A comparative analysis of various parts of Ascaris suum with respect to their protein composition

1981 ◽  
Vol 19 (1) ◽  
pp. 1
Author(s):  
Won Young Choi ◽  
Young Kwan Jin ◽  
Ok Ran Lee ◽  
Woon Gyu Kim
Keyword(s):  
Comparative Analysis ◽  
Ascaris Suum ◽  
Protein Composition

scholarly journals Human U4/U6.U5 and U4atac/U6atac.U5 Tri-snRNPs Exhibit Similar Protein Compositions

2002 ◽  
Vol 22 (10) ◽  
pp. 3219-3229 ◽  
Author(s):  
Claudia Schneider ◽  
Cindy L. Will ◽  
Olga V. Makarova ◽  
Evgeny M. Makarov ◽  
Reinhard Lührmann
Keyword(s):  
Comparative Analysis ◽  
Common Ancestor ◽  
Sequence Similarity ◽  
Evolutionary Model ◽  
Protein Composition ◽  
Significant Sequence Similarity ◽  
Similar Protein ◽  
Specific Proteins

ABSTRACT In the U12-dependent spliceosome, the U4atac/U6atac snRNP represents the functional analogue of the major U4/U6 snRNP. Little information is available presently regarding the protein composition of the former snRNP and its association with other snRNPs. In this report we show that human U4atac/U6atac di-snRNPs associate with U5 snRNPs to form a 25S U4atac/U6atac.U5 trimeric particle. Comparative analysis of minor and major tri-snRNPs by using immunoprecipitation experiments revealed that their protein compositions are very similar, if not identical. Not only U5-specific proteins but, surprisingly, all tested U4/U6- and major tri-snRNP-specific proteins were detected in the minor tri-snRNP complex. Significantly, the major tri-snRNP-specific proteins 65K and 110K, which are required for integration of the major tri-snRNP into the U2-dependent spliceosome, were among those proteins detected in the minor tri-snRNP, raising an interesting question as to how the specificity of addition of tri-snRNP to the corresponding spliceosome is maintained. Moreover, immunodepletion studies demonstrated that the U4/U6-specific 61K protein, which is involved in the formation of major tri-snRNPs, is essential for the association of the U4atac/U6atac di-snRNP with U5 to form the U4atac/U6atac.U5 tri-snRNP. Subsequent immunoprecipitation studies demonstrated that those proteins detected in the minor tri-snRNP complex are also incorporated into U12-dependent spliceosomes. This remarkable conservation of polypeptides between minor and major spliceosomes, coupled with the absence of significant sequence similarity between the functionally analogous snRNAs, supports an evolutionary model in which most major and minor spliceosomal proteins, but not snRNAs, are derived from a common ancestor.

Comparative analysis of protein composition and digestibility of Ceratonia siliqua L. and Prosopis spp. seed germ flour

2019 ◽  
Vol 120 ◽  
pp. 188-195 ◽  
Author(s):  
Gianfranco Mamone ◽  
Leonardo Sciammaro ◽  
Salvatore De Caro ◽  
Luigia Di Stasio ◽  
Francesco Siano ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Protein Composition ◽  
Ceratonia Siliqua ◽  
Ceratonia Siliqua L

scholarly journals Comparative analysis of microRNA profiles between adult Ascaris lumbricoides and Ascaris suum

2014 ◽  
Vol 10 (1) ◽  
pp. 99 ◽  
Author(s):  
Chang-Chun Shao ◽  
Min-Jun Xu ◽  
Samer Alasaad ◽  
Hui-Qun Song ◽  
Lifei Peng ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Ascaris Lumbricoides ◽  
Ascaris Suum

scholarly journals COMPARATIVE ANALYSIS OF THE EXOSOMAL CARGO OF THE ESTROGEN-RESISTANT BREAST CANCER CELLS

2018 ◽  
Vol 17 (4) ◽  
pp. 36-40 ◽  
Author(s):  
S. E. Semina ◽  
N. A. Barlev ◽  
A. G. Mittenberg ◽  
M. A. Krasilnikov
Keyword(s):  
Breast Cancer ◽  
Comparative Analysis ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Protein Composition ◽  
Tamoxifen Treatment ◽  
Transfer Resistance ◽  
Exosomal Mirnas ◽  
Hormonal Resistance ◽  
Mcf 7

