Studies on Eimeria praecox Johnson, 1930, in the chicken

Parasitology ◽  
1967 ◽  
Vol 57 (2) ◽  
pp. 351-361 ◽  
Author(s):  
P. L. Long
Keyword(s):  
Small Intestine ◽  
Life Cycle ◽  
Small Dose ◽  
Technical Assistance ◽  
Host Immunity ◽  
Single Exposure ◽  
Three Generations ◽  
Large Numbers ◽  
High Degree ◽  
First Time

E. praeox has been isolated in Britain for the first time and is similar in regard to its oocyst size, prepatent time, life-cycle and the development of host immunity to the reports of Tyzzer et al. (1932). Oocysts had mean dimensions of 20·4 × 17·45 μm and the first oocysts were discharged 83½ h after infection.Oocyst production and life-cycle studies suggest that at least three generations of schizogony precede gametogony and that at least one further generation of schizogony is needed to explain the oocyst reproduction resulting from a small dose of oocysts. Young chickens 1½–3 weeks of age were not such suitable hosts as older chickens (6 weeks) and the reproduction of the parasite was seriously reduced when large numbers (e.g. 105, 106) were used to infect chickens. A high degree of resistance to reinfection occurred after a single exposure to infection.Sporozoite infections of the caeca or cloaca resulted in the infection of the usual small intestine site; there was no development at the site of inoculation and attempts to infect the embryo allantois did not succeed.I wish to thank Dr M. Elaine Rose for the surgery required for the caecal infections, for her interest throughout and for help with the manuscript; Dr R. F. Gordon for help with the manuscript and permission to publish and Mr B. J. Millard for skilled technical assistance.

Variability of Behaviour of Aggregating Cellular Slime Moulds

1957 ◽  
Vol s3-98 (44) ◽  
pp. 393-406
Author(s):  
B. M. SHAFFER
Keyword(s):  
Life Cycle ◽  
Environmental Conditions ◽  
The Third ◽  
Slime Moulds ◽  
Large Numbers ◽  
Wide Range ◽  
Cellular Slime ◽  
High Degree ◽  
Cellular Slime Moulds ◽  
Geometrical Relationships

1. A description is given of the development of certain structures--centre, stream, and ring--formed by the amoebae of four species of Dictyostelium and Polysphondyliumduring aggregation, and of the outcome of experimentally cutting a stream, recombining lengths of stream in simple geometrical relationships, and excising a centre. 2. In all cases there is a high degree of intraspecific variation in behaviour, and it is not possible to predict, except in terms of rough probability, what the future course of development will be. 3. An amoeba at this stage of the life cycle can exist in one of four main states. In the first, it is unaggregated and cannot react chemotactically; in the second, it is unaggregated but can react; in the third, it is in a stream moving towards a centre or has properties similar to those of a stream cell; and in the fourth, it is in a centre and nearly stationary. Though an enormous variety of patterns may be produced by large numbers of interacting amoebae, their behavioural repertory may be considered, without too much simplification, to be restricted to approaching these four states in various sequences and at various rates. 4. Most of these sequences can be observed over a wide range of environmental conditions. Changes in humidity produce the greatest effect: the drier the culture, the stronger the drift from the first state to the fourth. 5. It is suggested that variability is occasioned by the simplicity of the mechanisms controlling development.

The life-cycle of Bucephaloides gracilescens (Rudolphi, 1819) Hopkins, 1954 (Digenea: Gasterostomata)

Parasitology ◽  
1974 ◽  
Vol 68 (1) ◽  
pp. 1-12 ◽  
Author(s):  
R. A. Matthews
Keyword(s):  
Life Cycle ◽  
Intermediate Host ◽  
Host Specificity ◽  
Comparative Morphology ◽  
Rock Pools ◽  
Second Intermediate Host ◽  
High Degree ◽  
First Time ◽  
First Intermediate Host

The cercaria and sporocyst of Bucephaloides gracilescens are described from Abra alba (Wood). Observations were made on the behaviour of the cercaria and the mechanism of release from the first intermediate host. The metacercaria was obtained experimentally for the first time using Ciliata mustela (L.), a species of Gadidae from rock pools, as second intermediate host. It has not previously been recorded from this fish. Unsuccessful attempts were made to infect species of fishes from three other families, namely, Bothidae, Pleuronectidae and Gobiidae, confirming the high degree of host specificity of the metacercaria to Gadidae. The metacercaria, its development and effect on the host are briefly discussed. It was linked with the adult on the basis of comparative morphology and ecology of the hosts.

