Serological Investigations with Loa loa Antigens

1972 ◽  
Vol 46 (3) ◽  
pp. 241-250 ◽  
Author(s):  
E. O. Ogunba
Keyword(s):  
Sensitivity And Specificity ◽  
Fluorescent Antibody ◽  
Broad Band ◽  
Antibody Test ◽  
Double Diffusion ◽  
Diagnostic Value ◽  
Loa Loa ◽  
Diffusion Test ◽  
Agar Gel ◽  
Antibody Tests

Antigens prepared from adult worms and microfilariae of Loa loa were used for an assessment of the sensitivity and specificity in diagnosis of double immuno-diffusion in agar gel, haemagglutination and fluorescent antibody tests.Both antigens formed a broad band which moved slowly towards the cathode on electrophoresis, and each antigen separated into two protein fractions when passed through Sephadex G-200.Both precipitating and non-precipitating antibodies were detected in sera from Loa loa patients. The haemagglutination test and fluorescent antibody test were sensitive in detecting Loa loa antibodies and would be of diagnostic value, but the double diffusion test in agar-gel was insensitive and would have a limited value in loiasis diagnosis.

scholarly journals Evaluation of Six Commercial Point-of-Care Tests for Diagnosis of Acute Dengue Infections: the Need for Combining NS1 Antigen and IgM/IgG Antibody Detection To Achieve Acceptable Levels of Accuracy

2011 ◽  
Vol 18 (12) ◽  
pp. 2095-2101 ◽  
Author(s):  
Stuart D. Blacksell ◽  
Richard G. Jarman ◽  
Mark S. Bailey ◽  
Ampai Tanganuchitcharnchai ◽  
Kemajittra Jenjaroen ◽  
...  
Keyword(s):  
Dengue Fever ◽  
Sensitivity And Specificity ◽  
Dengue Infection ◽  
Antibody Test ◽  
Antibody Detection ◽  
Sample Collection ◽  
Igg Antibody ◽  
Igm Antibody ◽  
Ns1 Antigen ◽  
Antibody Tests

ABSTRACTSix assays were evaluated in this study to determine their suitability for the diagnosis of acute dengue infection using samples from 259 Sri Lankan patients with acute fevers (99 confirmed dengue cases and 160 patients with other confirmed acute febrile illnesses): (i) the Merlin dengue fever IgG & IgM combo device (Merlin), (ii) the Standard Diagnostics Dengue Duo nonstructural 1 (NS1) antigen and IgG/IgM combo device (Standard Diagnostics, South Korea), (iii) the Biosynex Immunoquick dengue fever IgG and IgM (Biosynex, France) assay, (iv) the Bio-Rad NS1 antigen strip (Bio-Rad, France), (v) the Panbio Dengue Duo IgG/IgM Cassette (Inverness, Australia), and (vi) the Panbio dengue NS1 antigen strip (Inverness, Australia). The median number of days of fever prior to admission sample collection was 5 days (interquartile range, 3 to 7 days). Sensitivity and specificity of the NS1 antigen tests ranged from 49 to 59% and from 93 to 99%, respectively, and sensitivity and sensitivity of the IgM antibody test ranged from 71 to 80% and from 46 to 90%, respectively. Combining the NS1 antigen and IgM antibody results from the Standard Diagnostics Dengue Duo test gave the best compromise of sensitivity and specificity (93% and 89%, respectively) and provided the best sensitivity in patients presenting at different times after fever onset. The Merlin IgM/IgG antibody tests correctly classified 64% and 86% of the primary and secondary dengue infection cases, respectively, and the Standard Diagnostics IgM/IgG antibody tests correctly classified 71% and 83% of the primary and secondary dengue infection cases, respectively. This study provides strong evidence of the value of combining dengue antigen- and antibody-based test results in the rapid diagnostic test (RDT) format for the acute diagnosis of dengue.

scholarly journals Clinical validation of a point-of-care antibody test for COVID-19

2020 ◽  
Author(s):  
Monila Patel ◽  
Yogesh Lakhotia ◽  
Sneha Shah ◽  
Nilay Suthar ◽  
Cherry Shah ◽  
...  
Keyword(s):  
Confidence Interval ◽  
Sensitivity And Specificity ◽  
Significant Role ◽  
Point Of Care ◽  
Antibody Test ◽  
Lateral Flow ◽  
Clinical Validation ◽  
Rt Pcr ◽  
Antibody Tests ◽  
Spike Proteins

AbstractThe objective of this study was to evaluate the performance of a lateral flow antibody test for COVID-19, approved for use in India. Although many point-of-care antibody tests are available globally, they have been subjected to limited clinical validation. This has led to suboptimal outcomes in the field, where antibody tests play a significant role in tracking the immunity of individuals and communities. In this study an antibody test, ImmunoQuick that recognizes antibodies to the Nucleocapsid and Spike proteins of SARS CoV-2 was tested in 100 symptomatic patients with a positive or negative diagnosis of COVID-19, based on RT-PCR results. The overall sensitivity of the test was found to be 86.1% (95% CI: 76.4% to 92.8%) and specificity 100% (95% confidence interval: 73.5% to 100%). The sensitivity reached a peak of 95.7% with samples taken 17 days after the onset of symptoms. Overall, the sensitivity and specificity of the test are sufficient for assessing seroprevalence.

