Life cycle ofCryptosporidium murisin two rodents with different responses to parasitization

Parasitology ◽  
2013 ◽  
Vol 141 (2) ◽  
pp. 287-303 ◽  
Author(s):  
JANKA MELICHEROVÁ ◽  
JANA ILGOVÁ ◽  
MARTIN KVÁČ ◽  
BOHUMIL SAK ◽  
BŘETISLAV KOUDELA ◽  
...  

SUMMARYThis study focuses on mapping the life cycle ofCryptosporidium murisin two laboratory rodents; BALB/c mice and the southern multimammate ratMastomys coucha, differing in their prepatent and patent periods. Both rodents were simultaneously experimentally inoculated with viable oocysts ofC. muris(strain TS03). Animals were dissected and screened for the presence of the parasite using a combined morphological approach and nested PCR (SSU rRNA) at different times after inoculation. The occurrence of first developmental stages ofC. murisin stomach was detected at 2·5 days post-infection (dpi). The presence of Type II merogony, appearing 36 h later than Type I merogony, was confirmed in both rodents. Oocysts exhibiting different size and thickness of their wall were observed from 5 dpi onwards in stomachs of both host models. The early phase of parasitization in BALB/c mice progressed rapidly, with a prepatent period of 7·5–10 days; whereas inM. coucha, the developmental stages ofC. muriswere first observed 12 h later in comparison with BALB/c mice and prepatent period was longer (18–21 days). Similarly, the patent periods of BALB/c mice andM. couchadiffered considerably, i.e. 10–15 daysvschronic infection throughout the life of the host, respectively.

Genetics ◽  
1974 ◽  
Vol 77 (4) ◽  
pp. 639-650
Author(s):  
Satoshi Harashima ◽  
Yasuhisa Nogi ◽  
Yasuji Oshima

ABSTRACT There are four types of life cycles in Saccharomyces cerevisiae and its related species. A perfect homothallic life cycle (the Ho type) is observed in the classic D strain. Two other types show semi-homothallism; one of them shows a 2-homothallic diploid:2α heterothallic haploid segregation (the Hp type) and another, a 2-homothallic:2a segregation (the Hq type). In the segregants from these Ho, Hp, and Hq diploids, each homothallic segregant shows the same segregation pattern as its parental diploid. The fourth type has a heterothallic life cycle showing a 2a:2α segregation and the diploids are produced by the fusion of two haploid cells of opposite mating types. The diploids prepared by the crosses of α Hp (an α haploid segregant from the Hp diploid) to a Hq (an a haploid from the Hq diploid) segregated two types (Type I and II) of the Ho type homothallic clone among their meiotic segregants. Genetic analyses were performed to investigate this phenomenon and the genotypes of the Ho type homothallic clones of Type I and Type II. Results of these genetic analyses have been most adequately explained by postulating three kinds of homothallic genes, each consisting of a single pair of alleles, HO/ho, HMα/hmα, and HMa/hma, respectively. One of them, the HMα locus, was proved to be loosely linked (64 stranes) to the mating-type locus. A spore having the HO hmα hma genotype gives rise to an Ho type homothallic diploid (Type I), the same as in the case of the D strain which has the HO HMα HMa genotype (Type II). A spore having the a HO hmα HMa or α HO HMα hma genotype will produce an Hp or Hq type homothallic diploid culture, respectively. The other genotypes, a HO HMα hma, α HO hmα HMa, and the genotypes combined with the ho allele give a heterothallic character to the spore culture. A possible molecular hypothesis for the mating-type differentiation with the controlling elements produced by the HMα and HMa genes is proposed.


2015 ◽  
Vol 83 (5) ◽  
pp. 2148-2155 ◽  
Author(s):  
Barbara A. Fox ◽  
David J. Bzik

