Morphological and nuclear ribosomal DNA data support distinguishing two new species of Umbilicaria (Umbilicariaceae, Ascomycota) from Europe

2009 ◽  
Vol 41 (6) ◽  
pp. 631-648 ◽  
Author(s):  
Beata KRZEWICKA ◽  
Miguel A. GARCÍA ◽  
Steinar D. JOHANSEN ◽  
Leopoldo G. SANCHO ◽  
María P. MARTÍN
Keyword(s):  
New Species ◽  
Molecular Data ◽  
Nuclear Ribosomal Dna ◽  
Lsu Rdna ◽  
Group I Introns ◽  
New Taxon ◽  
Reticular Pattern ◽  
Group I ◽  
Clade C ◽  
Additional Support

AbstractUmbilicaria iberica and U. maculata are described as new to science on the basis of morphological and molecular data. Umbilicaria iberica is similar to U. polyphylla but differs in having a monophyllous thallus with a distinctive white reticular pattern over the umbilicus and actinodisc apothecia. Umbilicaria maculata is similar to U. cylindrica but this new species is distinguished by its grey-brown thallus with sparse marginal cilia and white stains on the upper surface especially in the marginal zone, as well as by sessile apothecia with one or a few sterile fissures. Nuclear ITS and LSU rDNA have been used as molecular markers. In the phylogenetic analysis U. polyphylla falls into two well supported clades (A and B), one of which corresponds to the morphotype that is described here as a new taxon. Specimens previously recognized as U.cylindrica fall into three well supported clades: clade C corresponds to the typical morphotype, clade D corresponds to morphotype II described here as a new taxon, and clade E is morphotype III which is recognized as U. cf. umbilicarioides. Typical group I introns have been found in position 1506 of the nuclear SSU rDNA of Umbilicaria; a consensus secondary structure of these introns is presented and we conclude that they represent an important and valuable molecular marker which gives additional support to the ITS and LSU sequence phylogeny obtained.

Inocybe cervenianensis (Agaricales, Inocybaceae), a new species in the I. flavoalbida clade from Italy

Phytotaxa ◽  
2021 ◽  
Vol 484 (2) ◽  
pp. 227-236
Author(s):  
FRANCESCO DOVANA ◽  
ENRICO BIZIO ◽  
MATTEO GARBELOTTO ◽  
GIULIANO FERISIN
Keyword(s):  
New Species ◽  
Natural Habitat ◽  
Molecular Data ◽  
Sensu Stricto ◽  
New Taxon ◽  
Microscopic Features ◽  
A New Species

The authors describe the macro- and microscopic features of Inocybe cervenianensis, a new taxon belonging to the genus Inocybe sensu stricto. The description is illustrated with photographs of the basidiomes in their natural habitat and photographs of the main microscopic features. Molecular data (nrITS and RPB2) support the recognition of this new species.

Molecular and morphological characterisation of Cryptaphelenchus baujardi n. sp. (Nematoda: Ektaphelenchinae), a new species based on two populations from Iran

Nematology ◽  
2019 ◽  
Vol 21 (1) ◽  
pp. 45-56 ◽  
Author(s):  
Farzad Aliramaji ◽  
Esmaeil Miraeiz ◽  
Ebrahim Pourjam ◽  
Ramin Heydari
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Lsu Rdna ◽  
New Taxon ◽  
Male Body ◽  
Northern Iran ◽  
Lateral Field ◽  
Rdna Sequences ◽  
Strong Resemblance ◽  
Two Populations

Summary Cryptaphelenchus baujardi n. sp. is described and illustrated based on two populations from Golestan province, northern Iran. The new species is characterised by female and male body length of 224 (190-261) and 215 (195-229) μm, respectively, offset cephalic region with a shallow constriction, delicate stylet 6.6 (6.0-7.0) μm long, four incisures in the lateral field, presence of a rudimentary post-uterine sac in the female gonoduct, elongate-conoid female tail ending in an acute to finely rounded terminus, male tail conical, male with seven caudal papillae and delicate spicules. The new species shows a strong resemblance to C. varicaudatus and C. iranicus, but has a sclerotised mass near the tip of the spicule and an indistinct spermatheca. Phylogenetic analyses based on both partial SSU and LSU rDNA sequences confirm its status as a new taxon.

