A method for the short-term in vitro cultivation of the chick embryo on liquid media

In Vitro ◽  
1973 ◽  
Vol 8 (5) ◽  
pp. 383-385 ◽  
Author(s):  
Robert C. Fitzsimmons ◽  
Donna E. Mason
Keyword(s):  
Chick Embryo ◽  
In Vitro Cultivation ◽  
Liquid Media ◽  
Short Term

Special Problems of Experimenting in ovo on the Early Chick Embryo, and a Solution

Development ◽  
1960 ◽  
Vol 8 (4) ◽  
pp. 369-375
Author(s):  
P. H. S. Silver
Keyword(s):  
Chick Embryo ◽  
Short Term ◽  
Stages Of Development ◽  
In Ovo ◽  
Technical Problems ◽  
Early Chick Embryo ◽  
In Vitro Methods ◽  
Early Stages

It seems to be generally accepted that experimenting in ovo on the chick during the early stages of development (up to about 48 hours) is fraught with the greatest difficulty. After about this time no serious technical problems arise and a high proportion of successful results can be expected. It is natural to ask why there should be this change-over from extreme difficulty to reasonable simplicity. New (1955) attributed to this ‘inaccessibility of the chick embryo in the egg’ the invention of his own and many other in vitro methods during the last 30 years. There is no doubt that, when short-term experiments only are required, in vitro methods will probably always be preferred. But all in vitro methods suffer from the disadvantage that the embryo cannot be expected to survive for more than 48 hours or so after explantation.

scholarly journals A Short Term in Vitro Cultivation of Babesia rodhaini and Babesia microti.

1995 ◽  
Vol 57 (5) ◽  
pp. 955-957 ◽  
Author(s):  
Sojin SHIKANO ◽  
Kenichi NAKADA ◽  
Rie HASHIGUCHI ◽  
Terumasa SHIMADA ◽  
Kenichiro ONO
Keyword(s):  
Babesia Microti ◽  
In Vitro Cultivation ◽  
Short Term

The usefulness of short-term in vitro cultivation for the detection and molecular study of Blastocystis hominis in stool specimens

2004 ◽  
Vol 93 (6) ◽  
Author(s):  
Sumeth Termmathurapoj ◽  
Saovanee Leelayoova ◽  
Pote Aimpun ◽  
Umaporn Thathaisong ◽  
Thirayost Nimmanon ◽  
...  
Keyword(s):  
Molecular Study ◽  
In Vitro Cultivation ◽  
Short Term ◽  
Blastocystis Hominis ◽  
Stool Specimens

Preliminary experiments on the in vitro cultivation of an amphistome Orthocoelium scoliocoelium (Trematoda: Digenea)

1982 ◽  
Vol 56 (3) ◽  
pp. 169-176 ◽  
Author(s):  
A. N. Sharma ◽  
P. N. Sharma
Keyword(s):  
Amino Acids ◽  
Egg Production ◽  
Rumen Fluid ◽  
Inorganic Salts ◽  
In Vitro Cultivation ◽  
Short Term ◽  
Physiological Alterations

ABSTRACTA medium containing inorganic salts, vitamins and amino-acids is described for the in vitro cultivation of the amphistome, Orthocoelium scoliocoelium, from the rumen of buffalo. Based on the properties of rumen fluid, this medium promoted and sustained normal egg production, at 37°C temperature and pH 7·4 to 7·8 for 15 days. In contrast to other media used, no patho-physiological alterations except abnormal cytoplasmic lipid, were detected. Since the parasites produced normal eggs, it is concluded that this medium is suitable for short-term cultivation of the parasites.

scholarly journals Bis(2-ethylhexyl) phtalate affects spermatozoa motility during short-term in vitro cultivation

2015 ◽  
Vol 04 (Special issue 2) ◽  
pp. 73-75 ◽  
Author(s):  
Jana Lukáčová ◽  
Tomáš Jambor ◽  
Zuzana Kňažická ◽  
Eva Tvrdá ◽  
Norbert Lukáč
Keyword(s):  
In Vitro Cultivation ◽  
Short Term ◽  
Spermatozoa Motility

Cells on Porous Substrates In Vitro and In Vivo

1974 ◽  
Vol 96 (2) ◽  
pp. 131-137
Author(s):  
K. D. Ansevin ◽  
H. G. Welgus ◽  
C. A. Homsy ◽  
B. V. Lipps
Keyword(s):  
Connective Tissue ◽  
Porous Matrix ◽  
Polymer Materials ◽  
Organic Polymer ◽  
Gelatin Sponge ◽  
In Vitro Cultivation ◽  
Short Term ◽  
Tissue Cells

Disaggregated cells of kidney, lung and liver of 14-day-old chick embryos were cultivated in vitro on several open-pore, organic-polymer sponges and on one gelatin sponge; in some cases this was followed by short-term, subcutaneous implantation into rats. In order to study invasion of connective tissue cells into the porous matrix, the same organic-polymer materials were implanted into rats, and in one experimental series this was followed by in vitro cultivation after they had been retrieved from the animals. The results showed that: 1) epithelial cells have different requirements for invasion and histogenesis from those of connective tissue cells; 2) the type of porous substrate influences morphology of connective tissue that forms within it.

