European Taxa of Saxicolous Lecidella Containing Chloroxanthones:Identification of Patterns Using Thin Layer Chromatography

1992 ◽  
Vol 24 (4) ◽  
pp. 383-397 ◽  
Author(s):  
Ch. Leuckert ◽  
J.-G. Knoph
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Glacial Acetic Acid ◽  
Uv Light ◽  
Rf Values ◽  
Layer Chromatography ◽  
Spray Reagents

AbstractA method using TLC to recognize compound patterns consisting almost entirely of chloroxanthones in European saxicolous species of Lecidella is described. These are necessary for diagnosis of the taxa studied and of their chemotypes. Two solvents with complementary separatingqualities were required: C, toluene-glacial acetic acid (100:15); J, dichloromethane-acetone (4:1). Although the Rf values in solvent J strongly depend on the concentration of the substances, it is very suitable for identification because the xanthones in this system reveal distinct and characteristic fluorescence colours in UV light, simplifying interpretation enormously. No spray reagents are used. Identifications are made by co-chromatography with authentic lichencompounds

Identifikasi Senyawa Jamu Pegal Linu yang Beredar di Kabupaten Bantul dengan Metode Kromatografi Lapis Tipis

2019 ◽  
Vol 14 (2) ◽  
pp. 80
Author(s):  
Furijika Fitriana Mosy ◽  
Kuswandani Kuswandani
Keyword(s):  
Acetic Acid ◽  
Herbal Medicine ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
Random Sampling ◽  
Uv Light ◽  
Physicochemical Method ◽  
Layer Chromatography

Background: Traditional herbal is not permitted to contain chemicals drug. It is possible allowed such as paracetamol and phenylbutazone.Objective: This study is aimed to identify the compounds of paracetamol and phenylbutazone in the traditional herbal of Jamu Pegal Linu (Herbal Medicine).Methods: This type of research was descriptive and the sample was determined by random sampling. The method used Thin Layer Chromatography (TLC) which is a physicochemical method. There were eight samples were extracted by the soxhletation method until a thick extract was obtained to be spotted in the quite phase of TLC silica gel F254. The motion phase used for the analysis of paracetamol was chloroform: acetone: toluene (65:25:10) and the motion phase for phenylbutazone analysis was benzene: chloroform: 96% acetic acid (50:40:10). Spots detection was done by observing under UV light of 254 nm and the spots that appeared were calculated for Rf value and compared with the standard Rf value of paracetamol and phenylbutazone.Results: The results obtained in this study were positive E samples containing paracetamol and phenylbutazone with Rf value of sample 0.57 and a standard Rf of paracetamol 0.57 and an Rf value of sample 0.82 and a standard Rf of phenylbutazone 0.86. The positive G sample contained paracetamol with a sample Rf value of 0.61 and a standard Rf value of paracetamol 0.68.Conclusion: From the eight samples of ‘Jamu Pegal Linu’, two of them were positive containing chemical drugs paracetamol and phenylbutazone.Keywords: Jamu Pegal Linu (Herbal Medicine), Paracetamol, Phenylbutazone, TLC

Separation of dl-2-(3-Phenoxyphenyl) propionic Acid (Fenoprofen) From Its Intermediates by Thin Layer Chromatography

1973 ◽  
Vol 56 (3) ◽  
pp. 656-658
Author(s):  
Rafik H Bishara
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Lower Limit ◽  
Raw Material ◽  
Chemical Constitution ◽  
Tlc Plates ◽  
Rf Values ◽  
Synthetic Intermediates ◽  
Layer Chromatography

Abstract Fenoprofen and its synthetic intermediates are resolved on silica gel F254 TLC plates developed with chloroform-acetic acid (98+2). The interrelationship between the chemical constitution and the Rf values is discussed. The lower limit of detecting the intermediates in Fenoprofen raw material is 1%.

scholarly journals Identifikasi Senyawa Metabolit Sekunder Ekstrak Etanol Kulit Buah Pisang Kepok (Musa paradisiaca L) dengan Metode KLT

2019 ◽  
Vol 5 (2) ◽  
pp. 125
Author(s):  
Hasma Hasma ◽  
Winda Winda
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Uv Light ◽  
Banana Peel ◽  
Banana Plant ◽  
Chemical Reagents ◽  
Musa Paradisiaca ◽  
Rf Values ◽  
Layer Chromatography ◽  
Peel Extract

Indonesia is a country that is rich in abundant natural plants by having various types of plants which have medicinal properties and are used by some people as traditional medicines. One use of traditional medicine used by the community is the banana plant. So far the community only uses bananas, limited to the use of their fruit, then the banana peel after consumption is only disposed of as waste that is not used optimally by the community. This study aims to identify the chemical compounds found in the skin of Kepok Banana (Musa paradisiaca L) taken from the pioneer road in the city of Makassar. This identification is carried out using the Thin Layer Chromatography (TLC) method and chemical reagents. Kepok banana peel extract (Musa paradisiaca L.) is made using maceration method, because the method is not only simple, the equipment used is simple, easy to work on, can also avoid damage to compound components due to heat. The yield of Rendamen obtained was 12.06%. Identification was done using Silica Thin Layer Chromatography GF254 with N-hexane eluent: ethyl acetate (6: 4) eluents obtained 5 stains on 366 nm UV light, namely alkaloids with orange blotches with Rf value (0.13), saponins with blemishes. purple Rf value (0.92), flavonoids with yellow spots Rf values ​​(0.33) and tannins with blackish orange patches Rf values ​​(0.65). Based on testing using chemical reagents obtained positive results saponins, flavonoids, tannins and alkaloids. The conclusion of the study showed that the Kepok banana peel extract taken on the pioneering road of Makassar city contained alkaloids, saponins, flavonoids and tannins.