The exosomes involvement in the pathogenesis of tumors is based on their property to incorporate into the recipient cells resulting in the both genomic and epigenomic changes.  Earlier we have shown that exosomes from different types of estrogen-independent breast  cancer cells (MCF-7/T developed by long-term tamoxifen treatment, and MCF-7/M)  developed by metformin treatment were able to transfer resistance to the parent MCF-7  cells. To elucidate the common features of the both types of resistant exosomes, the  proteome and microRNA cargo of the control and both types of the resistant exosomes were  analyzed. Totally, more than 400 proteins were identified in the exosome samples. Of these  proteins, only two proteins, DMBT1 (Deleted in Malignant Brain Tumors 1) and THBS1  (Thrombospondin-1), were commonly expressed in the both resistant exosomes (less than  5% from total DEPs) demonstrating the unique protein composition of each type of the resistant exosomes. The comparative analysis of the miRNA differentially expressed in  the both MCF-7/T and MCF-7/M resistant exosomes revealed 180 up-regulated and 202  down-regulated miRNAs. Among them, 4 up-regulated and 8 down-regulated miRNAs were  associated with progression of hormonal resistance of breast tumors. The bioinformatical  analysis of 4 up-regulated exosomal miRNAs revealed 2 miRNAs, mir- 101and mir-181b, which up-regulated PI3K signaling  supporting the key role of PI3K/Akt in the development of the resistant phenotype of breast cancer cells.

scholarly journals Different Research Approaches in Unraveling the Venom Proteome of Naja ashei

Biomolecules ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1282
Author(s):  
Konrad Kamil Hus ◽  
Łukasz Marczak ◽  
Vladimír Petrilla ◽  
Monika Petrillová ◽  
Jaroslav Legáth ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Protein Composition ◽  
Process Data ◽  
Qualitative And Quantitative ◽  
Dynamic Development ◽  
Proteomic Data ◽  
Ms Analysis ◽  
New Information ◽  
Proteomic Methods

The dynamic development of venomics in recent years has resulted in a significant increase in publicly available proteomic data. The information contained therein is often used for comparisons between different datasets and to draw biological conclusions therefrom. In this article, we aimed to show the possible differences that can arise, in the final results of the proteomic experiment, while using different research workflows. We applied two software solutions (PeptideShaker and MaxQuant) to process data from shotgun LC-MS/MS analysis of Naja ashei venom and collate it with the previous report concerning this species. We were able to provide new information regarding the protein composition of this venom but also present the qualitative and quantitative limitations of currently used proteomic methods. Moreover, we reported a rapid and straightforward technique for the separation of the fraction of proteins from the three-finger toxin family. Our results underline the necessary caution in the interpretation of data based on a comparative analysis of data derived from different studies.

scholarly journals Prevalence of Swine Nematodes in Moscow, Russia

2021 ◽  
Vol 14 (4) ◽  
pp. 1463-1467
Author(s):  
Igor G. Glamazdin
Keyword(s):  
Comparative Analysis ◽  
Detailed Analysis ◽  
Ascaris Suum ◽  
Moscow Region ◽  
Helminth Eggs ◽  
Current Prevalence ◽  
Prophylactic Measures

To continue the fight against pig helminthiasis, a detailed analysis of the current prevalence of nematodes in these productive animals is required. The assessment of the material after the technological slaughter of pigs kept in the Moscow region revealed the presence of parasitization in their intestines of four nematodes (Ascaris suum, Metastrongylus spp., Trichocephalus spp., Strongyloides spp.) In the form of monoinvasion and any combination thereof. The total infestation of pigs with intestinal nematodes was 55.24%. Trichocytic invasion (Trichocephalus spp.) Occurred in pigs most often (23.77%), ascarous invasion (Ascaris suum) reached 22.38%, strongyloid invasion (Strongyloides spp.) Was noted in 9.09% of cases, metastrongylous (Metastrongylus spp.) was present in 1.75% of cases. In conditions of an increase in the severity of monoinvasion, the size of helminth eggs decreased in Trichuris: size in length by 5.9%, size in width by 7.4%, in Ascaris: length by 4.9%, width by 4.6%, in strongulata eggs a decrease in size is also noted. In the case of mixed Ascaris-Metastrongylous invasion, there was a clear antagonistic relationship, manifested in a decrease in the size of Ascaris eggs. The size of the eggs of Trichuris in the case of an invasion mixed with Ascaris decreased, which is apparently associated with the development of antagonism between them. Comparative analysis of information on the prevalence of nematodes in pigs in farms of the Moscow region and their size can help to increase the effectiveness of treatment and prophylactic measures against helminthiasis, and can be taken into account in the course of further examination of pigs in the Moscow.

A comparative analysis of various developmental stages of Schistosoma mansoni with respect to their protein composition

Parasitology ◽  
1977 ◽  
Vol 75 (3) ◽  
pp. 339-343 ◽  
Author(s):  
Andreas Ruppel ◽  
Donato Cioli
Keyword(s):  
Comparative Analysis ◽  
Schistosoma Mansoni ◽  
Sodium Dodecyl Sulphate ◽  
Developmental Stages ◽  
Sodium Dodecyl ◽  
Protein Composition ◽  
Protein Patterns ◽  
High Degree ◽  
Slab Gels

SummaryThe protein composition of Schistosoma mansoni was analysed by electrophoresis in polyacrylamide slab gels in the presence of sodium dodecyl sulphate. A high degree of identity existed between protein patterns obtained from immature and adult parasites of the two sexes. Some stage-and sex-specific components were identified and their possible origin discussed.

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