A comparative analysis of various developmental stages of Schistosoma mansoni with respect to their protein composition

Parasitology ◽  
1977 ◽  
Vol 75 (3) ◽  
pp. 339-343 ◽  
Author(s):  
Andreas Ruppel ◽  
Donato Cioli
Keyword(s):  
Comparative Analysis ◽  
Schistosoma Mansoni ◽  
Sodium Dodecyl Sulphate ◽  
Developmental Stages ◽  
Sodium Dodecyl ◽  
Protein Composition ◽  
Protein Patterns ◽  
High Degree ◽  
Slab Gels

SummaryThe protein composition of Schistosoma mansoni was analysed by electrophoresis in polyacrylamide slab gels in the presence of sodium dodecyl sulphate. A high degree of identity existed between protein patterns obtained from immature and adult parasites of the two sexes. Some stage-and sex-specific components were identified and their possible origin discussed.

Protein composition of various developmental stages of Dipetalonema viteae (Filarioidea)

1982 ◽  
Vol 56 (3) ◽  
pp. 251-255 ◽  
Author(s):  
A. Prüsse ◽  
H. J. Diesfeld ◽  
S. Vollmer
Keyword(s):  
Adult Male ◽  
Developmental Stages ◽  
Protein Composition ◽  
Total Proteins ◽  
Sodium Dodecylsulphate ◽  
Male And Female ◽  
Third Stage ◽  
Dipetalonema Viteae ◽  
Slab Gels

ABSTRACTThe protein composition of various developmental stages of Dipetalonema viteae was analysed on polyacrylamide slab gels in the presence of sodium-dodecylsulphate. When the total proteins of adult male and female parasites, microfilariae, eggs, and third-stage larvae were compared, apparent qualitative similarities between mature and immature filariae were observed. However, several stage specific components were also identified.

Analysis of two-dimensional protein patterns from developmental stages of the potato cyst-nematode, Globodera rostochiensis

Parasitology ◽  
1992 ◽  
Vol 105 (3) ◽  
pp. 461-474 ◽  
Author(s):  
J. M. De Boer ◽  
H. A. Overmars ◽  
J. Bakker ◽  
F. J. Gommers
Keyword(s):  
Developmental Stages ◽  
Polyacrylamide Gel Electrophoresis ◽  
Protein Composition ◽  
Globodera Rostochiensis ◽  
Cyst Nematode ◽  
Potato Cyst Nematode ◽  
Two Dimensional ◽  
Protein Patterns ◽  
Silver Stain ◽  
Molecular Weights

SUMMARYTwo-dimensional polyacrylamide gel electrophoresis was used to examine the differences in total protein composition between two motile stages and two sedentary stages of the potato cyst-nematode, Globodera rostochiensis. Using a sensitive silver stain, 542 reproducible protein spots were distinguished. A list of these spots is presented, showing their apparent molecular weights, estimated isoelectric points, and occurrences in the different developmental stages. When the protein patterns were compared, 401 spots were found to change their presence or size in one or more of the four developmental stages. It is therefore estimated that during the post-embryonic development of G. rostochiensis, 74% of the polypeptides undergo modulation of their synthesis, or are affected by protein degradation or modification. In the motile stages several abundant proteins were present, which disappeared or decreased in concentration in the sedentary stages. Some of these proteins are presumably muscle proteins, and their modulation may illustrate the degeneration of body-wall musculature in the sedentary stages. It is concluded that the potato cyst-nematode has a very dynamic protein metabolism.

scholarly journals Isolation and protein composition of gap junctions from rabbit hearts

1982 ◽  
Vol 205 (1) ◽  
pp. 189-194 ◽  
Author(s):  
C K Manjunath ◽  
G E Goings ◽  
E Page
Keyword(s):  
Gap Junctions ◽  
Sodium Dodecyl Sulphate ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Protein Composition ◽  
Polyacrylamide Gel ◽  
Major Protein ◽  
Electron Microscopic ◽  
Gap Junctional

We have modified a method for isolating gap-junctional membrane from mouse hearts [Kensler & Goodenough (1980) J. Cell Biol. 86, 755-764] to isolate gap junctions of comparable purity from rabbit hearts more rapidly, with better yield, and without resort to non-ionic detergents. Purification was monitored by electron microscopy of thin-sectioned membrane pellets and by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis. Gap junctions were obtained as vesicles whose mean surface area approximated that of junctions in intact myocardial cells. About 10-20% of the vesicles were ferritin-impermeable. Approx. 125 micrograms of membrane protein was obtained per 8 g of rabbit heart. Sodium dodecyl sulphate/polyacrylamide-gel electrophoresis of purified gap junctions showed five major protein bands of mol.wts. 46 000, 44 000, 33 000, 30 000 and 28 500 that co-purified with the junctions. This protein composition was nearly identical with that published for gap junctions of mouse hearts, and differed markedly from the protein composition of gap junctions from non-excitable cells (lens and liver). The constancy of junctional protein composition between hearts of two different species and its non-identity with that from liver and lens suggest that, although gap-junctional structure in mammalian tissues seems to be remarkably similar by electron-microscopic techniques, junctional-channel protein composition actually varies from tissue to tissue and may be adapted to the permeability requirements of the tissue.

