The effect of cultivar on the changes in protein quality during wilting and ensiling of red clover (Trifolium pratense L.)

2012 ◽  
Vol 151 (4) ◽  
pp. 506-518 ◽  
Author(s):  
M. KRAWUTSCHKE ◽  
N. WEIHER ◽  
J. THAYSEN ◽  
R. LOGES ◽  
F. TAUBE ◽  
...  
Keyword(s):  
White Clover ◽  
Crude Protein ◽  
Trifolium Pratense ◽  
Protein Quality ◽  
Red Clover ◽  
Field Studies ◽  
Protein Nitrogen ◽  
Trifolium Pratense L ◽  
Rumen Undegradable Protein ◽  
True Protein

SUMMARYRed clover (Trifolium pratense L.) silage usually contains lower contents of non-protein nitrogen (NPN) compared with other forage legumes. This is often attributed to the polyphenol oxidase (PPO) activity in red clover, although in most field studies the PPO activity was not measured. Therefore, a laboratory ensiling experiment with three red clover cultivars and one white clover cultivar as control grown in two management systems (with and without mechanical stress) over 2 consecutive years was conducted. Fresh, wilted and ensiled clover herbage was sampled at four cutting dates per year to determine the crude protein (CP) fractions according to the Cornell Net Carbohydrate and Protein System. The specific PPO activity was measured photometrically in fresh clover leaves. The content of CP fraction A (NPN) increased from fresh over wilted to ensiled clover herbage at the expense of the content of CP fraction B (true protein), irrespective of species, cultivar and year. The most important source of variation for all CP fractions and the calculated rumen-undegradable protein contents was generally the herbage condition, except for CP fraction C (unavailable protein). White clover silage consisted of higher contents of CP fraction A and lower contents of CP fraction B3 in CP compared with red clover silage. As a result, the calculated rumen-undegradable protein content of white clover silage was lower than that of all red clover cultivars. In conclusion, the extent of proteolysis during ensiling among the silages made from the herbage of different red clover cultivars was primarily influenced by the stage of maturity at harvesting and the degree of wilting at ensiling. The variation in specific PPO activity could not be related to the extent of proteolysis.

scholarly journals Estimation of red clover (Trifolium pratense L.) forage quality parameters depending on the stage of growth

2011 ◽  
Vol 27 (4) ◽  
pp. 1563-1569
Author(s):  
J. Markovic ◽  
R. Strbanovic ◽  
D. Terzic ◽  
R. Stanisavljevic ◽  
D. Djokic ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Trifolium Pratense ◽  
Red Clover ◽  
Neutral Detergent Fiber ◽  
Net Energy ◽  
The Third ◽  
Stages Of Growth ◽  
Feed Value ◽  
Trifolium Pratense L

The objective of this study was to investigate the changes that take place in nutrient values of red clover (Trifolium pratense L.) cv K-27 at different stages of growth in the second and the third cut. The samples from different stages of growth: mid-bud stage, around 60% flowering and full flowering were investigated for crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), digestible dry matter (DDM), dry matter intake (DMI), relative feed value (RFV), total digestible nutrients (TDN), net energy-lactation (NEl), net energy-maintenance (NEm) and net energy-gain (NEg) content. Higher content of crude protein was found in the third cut (262.1 g kg-1 of DM) than in the second cut (260.8 g kg-1 of DM) in the first stage of development. TDN, DDM, DMI, RFV, NEl, NEm and NEg were calculated according to the appropriate equations adapted from common formulas for forages. The stage of plant development has a significant influence on the chemical composition and the relative feed value of red clover. The achieved results show that adequate maturity stage for cutting might be at mid-bloom stage, when crude protein content, NEl, NEm and NEg content are high.