scholarly journals Antigenic similarity of toxins produced by Clostridium botulinum type C and D strains

1980 ◽  
Vol 30 (3) ◽  
pp. 656-660
Author(s):  
K Oguma ◽  
B Syuto ◽  
H Iida ◽  
S Kubo
Keyword(s):  
Heavy Chain ◽  
Clostridium Botulinum ◽  
Double Diffusion ◽  
Diffusion Test ◽  
Agar Gel ◽  
Chain Component ◽  
Type D ◽  
Botulinum Type ◽  
Type C ◽  
Cross Neutralization

Antisera against purified type C1 toxin of Clostridium botulinum and its heavy-chain component cross-neutralized type D toxin. Antisera against partially purified type D toxin cross-neutralized type C1 toxin. From the latter serum, a component which neutralized only type D toxin and a component which equally neutralized both C1 and D toxins were obtained. We concluded that the cross-neutralization was not due to the fact that type C and D strains produce both C1 and D toxins but rather to the fact that the toxins have an antigen(s) common to their molecules. The results of the agar gel-double-diffusion test also supported this conclusion.

A simplified radioimmunoassay technique for hydatid disease and human trichinosis

1981 ◽  
Vol 55 (1) ◽  
pp. 49-58 ◽  
Author(s):  
R. M. Matossian
Keyword(s):  
Comparative Study ◽  
Sensitivity And Specificity ◽  
Hydatid Disease ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Healthy Controls ◽  
Indirect Haemagglutination

AbstractA. A comparative study of a simplified radioimmunoassay (RIA) technique with the indirect haemagglutination (IHA) test in the diagnosis of 72 patients with hydatid disease indicated the greater sensitivity of the former. Both methods had an equal specificity in 60 control patients. RIA will serve as a useful addition to tests already available in hydatid serology.B. A comparison of the (RIA) technique with the fluorescent antibody test (FAT) in the diagnosis of 33 patients with early, active trichinosis and 30 healthy controls indicated an equal sensitivity and specificity of both methods. The adoption of RIA as an easier method is recommended.This study of a simplified radioimmunoassay technique for the diagnosis of hydatid disease and trichinosis in man is presented in two sections.

scholarly journals Efficacy of fluorescent antibody methods for detection of Pasteurella pestis in carcasses of albino laboratory mice stored for various periods

1962 ◽  
Vol 60 (4) ◽  
pp. 443-450 ◽  
Author(s):  
Bruce W. Hudson ◽  
Stuart F. Quan ◽  
Leo Kartman
Keyword(s):  
Infected Mouse ◽  
Guinea Pigs ◽  
Room Temperature ◽  
Fluorescent Antibody ◽  
Agar Plate ◽  
Antibody Test ◽  
Blood Agar Plate ◽  
Laboratory Mice ◽  
Cultural Methods ◽  
Antibody Tests

Fluorescent antibody techniques were used for detection of plague antigens in infected mouse carcasses stored at 4°–5° C., 20°–25° C. (room temperature) and 370° C. for periods as long as 340 days in certain cases. Tissues of two guinea-pigs infected with Pasteurella pestis were examined after 7 years storage at room temperature. The efficacy of the fluorescent antibody test was compared with blood agar plate culture and animal inoculation during periods in which viable plague bacilli were presumed to be present, and compared with Larson's modified Ascoli test for the presence of soluble plague antigens during periods in which plague bacteria were presumed to be non-viable.Fluorescent antibody tests were superior to animal inoculation or cultural methods in detection of P. pestis antigens in mouse carcasses stored for 4 days or longer at 37° C., for 8 days or longer at 20°–25° C. (room temperature), and for 34 days or longer at 4°–5° C. Upon examination of specimens presumed to lack viable plague organisms the fluorescent antibody test and precipitin tests were approximately equal in efficiency for detection of plague antigens in carcasses stored for 16–82 days at 37° C. and for 16–82 days at 20°–25° C. Ten of 21 mice stored for longer periods (140 and 340 days) at 4°–5° C. were negative by precipitin tests, whereas 19 of the same 21 rodents were positive by fluorescent antibody tests. In addition, after 7 years storage at room temperature tissues of two plague-infected guinea-pigs were negative by Larson's modified Ascoli test but positive by the fluorescent antibody test.

Chlamydia and Suspected Sexual Abuse

PEDIATRICS ◽  
1988 ◽  
Vol 81 (4) ◽  
pp. 601-602
Author(s):  
LAWRENCE S. NEINSTEIN ◽  
C. DANIEL FUSTER
Keyword(s):  
Sexual Abuse ◽  
Child Abuse ◽  
Young Child ◽  
Sensitivity And Specificity ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Prevalence Rates ◽  
Perinatal Infection ◽  
Direct Fluorescent Antibody ◽  
Low Prevalence

In Reply.— We thank Dr Hammerschlag for her thoughtful comments. We wholeheartedly agree with her about the use of cultures in the setting of suspected sexual abuse. As other studies have indicated, the sensitivity and specificity of the direct fluorescent antibody test are too low in a population with low prevalence rates such as a population with suspected child abuse. We also agree with Dr Hammerschlag about the possibility of perinatal infection in a young child.

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