Preliminary experiments on the in vitro cultivation of an amphistome Orthocoelium scoliocoelium (Trematoda: Digenea)

1982 ◽  
Vol 56 (3) ◽  
pp. 169-176 ◽  
Author(s):  
A. N. Sharma ◽  
P. N. Sharma
Keyword(s):  
Amino Acids ◽  
Egg Production ◽  
Rumen Fluid ◽  
Inorganic Salts ◽  
In Vitro Cultivation ◽  
Short Term ◽  
Physiological Alterations

ABSTRACTA medium containing inorganic salts, vitamins and amino-acids is described for the in vitro cultivation of the amphistome, Orthocoelium scoliocoelium, from the rumen of buffalo. Based on the properties of rumen fluid, this medium promoted and sustained normal egg production, at 37°C temperature and pH 7·4 to 7·8 for 15 days. In contrast to other media used, no patho-physiological alterations except abnormal cytoplasmic lipid, were detected. Since the parasites produced normal eggs, it is concluded that this medium is suitable for short-term cultivation of the parasites.

scholarly journals Oviposition by Schistosoma mansoni during in vitro cultivation

1996 ◽  
Vol 38 (6) ◽  
pp. 423-426 ◽  
Author(s):  
Leo Roberto Barth ◽  
Ana Paula Morais Fernandes ◽  
Vanderlei Rodrigues
Keyword(s):  
Schistosoma Mansoni ◽  
Egg Production ◽  
Culture Medium ◽  
Parasite Development ◽  
In Vitro Cultivation ◽  
Progressive Reduction ◽  
Maximum Period ◽  
Medium Time ◽  
Kinetics Of

Observation of Schistosoma mansoni oviposition during in vitro culture of adult worms for a maximum period of 10 days showed three well distinct phases in the kinetics of oviposition: an initial phase with low egg production, a period of maximum oviposition and finally a progressive reduction in the number of eggs during the late phases of culture. The kinetics of oviposition and the number of eggs laid by the parasites are influenced by the number of worm pairs per amount of RPMI 1640 medium, time of parasite development in the vertebrate host and type of serum utilized in the culture medium.

scholarly journals A Short Term in Vitro Cultivation of Babesia rodhaini and Babesia microti.

1995 ◽  
Vol 57 (5) ◽  
pp. 955-957 ◽  
Author(s):  
Sojin SHIKANO ◽  
Kenichi NAKADA ◽  
Rie HASHIGUCHI ◽  
Terumasa SHIMADA ◽  
Kenichiro ONO
Keyword(s):  
Babesia Microti ◽  
In Vitro Cultivation ◽  
Short Term

The usefulness of short-term in vitro cultivation for the detection and molecular study of Blastocystis hominis in stool specimens

2004 ◽  
Vol 93 (6) ◽  
Author(s):  
Sumeth Termmathurapoj ◽  
Saovanee Leelayoova ◽  
Pote Aimpun ◽  
Umaporn Thathaisong ◽  
Thirayost Nimmanon ◽  
...  
Keyword(s):  
Molecular Study ◽  
In Vitro Cultivation ◽  
Short Term ◽  
Blastocystis Hominis ◽  
Stool Specimens

scholarly journals Optimization of Conditions for In Vitro Culture of the Microphallid DigeneanGynaecotyla adunca

2014 ◽  
Vol 2014 ◽  
pp. 1-5 ◽  
Author(s):  
Jenna West ◽  
Alexandra Mitchell ◽  
Oscar J. Pung
Keyword(s):  
Egg Production ◽  
Horse Serum ◽  
Calf Serum ◽  
Culture Conditions ◽  
In Vitro Cultivation ◽  
Ilyanassa Obsoleta ◽  
Uca Pugnax ◽  
Newborn Calf Serum ◽  
Chicken Serum

In vitro cultivation of digeneans would aid the development of effective treatments and studies of the biology of the parasites. The goal of this study was to optimize culture conditions for the trematode,Gynaecotyla adunca. Metacercariae of the parasite from fiddler crabs,Uca pugnax, excysted in trypsin, were incubated overnight to permit fertilization, and were cultured in different conditions to find those that resulted in maximum worm longevity and egg production. When cultured in media lacking serum, worms lived longer in Hanks balanced salt solution and Dulbecco’s Modified Eagle medium/F-12 (DME/F-12) than in RPMI-1640 but produced the most eggs in DME/F-12. Worm longevity and egg production increased when worms were grown in DME/F-12 supplemented with 20% chicken, horse, or newborn calf serum but the greatest number of eggs was deposited in cultures containing horse or chicken serum. Horse serum was chosen over chicken serum due to the formation of a precipitate in chicken serum. The optimal concentration of horse serum with respect to egg production ranged from 5 to 20%. Infectivity of eggs deposited by worms in culture was tested by feeding eggs to mud snails,Ilyanassa obsoleta. None of these snails producedG. aduncacercariae.

