Prevalence and antibiotic sensitivity of Staphylococcus aureus and Pseudomanas aeruginosa in middle ear fluids of chronic suppurative otitis media and chronic rhinosinusitis patients undergoing ear surgery

Background: Otitis media (OM) is a big menace in countries like India. Chronic suppurative otitis media (CSOM) is chronic inflammation of middle ear, which affects the tympanic membrane, middle ear mucosa and other middle ear structures characterised by the presence of persistent perforation or persistent mucoid or muco-purulent discharge for at least 8 weeks. Variety of organisms founds in patients suffering from chronic otitis media like, Pseudomonas, Staphylococcus aureus, Proteus mirabilis, Klebsiella pneumonia and Escherichia coli found. The pattern of organisms varies in different geographical areas. The objectives of the study were to isolate bacteria from patients with ear discharge, to find out antibiotic sensitivity of isolated organismsMethods: This is an observational cross sectional study done in the OPD department of otorhinolaryngology, at P.D.U medical college and hospital, Rajkot, for a period of six months from January 2017 to June 2017. A total number 200 patients of both gender between 5 to 74 years who visited the OPD department of otorhinolaryngology with chronic ear discharge taken for study. Results: Out of 200 patients of 5-74 years with chronic suppurative otitis media were examined and out of this Hundred and twelve (54%) of the patients were males with mean age of 33.44. Pseudomonas aeruginosa was the commonest identified organisms the sensitivity pattern highly favoured Pipercillin, Amikacin and Polymyxin B, Cefoparazone. Conclusions: Chronic otitis media is more prevalent in males than females. Commonest organisms found were Pseudomonas aeruginosa followed by Staphylococcus aureus. Piperacillin, Amikacin, Gentamicin, Polymyxin B and Cefoperazone were found most effective agents against Pseudomonas aeruginosa.

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Clinico-microbial profile in chronic suppurative otitis media and its management in tertiary care centre

International Journal of Otorhinolaryngology and Head and Neck Surgery ◽

10.18203/issn.2454-5929.ijohns20185301 ◽

2018 ◽

Vol 5 (1) ◽

pp. 135

Author(s):

Rashmi Ramashesh ◽

Samira Abdul Wajid ◽

Smitha Chandra

Keyword(s):

Staphylococcus Aureus ◽

Otitis Media ◽

Antibiotic Sensitivity ◽

Chronic Suppurative Otitis Media ◽

Health Concern ◽

Research Centre ◽

Suppurative Otitis Media ◽

Gram Negative ◽

Sensitivity Pattern ◽

Pseudomonas Species

Background: Chronic suppurative otitis media (CSOM) is an ever growing concern of preventable hearing loss and is a major health concern especially in tropical countries. Lack of cleanliness, overcrowding, poverty, malnutrition and medical ignorance are few of the contributing factors. The microbiological flora is constantly changing due to indiscriminate use of antibiotics with rapid change in the antibiotic sensitivity patterns. Hence, this study was undertaken to determine the epidemiological profile, bacteriological profile and the antibiotic sensitivity pattern which would help in establishing a protocol in management of CSOM patients in the hospital.Methods: A total of 100 patients of CSOM attending the ENT Outpatient Department of The Oxford medical College, Hospital and Research Centre were included in the study. The samples were immediately sent to the Microbiology laboratory for further processing according to standard procedure. Results: Out of 100 cases, 74 were active mucosal type and 26 were active squamous, with Pseudomonas species (33.6%) being the predominant organism. Staphylococcus aureus was the next common organism with MSSA (16.3%) and MRSA (12.5%), CONS were 6.7%. Among the 28 gram-negative bacilli, Klebsiella pneumoniae (7.7%), was followed by Escherichia coli (5.7%), and Proteus mirabilis (4.8%).Conclusions: Pseudomonas species and Staphylococcus aureus are the commonest organisms of CSOM. Ciprofloxacin can be used as a topical agent for treating gram-negative bacilli. Among systemic antibiotics- Linezolid, Amikacin and Imepenam remains the drug of choice.

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Reliability of conventional ear swabs in tubotympanic CSOM

The Journal of Laryngology & Otology ◽

10.1017/s0022215100112885 ◽

1990 ◽

Vol 104 (6) ◽

pp. 460-462 ◽

Cited By ~ 4

Author(s):

K. G. S. Raju ◽

P. Unnykrishnan ◽

R. C. Nayar ◽

S. Dutt ◽

R. Macaden

Keyword(s):

Staphylococcus Aureus ◽

Multidrug Resistance ◽

Otitis Media ◽

Middle Ear ◽

Single Agent ◽

Chronic Suppurative Otitis Media ◽

Mixed Infections ◽

External Ear ◽

Gram Positive ◽

Suppurative Otitis Media

AbstractConventional external ear swab cultures were compared with cultures obtained from the middle ear, in 25 cases of tubotympanic type (‘safe’), chronic suppurative otitis media. These cultures were identical in 22 of the 25 cases.Anaerobic organisms were isolated in only one case. Mixed infections of both gram-positive and gramnegative organisms were seen in nine cases (36 per cent).Staphylococcus aureus was the commonest single agent isolated (16 cases). Multidrug resistance was seen in 12 of these isolates.