Lecanicillium subprimulinum (Cordycipitaceae, Hypocreales), a novel species from Baoshan, Yunnan

Phytotaxa ◽  
2018 ◽  
Vol 348 (2) ◽  
pp. 99 ◽  
Author(s):  
SHIKE HUANG ◽  
SAJEEWA S.N. MAHARACHCHIKUMBURA ◽  
RAJESH JEEWON ◽  
D JAYARAMA BHAT ◽  
RUNGTIWA PHOOKAMSAK ◽  
...  
Keyword(s):  
New Species ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
Molecular Data ◽  
Its Sequence ◽  
New Taxon ◽  
Sexual Morph ◽  
The Family ◽  
Asexual Morphs ◽  
A New Species

In this paper, we report a new asexual-morph taxon belonging to the family Cordycipitaceae (Hypocreales), which is associated with a sexual morph of an ophioceras-like taxon on submerged wood collected from Baoshan, Yunnan, China. Morphologically, this new taxon is similar to known asexual morphs of Lecanicillium and characterized by conidiophores arising from hyaline hyphae, with gregarious, ellipsoid to ovoid, aseptate conidia. Phylogenetic analyses of a combined LSU, SSU, tef1-α and ITS sequence dataset positions our taxon in Cordycipitaceae and close to Lecanicillium sp. (CBS 639.85) and L. primulinum. Lecanicillium subprimulinum is introduced as a new species with support from molecular data.

A new species of Omolicna (Hemiptera: Auchenorrhyncha: Fulgoroidea: Derbidae) from coconut palm in Costa Rica and new country records for Omolicna brunnea and Omolicna triata

Zootaxa ◽  
2019 ◽  
Vol 4577 (3) ◽  
pp. 501 ◽  
Author(s):  
BRIAN W. BAHDER ◽  
CHARLES R. BARTLETT ◽  
EDWIN A. BARRANTES BARRANTES ◽  
MARCO A. ZUMBADO ECHAVARRIA ◽  
ALESSANDRA R. HUMPHRIES ◽  
...  
Keyword(s):  
New Species ◽  
Costa Rica ◽  
Ribosomal Rna ◽  
Sequence Data ◽  
Cocos Nucifera ◽  
Molecular Data ◽  
New Taxon ◽  
Additional Species ◽  
New Subgenus ◽  
A New Species

An ongoing survey for novel phytoplasmas and viruses that affect palms (Arecaceae) is being conducted in Costa Rica along with potential vectors. During that survey, a new species of derbid planthopper (Hemiptera: Fulgoroidea) in the genus Omolicna Fennah was detected from Heredia and Limón State and is here described as Omolicna xavieri sp. n. This new taxon was first found on declining coconut palms (Cocos nucifera) and subsequently on healthy coconut palms in Tortuguero National Park. Also, two additional species of Omolicna, O. brunnea and O. triata, were collected on coconut in the same habitat and represent new country records. There are no previously published records of Omolicna from Costa Rica. Sequence data for the cytochrome c oxidase subunit I (COI) and the 18S ribosomal RNA gene were obtained. In addition, novel 18S primers specific to auchenorrhynchan insects were developed and presented. The molecular data show high pairwise distances between O. xavieri sp. n. and other Omolicna species (24% to 31% and 10% using COI and 18S, respectively). Based on both morphological and sequence data, we erect and briefly describe the new subgenus Agoo for the new species to reflect the observed differences. A checklist of Omolicna species is presented. 

DESCRIPTION OF MUCOR PERNAMBUCOENSIS (MUCORALES, MUCOROMYCOTA), A NEW SPECIES ISOLATED FROM THE BRAZILIAN UPLAND RAINFOREST

Phytotaxa ◽  
2018 ◽  
Vol 350 (3) ◽  
pp. 274 ◽  
Author(s):  
CATARINA L. F. DE LIMA ◽  
DIOGO X. LIMA ◽  
CARLOS A. F. DE SOUZA ◽  
RAFAEL J. V. DE OLIVEIRA ◽  
INGRID B. CAVALCANTI ◽  
...  
Keyword(s):  
New Species ◽  
Molecular Data ◽  
Lsu Rdna ◽  
Rdna Sequences ◽  
A New Species

The Mucor genus mostly comprises saprobe specimens which are commonly isolated from herbivore dung, fruit and soil. Morphologically, Mucor specimens produce simple or branched sporangiophores without basal rhizoids and non-apophysate sporangia. During a survey of Mucorales in soil from an upland rainforest area in Pernambuco, Brazil, one specimen of Mucor was isolated and characterized based on morphological, physiological and molecular data (ITS1-5.8S-ITS2 and LSU rDNA sequences). The specimen is characterized by producing strongly sympodially branched sporangiophores and sporangiospores which can contain one or more granules at each end. The columellae are frequently globose or obovoid, some ovoid or cylindrical with a truncate base, rarely piriform.  It grew better at 25ºC, with no development at 35ºC. Based on the evidence of the analyzed datasets, a new species of Mucor is proposed.