scholarly journals Bis(2-ethylhexyl) phtalate affects spermatozoa motility during short-term in vitro cultivation

2015 ◽  
Vol 04 (Special issue 2) ◽  
pp. 73-75 ◽  
Author(s):  
Jana Lukáčová ◽  
Tomáš Jambor ◽  
Zuzana Kňažická ◽  
Eva Tvrdá ◽  
Norbert Lukáč
Keyword(s):  
In Vitro Cultivation ◽  
Short Term ◽  
Spermatozoa Motility

Preliminary Experiments with a Defined Culture Medium for Larval Fasciola hepatica

1973 ◽  
Vol 47 (2) ◽  
pp. 181-189 ◽  
Author(s):  
R. S. V. Pullin
Keyword(s):  
Amino Acids ◽  
Culture Medium ◽  
Fasciola Hepatica ◽  
Dry Weight ◽  
Defined Medium ◽  
Inorganic Salts ◽  
Final Maturation ◽  
Defined Culture ◽  
Stock Solutions

A defined medium is described as a basis for in vitro culture work with larval Fasciola hepatica. This medium, termed BCM, can be quickly made up by using a system of stock solutions. BCM contains inorganic salts, glucose, amino acids, vitamins and antibiotics, but no lipid or proteins. Rediae can be dissected from infected snails for culture, but many appear to be contaminated with bacteria. Large rediae cannot survive in BCM but free immature cercariae can complete their final maturation in vitro. This final maturation, from the 30th to the 35th day after miracidial penetration of donor snails, includes tail growth and appearance of body pigmentation. Cercariae matured in vitro encyst successfully when transferred from BCM to water. Small rediae survive in BCM for 5 days, but show no growth or development measured as dry weight and total nitrogen.

scholarly journals Idendification of sulphur-rich proteins which resist rumen degradation and are hydrolysed rapidly by intestinal proteases

1994 ◽  
Vol 72 (6) ◽  
pp. 855-863 ◽  
Author(s):  
Kerrie R. Hancock ◽  
Paul M. Ealing ◽  
Derek W.R. White
Keyword(s):  
Amino Acids ◽  
Rumen Fluid ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Essential Amino Acids ◽  
Rumen Degradation ◽  
Foreign Proteins ◽  
Sheep Rumen ◽  
Pasture Plants

Several proteins with high proportions of S-containing essential amino acids were incubated in sheep rumen fluid in vitro and their rate of digestion was examined by sodium dodecyl sulphate-polyacrylamide-gel electrophoresis. The S-rich proteins rice prolamin (10 kDa), maize zein (10 kDa) and the 3·2 kDa pumpkin (Cucurbita maxima L.) trypsin inhibitor-1 (CMTI-1) were highly resistant to rumen fluid degradation, relative to control proteins of known degradation rate (casein, bovine serum albumin (BSA) and pea (Pisum sativum) albumin-1 (PA1)). Comparison of PA1 and a recombinant N-terminal epitope-tagged PA1 indicated that addition of the epitope caused a slight increase in resistance to rumen degradation. The proteins were also incubated with a mixture of trypsin (EC 3·4·21·4) and chymotrypsin (EC 3·4·21.1). PA1, BSA and casein were hydrolysed less rapidly than rice prolamin, maize zein and CMTI-1. Digestion by these intestinal proteases appeared to be complete. Thus, the prolamin, zein and CMTI-1 proteins are suitable candidates for expression as foreign proteins in pasture plants to increase throughput and uptake of essential amino acids in sheep.

A method for the short-term in vitro cultivation of the chick embryo on liquid media

In Vitro ◽  
1973 ◽  
Vol 8 (5) ◽  
pp. 383-385 ◽  
Author(s):  
Robert C. Fitzsimmons ◽  
Donna E. Mason
Keyword(s):  
Chick Embryo ◽  
In Vitro Cultivation ◽  
Liquid Media ◽  
Short Term

PROTEIN BIOSYNTHESIS IN VITRO BY THE PARS INTERMEDIA OF THE MOUSE PITUITARY GLAND

1977 ◽  
Vol 72 (2) ◽  
pp. 173-179
Author(s):  
H. ISHERWOOD ◽  
V. F. THORNTON
Keyword(s):  
Amino Acids ◽  
Molecular Weight ◽  
Protein Biosynthesis ◽  
Pars Intermedia ◽  
Secretory Product ◽  
Posterior Pituitary ◽  
Short Term ◽  
Pituitary Glands ◽  
Mouse Pituitary

SUMMARY During short-term incubations of isolated posterior pituitary glands of the mouse, isotopically labelled amino acids were incorporated into protein by the cells of the pars intermedia. Using labelled leucine, 5–10% of incorporated label was found in a protein (P1), with a molecular weight of about 75000. Protein P1 could be isolated from both fresh and incubated tissue, and was a normal and indeed major, secretory product of the pars intermedia, constituting more than 50% of the protein present.

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