OPTIMIZATION OF THIN LAYER CHROMATOGRAPHY METHODS FOR SEPARATION AND IDENTIFICATION OF ANTIDEPRESSANTS IN THEIR MIXTURE

2014 ◽  
Vol 21 (1) ◽  
pp. 11-15
Author(s):  
Daiva Kazlauskienė ◽  
Guoda Kiliuvienė ◽  
Palma Nenortienė ◽  
Giedrė Kasparavičienė ◽  
Ieva Matukaitytė
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Solvent System ◽  
Ammonia Solution ◽  
Relative Standard ◽  
Rf Values ◽  
Solvent Systems ◽  
Paroxetine Hydrochloride ◽  
Fluvoxamine Maleate ◽  
Layer Chromatography

By conducting the toxicological analysis it is meaningful to determine the analytical system that could identify simultaneously several medicinal preparations quickly and precisely. The purpose of this work was to create and validate the method of thin-layer chromatography that would be suitable to separate the components of antidepressant mixture (amitriptyline hydrochloride, paroxetine hydrochloride, sertraline hydrochloride, fluvoxamine maleate and buspirone hydrochloride) and to identify them. The system was validated with regard to the sensitivity, repetition of data, resistance and particularity. The solvent systems with potential of high separation of components in their mixture were created: acetonitrile, methanol, ammonia solution 25 percent (85:10:5); acetonitrile, methanol, ammonia solution 25 percent (75:20:5); dichlormethane, 1,4-dioxane, ammonia solution 25 percent (50:45:5); dichlormethane, 1,4-dioxane, ammonia solution 25 percent (42:55:3); trichlormethane, 1,4-dioxane, ammonia solution 25 percent (25:70:5); trichlormethane, 1,4-dioxane, ammonia solution 25 percent (60:36:4). One of the most suitable solvent systems for separation of the analyzed mixture (sertraline, amitriptyline, paroxetine, buspirone, fluvoxamine) was determined – acetonitrile, methanol, ammonia solution 25 percent (85:10:5). When this solvent system was used, the average Rf values of the analyzed compounds differed the most. Validation was conducted – the relative standard deviation (RSD, percent) of the average Rf value of the analyzed compounds varied from 0,6 to 1,8 percent and did not exceed the permissible error of 5 percent. The sensitivity of methodology was determined by assessing the intensity of the mixture’s spots on the chromatographic plate. The detection limit of buspirone was 0,0012 µg; sertraline – 0,0008 µg; amitriptyline – 0,0004 µg; fluvoxamine – 0,0004 µg; paroxetine – 0,0008 µg. The resistance of results to the changed conditions – it was determined that when the amounts of the solvents acetonitrile and methanol were increased or decreased to two milliliters, the average Rf values of the analyzed compounds did not change statistically significantly

scholarly journals A validated quantification of triclosan in toothpaste using high-performance thin-layer chromatography and a 48-bit fatbed scanner

2021 ◽  
Author(s):  
Barbara Anders ◽  
Sabrina Doll ◽  
Bernd Spangenberg
Keyword(s):  
Acetic Acid ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Detection System ◽  
Flatbed Scanner ◽  
Butyl Ether ◽  
Quantification Method ◽  
Direct Measurements ◽  
Layer Chromatography

AbstractWe present a densitometric quantification method for triclosan in toothpaste, separated by high-performance thin-layer chromatography (HPTLC) and using a 48-bit flatbed scanner as the detection system. The sample was band-wise applied to HPTLC plates (10 × 20 cm), with fluorescent dye, Merck, Germany (1.05554). The plates were developed in a vertical developing chamber with 20 min of chamber saturation over 70 mm, using n-heptane–methyl tert-butyl ether–acetic acid (92:8:0.1, V/V) as solvent. The RF value of triclosan is hRF = 22.4, and quantification is based on direct measurements using an inexpensive 48-bit flatbed scanner for color measurements (in red, green, and blue) after plate staining with 2,6-dichloroquinone-4-chloroimide (Gibbs' reagent). Evaluation of the red channel makes the measurements of triclosan very specific. For linearization, an extended Kubelka–Munk expression was used for data transformation. The range of linearity covers more than two orders of magnitude and is between 91 and 1000 ng. The separation method is inexpensive, fast and reliable.