A new Crocus L. (Iridaceae) species from SE Turkey, based on morphological and molecular data

Phytotaxa ◽  
2015 ◽  
Vol 239 (3) ◽  
pp. 223 ◽  
Author(s):  
Osman Erol ◽  
Doerte Harpke ◽  
Hasan Yıldırım
Keyword(s):  
Phylogenetic Analyses ◽  
Type Specimen ◽  
Morphological Characters ◽  
Molecular Data ◽  
Nuclear Ribosomal Dna ◽  
Phylogenetic Position ◽  
Full Description ◽  
New Taxon ◽  
Internal Transcribed Spacer Region ◽  
Se Turkey

Crocus musagecitii is described as a new species. Diagnostic morphological characters, a full description and detailed illustrations are provided on the basis of the type specimen and wild specimens. Morphologically, C. musagecitii is close to Crocus biflorus subsp. pseudonubigena. Crocus musagecitii differs from C. biflorus subsp. pseudonubigena by the lack of stripes or narrow purplish tongue on outside of outer tepals, wider tepals, and homogenously yellow anthers. In order to clarify the phylogenetic position of this species within the Crocus adamii species complex, we sequenced the internal transcribed spacer region (ITS: ITS1 + 5.8SrDNA + ITS2) and 5’ external transcribed spacer (ETS) of the nuclear ribosomal DNA (rDNA). A phylogenetic tree obtained by Bayesian phylogenetic inference is given. Phylogenetic analyses revealed that the new taxon is close to C. munzurensis. Crocus musagecitii differs from its phylogenetically closest relative C. munzurensis by the corm tunics (C. musagecitii: coriaceus; C. munzurensis: membranous), the number of leaves (C. musagecitii: up to 8; C. munzurensis: up to 4) and non-hairy leaf margins.

A new Gymnotus (Teleostei: Gymnotiformes: Gymnotidae) from the Pantanal Matogrossense of Brazil and adjacent drainages: continued documentation of a cryptic fauna

Zootaxa ◽  
2005 ◽  
Vol 933 (1) ◽  
pp. 1 ◽  
Author(s):  
FLORA M.C. FERNANDES ◽  
JAMES S. ALBERT ◽  
MARIA D.F.Z. DANIEL-SILVA ◽  
CARLOS E. LOPES ◽  
WILLIAM G.R. CRAMPTON ◽  
...  
Keyword(s):  
New Species ◽  
Lateral Line ◽  
Dna Amplification ◽  
Molecular Data ◽  
Body Cavity ◽  
The Body ◽  
New Taxon ◽  
Neotropical Fishes ◽  
Posterior Lateral Line ◽  
Floating Macrophytes

Here we describe a new species of Gymnotus, G. pantanal n. sp., from the Pantanal Matogrossense of Brazil, using morphological, cytogenetic, and molecular data. Specimens ascribed to the new species are also known from areas downstream in Paraguay, and from the adjacent Guaporé basin of Bolivia. The new species most closely resembles G. anguillaris in possessing an elongate body, slender profile, long body cavity, and shorter head than other congeners. The new species also resembles G. anguillaris in the presence of pale narrow bands restricted to the area below the lateral line on the anterior half of the body. The new taxon differs from G. anguillaris in possessing more narrowly set eyes, a wider and deeper head, a larger branchial opening, longer pectoral fins with more fin rays, and fewer pored posterior lateral-line scales. The new species inhabits rooted grasses and floating macrophytes in small creeks and along the banks of larger blackwater rivers. Populations are found syntoptically with G. inaequilabiatus and G. sylvius. Compared with these species, the new species exhibits a distinct combination of microsatellite DNA amplification patterns, and chromosomal and external features. These results confirm earlier studies showing the power of a multidisciplinary approach to characterizing the enormous and often cryptic diversity of Neotropical fishes.