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Emergence of antibiotic resistance in bacteria isolated from tubotympanic type of chronic suppurative otitis media in Chhattisgarh

International Journal of Otorhinolaryngology and Head and Neck Surgery ◽

10.18203/issn.2454-5929.ijohns20194948 ◽

2019 ◽

Vol 5 (6) ◽

pp. 1674

Author(s):

B. R. Singh ◽

Sagarika Pradhan ◽

R. Murthy ◽

Ekta Agrawal ◽

Rekha Barapatre ◽

...

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Otitis Media ◽

Antibiotic Sensitivity ◽

Chronic Suppurative Otitis Media ◽

Causative Organism ◽

Suppurative Otitis Media ◽

Major Health Problem ◽

Common Organism ◽

Life Threatening

Background: Chronic suppurative otitis media (CSOM) is a common chronic ear disease and a major health problem in developing countries causing deafness and life threatening complications like meningitis and brain abscess. Early and effective treatment based on the knowledge of causative organism and their antibiotic sensitivity is essential for immediate clinical recovery.Methods: Total of 152 clinically diagnosed patients with CSOM safe were enrolled in the study and two swab of the discharge ear were obtained from each patient under aseptic precaution. Microbiological study was done according to standard operative guidelines. Results: Out of 152 patients 93 male and 59 were female, majority of the patient seen below 30 year of age. Most common organism found was Staphylococcus aureus (43.2%) and Pseudomonas aeruginosa (27.9%). Staphylococcus aureus was sensitive to vancomycin (98%) and linezolide (100%) and Pseudomonas aeruginosa was found highly sensitive to Imipenem cilastin (84.85%) and Imipenem (66.7%).Conclusions: In our study gram positive Staphylococcus aureus (43.2%) and gram negative bacilli P. aeuroginosa (27.9%) was the most common organism was found and this P. aeruginosa were hospital acquired infections. We found out high resistant to Cefepime, ceftazidime, ciprofloxacin and levofloxacin and aztreonam.

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Follow up System in Middle Ear Surgery for Chronic Suppurative Otitis Media

Hearing Impairment ◽

10.1007/978-4-431-68397-1_76 ◽

2004 ◽

pp. 387-389

Author(s):

Helmi Abdullah

Keyword(s):

Otitis Media ◽

Middle Ear ◽

Chronic Suppurative Otitis Media ◽

Middle Ear Surgery ◽

Ear Surgery ◽

Suppurative Otitis Media

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Correlation between histomorphometric changes and the type of aerobic bacteria isolated in chronic suppurative otitis media

Vojnosanitetski pregled ◽

10.2298/vsp1101046z ◽

2011 ◽

Vol 68 (1) ◽

pp. 46-50

Author(s):

Emilija Zivkovic-Marinkov ◽

Milan Stankovic ◽

Dragan Mihailovic ◽

Mila Bojanovic

Keyword(s):

Staphylococcus Aureus ◽

Otitis Media ◽

Middle Ear ◽

Granulation Tissue ◽

Bacterial Flora ◽

Inflammatory Cells ◽

Chronic Suppurative Otitis Media ◽

Aerobic Bacteria ◽

Middle Ear Mucosa ◽

Suppurative Otitis Media

Background/Aim. Bacterial flora is a very important factor in pathogenesis of chronic suppurative otitis media (CSOM) and significantly influences the type and intensity of osteolytic process. There are few histomorphometric investigations of middle ear mucosa in chronic otitis. The aim of this study was to identify aerobic bacteria responsible for chronic suppurative otitis media as well as their association with histomorphometric changes of middle ear mucosa. Methods. A prospective study that comprised 153 patients treated in the Clinc for Ear, Thorat and Nose Diseases, Nis, was conducted. Bacteriologic analysis of diseased ear secretion was carried out in all patients. Intraoperatively removed granulation tissue was used for histomorphometry. The analysed parameters were: the number of inflammatory cells, as well as vascularization and vasodilatation. Results. The most frequently isolated aerobic bacteria from chronic suppurative otitis media were Staphylococcus aureus (29.02%), Pseudomonas aeruginosa (29.02%) and Proteus spp. (21.76%). There was no correlation between the type of pathologic process and the type of bacteria.The number of inflammatory cells in the granulation tissue in pure cultures of Staphylococcus aureus was 1,597.33 ? 549.45 and in Pseudomonas auriginosa cultures was 2,639 ? 648. Conclusion. This study showed that there is a statistically significant correlation between the number of inflammatory cells in the granulation tissue and the type of aerobic bacteria we isolated. The intensity of the infection in chronic suppurative otitis media depends on the type of the isolated bacteria, which emphasizes the importance of adequate preoperative antimicrobial therapy.