Genetic Diversity of Basil (Ocimum spp. - Lamiaceae) Based on RAPD Markers and Volatile Oil Constituents

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 492f-493
Author(s):  
Roberto F. Vieira ◽  
James E. Simon ◽  
Peter Goldsbrough ◽  
Antonio Figueira
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Rapd Markers ◽  
Volatile Oil ◽  
Essential Oil Composition ◽  
Oil Composition ◽  
Traditional Medicines ◽  
The Tropics ◽  
High Degree ◽  
First Time

Essential oils extracted from basil (Ocimum spp.) by steam distillation are used to flavor foods, oral products, in fragrances, and in traditional medicines. The genus Ocimum contains around 30 species native to the tropics and subtropics, with some species naturalized and/or cultivated in temperate areas. Interand intraspecific hybridization have created significant confusion in the botanical systematics of this genus. Taxonomy of basil (O. basilicum) is also complicated by the existence of numerous varieties, cultivars, and chemotypes within the species that do not differ significantly in morphology. In this study we are using RAPD markers and volatile oil composition to characterize the genetic diversity among the most economically important Ocimum species. We hypothesize that the genetic similarity revealed by molecular markers will more accurately reflect the morphological and chemical differences in Ocimum than essential oil composition per se. Preliminary research using five Ocimum species, four undetermined species, and eight varieties of O. basilicum (a total of 19 accessions) generated 107 polymorphic fragments amplified with 19 primers. RAPDs are able to discriminate between Ocimum species, but show a high degree of similarity between O. basilicum varieties. The genetic distance between nine species and among 55 accessions within the species O. americanum, O. basilicum, O. campechianum, O. × citriodorum, O. gratissimum, O. kilimandscharium, O. minimum, O. selloi, and O. tenuiflorum will be analyzed by matrix of similarity and compared to the volatile oil profile. This research will for the first time apply molecular markers to characterize the genetic diversity of Ocimum associate with volatile oil constituent.

Development and evaluation of an immunoenzymometric assay for the chicken progesterone receptor

1991 ◽  
Vol 129 (2) ◽  
pp. 189-196 ◽  
Author(s):  
M. K. Bläuer ◽  
P. J. Tuohimaa ◽  
P. J. Vilja
Keyword(s):  
Monoclonal Antibodies ◽  
Small Intestine ◽  
Horseradish Peroxidase ◽  
Progesterone Receptor ◽  
Ligand Binding ◽  
Binding Assay ◽  
Ligand Binding Assay ◽  
Coefficients Of Variation ◽  
First Time ◽  
Determination Range

ABSTRACT A specific and sensitive immunoenzymometric assay (IEMA) was developed for measuring the quantity of chicken progesterone receptor (PR) in tissue cytosol. The assay uses two monoclonal antibodies to the PR. One is used to capture the PR. The second (labelled with biotin) reacts first with the captured receptor and subsequently with avidin-labelled horseradish peroxidase to provide an enzymatic end-point. The method has a determination range from 0·3 to 60 pmol/l. Intra- and interassay coefficients of variation were 3·7% and 9·0% respectively. The assay can be performed with equal results as a rapid (3 h) or an overnight procedure. The IEMA is convenient, especially for signal measurement and the calculation of results. No ultracentrifugation of samples is needed, since the IEMA can be performed on low-speed cytosol samples. Assay results correlated well (r = 0·927) with those obtained by the conventional ligand-binding assay used in our laboratory. Similar results were obtained with the IEMA and the ligand-binding assay after exposure of cytosol samples to increased temperatures: at 20 °C the PR remained stable for the 4-h period examined, whereas at 37 °C almost complete degradation of the PR was observed in 30 min. Being more than 100 times as sensitive as the ligand-binding assay, the IEMA enabled the quantification of PR for the first time in such tissues as the bursa and small intestine even of immature animals. Journal of Endocrinology (1991) 129, 189–196

scholarly journals The First Record of Echinococcus ortleppi (G5) Tapeworms in Grey Wolf (Canis lupus)