A COMPARISON OF PROTEIN DEGRADATION DURING WILTING AND ENSILING OF SIX FORAGE SPECIES

1983 ◽  
Vol 63 (4) ◽  
pp. 903-912 ◽  
Author(s):  
Y. A. PAPADOPOULOS ◽  
B. D. McKERSIE
Keyword(s):  
Trifolium Pratense ◽  
Proteolytic Enzymes ◽  
Red Clover ◽  
Phleum Pratense ◽  
Protein Hydrolysis ◽  
Protein Nitrogen ◽  
Forage Species ◽  
Trifolium Pratense L ◽  
Medicago Sativa L ◽  
Dactylis Glomerata L

Protein hydrolysis was examined during wilting and ensiling of first and second cut herbage of alfalfa (Medicago sativa L.), red clover (Trifolium pratense L.), birdsfoot trefoil (Lotus corniculatus L.), orchardgrass (Dactylis glomerata L.), bromegrass (Bromus inermis Leyss.) and timothy (Phleum pratense L.). During a 24-h wilting period, protein was hydrolyzed to soluble non-protein nitrogen (SNPN) more extensively in first cut than in second cut herbage. In both cuts the amount of protein hydrolysis occurring during wilting was greatest in alfalfa and least in red clover. After 30 days ensiling at 30 °C, SNPN content, expressed as percent of total nitrogen, was influenced by forage species, dry matter (DM) content, and cut. The extent of protein hydrolysis during ensiling was highest in alfalfa and lowest in red clover. In first cut silages, protein hydrolysis increased with DM content, but in the second cut silages, protein hydrolysis decreased as DM content increased. The amount of protein hydrolyzed during wilting or ensiling was not correlated with plant proteinase activity measured using azocasein as substrate. Consequently, the properties of the plant proteolytic enzymes associated with each species as well as the management of the forage prior to ensiling appear to influence the extent of proteolysis.Key words: Proteolysis, silage, haylage, proteinase

scholarly journals GROWTH AND DEVELOPMENT OF LEGUME AND GRASS COMPONENTS IN MIXED GRASSLANDS GROWN IN THE CENTRAL BALKAN MOUNTAIN

2019 ◽  
Vol 17 (1) ◽  
pp. 19-27
Author(s):  
T. Bozhanska ◽  
B. Churkova
Keyword(s):  
White Clover ◽  
Growth And Development ◽  
Trifolium Pratense ◽  
Red Clover ◽  
Kentucky Bluegrass ◽  
Meadow Fescue ◽  
Average Growth ◽  
Timothy Grass ◽  
Trifolium Pratense L ◽  
Mixed Grasslands

PURPOSE of the present study is to establish the growth, development and competitiveness of grass and legume components in mixed grasslands grown under the conditions of the Central Balkan Mountains. METHODS: In the spring of 2014-2016, the growth and development of typical meadow legumes and grass species for that region were observed in the experimental field of RIMSA - Troyan, in double mixtures: 1. Bird's-foot-trefoil - Red fescue; 2. White clover - Perennial ryegrass; 3. White clover - Kentucky bluegrass; 4. Red clover - Timothy-grass; 5. Blue hybrid alfalfa - Cock's foot; 6. Red clover - Meadow fescue. RESULTS: From all grass crops - Dactylis glomerata L. has the most pronounced competitive ability with respect to the biometric height indicator of plants, while Poa pratensis L. has the slightest one. For the three-year study period, Trifolium repens L. in its mixture with Lolium perenne L. recorded the lowest average growth values compared to the other legumes included in the experiment. CONCLUSIONS: The best intercompatibility and, respectively, the most favorable combination of grass and legume components is found between Trifolium pratense L. and Festuca pratensis L., and the weakest among the components in the blue hybrid alfalfa - cock's foot mixture. The length of the flower-bearing stems and leaf stalks of Trifolium pratense L. in the mixed grassland with meadow fescue have higher values (41.8:89.0 cm) and those with timothy-grass are lower (38.1:52.5 cm).

