Immunodiagnosis of human dirofilariasis by enzyme-linked immunosorbent assay using recombinant DNA-derived fusion protein
1992 ◽
Vol 66
(3)
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pp. 220-226
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Keyword(s):
ABSTRACTAn enzyme-linked immunosorbent assay (ELISA) using antigenic β-galactosidase-Dirofilaria immitis recombinant fusion protein (FP) obtained by the recombinant DNA technique provided a useful diagnostic tool for human dirofilariasis. D. immitis-infected human sera reacted strongly with FP that was immobilized with anti-β-galactosidase monoclonal antibody on microplates. However, the FP did not react with sera from patients with other filariasis. In detection of anti-D. immitis IgG antibody. ELISA using FP was highly sensitive and specific compared to that using crude somatic antigen.
1986 ◽
Vol 60
(3)
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pp. 239-243
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1992 ◽
Vol 37
(3)
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pp. 259-273
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2003 ◽
Vol 10
(6)
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pp. 1136-1140
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Keyword(s):
1988 ◽
Vol 101
(2)
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pp. 405-410
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2002 ◽
Vol 109
(1-2)
◽
pp. 45-58
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Keyword(s):
1986 ◽
Vol 21
(3)
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pp. 165-171
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