scholarly journals A serological relationship between mumps and sendai viruses

1960 ◽  
Vol 58 (3) ◽  
pp. 283-289 ◽  
Author(s):  
P. S. Gardner
Keyword(s):  
Blood Transfusion ◽  
General Hospital ◽  
Virus Particle ◽  
Blood Donor ◽  
Sendai Virus ◽  
Mumps Virus ◽  
Serological Relationship ◽  
Essential Components ◽  
Antigen Complex

Sixty-six paired sera from patients suffering from mumps were examined with Sendai and mumps virus particle antigen: 50% showed a rise of complementfixing antibodies to mumps virus particle antigen only and the rest to both antigens.This relationship was further substantiated by the occurrence of both antibodies in a proportion of the sera of 362 blood transfusion donors and 978 sera from antenatal patients.The occurrence of both antibodies in patients' sera is discussed and the conclusion is reached that one of the components of the mumps antigen complex is indistinguishable from the essential components of the Sendai virus.I am indebted to the Maternity Department of the Edgware General Hospital for supplying the antenatal sera, and to Dr Preston of the Regional Transfusion Centre, Oxford, for the blood donor sera.

scholarly journals Structure and assembly of double-headed Sendai virus nucleocapsids

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Na Zhang ◽  
Hong Shan ◽  
Mingdong Liu ◽  
Tianhao Li ◽  
Rui Luo ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Measles Virus ◽  
Sendai Virus ◽  
Nipah Virus ◽  
Mumps Virus ◽  
Genome Replication ◽  
Direct Visualization ◽  
Closed State ◽  
Cryo Electron Microscopy ◽  
Negative Sense

AbstractParamyxoviruses, including the mumps virus, measles virus, Nipah virus and Sendai virus (SeV), have non-segmented single-stranded negative-sense RNA genomes which are encapsidated by nucleoproteins into helical nucleocapsids. Here, we reported a double-headed SeV nucleocapsid assembled in a tail-to-tail manner, and resolved its helical stems and clam-shaped joint at the respective resolutions of 2.9 and 3.9 Å, via cryo-electron microscopy. Our structures offer important insights into the mechanism of the helical polymerization, in particular via an unnoticed exchange of a N-terminal hole formed by three loops of nucleoproteins, and unveil the clam-shaped joint in a hyper-closed state for nucleocapsid dimerization. Direct visualization of the loop from the disordered C-terminal tail provides structural evidence that C-terminal tail is correlated to the curvature of nucleocapsid and links nucleocapsid condensation and genome replication and transcription with different assembly forms.

Renal transplantation: HLA-linked, non-cytotoxic antibodies develop after blood transfusion

1988 ◽  
Vol 74 (4) ◽  
pp. 385-388 ◽  
Author(s):  
Alison M. MacLeod ◽  
Keith N. Stewart ◽  
Stanislaw Urbaniak ◽  
Graeme R. D. Catto
Keyword(s):  
Blood Transfusion ◽  
B Lymphocytes ◽  
Blood Donor ◽  
Hla Antigens ◽  
Fc Receptor ◽  
Antibody Activity ◽  
Receptor Blocking ◽  
Single Donor ◽  
Before And After ◽  
Blocking Antibodies

1. A previously untransfused dialysis patient was given blood from a single donor on three occasions and sera were obtained from the recipient before and after transfusion. 2. The sera were tested against B-lymphocytes from the blood donor and his family in the erythrocyte–antibody rosette inhibition assay to determine whether any Fc-receptor-blocking activity which developed was HLA linked. 3. Antibody activity was noted after the second transfusion and was directed toward B-lymphocytes from the blood donor and his family members sharing the haplotype HLA A11, B12 with him. 4. These antibodies were removed by the absorption of active sera with lymphocytes from the blood donor but not from (a) his sibling who shared no HLA antigens with him or (b) the transfusion recipient. Absorption of the active sera with platelets from the blood donor did not remove activity. 5. These results indicate that Fc-receptor-blocking antibodies developing after blood transfusion are directed to HLA-linked antigens on donor lymphocytes.

scholarly journals COLICINE K

1958 ◽  
Vol 108 (5) ◽  
pp. 731-752 ◽  
Author(s):  
Tsunehisa Amano ◽  
Walther F. Goebel ◽  
Elizabeth Miller Smidth
Keyword(s):  
Neutralizing Antibody ◽  
Protein Component ◽  
The Other ◽  
Serological Relationship ◽  
E Coli ◽  
O Antigen ◽  
Two Agents ◽  
Antigen Complex

By immunological means it has been shown that colicine K is associated with the O antigen of the colicinogenic bacillus E. coli K235 L+OC+. The colicine K-O antigen complex elicits the formation of at least two types of antibodies, one a precipitin, the other a colicine-neutralizing antibody. The first precipitates colicine K without neutralizing it, the second neutralizes the colicine without precipitating it. Unlike the purified colicine K complex, the colicine protein component of the O antigen is precipitable by the neutralizing antibody. There is no demonstrable serological relationship between colicine K and phage T6. These two agents must be considered to be separate and distinct entities.