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Bacteriological Profile of Chronic Suppurative Otitis Media and Antibiotic Susceptibility in a Tertiary Care Hospital in Shanghai, China

Ear Nose & Throat Journal ◽

10.1177/0145561320923823 ◽

2020 ◽

pp. 014556132092382

Author(s):

Jianghong Xu ◽

Qiang Du ◽

Yilai Shu ◽

Jian Ji ◽

Chunfu Dai

Keyword(s):

Staphylococcus Aureus ◽

Otitis Media ◽

Middle Ear ◽

Antibiotic Susceptibility ◽

Tertiary Care Hospital ◽

Tertiary Care ◽

Chronic Suppurative Otitis Media ◽

Care Hospital ◽

Suppurative Otitis Media ◽

Bacteriological Profile

Objective: Chronic suppurative otitis media (CSOM) is mostly caused by bacterial infection of the middle ear and antibiotics are generally used empirically, which may lead to the emergence of resistant bacterial strains. The objective of the study is to assess the bacteriological profile of and evaluate the antibiotic susceptibility of strains isolated in a tertiary care hospital in Shanghai, China. Methods: This study included 289 individuals with clinical diagnosis of CSOM. Middle ear purulent discharge was obtained with sterile swabs and cultured for bacterial pathogens. The susceptibility of the isolated microorganisms to antibiotics was assessed by a microdilution method. Results: Bacterial pathogens were found in 223 (77.2%) of the 289 cases. A total of 236 strains were isolated. Staphylococcus aureus was the commonest bacteria (44.9%) followed by Pseudomonas aeruginosa (16.9%) and coagulase-negative Staphylococcus (8.5%). There were 18.9% methicillin-resistant S aureus (MRSA) among the obtained S aureus organisms. Multidrug-resistant P aeruginosa was found in 4 patients, making up 10% of all detected P aeruginosa. Staphylococcus aureus showed highest susceptibility to vancomycin (100%), then gentamicin (98.1%) and rifampicin (97.2%) and was most resistant to penicillin (61.3%) and erythromycin (50%). All isolated P aeruginosa showed susceptibility to piperacillin, piperacillin/tazobactam, and meropenem. High degree of resistance in P aeruginosa was observed toward levofloxacin (42.5%), ciprofloxacin (40%), and ceftriaxone (30%). Conclusion: The high prevalence of MRSA and fluoroquinolone-resistant P aeruginosa indicated cephalosporins and fluoroquinolone as primary empirical antibiotic drugs in CSOM to be cautiously used. In order to reduce the incidence of resistant strains and promote effective usage of antibiotics, all aural discharges should be cultured to determine antibacterial susceptibility patterns before treatment.

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Microbiology and antibiotic sensitivity of uncomplicated chronic suppurative otitis media at Dr. George Mukhari Academic Hospital, South Africa

International Journal of Otorhinolaryngology and Head and Neck Surgery ◽

10.18203/issn.2454-5929.ijohns20211177 ◽

2021 ◽

Vol 7 (4) ◽

pp. 565

Author(s):

Monica Kotu ◽

Ian Paul Olwoch

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Otitis Media ◽

Proteus Mirabilis ◽

Antibiotic Sensitivity ◽

Chronic Suppurative Otitis Media ◽

Older Children ◽

Suppurative Otitis Media ◽

Sensitivity Pattern ◽

Age Range

Background: The study was conducted to assess the type and frequency of isolation of different microorganisms in uncomplicated chronic suppurative otitis media (CSOM) and their antibiotic sensitivity in our institution.Methods: A total of 88 consecutive patients with unilateral or bilateral active, chronic suppurative otitis media attending outpatient department at DGMAH were included in the study after obtaining an informed consent. There were 55 males (62.5%) and 33 females (37.9%) with age range between 6 months and 76 years. Pus swabs were taken through the perforation site and from the promontory after ear mopping under direct vision. Results: Children less than 5 years were more affected (23.9%) than older children 5-10 years (13.6%) and 11-15 years (11.45%). One hundred and six microorganisms were isolated from analysis of cultures obtained from 72 patients. Seven cultures were negative (5.9%), 8 specimens were contaminated (6.7%) and 1 specimen was lost (1.1%). Pseudomonas aeruginosa (24.0%) was the most common isolate, followed by Staphylococcus aureus (17%) and Proteus mirabilis (10%). Drug sensitivity pattern showed that Piperacillin-tazobactam was effective against the majority of Pseudomonas aeruginosa isolates at 72%, followed by both Gentamicin and Ceftazidime at 64% and Ciprofloxacin at 48%. Staphylococcus aureus isolates were sensitive to Erythromycin (77%), Cloxacillin and Clindamycin at 72%. Proteus mirabilis was sensitive to Cefuroxime (91%), Co-amoxiclav (72.8%).Conclusions: Isolation rate and susceptibility patterns in CSOM, suggest a need for regular surveillance to monitor antimicrobial resistance and to guide antibacterial therapy. <h1> </h1>

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