Reliability of conventional ear swabs in tubotympanic CSOM

AbstractConventional external ear swab cultures were compared with cultures obtained from the middle ear, in 25 cases of tubotympanic type (‘safe’), chronic suppurative otitis media. These cultures were identical in 22 of the 25 cases.Anaerobic organisms were isolated in only one case. Mixed infections of both gram-positive and gramnegative organisms were seen in nine cases (36 per cent).Staphylococcus aureus was the commonest single agent isolated (16 cases). Multidrug resistance was seen in 12 of these isolates.

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Correlation between histomorphometric changes and the type of aerobic bacteria isolated in chronic suppurative otitis media

Vojnosanitetski pregled ◽

10.2298/vsp1101046z ◽

2011 ◽

Vol 68 (1) ◽

pp. 46-50

Author(s):

Emilija Zivkovic-Marinkov ◽

Milan Stankovic ◽

Dragan Mihailovic ◽

Mila Bojanovic

Keyword(s):

Staphylococcus Aureus ◽

Otitis Media ◽

Middle Ear ◽

Granulation Tissue ◽

Bacterial Flora ◽

Inflammatory Cells ◽

Chronic Suppurative Otitis Media ◽

Aerobic Bacteria ◽

Middle Ear Mucosa ◽

Suppurative Otitis Media

Background/Aim. Bacterial flora is a very important factor in pathogenesis of chronic suppurative otitis media (CSOM) and significantly influences the type and intensity of osteolytic process. There are few histomorphometric investigations of middle ear mucosa in chronic otitis. The aim of this study was to identify aerobic bacteria responsible for chronic suppurative otitis media as well as their association with histomorphometric changes of middle ear mucosa. Methods. A prospective study that comprised 153 patients treated in the Clinc for Ear, Thorat and Nose Diseases, Nis, was conducted. Bacteriologic analysis of diseased ear secretion was carried out in all patients. Intraoperatively removed granulation tissue was used for histomorphometry. The analysed parameters were: the number of inflammatory cells, as well as vascularization and vasodilatation. Results. The most frequently isolated aerobic bacteria from chronic suppurative otitis media were Staphylococcus aureus (29.02%), Pseudomonas aeruginosa (29.02%) and Proteus spp. (21.76%). There was no correlation between the type of pathologic process and the type of bacteria.The number of inflammatory cells in the granulation tissue in pure cultures of Staphylococcus aureus was 1,597.33 ? 549.45 and in Pseudomonas auriginosa cultures was 2,639 ? 648. Conclusion. This study showed that there is a statistically significant correlation between the number of inflammatory cells in the granulation tissue and the type of aerobic bacteria we isolated. The intensity of the infection in chronic suppurative otitis media depends on the type of the isolated bacteria, which emphasizes the importance of adequate preoperative antimicrobial therapy.

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Bacteriological Profile of Chronic Suppurative Otitis Media and Antibiotic Susceptibility in a Tertiary Care Hospital in Shanghai, China

Ear Nose & Throat Journal ◽

10.1177/0145561320923823 ◽

2020 ◽

pp. 014556132092382

Author(s):

Jianghong Xu ◽

Qiang Du ◽

Yilai Shu ◽

Jian Ji ◽

Chunfu Dai

Keyword(s):

