Interactions between the plasma proteins of Biomphalaria glabrata (Gastropoda) and the sporocyst tegument of Schistosoma mansoni (Trematoda)

Parasitology ◽  
1986 ◽  
Vol 92 (3) ◽  
pp. 653-664 ◽  
Author(s):  
C. J. Bayne ◽  
E. S. Loker ◽  
Mary A. Yui
Keyword(s):  
Schistosoma Mansoni ◽  
Plasma Proteins ◽  
Biomphalaria Glabrata ◽  
Rabbit Antibody ◽  
Tegumental Surface ◽  
Immunological Interactions ◽  
Resistant Strains ◽  
Host Parasite ◽  
Susceptibility And Resistance

SUMMARYThe tegumental surface of Schistosoma mansoni sporocysts is the site of both nutritive and immunological interactions with haemolymph cells and plasma of Biomphalaria glabrata, the schistosome intermediate host. Within minutes of being placed in host plasma, sporocysts acquire plasma antigens, and within 3 h host plasma antigens are present on the surface at near steady state. Though a wide variety of peptides is acquired, there is selection. Furthermore, some differences occur in the peptides acquired from the plasma of susceptible and resistant strains of snail. Acquired antigens are rapidly processed, and are predominantly undetectable in tegumental extracts after a few hours. In contrast, rabbit antibody on sporocysts remains in situ for at least 48 h, so under some conditions there is stable expression of certain tegumental antigenic determinants.These data, obtained using antibodies to snail plasma antigens and to sporocyst tegumental antigens, are discussed in the light of current ideas on the cellular and molecular basis of susceptibility and resistance in this host#parasite system.

scholarly journals Spatial expression pattern of serine proteases in the blood fluke Schistosoma mansoni determined by fluorescence RNA in situ hybridization

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Lenka Ulrychová ◽  
Pavel Ostašov ◽  
Marta Chanová ◽  
Michael Mareš ◽  
Martin Horn ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Schistosoma Mansoni ◽  
Rna Sequencing ◽  
Serine Proteases ◽  
Expression Patterns ◽  
High Sensitivity ◽  
Host Parasite Interactions ◽  
Highly Sensitive ◽  
Host Parasite

Abstract Background The blood flukes of genus Schistosoma are the causative agent of schistosomiasis, a parasitic disease that infects more than 200 million people worldwide. Proteases of schistosomes are involved in critical steps of host–parasite interactions and are promising therapeutic targets. We recently identified and characterized a group of S1 family Schistosoma mansoni serine proteases, including SmSP1 to SmSP5. Expression levels of some SmSPs in S. mansoni are low, and by standard genome sequencing technologies they are marginally detectable at the method threshold levels. Here, we report their spatial gene expression patterns in adult S. mansoni by the high-sensitivity localization assay. Methodology Highly sensitive fluorescence in situ RNA hybridization (FISH) was modified and used for the localization of mRNAs encoding individual SmSP proteases (including low-expressed SmSPs) in tissues of adult worms. High sensitivity was obtained due to specifically prepared tissue and probes in combination with the employment of a signal amplification approach. The assay method was validated by detecting the expression patterns of a set of relevant reference genes including SmCB1, SmPOP, SmTSP-2, and Sm29 with localization formerly determined by other techniques. Results FISH analysis revealed interesting expression patterns of SmSPs distributed in multiple tissues of S. mansoni adults. The expression patterns of individual SmSPs were distinct but in part overlapping and were consistent with existing transcriptome sequencing data. The exception were genes with significantly low expression, which were also localized in tissues where they had not previously been detected by RNA sequencing methods. In general, SmSPs were found in various tissues including reproductive organs, parenchymal cells, esophagus, and the tegumental surface. Conclusions The FISH-based assay provided spatial information about the expression of five SmSPs in adult S. mansoni females and males. This highly sensitive method allowed visualization of low-abundantly expressed genes that are below the detection limits of standard in situ hybridization or by RNA sequencing. Thus, this technical approach turned out to be suitable for sensitive localization studies and may also be applicable for other trematodes. The results suggest that SmSPs may play roles in diverse processes of the parasite. Certain SmSPs expressed at the surface may be involved in host–parasite interactions. Graphic abstract

scholarly journals QUANTIFICATION OF THE POPULATION AND PHAGOCYTARY ACTIVITY OF HEMOCYTES OF RESISTANT AND SUSCEPTIBLE STRAINS OF Biomphalaria glabrata AND Biomphalaria tenagophila INFECTED WITH Schistosoma mansoni

1997 ◽  
Vol 39 (4) ◽  
pp. 197-202 ◽  
Author(s):  
F. S. de M. BEZERRA ◽  
J. A. NOGUEIRA-MACHADO ◽  
M. M. CHAVES ◽  
R. L. MARTINS ◽  
P. M. Z. COELHO
Keyword(s):  
Schistosoma Mansoni ◽  
Cell Population ◽  
Metabolic Activity ◽  
Post Infection ◽  
Biomphalaria Glabrata ◽  
Determinant Factors ◽  
Circulating Cells ◽  
Resistant Strains ◽  
First Time