Rhynchostegium occultum (Brachytheciaceae), a new species from relict forests of central Chile

Phytotaxa ◽  
2020 ◽  
Vol 453 (3) ◽  
pp. 199-217
Author(s):  
JUAN BERNARDO LARRAÍN ◽  
SANNA HUTTUNEN ◽  
ELENA IGNATOVA ◽  
MICHAEL IGNATOV
Keyword(s):  
New Species ◽  
Molecular Data ◽  
New Combination ◽  
New Taxon ◽  
Distribution Map ◽  
Central Chile ◽  
Recent Introduction ◽  
New Synonyms ◽  
Local Species ◽  
A New Species

We present a description of the new species Rhynchostegium occultum from central Chile, including illustrations, a distribution map, and preliminary molecular data that supports its taxonomic placement. The new taxon is restricted to coastal relict forests of central Chile. An overview of the Chilean taxa of Rhynchostegium, and a key for local species are also provided. The new combination Rhynchostegium corralense, and new synonyms for Rhynchostegium acanthophyllum and for Cratoneuropsis chilensis are proposed. Oxyrrhynchium hians is newly reported for Chile, apparently being a recent introduction. Lectotypes are selected for Hypnum corralense, Hypnum acanthophyllum, and for Rhynchostegiella acanthophylla var. robusta.

Allium gilanense, a new species of Allium sect. Codonoprasum (Amaryllidaceae) from Iran: evidence from morphological and molecular data

Phytotaxa ◽  
2020 ◽  
Vol 474 (3) ◽  
pp. 283-292
Author(s):  
ALI BAGHERI ◽  
FRANK R. BLATTNER ◽  
REINHARD M. FRITSCH
Keyword(s):  
New Species ◽  
Intergenic Spacer ◽  
Molecular Data ◽  
Internal Transcribed Spacers ◽  
Nuclear Ribosomal Dna ◽  
Morphological Data ◽  
Morphological Description ◽  
Allium Species ◽  
The North ◽  
North Of Iran

As a result of recent botanical expeditions to the north of Iran, we describe here a new endemic Allium species from Gilan province named Allium gilanense. Molecular and morphological data indicate that it belongs to Allium sect. Codonoprasum. We provide a morphological description, comparing Allium gilanense with the closest relative taxa A. lenkoranicum and A. paniculatum, the preliminary karyotype of the new species, and molecular phylogenetic data derived from the nuclear ribosomal DNA internal transcribed spacers (ITS) and the chloroplast intergenic spacer trnH-psbA. The chromosome number of the new species is 2n = 16 with 0-3 B chromosomes.

Description of Phasmarhabditis californica n. sp. and first report of P. papillosa (Nematoda: Rhabditidae) from invasive slugs in the USA

Nematology ◽  
2016 ◽  
Vol 18 (2) ◽  
pp. 175-193 ◽  
Author(s):  
Irma Tandingan De Ley ◽  
Oleksandr Holovachov ◽  
Rory J. Mc Donnell ◽  
Wim Bert ◽  
Timothy D. Paine ◽  
...  
Keyword(s):  
Phylogenetic Analyses ◽  
Molecular Data ◽  
Egg Laying ◽  
The Body ◽  
Lsu Rdna ◽  
New Taxon ◽  
Sequence Alignments ◽  
Single Male ◽  
The Usa ◽  
Basal Bulb

A new species ofPhasmarhabditisand the known speciesP. papillosawere isolated from cadavers of invasive slugs in California.Phasmarhabditis papillosais the type of the genus and has not previously been reported from the Americas. Both species are characterised based on morphology, morphometrics and molecular data. Molecular phylogenies were inferred from concatenated DNA sequence alignments of nearly complete SSU and the D2-D3 domains of the LSU rDNA.Phasmarhabditis californican. sp. is characterised by a robust body, mature egg-laying specimens almost spindle-shaped when relaxed, 1.5 (1.3-1.8) mm long, tapering to a bluntly rounded anterior end with stoma about as long as lip region diam., six inner labial papillae and four outer cephalic papillae, pharynx with rounded to pyriform basal bulb, vulva located halfway along the body, hermaphroditic, didelphic, amphidelphic, and short, conoid tail constricted at one-third its length with prominent phasmids. Not a single male was found among five strains.Phasmarhabditis papillosais gonochoristic and has a longer isthmus, pyriform basal bulb and longer, dome-shaped spicate female tail constricted halfway along its length. Sequence analysis revealed unambiguous autapomorphies forP. papillosaandP. californican. sp. Phylogenetic analyses placed these two species in a monophyletic clade comprisingPhasmarhabditisspecies and other gastropod-parasitic taxa. Morphology, genetic distance, reproductive strategy and nucleotide autapomorphies support the new taxon.

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