Staphylococcus Aureus ◽

Otitis Media ◽

Middle Ear ◽

Antibiotic Susceptibility ◽

Tertiary Care Hospital ◽

Tertiary Care ◽

Chronic Suppurative Otitis Media ◽

Care Hospital ◽

Suppurative Otitis Media ◽

Bacteriological Profile

Objective: Chronic suppurative otitis media (CSOM) is mostly caused by bacterial infection of the middle ear and antibiotics are generally used empirically, which may lead to the emergence of resistant bacterial strains. The objective of the study is to assess the bacteriological profile of and evaluate the antibiotic susceptibility of strains isolated in a tertiary care hospital in Shanghai, China. Methods: This study included 289 individuals with clinical diagnosis of CSOM. Middle ear purulent discharge was obtained with sterile swabs and cultured for bacterial pathogens. The susceptibility of the isolated microorganisms to antibiotics was assessed by a microdilution method. Results: Bacterial pathogens were found in 223 (77.2%) of the 289 cases. A total of 236 strains were isolated. Staphylococcus aureus was the commonest bacteria (44.9%) followed by Pseudomonas aeruginosa (16.9%) and coagulase-negative Staphylococcus (8.5%). There were 18.9% methicillin-resistant S aureus (MRSA) among the obtained S aureus organisms. Multidrug-resistant P aeruginosa was found in 4 patients, making up 10% of all detected P aeruginosa. Staphylococcus aureus showed highest susceptibility to vancomycin (100%), then gentamicin (98.1%) and rifampicin (97.2%) and was most resistant to penicillin (61.3%) and erythromycin (50%). All isolated P aeruginosa showed susceptibility to piperacillin, piperacillin/tazobactam, and meropenem. High degree of resistance in P aeruginosa was observed toward levofloxacin (42.5%), ciprofloxacin (40%), and ceftriaxone (30%). Conclusion: The high prevalence of MRSA and fluoroquinolone-resistant P aeruginosa indicated cephalosporins and fluoroquinolone as primary empirical antibiotic drugs in CSOM to be cautiously used. In order to reduce the incidence of resistant strains and promote effective usage of antibiotics, all aural discharges should be cultured to determine antibacterial susceptibility patterns before treatment.

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Prevalence and antibiotic sensitivity of Staphylococcus aureus and Pseudomanas aeruginosa in middle ear fluids of chronic suppurative otitis media and chronic rhinosinusitis patients undergoing ear surgery

International Journal of Infectious Diseases ◽

10.1016/j.ijid.2016.02.264 ◽

2016 ◽

Vol 45 ◽

pp. 101-102 ◽

Cited By ~ 1

Author(s):

J. Madhavi ◽

M. Santoshi ◽

K.R. Meghanadh ◽

A. Jyothy

Keyword(s):

Staphylococcus Aureus ◽

Chronic Rhinosinusitis ◽

Otitis Media ◽

Middle Ear ◽

Antibiotic Sensitivity ◽

Chronic Suppurative Otitis Media ◽

Ear Surgery ◽

Suppurative Otitis Media

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Bacteriological study and antibiotic sensitivity profile in patients with ear discharge visiting ENT OPD at tertiary care center

International Journal of Otorhinolaryngology and Head and Neck Surgery ◽

10.18203/issn.2454-5929.ijohns20175632 ◽

2017 ◽

Vol 4 (1) ◽

pp. 237

Author(s):

Kailas R. Patel ◽

Alpesh D. Fefar ◽

Paresh J. Khavdu ◽

Sejal N. Mistry ◽

Manish R. Mehta

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Otitis Media ◽

Middle Ear ◽

Chronic Otitis Media ◽

Antibiotic Sensitivity ◽

Polymyxin B ◽

Chronic Suppurative Otitis Media ◽

Purulent Discharge ◽

Suppurative Otitis Media

Background: Otitis media (OM) is a big menace in countries like India. Chronic suppurative otitis media (CSOM) is chronic inflammation of middle ear, which affects the tympanic membrane, middle ear mucosa and other middle ear structures characterised by the presence of persistent perforation or persistent mucoid or muco-purulent discharge for at least 8 weeks. Variety of organisms founds in patients suffering from chronic otitis media like, Pseudomonas, Staphylococcus aureus, Proteus mirabilis, Klebsiella pneumonia and Escherichia coli found. The pattern of organisms varies in different geographical areas. The objectives of the study were to isolate bacteria from patients with ear discharge, to find out antibiotic sensitivity of isolated organismsMethods: This is an observational cross sectional study done in the OPD department of otorhinolaryngology, at P.D.U medical college and hospital, Rajkot, for a period of six months from January 2017 to June 2017. A total number 200 patients of both gender between 5 to 74 years who visited the OPD department of otorhinolaryngology with chronic ear discharge taken for study. Results: Out of 200 patients of 5-74 years with chronic suppurative otitis media were examined and out of this Hundred and twelve (54%) of the patients were males with mean age of 33.44. Pseudomonas aeruginosa was the commonest identified organisms the sensitivity pattern highly favoured Pipercillin, Amikacin and Polymyxin B, Cefoparazone. Conclusions: Chronic otitis media is more prevalent in males than females. Commonest organisms found were Pseudomonas aeruginosa followed by Staphylococcus aureus. Piperacillin, Amikacin, Gentamicin, Polymyxin B and Cefoperazone were found most effective agents against Pseudomonas aeruginosa.