EFFECT OF THE ROOT-LESION NEMATODE ON YIELD OF FOUR FORAGE LEGUMES UNDER GREENHOUSE CONDITIONS

1969 ◽  
Vol 49 (4) ◽  
pp. 505-509 ◽  
Author(s):  
C. B. Willis ◽  
L. S. Thompson
Keyword(s):  
White Clover ◽  
Trifolium Pratense ◽  
Red Clover ◽  
Birdsfoot Trefoil ◽  
Pratylenchus Penetrans ◽  
Lesion Nematodes ◽  
Trifolium Pratense L ◽  
Significant Yield ◽  
Medicago Sativa L ◽  
Root Lesion Nematodes

Foliage yields of red clover (Trifolium pratense L.), alfalfa (Medicago sativa L.), white clover (Trifolium repens L.), and birdsfoot trefoil (Lotus corniculatus L.) were reduced by root-lesion nematodes [Pratylenchus penetrans (Cobb, 1917) Filip. and Stekh, 1941]. Foliage yields generally decreased with increased infestation levels. Significant yield reductions were recorded at the time of first cutting for white clover and birdsfoot trefoil, but not until several cuts later for red clover and alfalfa. Mean yields of birdsfoot trefoil, red clover, alfalfa, and white clover infested with 7,500 nematodes per pail were 50, 73, 83, and 93%, respectively, of control yields.

Protein degradation and ensiling characteristics of red clover and alfalfa wilted under varying levels of shade

1999 ◽  
Vol 79 (2) ◽  
pp. 209-222 ◽  
Author(s):  
V. N. Owens ◽  
K. A. Albrecht ◽  
R. E. Muck
Keyword(s):  
Protein Degradation ◽  
Dry Matter ◽  
Trifolium Pratense ◽  
Red Clover ◽  
Nonprotein Nitrogen ◽  
Protein Nitrogen ◽  
Harvest Dates ◽  
Trifolium Pratense L ◽  
Medicago Sativa L ◽  
Time Required

The conversion of protein nitrogen (PN) to nonprotein nitrogen (NPN) in forages occurs rapidly and extensively during wilting and ensiling. The objectives of this study were to determine whether the amount of time between cutting and ensiling affects protein degradation in red clover (Trifolium pratense L.) and alfalfa (Medicago sativa L.) silage and to document pre- and post-ensiling characteristics of these two species. Forage from the second (24 August 1993), first (27 May 1994), and second (10 July 1995) growth cycles was harvested with hand clippers to a 5-cm stubble height between 09:00 and 10:00 h on each of the three harvest dates. Herbage was allowed to wilt to a targeted dry matter (DM) content of 350 g kg−1 under 0, 30, 73, and 100% shade (wilting treatment) and ensiled in 100-mL polypropylene centrifuge tubes. Time required to reach the desired DM varied each year, with the greatest range in drying times occurring in 1993. Starch was lower and sugar higher in fresh and wilted red clover than in alfalfa. Fresh red clover contained less NPN than alfalfa in 1993 and 1995 (P < 0.05) and similar amounts in 1994 (P > 0.05). Wilting treatment had no effect on NPN in wilted forage in 1994 and 1995, a result of nearly equal wilting times across shade levels. In 1993, however, NPN increased and starch decreased with shade. Within species, further protein hydrolysis during ensiling resulted in similar NPN levels in silage from all wilting treatments in 1993, despite differences in NPN in pre-ensiled wilted forage. Red clover silage consistently contained less NPN than alfalfa. Wilting treatments did not consistently affect sugar and starch concentrations of silage from either species, although there was a tendency for sugar and starch to decrease with longer wilting periods. Key words: alfalfa, red clover, protein degradation, ensiling

The effects of white clover mosaic virus infection on the yield of red clover (Trifolium pratense L.) in mixtures and in pure stands

1982 ◽  
Vol 98 (2) ◽  
pp. 455-460 ◽  
Author(s):  
S. W. Scott
Keyword(s):  
Virus Infection ◽  
Mosaic Virus ◽  
White Clover ◽  
Trifolium Pratense ◽  
Red Clover ◽  
Trifolium Pratense L ◽  
White Clover Mosaic Virus ◽  
Different Levels ◽  
Negative Linear Relationship ◽  
Mosaic Virus Infection

