scholarly journals Barley seed coating with free and immobilized alkaline phosphatase to improve P uptake and plant growth

2012 ◽  
Vol 150 (6) ◽  
pp. 691-701 ◽  
Author(s):  
MARÍA C. PILAR-IZQUIERDO ◽  
NATIVIDAD ORTEGA ◽  
MANUEL PEREZ-MATEOS ◽  
MARÍA D. BUSTO
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Soil Phosphorus ◽  
Film Coating ◽  
P Uptake ◽  
Culture Conditions ◽  
Inorganic P ◽  
Plant Utilization ◽  
Coating Formation ◽  
Layer Coating

SUMMARYCoating barley seeds with free and immobilized alkaline phosphatase was investigated as a potential means to enhance plant utilization of accumulated soil phosphorus (P). Two coating techniques were studied: film-coating and pelleting. The highest phosphatase activity retention in the coating layer, ranging from 0·48 to 0·67, was observed when seeds were film-coated with phosphatase–polyresorcinol complex (PPC). The germination of seeds film-coated or pelleted with alkaline phosphatase ranged from 0·84 to 0·97 or 0·14 to 0·25, respectively. Low germination of the pelleted seeds was attributed to freezing the seeds in liquid nitrogen (N) for the layer coating formation. Pelleted seeds were not used in the remainder of the studies. Under pot culture conditions, an increase in the soil inorganic P was detected when the seeds were film-coated with phosphatase. Moreover, the film-coating significantly increased the P uptake by plants (between 25 and 31% after 35 days after planting (DAP)). The present study showed that the seed film-coating with free and immobilized phosphatase increased the phosphatase activity in the rhizosphere and the P uptake by plants.

scholarly journals Effects of Cowpea-Amaranth Intercropping and Fertiliser Application on Soil Phosphatase Activities, Available Soil Phosphorus, and Crop Growth Response

Agronomy ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 79 ◽  
Author(s):  
Buhlebelive Mndzebele ◽  
Bhekumthetho Ncube ◽  
Melake Fessehazion ◽  
Tafadzwanashe Mabhaudhi ◽  
Stephen Amoo ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Crop Production ◽  
Soil Phosphorus ◽  
Phosphorus Concentration ◽  
Acid And Alkaline Phosphatase ◽  
Available Soil Phosphorus ◽  
Fertiliser Application ◽  
Inorganic Fertilisers

Low available soil phosphorus (P) is associated with its immobility, which renders it unavailable for plant uptake. In addition, farmers normally apply inorganic fertilisers to legumes to activate soil-bound phosphorus using root exudates. Sufficient soil mineral nutrition is key to sustainable crop production, and hence food and nutritional security. The aim of this study was to quantify the acid and alkaline phosphatase activity as an indicator of P supply and availability under varying levels of nitrogen, phosphorus and potassium (NPK) fertilization and different cropping systems. An intercropping (cowpea and amaranth) and fertiliser (control, 25%, 50%, and 100% of the recommended NPK levels) field trial was laid out in a 2 × 4 factorial treatment structure in a completely randomized design (CRD) with four replications. There was higher acid and alkaline phosphatase activity in the rhizosphere of cowpea and amaranth grown as sole crops compared to those from intercropping. The cowpea and amaranth plants grown without fertiliser or 25% NPK had the highest rhizospheric phosphatase activity, while 100% NPK application exhibited the least. The markedly higher phosphatase activity from the low fertiliser application treatments indicates the possible stimulation of microbial activity to supplement P demands for the crops. The study revealed that the application of lower rates inorganic fertilisers in a legume intercrop stimulates the activity of the phosphatase enzymes, which can subsequently liberate soil-bound phosphorus. Plant tissue phosphorus concentration of cowpea and amaranth plants increased proportionately to the increase in fertiliser application up to 50% of the recommended NPK level. The land equivalent ratio (LER) was greater than 1, indicating that it is more beneficial to intercrop cowpea and amaranth as opposed to growing them as sole crops. Overall, the application of NPK fertilizer to amounts of up to 50%, based on the results of this study, appear to be better than 100% in terms of biomass accumulation and phosphate activity.

scholarly journals The effect of combination of indigenous phosphate solubilizing bacteria of Riau, Indonesia on the available phosphorus and phosphorus uptake of soybean

2018 ◽  
Vol 10 (3) ◽  
pp. 146-150
Author(s):  
LUFITANUR ALFIAH ◽  
DELITA ZUL ◽  
NELVIA NELVIA
Keyword(s):  
Phosphatase Activity ◽  
Soil Phosphorus ◽  
Peat Soil ◽  
Phosphorus Uptake ◽  
P Uptake ◽  
Available P ◽  
Available Phosphorus ◽  
Alkaline Soil ◽  
Phosphate Solubilizing Bacteria ◽  
Phosphate Solubilizing