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Prevalence and antibiotic susceptibility of Pseudomonas aeruginosa isolated from chronic suppurative otitis media

International Journal of Otorhinolaryngology and Head and Neck Surgery ◽

10.18203/issn.2454-5929.ijohns20164494 ◽

2016 ◽

Vol 3 (1) ◽

pp. 56

Author(s):

Kiran A. Deshmukh ◽

Deepak Manthale

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Otitis Media ◽

Middle Ear ◽

Antibiotic Susceptibility ◽

Tertiary Care ◽

Chronic Suppurative Otitis Media ◽

Care Hospital ◽

Suppurative Otitis Media ◽

Production Characteristic

Background: Chronic suppurative otitis media (CSOM) is the most common middle ear infection characterized by recurrent ear discharges or otorrhoea through a tympanic perforation from the middle ear. The organisms isolated from CSOM include both aerobic and anaerobic bacteria. Among the bacteria, Pseudomonas aeruginosa (P. aeruginosa) has been particularly blamed for deep seated and progressive destruction of middle ear and mastoid structures through its toxins and enzymes. The present study was done to determine the prevalence and antibiotic susceptibility of P. aeruginosa isolated from CSOM.Methods: This was a retrospective study conducted in a tertiary care hospital in South India in March 2016. Data was obtained from the microbiology records from January 2014 to January 2016. Patients who had active ear discharge for at least three months were included in the study. P. aeruginosa was identified by colony morphology, pigment production, characteristic musty or earthy odor. The identification was confirmed by motility and standard biochemical tests. Results: A total of 293 cases of CSOM were included in the study. Microbial growth was seen in 248 (84.6%) cases and remaining 45 cases did not show any growth. Among 248 bacterial growth, 87 (35%) were positive for P. aeruginosa. Most common organism was P. aeruginosa followed by Staphylococcus aureus. Highest resistance was seen with Gentamicin and Ofloxacin and least resistance with Imipenem. The increased resistance to Cephalosporin group is warning sign, as these are potent anti-Psuedomals.Conclusions: P. aeruginosa and Staphylococcus aureus were found to be the common cause of CSOM in the present study. P. aeruginosa was resistant to commonly used antimicrobials. There was no resistance seen with Imipenem and only two strains were resistant to Piperacillin+tazobactum. Regular evaluation of the microbiological pattern and antibiogram of isolates will help in decreasing the complications of CSOM.

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Bacteriological profile of chronic suppurative otitis media in a tertiary care hospital

International Journal of Otorhinolaryngology and Head and Neck Surgery ◽

10.18203/issn.2454-5929.ijohns20173031 ◽

2017 ◽

Vol 3 (3) ◽

pp. 601 ◽

Cited By ~ 5

Author(s):

