scholarly journals Enzyme-linked immunosorbent assay for streptococcal M protein antibodies

1976 ◽  
Vol 3 (5) ◽  
pp. 501-505
Author(s):  
H Russell ◽  
R R Facklam ◽  
L R Edwards
Keyword(s):  
Immunosorbent Assay ◽  
Cell Walls ◽  
M Protein ◽  
Enzyme Linked Immunosorbent Assay ◽  
Preliminary Results ◽  
Streptococcal M Protein ◽  
Highly Sensitive ◽  
Bactericidal Antibody ◽  
Human Sera ◽  
M 12

The enzyme-linked immunosorbent assay (ELISA) described by Engvall and Perlmann, which uses antigen-coated tubes and enzyme-labeled anti-immunoglobulin, has been used for the detection of antibodies against streptococcal M protein. The antigen used in the assay was obtained by guanidine extraction of type M-12 streptococcal cell walls followed by hydroxyapatite chromatography. This antigen has the capacity to elicit bactericidal antibodies in rabbits. The results show that the ELISA is specific and highly sensitive for the detection of antibodies in rabbit and human antisera. Preliminary results suggest that, when M-12 antigen is used, the antibodies detected by ELISA are the same antibodies detected in the bactericidal test. The assay has been performed with human and rabbit sera. There was a 96% agreement between bactericidal and ELISA results with rabbit sera and 97.5% agreement with human sera. All bactericidal antibody-positive sera tested thus far yielded positive ELISA results.

Immunodiagnosis of human dirofilariasis by enzyme-linked immunosorbent assay using recombinant DNA-derived fusion protein

1992 ◽  
Vol 66 (3) ◽  
pp. 220-226 ◽  
Author(s):  
S. Sun ◽  
K. Sugane
Keyword(s):  
Fusion Protein ◽  
Immunosorbent Assay ◽  
Dirofilaria Immitis ◽  
Recombinant Dna ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibody ◽  
Highly Sensitive ◽  
Human Sera ◽  
Human Dirofilariasis ◽  
Antibody Elisa

ABSTRACTAn enzyme-linked immunosorbent assay (ELISA) using antigenic β-galactosidase-Dirofilaria immitis recombinant fusion protein (FP) obtained by the recombinant DNA technique provided a useful diagnostic tool for human dirofilariasis. D. immitis-infected human sera reacted strongly with FP that was immobilized with anti-β-galactosidase monoclonal antibody on microplates. However, the FP did not react with sera from patients with other filariasis. In detection of anti-D. immitis IgG antibody. ELISA using FP was highly sensitive and specific compared to that using crude somatic antigen.

scholarly journals Development of a Highly Sensitive and Specific Enzyme-Linked Immunosorbent Assay Based on Recombinant Matrix Protein for Detection of Avian Pneumovirus Antibodies

2000 ◽  
Vol 38 (11) ◽  
pp. 4010-4014 ◽  
Author(s):  
Baldev R. Gulati ◽  
Kjerstin T. Cameron ◽  
Bruce S. Seal ◽  
Sagar M. Goyal ◽  
David A. Halvorson ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Matrix Protein ◽  
Clinical Signs ◽  
M Protein ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Specific Test ◽  
Avian Pneumovirus ◽  
Highly Sensitive ◽  
The Matrix

The matrix (M) protein of avian pneumovirus (APV) was evaluated for its antigenicity and reliability in an enzyme-linked immunosorbent assay (ELISA) for diagnosis of APV infection, a newly emergent disease of turkeys in United States. Sera from APV-infected turkeys consistently contained antibodies to a 30-kDa protein (M protein). An ELISA based on recombinant M protein generated in Escherichia coli was compared with the routine APV ELISA that utilizes inactivated virus as antigen. Of 34 experimentally infected turkeys, 33 (97.1%) were positive by M protein ELISA whereas only 18 (52.9%) were positive by routine APV ELISA 28 days after infection. None of the serum samples from 41 uninfected experimental turkeys were positive by M protein ELISA. Of 184 field sera from turkey flocks suspected of having APV infection, 133 (72.3%) were positive by M protein ELISA whereas only 99 (53.8%) were positive by routine APV ELISA. Twelve serum samples, which were negative by M protein ELISA but positive by routine APV ELISA, were not reactive with either recombinant M protein or denatured purified APV proteins by Western analysis. This indicates that the samples had given false-positive results by routine APV ELISA. The M protein ELISA was over six times more sensitive than virus isolation (11.5%) in detecting infections from samples obtained from birds showing clinical signs of APV infection. Taken together, these results show that ELISA based on recombinant M protein is a highly sensitive and specific test for detecting antibodies to APV.

Development of a Highly Sensitive Enzyme-Linked Immunosorbent Assay Based on Polyclonal Antibodies for the Detection of Polychlorinated Dibenzo-p-dioxins

1998 ◽  
Vol 70 (6) ◽  
pp. 1092-1099 ◽  
Author(s):  
Yukio Sugawara ◽  
Shirley J. Gee ◽  
James R. Sanborn ◽  
S. Douglass Gilman ◽  
Bruce D. Hammock
Keyword(s):  
Immunosorbent Assay ◽  
Polyclonal Antibodies ◽  
Enzyme Linked Immunosorbent Assay ◽  
Highly Sensitive

scholarly journals Evaluation of Serum Bactericidal Antibody Assays forHaemophilus influenzaeSerotype a

2010 ◽  
Vol 18 (2) ◽  
pp. 243-247 ◽  
Author(s):  
Nadine G. Rouphael ◽  
Sarah Satola ◽  
Monica M. Farley ◽  
Karen Rudolph ◽  
Daniel S. Schmidt ◽  
...  
Keyword(s):  
Haemophilus Influenzae ◽  
American Indian ◽  
Immunosorbent Assay ◽  
Bactericidal Activity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Alaskan Native ◽  
Serum Bactericidal Activity ◽  
Aboriginal Populations ◽  
Antibody Assays ◽  
Bactericidal Antibody

ABSTRACTHaemophilus influenzaetype a (Hia) is an important pathogen for some American Indian, Alaskan native, and Northern Canada aboriginal populations. Assays to measure serum bactericidal activity (SBA) to Hia have not been developed or validated. Here, we describe two methods for the measurement of SBA: SBA with a viability endpoint (CFU counts) and SBA with a fluorometric endpoint using alamarBlue as the metabolic indicator. Both SBA assays measure Hia-specific functional antibody and correlate with anti-Hia IgG enzyme-linked immunosorbent assay (ELISA) concentration of naturally acquired antibodies.

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