Ultrastructural investigations of MDL 19,660-induced effects on the canine platelet and megakaryocyte

1990 ◽  
Vol 48 (3) ◽  
pp. 374-375
Author(s):  
D.E. Loudy ◽  
J. Sprinkle-Cavallo ◽  
J.T. Yarrington ◽  
F.Y. Thompson ◽  
J.P. Gibson
Keyword(s):  
Antidepressant Drug ◽  
Beagle Dogs ◽  
Long Term Effects ◽  
Short Term ◽  
Platelet Counts ◽  
Toxicological Studies ◽  
Induced Aggregation ◽  
Internal Architecture

Previous short term toxicological studies of one to two weeks duration have demonstrated that MDL 19,660 (5-(4-chlorophenyl)-2,4-dihydro-2,4-dimethyl-3Hl, 2,4-triazole-3-thione), an antidepressant drug, causes a dose-related thrombocytopenia in dogs. Platelet counts started to decline after two days of dosing with 30 mg/kg/day and continued to decrease to their lowest levels by 5-7 days. The loss in platelets was primarily of the small discoid subpopulation. In vitro studies have also indicated that MDL 19,660: does not spontaneously aggregate canine platelets and has moderate antiaggregating properties by inhibiting ADP-induced aggregation. The objectives of the present investigation of MDL 19,660 were to evaluate ultrastructurally long term effects on platelet internal architecture and changes in subpopulations of platelets and megakaryocytes.Nine male and nine female beagle dogs were divided equally into three groups and were administered orally 0, 15, or 30 mg/kg/day of MDL 19,660 for three months. Compared to a control platelet range of 353,000- 452,000/μl, a doserelated thrombocytopenia reached a maximum severity of an average of 135,000/μl for the 15 mg/kg/day dogs after two weeks and 81,000/μl for the 30 mg/kg/day dogs after one week.

The Effect of Dimethyl Sulfoxide on In Vitro Platelet Function

1976 ◽  
Vol 36 (01) ◽  
pp. 221-229 ◽  
Author(s):  
Charles A. Schiffer ◽  
Caroline L. Whitaker ◽  
Morton Schmukler ◽  
Joseph Aisner ◽  
Steven L. Hilbert
Keyword(s):  
Platelet Count ◽  
Platelet Aggregation ◽  
Dimethyl Sulfoxide ◽  
Platelet Function ◽  
Platelet Volume ◽  
Short Term ◽  
Platelet Factor ◽  
Short Term Exposure ◽  
Structural Abnormalities

SummaryAlthough dimethyl sulfoxide (DMSO) has been used extensively as a cryopreservative for platelets there are few studies dealing with the effect of DMSO on platelet function. Using techniques similar to those employed in platelet cryopreservation platelets were incubated with final concentrations of 2-10% DMSO at 25° C. After exposure to 5 and 10% DMSO platelets remained discoid and electron micrographs revealed no structural abnormalities. There was no significant change in platelet count. In terms of injury to platelet membranes, there was no increased availability of platelet factor-3 or leakage of nucleotides, 5 hydroxytryptamine (5HT) or glycosidases with final DMSO concentrations of 2.5, 5 and 10% DMSO. Thrombin stimulated nucleotide and 5HT release was reduced by 10% DMSO. Impairment of thrombin induced glycosidase release was noted at lower DMSO concentrations and was dose related. Similarly, aggregation to ADP was progressively impaired at DMSO concentrations from 1-5% and was dose related. After the platelets exposed to DMSO were washed, however, aggregation and release returned to control values. Platelet aggregation by epinephrine was also inhibited by DMSO and this could not be corrected by washing the platelets. DMSO-plasma solutions are hypertonic but only minimal increases in platelet volume (at 10% DMSO) could be detected. Shrinkage of platelets was seen with hypertonic solutions of sodium chloride or sucrose suggesting that the rapid transmembrane passage of DMSO prevented significant shifts of water. These studies demonstrate that there are minimal irreversible alterations in in vitro platelet function after short-term exposure to DMSO.

EFFECT OF ACTINOMYCIN D ON TSH-INDUCED STIMULATION OF THYROIDAL PROTEIN SYNTHESIS IN THE RAT

1974 ◽  
Vol 77 (1) ◽  
pp. 64-70 ◽  
Author(s):  
Gustav Wägar
Keyword(s):  
Protein Synthesis ◽  
Actinomycin D ◽  
The Other ◽  
Leucine Incorporation ◽  
Short Term ◽  
Other Hand ◽  
Thyroid Glands ◽  
Translational Level ◽  
Stimulation Of

ABSTRACT Whether the short-term regulation of thyroidal protein synthesis by TSH occurs at the transcriptional or the translational level was tested by measuring the effect of actinomycin D (act D) on the TSH-induced stimulation of L-14C-leucine incorporation into the thyroidal proteins of rats. TSH was injected 6 h before the rats were killed. The thyroid glands were then removed and incubated in vitro in the presence of L-14C-leucine for 2 h. The pronounced stimulation of leucine incorporation in the TSH-treated animals was depressed as compared with controls but still significant even when the animals had been pre-treated with 100 μg act D 24 and 7 h before sacrifice. On the other hand, act D strongly decreased incorporation of 3H-uridine into RNA. Short-term regulation of thyroidal protein synthesis by TSH appears to be partly but not wholly dependent on neosynthesis of RNA. Hence regulation may partly occur at the translation level of protein synthesis.

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