Alfiah L, Zul D, Nelvia N. 2018. The effect of combination of indigenous phosphate solubilizing bacteria of Riau, Indonesia on the available phosphorus and phosphorus uptake of soybean. Nusantara Bioscience 10: 146-150. Despite the abundant amount of phosphorus (P) in the soil, P uptake by the plants is very limited. In acidic soil, phosphorus (P) is bound to aluminum (Al) and iron (Fe), whereas in the alkaline soil, phosphorus (P) is bound to calcium (Ca). The improvement of efficiency and availability of P to plants can be made by utilizing a group of solubilizing phosphate microorganisms. Potency test to investigate the P solubility by Phosphate Solubilizing Bacteria (PSB) has been conducted by isolating the bacteria from peat soil at Biosphere Reserves of Giam Siak Kecil Bukit Batu, Riau, Indonesia. The semi-quantitative test revealed that the PSB were able to dissolve Ca3 (PO4)2, FePo4 and phosphate rock. However, the adaptation ability and potency of PSB from indigenous Riau peat soil inoculated into soybean (Glycine max L. Merr) plants in the mineral soil have not yet been investigated. The present study was carried out from March to June 2015 on the alluvial soil in Babussalam Village, Rambah Sub-district, Rokan Hulu District, Riau. The aim of this study was to determine the effect of PSB inoculation on bacterial population and phosphatase activity. The study also aimed to determine the available P and P uptake and their impact on soybean growth and production. The study employed a factorial experiment laid out in a completely randomized design (CRD) consisted of two factors, i.e., soil treatment and PSB. The first factor comprised of two levels, i.e., T0: non-sterilized soil, T1: sterilized soil. The second factor consisted of 4 levels, i.e., B0: without PSB inoculation, B1: inoculation using 2 isolates of PSB (BB_UB6 and BB_K9), B2: inoculation using 3 isolates of PSB (BB_UB6, BB_K9 and BB_K2), and B3: inoculation using 4 isolates of PSB (BB_UB6, BB_K9, BB_K2, and BB_HS13). The results showed that inoculation of starter 3 had the highest phosphatase activity rate of 12.43 μg p NP g-1 hour-1. The highest available P was produced by starter 2, while the P uptake on non-sterilized soil was higher than that on the sterilized soil at 2.63 mg plant-1. PSB inoculation and soil sterilization did not significantly affect the population of phosphate solubilizing bacteria.

Phosphatase activities and phosphorus uptake from inositol phosphate by ectomycorrhizal fungi

1992 ◽  
Vol 70 (4) ◽  
pp. 794-801 ◽  
Author(s):  
Robert K. Antibus ◽  
Robert L. Sinsabaugh ◽  
Arthur E. Linkins
Keyword(s):  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Ectomycorrhizal Fungi ◽  
Inositol Phosphate ◽  
P Uptake ◽  
Inositol Hexaphosphate ◽  
Inorganic P ◽  
Isozyme Patterns ◽  
Cell Extracts ◽  
Nitrophenyl Phosphate

To better understand the physiological importance of acid phosphatase activity we examined the effects of inorganic and organic phosphorus growth sources on enzyme activity and 32P uptake in several ectomycorrhizal fungi. Mycelium of eight isolates from four basidiomycete species demonstrated optimal p-nitrophenyl phosphatase activity at pH 4.5 or 5.0. Acid phosphatase activities varied between strains of Scleroderma citrinum and between the species examined. Interspecific differences in isozyme patterns of whole cell extracts were apparent in native polyacrylamide gels. The isoelectric points of the predominant phosphatases in whole cell extracts were in the pH 5.0 to 5.5 range. Growth of fungi on inositol hexaphosphate versus inorganic P did not affect the isozyme patterns detected by either electrophoretic method. Growth on inositol hexaphosphate affected surface and soluble activities towards p-nitrophenyl phosphate and inositol phosphate to different degrees in species examined. Phytase activity was sufficient to produce a net release of P in all isolates. Growth on inositol hexaphosphate was associated with increased uptake of 32P from inositol polyphosphate in four of five species examined. Acid phosphatase, measured with p-nitrophenyl phosphate, was positively correlated with 32P uptake. Decreased phytase activities measured for inositol hexaphosphate grown mycelium were associated with increased P influx in such mycelium. Both phosphatase activity and 32P uptake were subject to inorganic P inhibition with 32P uptake demonstrating a greater sensitivity. These results provide further evidence for the role of surface acid phosphatases in organic P utilization by ectomycorrhizal fungi. Key words: acid phosphatase, ectomycorrhizal fungi, phytase.

scholarly journals Comparison of fractionation methods for soil phosphorus with soils subjected to various long-term fertilization regimes on a calcareous soil