Pathogens ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 853
Author(s):  
Jacek Karamon ◽  
Małgorzata Samorek-Pieróg ◽  
Jacek Sroka ◽  
Ewa Bilska-Zając ◽  
Joanna Dąbrowska ◽  
...  
Keyword(s):  
Small Intestine ◽  
Molecular Identification ◽  
Canis Lupus ◽  
Sequence Comparison ◽  
First Record ◽  
Grey Wolf ◽  
South Eastern ◽  
Counting Technique ◽  
Small Intestines ◽  
First Time

The aim of this study is to confirm the presence and molecular identification of Echinococcus tapeworms in wolves from south-eastern Poland. An investigation was carried out on the intestines of 13 wolves from south-eastern Poland. The small intestines were divided into three equal segments. Each segment was separately examined using the sedimentation and counting technique (SCT). The detected Echinococcus tapeworms were isolated and identified by PCRs and sequencing (nad1 and cox1 genes). Additionally, DNA isolated from the feces of wolves positive for Echinococcus tapeworms was examined with two diagnostic PCRs. The intestines of one wolf were positive for E. granulosus s.l. when assessed by SCT; the intestine was from a six-year-old male wolf killed in a communication accident. We detected 61 adult tapeworms: 42 in the anterior, 14 in the middle, and 5 in the posterior parts of the small intestine. The PCRs conducted for cox1 and nad1 produced specific products. A sequence comparison with the GenBank database showed similarity to the deposited E. ortleppi (G5) sequences. An analysis of the available phylogenetic sequences showed very little variation within the species of E. ortleppi (G5), and identity ranged from 99.10% to 100.00% in the case of cox1 and from 99.04 to 100.00% in the case of nad1. One of the two diagnostic PCRs used and performed on the feces of Echinococcus-positive animals showed product specific for E. granulosus. This study showed the presence of adult E. ortleppi tapeworms in wolves for the first time.

Research of Graphic User Interface of Carbon Dioxide Emission Evaluation Model in Construction Life Cycle Based on Matlab

2013 ◽  
Vol 689 ◽  
pp. 546-550
Author(s):  
Xue Hong Gan ◽  
Wei Wang ◽  
Shi Mei Liu
Keyword(s):  
Carbon Dioxide ◽  
Life Cycle ◽  
Evaluation Model ◽  
Carbon Dioxide Emission ◽  
Graphic User Interface ◽  
Property Management ◽  
Low Carbon ◽  
Management Responsibility ◽  
Residential Quarter ◽  
First Time

To build carbon dioxide emission evaluation model in construction life cycle is the key link to make low-carbon research of theoretical studies head towards practical application. For the first time, residential quarter is selected as the object in the study, carbon dioxide emission management responsibility of owner and property being considered and carbon dioxide emission evaluation model in construction life cycle based on matlab as well as database of carbon dioxide factor being built initially. Net carbon dioxide emission information will be shown timely, then carbon dioxide emission and absorption can be adjusted by property management accordingly.

The habits and habitats of the opisthobranch molluscs of the British Solomon Islands

1969 ◽  
Vol 255 (800) ◽  
pp. 541-548 ◽  
Keyword(s):  
Natural History ◽  
Solomon Islands ◽  
Gut Contents ◽  
Numerical Evidence ◽  
Large Numbers ◽  
Different Types ◽  
Opisthobranch Molluscs ◽  
First Time ◽  
Reef Formation

Often bizarre in shape and gaudily coloured, the opisthobranchs of the tropical IndoPacific have always been attractive subjects for the specialist worker and for writers of popular natural history. There is certainly no lack of papers describing their anatomy and systematics (Marcus & Burch (1965) give a fairly full bibliography), but only in those by Risbec (1928 a, b , 1951, 1953) are there descriptions of where or how they live and his statements are brief and rather vague. I collected opisthobranchs so as to record them for the British Solomon Islands for the first time and, whenever possible, I noted their habits and habitats. My observations on gut contents are rather scanty as I did not wish to damage any of the few specimens of each species collected. From an examination of ten different types of reef formation I discovered that opisthobranchs are common in only two places; (1) the crest of a semi-exposed reef where algae occur in pools, and (2) the flat of a sheltered or semi-sheltered reef. At each of these sites several distinct habitats can be distinguished. Unfortunately my recognition of these habitats is not supported by strong numerical evidence as none of the 55 species occurred in large numbers.