Among the determinant factors in the resistance and susceptibility of Biomphalaria to Schistosoma mansoni, hemocytes play an important role. Aiming at studying S. mansoni/Biomphalaria interactions related to hemocytes, the first step is certainly connected with the standardization of this cell population in uninfected Biomphalaria. In this way, quantification of this cell population in hemolymph, as well as its phagocitary capacity, have been determined for the first time. Furthermore, using susceptible and resistant strains of B. glabrata and B. tenagophila, the hemocytegram and phagocytary capacity of hemocytes after infection with S. mansoni were determined too. Resistant and susceptible strains of B.glabrata (BA and BH, respectively), as well as resistant and susceptible strains of B. tenagophila (Taim and CF, respectively) were infected with 10 miracidia of the LE and SJ strains of S. mansoni, respectively. These infected snails and respective uninfected controls were assessed in relation to the number of circulating hemocytes and alteration in the phagocytary capacity, by using Zymozan and MTT. Reading was taken by means of a spectrophotometer at 5 hours and 1,2,5,10,20 and 30 days after infection. The results showed a decrease in population of the circulating phagocytary cells, 5 hours after infection. One day post-infection, the circulating cells of the susceptible snails showed an increased metabolic activity, but the same event could not be observed in the resistant strains. In the subsequent observation periods, significant differences among the strains studied could not be observed until the end of the experiment

scholarly journals Strain variation in the infectivity of Schistosoma mansoni for Biomphalaria glabrata

1988 ◽  
Vol 30 (2) ◽  
pp. 86-90 ◽  
Author(s):  
Luiz Candido de Souza Dias ◽  
John I. Bruce ◽  
Gerald C. Coles
Keyword(s):  
Schistosoma Mansoni ◽  
Puerto Rican ◽  
Biomphalaria Glabrata ◽  
The Other ◽  
Drug Resistant ◽  
Strain Variation ◽  
Resistant Strains ◽  
Drug Resistant Strains

Five strains of Schistosoma mansoni resistant and susceptible to schistosomicides were studied for infectivity of 2 strains of Biomphalaria glabrata one of Puerto Rican origin and the other of Brazilian origin. Puerto Rican strains of S. Mansoni developed more slowly and had a lower infectivity in Brazilian B. glabrata than did the Brazilian S. mansoni. However, Brazilian S. Mansoni developed as well in Puerto Rican snails as in Brazilian snails, indicating that drug resistant strains could easily be moved by travel of infected persons from one area to another.

Transient non-susceptibility toSchistosoma mansoniassociated with atrial amoebocytic accumulations in the snail hostBiomphalaria glabrata

Parasitology ◽  
1987 ◽  
Vol 95 (3) ◽  
pp. 499-505 ◽  
Author(s):  
C. S. Richards ◽  
D. J. Minchella
Keyword(s):  
Population Dynamics ◽  
Schistosoma Mansoni ◽  
Genetic Control ◽  
Old Age ◽  
Biomphalaria Glabrata ◽  
Egg Laying ◽  
Snail Population ◽  
Host Parasite ◽  
Variable Susceptibility

SUMMARYIn someBiomphalaria glabrata–Schistosoma mansonicombinations snails are susceptible to infection as juveniles, but have variable susceptibility as adults. These snails become non-susceptible at the onset of egg-laying and typically revert to susceptibility in old age. Certain stocks ofB. glabratahave the capacity to form amoebocytic accumulations in the atrium, and this ability is under genetic control. The atrial amoebocytic accumulations are transitory, typically appearing at onset of egg-laying and disappearing after a few months. A snail stock which has genetic tendencies for both adult variable susceptibility and atrial amoebocytic accumulations was studied. An association between the time of occurrence of adult non-susceptibility and atrial accumulation is revealed as snails never became infected withS. mansoniwhen amoebocytic accumulations were present. Developing parasites, however, were not necessarily encapsulated and destroyed by amoebocytes. Some sporocysts were able to delay development until the amoebocytic accumulations disappeared. The timing of atrial amoebocytic accumulations and resulting transient non-susceptibility in this host-parasite combination could influence snail population dynamics.

SURFACE MEMBRANE PROTEINS OF BIOMPHALARIA GLABRATA EMBRYONIC CELLS BIND FUCOSYL DETERMINANTS ON THE TEGUMENTAL SURFACE OF SCHISTOSOMA MANSONI PRIMARY SPOROCYSTS

2007 ◽  
Vol 93 (4) ◽  
pp. 832-840 ◽  
Author(s):  
Maria G. Castillo ◽  
Xiao-Jun Wu ◽  
Nathalie Dinguirard ◽  
A. Kwame Nyame ◽  
Richard D. Cummings ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Schistosoma Mansoni ◽  
Surface Membrane ◽  
Biomphalaria Glabrata ◽  
Embryonic Cells ◽  
Tegumental Surface

Geographical variations in infectivity and susceptibility in the host-parasite system Schistosoma mansoni/Biomphalaria glabrata: no evidence for local adaptation

Parasitology ◽  
2006 ◽  
Vol 133 (3) ◽  
pp. 313-319 ◽  
Author(s):  
F. PRUGNOLLE ◽  
T. DE MEEÛS ◽  
J.P. POINTIER ◽  
P. DURAND ◽  
A. ROGNON ◽  
...  
Keyword(s):  
Schistosoma Mansoni ◽  
Local Adaptation ◽  
Genetic Factors ◽  
Biomphalaria Glabrata ◽  
Metapopulation Dynamics ◽  
Geographical Variations ◽  
Transplantation Experiment ◽  
Host Parasite ◽  
Parasite Populations ◽  
Transplantation Experiments

We investigated local adaptation in the spatially structured natural Biomphalaria glabrata/Schistosoma mansoni host-parasite system in the marshy forest focus of Guadeloupe using cross-transplantation experiments. We demonstrated strong and highly significant variations in susceptibility/infectivity of host and parasite populations, respectively, but found no evidence of local adaptation neither for S. mansoni nor for B. glabrata. Environmental as well as genetic factors are discussed to explain susceptibility/infectivity variations between both host and parasite populations. The absence of local adaptation is discussed in relation to the metapopulation dynamics of both host and parasite, in particular their relative rates of dispersal at the scale under scrutiny. Our study constitutes the first cross-transplantation experiment concerning this host-parasite system of which both hosts and parasites came directly from the wild, excluding laboratory generations and experimental host passages.

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