Cyst Membrane Protein Composition as a Discriminant Character in the Genus Artemia. (International Study on Artemia LV)

1997 ◽  
Vol 77 (1) ◽  
pp. 265-268 ◽  
Author(s):  
T.J. Abatzopoulos ◽  
G.V. Triantaphyllidis ◽  
J.A. Beardmore ◽  
P. Sorgeloos
Keyword(s):  
Membrane Protein ◽  
Sodium Dodecyl Sulphate ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Protein Composition ◽  
International Study ◽  
Fibrous Layer ◽  
Cuticular Membrane ◽  
Cyst Membrane

Isolated embryonic membranes (i.e. the outer cuticular membrane, the fibrous layer, the inner cuticular membrane and the hatching membrane) and homogenates of incubated, decapsulated cysts of 16 populations from five Artemia species (Crustacea, Anostraca) were examined by sodium dodecyl-sulphate polyacrylamide gel electrophoresis. Characteristic differences in major polypeptides between 45 and 205 kD were seen. These differences might be used as markers for identification of different Artemia species. The validity of this approach in characterizing Artemia populations is discussed.

Biosynthesis and characterization of heat shock proteins in Chironomus tentans salivary glands

1981 ◽  
Vol 59 (1) ◽  
pp. 67-73 ◽  
Author(s):  
R. M. Tanguay ◽  
M. Vincent
Keyword(s):  
Heat Shock ◽  
Salivary Glands ◽  
Sodium Dodecyl ◽  
Kinetic Studies ◽  
Chironomus Tentans ◽  
Protein Patterns ◽  
Shock Proteins ◽  
Slab Gels ◽  
Induced Proteins

The protein synthesis response of Chironomus tentans salivary glands to a heat shock was analyzed by means of [3H]leucine pulse labeling, sodium dodecyl sulfate – polyacrylamide slab gels, and fluorography. Seven new cytoplasmic polypeptides with relative masses (Mr) of 90 000), 76 000, 73 000, 68 000, 28 000, 25 000, and 22 000 are synthesized in response to a39 °C heat shock (HS) while the synthesis of most of the normal proteins is reduced. The major HS-induced protein is the 68 000 species. The length of the heat treatment does not modify markedly the pattern of induced proteins but the temperature has an effect: while the responses at 37 and 39 °C are similar, two HS-induced polypeptides (Mr 90 000 and 76 000) are not seen after a treatment at 41 °C. Kinetic studies of the response show an asynchrony in the appearance of the various HS proteins indicating that their individual rates of synthesis differ or that their induction is not fully coordinated. No significant differences were found between the protein patterns of a mitochondria-rich cytoplasmic zone obtained by microdissection and the whole cytoplasm, but two heat-induced proteins are present in the microdissected nuclei: a 68 000 polypeptide, comigrating with the major cytoplasmic one, and a 34 000 protein, almost exclusively seen in the nucleus.

scholarly journals The proteins in the Z line of insect flight muscle

1977 ◽  
Vol 161 (2) ◽  
pp. 399-403 ◽  
Author(s):  
B Bullard ◽  
G M Sainsbury
Keyword(s):  
Ionic Strength ◽  
Sodium Dodecyl Sulphate ◽  
Gel Electrophoresis ◽  
Flight Muscle ◽  
Insect Flight ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Protein Composition ◽  
High Ionic Strength ◽  
Insect Flight Muscle

Z discs were isolated from Lethocerus flight muscle by removing the contractile proteins from myofibrils with a solution of high ionic strength. The protein composition of the Z discs was analysed by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis; the major proteins were alpha-actinin, actin and tropomyosin. Z lines were selectively removed from intact myofibrils by digestion with crude lipase and chymotrypsin, but not by purified lipase.

A comparative analysis of various parts of Ascaris suum with respect to their protein composition

1981 ◽  
Vol 19 (1) ◽  
pp. 1
Author(s):  
Won Young Choi ◽  
Young Kwan Jin ◽  
Ok Ran Lee ◽  
Woon Gyu Kim
Keyword(s):  
Comparative Analysis ◽  
Ascaris Suum ◽  
Protein Composition

Identifikasi Daging Tikus Pada Produk Baso Dengan Metode Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE)

2018 ◽  
Vol 26 (2) ◽  
pp. 058
Author(s):  
Anna P. Roswiem ◽  
Triayu Septiani
Keyword(s):  
Sodium Dodecyl Sulphate ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Polyacrylamide Gel ◽  
Sds Page

<em>Bahan<strong> </strong>baku untuk membuat baso adalah daging hewan, pada umumnya dari daging sapi, ayam, ikan dan babi. Di beberapa daerah di Indonesia terjadi kasus baso tikus. Tujuan penelitian ini adalah menguji ada tidaknya kandungan daging tikus pada produk baso yang dijual di pasar Cempaka Putih-Kecamatan Kramat Jakarta Pusat dan di pedagang baso atau mie baso di sekitar kampus Universitas YARSI Jakarta. Daging adalah protein salah satu metode untuk mengidentifikasi protein adalah metode Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE).<strong> </strong>Hasil penelitian menunjukkan bahwa dari 6 sampel baso terindikasi ada 2 sampel baso dengan nomor 1 dan 5 yang dibuat dari campuran daging sapi dan tikus; ada 1 sampel baso dengan nomor 6 yang terbuat dari daging tikus; dan 2 sampel baso dengan nomor 2 dan 3 yang terbuat dari campuran sapi  dan babi, dan hanya 1 sampel baso dengan nomor sampel 4 yang benar-benar terbuat dari daging sapi.</em>

Ultrasound Effect on Molecules of Sodium Dodecyl Sulphate as Systems of Nanoparticles

2018 ◽  
Vol 10 (6) ◽  
pp. 06013-1-06013-5 ◽  
Author(s):  
I. G. Vorobiova ◽  
◽  
Yu. A. Mirgorod ◽  
A. S. Chekadanov ◽  
◽  
...  
Keyword(s):  
Sodium Dodecyl Sulphate ◽  
Sodium Dodecyl ◽  
Ultrasound Effect
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