DEVELOPMENT OF HPTLC METHOD FOR THE ESTIMATION OF ONDANSETRON AND RANITIDINE IN COMBINED DOSAGE FORM

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (12) ◽  
pp. 42-48
Author(s):  
P. J. Patel ◽  
◽  
D. A Shah ◽  
F. A. Mehta ◽  
U. K. Chhalotiya
Keyword(s):  
Thin Layer ◽  
Stationary Phase ◽  
Thin Layer Chromatography ◽  
Silica Gel ◽  
High Performance ◽  
Dosage Form ◽  
Solvent System ◽  
Rf Values ◽  
Layer Chromatography ◽  
Hptlc Method

A simple, sensitive and precise high performance thin layer chromatographic (HPTLC)method has been developed for the estimation of ondansetron (OND) and ranitidine (RAN) in combination. The method was employed on thin layer chromatography (TLC) and aluminium plates were precoated with silica gel 60 F254 as the stationary phase, while the solvent system was methanol. The Rf values were observed to be 0.5 ± 0.02, and 0.3 ± 0.02 for OND and RAN, respectively. The separated spots were densitometrically analyzed in absorbance mode at 299 nm. This method was linear in the range of 25-300 ng/band for OND and 50-600 ng/band for RAN. The limits of detection for OND and RAN were found to be 3.47 and 1.83 ng/band, respectively. The limits of quantification for OND and RAN were found to be 10.53 and 5.55 ng/band, respectively. The proposed method was validated with respect to linearity, accuracy, precision and robustness. The method was successfully applied to the estimation of OND and RAN in combined dosage form.

scholarly journals High Performance Thin Layer Chromatography Fingerprinting Analysis of Piper betle L. Leaves

2021 ◽  
pp. 8-15
Author(s):  
Ramdas N. Kale ◽  
Ravindra Y. Patil
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Methanolic Extract ◽  
Chemical Constituents ◽  
Piper Betle ◽  
Soxhlet Apparatus ◽  
Rf Values ◽  
Pharmacologically Active ◽  
Layer Chromatography

Introduction: Many modern medicines used today based on plants and plant products. Piper betle is generally known as the betle vine, it is an important medicinal and recreational plant. High performance thin layer chromatography (HPTLC) is an advanced powerful analytical method with more separation power, high performance and superior reproducibility than classic thin layer chromatography (TLC). A chromatographic fingerprint of a plant extract is a chromatographic pattern of some common chemical constituents of pharmacologically active and/or chemical characteristics. Chromatographic fingerprints are useful in authentication and identification of plant. Objectives:  Objectives of present research was to establish HPTLC fingerprinting of methanolic extract of Piper betle L. leaves. Materials and Methods: Methanolic extract of Piper betle leaves was prepared using soxhlet apparatus. HPTLC studies were performed using a CAMAG HPTLC system equipped with automatic TLC sampler-4 (ATS 4), TLC scanner 4, and vision CATS 3.0 software. Results: The study revealed the presence of alkaloids with Rf value 0.65, flavonoids with Rf values 0.19, 0.29, 0.72, 0.95., and phenolic compound with Rf value 0.7. Conclusion: The HPTLC fingerprinting profile developed for the methanolic extract of Piper betle L. leaves will help in proper identification of the plant.Piper betle

scholarly journals Profile of Thin-Layer Chromatography and UV-Vis Spectrophotometry of Akar Kuning Stem Extract (Arcangelisia flava)

2018 ◽  
Vol 1 (2) ◽  
pp. 72-76 ◽  
Author(s):  
Mohammad Rizki Fadhil Pratama ◽  
Suratno Suratno ◽  
Evi Mulyani
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Ethanol Extract ◽  
Retention Factor ◽  
Polar Solvents ◽  
Central Kalimantan ◽  
Stem Extract ◽  
Rf Values ◽  
Layer Chromatography ◽  
Chloroform Methanol

This study aims to obtain the profile of Thin-Layer Chromatography (TLC) and Ultraviolet-Visible (UV-Vis) spectrophotometry from ethanol extract of akar kuning stems (Arcangelisia flava) from Central Kalimantan. The TLC method is used with the orientation phase of the combination of polar-non-polar solvents resulting from orientation, while ethanol is used as the solvent for UV-Vis spectrophotometers. TLC results showed the formation of 3 stains on a combination of polar solvents chloroform : methanol : water while in a non-polar solvent combination n-hexane : ethyl acetate did not show any stains. Comparison of retention factor (Rf) values show the best combination of polar solvents to separate stains at a ratio of 5 : 2 : 1, respectively. Separation in 2-dimensional TLC with polar solvents showed a similar pattern with 1-dimensional separation in the form of 3 stains. UV-Vis spectrophotometer results showed 4 main peaks with wavelength 227.2; 267.4; 345.2; and 425.3 nm, respectively. The profile of the peak formed is very similar to that shown by berberine, one of the main metabolites of akar kuning. TLC and UV-Vis spectrophotometers profiles obtained are expected to support further research using akar kuning stems, especially those from Central Kalimantan.

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