Satisfaction survey in general hospital personnel involved in blood transfusion: implementation of the ISO 9001: 2000 standard

2004 ◽  
Vol 11 (4) ◽  
pp. 177-182 ◽  
Author(s):  
S. Chord-Auger ◽  
E. Tron de Bouchony ◽  
M.C. Moll ◽  
D. Boudart ◽  
G. Folléa
Keyword(s):  
Blood Transfusion ◽  
General Hospital ◽  
Iso 9001 ◽  
Satisfaction Survey ◽  
Hospital Personnel

scholarly journals Characterization of Sendai virus M protein mutants that can partially interfere with virus particle production

1999 ◽  
Vol 80 (11) ◽  
pp. 2977-2986 ◽  
Author(s):  
Geneviève Mottet ◽  
Virginie Müller ◽  
Laurent Roux
Keyword(s):  
Virus Particle ◽  
Particle Production ◽  
Sendai Virus ◽  
Single Band ◽  
M Protein ◽  
Phenotypic Traits ◽  
Cell Periphery ◽  
Page Migration ◽  
Protein Mutants ◽  
Virus Particle Production

Substitution of Val113 in Sendai virus (SeV) M protein generates non-functional polypeptides, characterized by their exclusion from virus particles and by their ability to interfere with virus particle production. These phenotypic traits correlate with a single-band PAGE migration profile, in contrast to wild-type M (Mwt ), which separates into two species, one of which is a phosphorylated form. The single-band migration is likely to result from a conformational change, as evidenced by the lack of maturation of a native epitope and by a particular tryptic digestion profile, and not from the phosphorylation of all M molecules, an assumption consistent with the PAGE migration feature. One of the M mutants (HA–M30 , an M protein carrying Thr112Met and Val113 Glu substitutions tagged with an influenza virus haemagglutinin epitope) was characterized further in the context of SeV infection, i.e. under conditions of co-expression with Mwt. HA–M 30 is shown (i) to bind mainly to membrane fractions, (ii) not to co-precipitate Mwt, as HA–Mwt does, (iii) to interfere with the binding of nucleocapsids to membranes and (iv) to accumulate in perinuclear regions, in contrast to HA-Mwt , which is also found at the cell periphery. Such mutants constitute potential tools for the identification of critical steps in paramyxovirus assembly and budding.

scholarly journals Blood transfusion transmitted infectious diseases (HIV, HBV, HCV, Syphilis and Malaria) among the voluntary blood donors in Dhaka, Bangladesh

2015 ◽  
Vol 42 (1) ◽  
pp. 77-84
Author(s):  
Hamida Khanum ◽  
Mafruha Khanom ◽  
Hashina Banu ◽  
Farhana Muznebin ◽  
Shahela Alam ◽  
...  
Keyword(s):  
Blood Transfusion ◽  
Infectious Diseases ◽  
Blood Donor ◽  
Malaria Infection ◽  
Blood Donors ◽  
Hbv Infection ◽  
Hiv Positive ◽  
Age Group ◽  
Voluntary Blood Donor ◽  
The Difference

Prevalence of five important blood transfusion-transmitted infectious diseases (HIV, HBV, HCV, Syphilis, and Malaria) were studied among the voluntary blood donors in Dhaka. Out of 1500 donors, positive infections was identified in 99 of cases and rest of the donors were screened negative. Male voluntary blood donor were 82% and only 18% were female. About 53.53% male infectious blood donor had HBV infection whereas, 26.26% female had HBV. The difference was significant (P < 0.05). In age group of 18-24 years, 36% of the donor had HBV infection and HCV was found positive in 04.04% of student. Among the students, HBV was positive in 44%. Only 01.01% of teacher was HIV positive. Malaria infection was positive only among the business man (01.01%). The positive cases (01.01%) with TPHA found among the students, teacher and worker and the difference was significant (P<0.05). 40.40% of the volunteer blood donors were illiterate and 24.24% donor had Master degree and 8% HSC passed.Bangladesh J. Zool. 42(1): 77-84, 2014

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