Sowmya Tumkur Rangaiah ◽

Ravi Dudda ◽

M. Hanumanth Prasad ◽

Nagavara Kalegowda Balaji ◽

Sumangala B. ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Otitis Media ◽

Middle Ear ◽

Antibiotic Susceptibility ◽

Chronic Suppurative Otitis Media ◽

Care Hospital ◽

Suppurative Otitis Media ◽

Sensitivity Pattern ◽

Culture Sensitivity

Background: Chronic suppurative otitis media is a disease affecting the middle ear and mastoid cavity, leading to considerable morbidity by causing hearing loss; and even life threatening complications in squamosal type of CSOM, if left untreated over long periods of time. Hence it is imperative to treat the persistent ear discharge in CSOM according to the antibiotic susceptibility of the organisms isolated. Methods: This is a prospective cross sectional study done in 135 patients of chronic suppurative otitis media presenting with active ear discharge. Sterile swabs were used to collect pus from discharging ear and sent for culture sensitivity. Results: Staphylococcus and Pseudomonas aeruginosa were the most common organisms isolated in this study. Pseudomonas aeruginosa was most sensitive to Piperacillin-Tazobactam, followed by Meropenem and Ciprofloxacin. Staphylococcus aureus was most sensitive to Linezolid followed by Cefoxitin and Erythromycin. Conclusions: Treating active discharge of CSOM according to pus culture sensitivity pattern reduces the bacterial load in the middle ear and makes the ear more favourable for graft uptake. Keywords: Chronic suppurative otitis media, Aerobic bacterial culture, Antibiotic susceptibility, Pseudomonas aeruginosa, Staphylococcus aureus

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Comparison of Arbekacin and Vancomycin in Treatment of Chronic Suppurative Otitis Media by Methicillin Resistant Staphylococcus aureus

Journal of Korean Medical Science ◽

10.3346/jkms.2015.30.6.688 ◽

2015 ◽

Vol 30 (6) ◽

pp. 688 ◽

Cited By ~ 6

Author(s):

Ji-Hee Hwang ◽

Ju-Hyung Lee ◽

Jeong-Hwan Hwang ◽

Kyung Min Chung ◽

Eun-Jung Lee ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Otitis Media ◽

Methicillin Resistant Staphylococcus Aureus ◽

Chronic Suppurative Otitis Media ◽

Suppurative Otitis Media ◽

Methicillin Resistant

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Clinico-microbial profile in chronic suppurative otitis media and its management in tertiary care centre

International Journal of Otorhinolaryngology and Head and Neck Surgery ◽

10.18203/issn.2454-5929.ijohns20185301 ◽

2018 ◽

Vol 5 (1) ◽

pp. 135

Author(s):

Rashmi Ramashesh ◽

Samira Abdul Wajid ◽

Smitha Chandra

Keyword(s):

Staphylococcus Aureus ◽

Otitis Media ◽

Antibiotic Sensitivity ◽

Chronic Suppurative Otitis Media ◽

Health Concern ◽

Research Centre ◽

Suppurative Otitis Media ◽

Gram Negative ◽

Sensitivity Pattern ◽

Pseudomonas Species

Background: Chronic suppurative otitis media (CSOM) is an ever growing concern of preventable hearing loss and is a major health concern especially in tropical countries. Lack of cleanliness, overcrowding, poverty, malnutrition and medical ignorance are few of the contributing factors. The microbiological flora is constantly changing due to indiscriminate use of antibiotics with rapid change in the antibiotic sensitivity patterns. Hence, this study was undertaken to determine the epidemiological profile, bacteriological profile and the antibiotic sensitivity pattern which would help in establishing a protocol in management of CSOM patients in the hospital.Methods: A total of 100 patients of CSOM attending the ENT Outpatient Department of The Oxford medical College, Hospital and Research Centre were included in the study. The samples were immediately sent to the Microbiology laboratory for further processing according to standard procedure. Results: Out of 100 cases, 74 were active mucosal type and 26 were active squamous, with Pseudomonas species (33.6%) being the predominant organism. Staphylococcus aureus was the next common organism with MSSA (16.3%) and MRSA (12.5%), CONS were 6.7%. Among the 28 gram-negative bacilli, Klebsiella pneumoniae (7.7%), was followed by Escherichia coli (5.7%), and Proteus mirabilis (4.8%).Conclusions: Pseudomonas species and Staphylococcus aureus are the commonest organisms of CSOM. Ciprofloxacin can be used as a topical agent for treating gram-negative bacilli. Among systemic antibiotics- Linezolid, Amikacin and Imepenam remains the drug of choice.

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