2021 ◽  
Vol 3 ◽  
pp. e3
Author(s):  
Xin Jin ◽  
Changlu Hu ◽  
Asif Khan ◽  
Shulan Zhang ◽  
Xueyun Yang ◽  
...  
Keyword(s):  
Calcareous Soil ◽  
Soil Phosphorus ◽  
Calcareous Soils ◽  
P Uptake ◽  
Organic P ◽  
P Fractions ◽  
P Fractionation ◽  
Inorganic P ◽  
Soil P

Background Diverse phosphorus (P) fractionation procedures presented varying soil P fractions, which directly affected P contents and forms, and their biological availability. Purpose To facilitate the selection of phosphorus (P) fractionation techniques, we compared two procedures based on a long-term experiment on a calcareous soil. Methods The soils containing a gradient P levels were sampled from seven treatments predictor under various long-term fertilizations. The P fractions were then separated independently with both fractionation procedures modified by Tiessen-Moir and Jiang-Gu. Results The results showed that the labile P in Jiang-Gu is significantly lower than that in Tiessen-Moir. The iron and aluminium-bounded P were greater in Jiang-Gu by a maximum of 46 mg kg−1 than Tiessen-Moir. Jiang-Gu fractionation gave similar Ca bounded P to that Tiessen-Moir did at low P level but greater contents at high P level. The two methods extracted much comparable total inorganic P. However, Tiessen-Moir method accounted less total organic P than ignition or Jiang-Gu method (the organic P (Po) estimated by subtract the total inorganic P (Pi) in Jiang-Gu fractionation from the total). P uptake by winter wheat was significantly and positively correlated with all phosphorus fractions in Jiang-Gu; Resin-P, NaHCO3-Pi, D. HCl-P, C. HCl-Pi, NaOH-Po, total-Po in Tiessen-Moir; P fraction categories of Ca-P, Fe & Al-P and total-Pi in both fractionations. Path coefficients indicated that Ca2-P in Jiang-Gu, NaHCO3-Pi and D. HCl-P in Tiessen-Moir had the higher and more significant direct contributions to P uptake among P fractions measured. Conclusions Our results suggested that Jiang-Gu procedure is a better predictor in soil P fractionation in calcareous soils, although it gives no results on organic P fractions.

scholarly journals Role of Organic Anions and Phosphatase Enzymes in Phosphorus Acquisition in the Rhizospheres of Legumes and Grasses Grown in a Low Phosphorus Pasture Soil

Plants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1185 ◽  
Author(s):  
Driss Touhami ◽  
Richard W. McDowell ◽  
Leo M. Condron
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Plant Species ◽  
Alkaline Phosphatase Activity ◽  
White Clover ◽  
Organic Anions ◽  
Organic P ◽  
Inorganic P ◽  
Pasture Soil ◽  
Rhizosphere Processes

Rhizosphere processes play a critical role in phosphorus (P) acquisition by plants and microbes, especially under P-limited conditions. Here, we investigated the impacts of nutrient addition and plant species on plant growth, rhizosphere processes, and soil P dynamics. In a glasshouse experiment, blue lupin (Lupinus angustifolius), white clover (Trifolium repens L.), perennial ryegrass (Lolium perenne L.), and wheat (Triticum aestivum L.) were grown in a low-P pasture soil for 8 weeks with and without the single and combined addition of P (33 mg kg−1) and nitrogen (200 mg kg−1). Phosphorus addition increased plant biomass and total P content across plant species, as well as microbial biomass P in white clover and ryegrass. Alkaline phosphatase activity was higher for blue lupin. Legumes showed higher concentrations of organic anions compared to grasses. After P addition, the concentrations of organic anions increased by 11-,10-, 5-, and 2-fold in the rhizospheres of blue lupin, white clover, wheat, and ryegrass, respectively. Despite the differences in their chemical availability (as assessed by P fractionation), moderately labile inorganic P and stable organic P were the most depleted fractions by the four plant species. Inorganic P fractions were depleted similarly between the four plant species, while blue lupin exhibited a strong depletion of stable organic P. Our findings suggest that organic anions were not related to the acquisition of inorganic P for legumes and grasses. At the same time, alkaline phosphatase activity was associated with the mobilization of stable organic P for blue lupin.

LEUCOCYTE METABOLISM IN PREGNANCY

1960 ◽  
Vol XXXV (IV) ◽  
pp. 575-584 ◽  
Author(s):  
C. Borel ◽  
J. Frei ◽  
A. Vannotti
Keyword(s):  
Alkaline Phosphatase ◽  
Oxygen Consumption ◽  
Pregnant Women ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Lactate Production ◽  
Glucose Consumption ◽  
In Pregnancy

ABSTRACT Enzymatic studies, on leucocytes of pregnant women, show an increase of the alkaline phosphatase activity and a decrease of the glucose consumption and lactate production, as well as of proteolysis. The oxygen consumption, with succinate as substrate, does not vary.

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