Characterization of SrBi2Nb2O9 Thin Films Prepared by Sol Gel Technique

1996 ◽  
Vol 459 ◽  
Author(s):  
E. Ching-Prado ◽  
W. Pérez ◽  
A. Reynés-Figueroa ◽  
R. S. Katiyar ◽  
D. Ravichandran ◽  
...  
Keyword(s):  
Thin Films ◽  
Raman Band ◽  
Sol Gel ◽  
Orthorhombic Symmetry ◽  
X Ray Diffraction ◽  
Ft Ir ◽  
Gel Technique ◽  
High Degree ◽  
First Time

ABSTRACTThin films of SrBi2Nb2O9 (SBN) with thicknesses of 0.1, 0.2, and 0.4 μ were grown by Sol-gel technique on silicon, and annealed at 650°C. The SBN films were investigated by Raman scatering for the first time. Raman spectra in some of the samples present bands around 60, 167, 196, 222, 302, 451, 560, 771, 837, and 863 cm−1, which correspond to the SBN formation. The study indicates that the films are inhomogeneous, and only in samples with thicknesses 0.4 μ the SBN material was found in some places. The prominent Raman band around 870 cm−1, which is the A1g mode of the orthorhombic symmetry, is assigned to the symmetric stretching of the NbO6 octahedrals. The frequency of this band is found to shift in different places in the same sample, as well as from sample to sample. The frequency shifts and the width of the Raman bands are discussed in term of ions in non-equilibrium positions. FT-IR spectra reveal a sharp peak at 1260 cm−1, and two broad bands around 995 and 772 cm−1. The bandwidths of the latter two bands are believed to be associated with the presence of a high degree of defects in the films. The experimental results of the SBN films are compared with those obtained in SBT (T=Ta) films. X-ray diffraction and SEM techniques are also used for the structural characterization.

The Synthesis and Some Properties of the First Boron-Stabilized Alkenyl Carbanions

1992 ◽  
Vol 45 (1) ◽  
pp. 57 ◽  
Author(s):  
A Pelter ◽  
K Smith ◽  
DE Parry ◽  
KD Jones
Keyword(s):  
Type Structure ◽  
High Degree ◽  
First Time

Calculations indicate a high degree of stabilization of H2BC=CH2 and that it has an allene -type structure H2B=C=CH2. Routes to boron-stabilized alkenyl carbanions have been established for the first time, and alkylation and protonation studies are described.

scholarly journals CYTOPLASMIC DNA SYNTHESIS IN AMOEBA PROTEUS

1962 ◽  
Vol 15 (3) ◽  
pp. 525-534 ◽  
Author(s):  
M. Rabinovitch ◽  
W. Plaut
Keyword(s):  
Dna Synthesis ◽  
Tritiated Thymidine ◽  
Amoeba Proteus ◽  
Living Cells ◽  
Large Numbers ◽  
Cytoplasmic Dna ◽  
Basic Dyes ◽  
Nuclease Digestion ◽  
High Degree ◽  
Very High

The incorporation of tritiated thymidine in Amoeba proteus was reinvestigated in order to see if it could be associated with microscopically detectable structures. Staining experiments with basic dyes, including the fluorochrome acridine orange, revealed the presence of large numbers of 0.3 to 0.5 µ particles in the cytoplasm of all cells studied. The effect of nuclease digestion on the dye affinity of the particles suggests that they contain DNA as well as RNA. Centrifugation of living cells at 10,000 g leads to the sedimentation of the particles in the centrifugal third of the ameba near the nucleus. Analysis of centrifuged cells which had been incubated with H3-thymidine showed a very high degree of correlation between the location of the nucleic acid-containing granules and that of acid-insoluble, deoxyribonuclease-sensitive labeled molecules and leads to the conclusion that cytoplasmic DNA synthesis in Amoeba proteus occurs in association with these particles.

Sign in / Sign up

Export Citation Format

Share Document