SUMMARYThe effects of white clover mosaic virus infection on the yield of two red clover varieties grown alone and in mixtures with either perennial or hybrid ryegrasses were examined. The spread of this virus within the pure clover plots was rapid. Clover drymatter yields were reduced and these losses were greater in the grass–clover mixtures than in the pure clover plots. Reductions in yield of about 70% were recorded at the first cut in mixtures of Sabtoron and Sabrina. The response of the clover to different levels of virus infection was best described by a negative linear relationship.Yields of Sabrina hybrid ryegrass increased as the levels of virus infection in the clover increased. This compensated for the losses in yield of the legume, leaving total sward yields unaffected by the virus.

scholarly journals Selection by Lambs Grazing Plantain (Plantago lanceolata L.), Chicory (Cichorium intybus L.), White Clover (Trifolium repens L.), Red Clover (Trifolium pratense L.) and Perennial Ryegrass (Lolium perenne L.) across Seasons

Animals ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2292
Author(s):  
Sharini C. Somasiri ◽  
Paul R. Kenyon ◽  
Patrick C.H. Morel ◽  
Stephen T. Morris ◽  
Peter D. Kemp
Keyword(s):  
Relative Abundance ◽  
Perennial Ryegrass ◽  
White Clover ◽  
Trifolium Pratense ◽  
Block Design ◽  
Plantago Lanceolata ◽  
Live Weight ◽  
Red Clover ◽  
Cichorium Intybus ◽  
Trifolium Pratense L

Plantain (Plantago lanceolata) and chicory (Cichorium intybus) are now widely used in combination with clover species to provide greater annual lamb live weight gains than perennial ryegrass and white clover pasture. Reported selective grazing of the species in herb-clover mixes could potentially detrimentally change the relative abundance of species and decrease lamb production. Lambs were offered three herbage treatments: Pasture (perennial ryegrass and white clover) mix, plantain (plantain, red clover and white clover) mix and a chicory (chicory, plantain, red clover and white clover) mix in each of four seasons for two years. The experiment was a randomized complete block design with three replicates with 18–30 lambs per treatment replicate depending on the season. Lambs were rotationally grazed and fed ad libitum. Selection by the lambs of individually tagged plants within the pasture treatments was observed for three days on two occasions per season. Red clover was the most selected species on day 1, but by day 3 there was no difference in the selection of the species (p < 0.05). Plantain and chicory in the plantain and chicory mixes were selected less on day 1 in autumn relative to the other seasons (p < 0.05). It was concluded that three days of grazing before moving lambs maintained the relative abundance of species in the herb-clover mixes.

scholarly journals Yield and forage quality of red clover (Trifolium pratense L.) cultivars in the lowland and the mountain regions

2011 ◽  
Vol 50 (No. 9) ◽  
pp. 391-396 ◽  
Author(s):  
J. Leto ◽  
M. Knežević ◽  
K. Bošnjak ◽  
D. Maćešić ◽  
Z. Štafa ◽  
...  
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Trifolium Pratense ◽  
Red Clover ◽  
Mountain Region ◽  
Crude Fiber ◽  
Stem Height ◽  
Green Mass ◽  
Growing Seasons ◽  
Trifolium Pratense L

Six Trifolium pratense L. cultivars, five diploid and one tetraploid, were grown in the lowland region (123 m altitude) and the mountain region (650 m altitude). Dry matter (d.m.) and green mass (g.m.) yield, stem height and leaf proportion were assessed from each of the three growing seasons. Chemical composition was assessed from the average samples of all cuts in the second year of the experiment. Greater green mass (54.14 t/ha) and dry matter yield (9.86&nbsp;t/ha), stem height (0.61 m), crude protein (157.6 g/kg), crude fiber (222.9 g/kg), crude ash (100.68 g/kg) and crude fat (30.09 g/kg) content were observed in the mountain region, compared to the lowland region (45.61 t/ha g.m. 8.92 t/ha d.m. 0.59 stem height, 156.38 g/kg crude protein, 216.6 g/kg crude fiber, 94.85 g/kg crude ash, 24.98 g/kg crude fat). The greater leaf proportion (47.2%) and nitrogen free extract content (420.13 g/kg) were observed in the lowland region compared to the mountain region (42.95% and 402.99 g/kg, respectively). A significant location &times; cultivar interaction was found for all investigated parameters except for leaf proportion.&nbsp;

Sign in / Sign up

Export Citation Format

Share Document