveness of the three used isolates. &nbsp;


2017 ◽  
Vol 38 (SI 2 - 6th Conf EFPP 2002) ◽  
pp. 575-579
Author(s):  
J. Šárová ◽  
V. Šíp ◽  
A. Hanzalová

The response of 22 winter wheat cultivars to artificial infection with Pyrenophora tritici-repentis has been studied in the field conditions for three years and in the greenhouse conditions as well. The set of cultivars included the cultivars registered in the Czech Republic and Swiss cultivar Arina known for satisfactory resistance to different foliar and head blights. High level of field resistance to Pyrenophora tritici-repentis was detected in cultivars Arina, Vlasta, Rialto, Athlet, Trane, Siria, Vega, Alana, Samara. Not all cultivars showed similar reaction in field and greenhouse tests. Correlation between results of the field and the greenhouse tests was significant for 18 of 22 cultivars. Arina, Vlasta and Vega showed acceptable field resistance and high resistance in the greenhouse tests to Pyrenophora tritici-repentis too. The greenhouse experiments helped to differentiate between examined isolates collected from the territory of the Czech Republic. Cultivar reaction to isolates No. 98001, 98007, 98010, 00071 correlated significantly with the field results.


2017 ◽  
Vol 54 (No. 1) ◽  
pp. 24-30
Author(s):  
Palicová Jana ◽  
Matušinsky Pavel ◽  
Dumalasová Veronika ◽  
Hanzalová Alena ◽  
Bížová Irena

The reaction of ten winter wheat cultivars grown in the Czech Republic to inoculation with Oculimacula yallundae and Oculimacula acuformis was evaluated in a small plot trial. In a parallel field trial the natural occurrence of stem-base disease complex in six of the tested cultivars was assessed. Lower severity of eyespot (and/or stem-base diseases) was observed in cultivars possessing the resistance gene Pch1 (cvs Hermann, Annie, Princeps, Manager, and Rebell) in plots inoculated with Oculimacula spp. as well as in natural field conditions. A total of 468 wheat stem bases from the Czech Republic was screened by PCR to study the frequency of eyespot causal agents. The plants were colonised significantly more often by both species Oculimacula yallundae and O. acuformis together than separately.


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