Large intestinal resection induces changes in human colon: study of H+/oligopeptide cotransporter 1 (PepT1), Na+/H+ exchangers 3 and 2 (NHE 3 and NHE 2) and intestinal microbiota

Introduction. The metabolic activity of the intestinal microbiota is an important indicator of the functioning of the digestive tract, one of the main functions of which is the absorption of nutrients.The aim of the study was to analyze the content of short chain fatty acids in feces and assess their relationship with the indicators of physical development in children 3 years of age.Materials and methods. A prospective cohort study was conducted in 89 children 3rd year of life. The 1st observation group included children of the I and II health groups, the children of the 2nd group underwent surgical treatment on the intestine in the first months of life, the children of the3rd group are with atopic dermatitis. FD assessment was carried out in accordance with WHO recommendations (2017) the spectrum of FGC in feces was determined by gas-liquid chromatography.Results. It was determined the features of the rates of FD in healthy children, children with a history of intestinal resection and children with atopy in relation to the indicators of metabolic activity of the intestinal microbiota. The percentile distribution of the values of FGC in feces and their comparative assessment in the observation groups are given.Discussion. The content of FGM in feces has differences depending on the health status of children of the 3rd year of life. Different level of FGC shows a close relationship with the indicators of FD. Conclusions. A decrease in growth rates (body length SD: from -1 to -2) was revealed at low values (<25%) of the amount of acids, with an increase in propionate (25%) in feces. Reduced nutrition (body weight SD: from -1 to -2) it was associated with low values of the sum of isoacids and AI(-) (<25%); by 3rd year of life, the risk of low nutrition decreased in 4 times with high values (75%) of the sum of acids in feces (OR=4.33[1.09-17.71]). The multidirectionality of the content of fecal matter in young children and their influence on the indicators of FD emphasizes the versatility of the functions of the intestinal microbiota, which has a local and systemic effect on the human body.

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Metabolism of the intestinal microbiota in children of the first year of life with intestinal pathology and its surgical correction as a potential marker of biological age

BIO Web of Conferences ◽

10.1051/bioconf/20202202024 ◽

2020 ◽

Vol 22 ◽

pp. 02024

Author(s):

Galina Fedotova ◽

Irina Vakhlova ◽

Lyubov Boronina

Keyword(s):

Intestinal Microbiota ◽

Metabolic Activity ◽

Intestinal Resection ◽

Short Chain Fatty Acids ◽

First Year ◽

Fecal Matter ◽

Potential Marker ◽

Intestinal Pathology ◽

First Year Of Life ◽

Liquid Chromatographic

The article presents data on the study of the metabolic activity of the intestinal microbiota in children of the first year of life. The study was performed by gas-liquid chromatographic analysis with the determination of short-chain fatty acids (SCFA) in fecal matter, which reflects the microbiota metabolic activity. The content of fecal matter was determined, reflecting the activity of aerobic microbiota-acetic acid (C2); anaerobic microbiota – propionic (C3), butyric (C4) acids; the sum of acids (E); anaerobic index (AI), which is an integral indicator of the intestinal environment. It was found that despite surgical intervention in the intestine in the first months of life, the metabolic activity of microbiota in children with intestinal resection remains stable throughout the first year of life.

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Ultrastructural Study of a differentiating human colon carcinoma cell line

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100158583 ◽

1990 ◽

Vol 48 (3) ◽

pp. 208-209

Author(s):

Sylvie Polak-Charcon ◽

Mehrdad Hekmati ◽

Yehuda Ben Shaul

Keyword(s):

Cell Line ◽

Morphological Changes ◽

Secretory Granules ◽

Human Colon ◽

Cell Types ◽

Culture Conditions ◽

Morphological Evidence ◽

Human Colon Carcinoma Cell ◽

Colon Cell

The epithelium of normal human colon mucosa “in vivo” exhibits a gradual pattern of differentiation as undifferentiated stem cells from the base of the crypt of “lieberkuhn” rapidly divide, differentiate and migrate toward the free surface. The major differentiated cell type of the intestine observed are: absorptive cells displaying brush border, goblet cells containing mucous granules, Paneth and endocrine cells containing dense secretory granules. These different cell types are also found in the intestine of the 13-14 week old embryo.We present here morphological evidence showing that HT29, an adenocarcinoma of the human colon cell line, can differentiate into various cell types by changing the growth and culture conditions and mimic morphological changes found during development of the intestine in the human embryo.HT29 cells grown in tissue-culture dishes in DMEM and 10% FCS form at late confluence a multilayer of morphologically undifferentiated cell culture covered with irregular microvilli, and devoid of tight junctions (Figs 1-3).

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