Live attenuated vaccine strains, such as type I nonreplicating uracil auxotroph mutants, are highly effective in eliciting lifelong immunity to virulent acute infection byToxoplasma gondii. However, it is currently unknown whether vaccine-elicited immunity can provide protection against acute infection and also prevent chronic infection. To address this problem, we developed nonreverting, nonreplicating, live attenuated uracil auxotroph vaccine strains in the type II Δku80genetic background by targeting the deletion of the orotidine 5′-monophosphate decarboxylase (OMPDC) and uridine phosphorylase (UP) genes. Deletion ofOMPDCinduced a severe uracil auxotrophy with loss of replication, loss of virulence in mice, and loss of the ability to develop cysts and chronic infection. Vaccination of mice using type II Δku80Δompdcmutants stimulated a fully protective CD8+T cell-dependent immunity that prevented acute infection by type I and type II strains ofT. gondii, and this vaccination also severely reduced or prevented cyst formation after type II challenge infection. Nonreverting, nonreplicating, and non-cyst-forming Δompdcmutants provide new tools to examine protective immune responses elicited by vaccination with a live attenuated type II vaccine.


mBio ◽  
2019 ◽  
Vol 10 (4) ◽  
Author(s):  
Barbara A. Fox ◽  
Rebekah B. Guevara ◽  
Leah M. Rommereim ◽  
Alejandra Falla ◽  
Valeria Bellini ◽  
...  

ABSTRACTToxoplasma gondiievades host immunity to establish a chronic infection. Here, we assessed the role of parasitophorous vacuole (PV) membrane (PVM)- and intravacuolar network (IVN) membrane-localized dense granule (GRA) proteins in the development of acute and chronicToxoplasmainfection. Deletion of PVM-associated GRA3, GRA7, GRA8, and GRA14 or IVN membrane-associated GRA2, GRA9, and GRA12 in the low-virulence type II Prugniaud (Pru) strain induced severe defects in the development of chronic-stage cystsin vivowithout affecting the parasite growth rate or the ability to differentiate into cystsin vitro. Acute virulence of the PruΔgra2, PruΔgra3, and PruΔgra4mutants was reduced but not abolished. In contrast, the PruΔgra12mutant was avirulent in mice and PruΔgra12parasites failed to establish a chronic infection. High-virulence type I strain RHΔgra12parasites also exhibited a major defect in acute virulence. In gamma interferon (IFN-γ)-activated macrophages, type I RHΔgra12and type II PruΔgra12parasites resisted the coating of the PVM with host immunity-related GTPases as effectively as the parental type I RHΔku80and type II PruΔku80strains, respectively. Despite this resistance, Δgra12PVs ultimately succumbed to IFN-γ-activated host cell innate immunity. Our findings uncover a key role for GRA12 in mediating resistance to host IFN-γ and reveal that many other IVN membrane-associated GRA proteins, as well as PVM-localized GRA proteins, play important roles in establishing chronic infection.IMPORTANCEToxoplasma gondiicysts reactivate during immune deficiency and cause fatal encephalitis. Parasite molecules that coordinate the development of acute and chronic infection are poorly characterized. Here, we show that many intravacuolar network membrane and parasitophorous vacuole membrane-associated dense granule (GRA) proteins orchestrate the development of chronic cystsin vivo. A subset of these GRA proteins also modulate acute virulence, and one protein that associates with the intravacuolar network membranes, namely GRA12, was identified as a major virulence factor required for parasite resistance to host gamma interferon (IFN-γ). Our results revealed that many parasitophorous vacuole membrane and intravacuolar network membrane-associated GRA proteins are essential for successful chronic infection.


Development ◽  
1988 ◽  
Vol 104 (4) ◽  
pp. 533-548 ◽  
Author(s):  
B. Fouquet ◽  
H. Herrmann ◽  
J.K. Franz ◽  
W.W. Franke

A Xenopus laevis mRNA encoding a cytokeratin of the basic (type II) subfamily that is expressed in postgastrulation embryos was cDNA-cloned and sequenced. Comparison of the deduced amino acid sequence of this polypeptide (513 residues, calculated mol. wt 55,454; Mr approximately 58,000 on SDS-PAGE) with those of other cytokeratins revealed its relationship to certain type II cytokeratins of the same and other species, but also remarkable differences. Using a subclone representing the 3′-untranslated portion of the 2.4 kb mRNA encoding this cytokeratin, designated XenCK55(5/6), in Northern blot experiments, we found that it differs from the only other Xenopus type II cytokeratin known, i.e. the simple epithelium-type component XenCK1(8), in that it is absent in unfertilized eggs and pregastrulation embryos. XenCK55(5/6) mRNA was first detected at gastrulation (stage 11) and found to rapidly increase during neurulation and further development. It was also identified in Xenopus laevis cultured kidney epithelial cells of the line A6 and in the adult animal where it is a major polypeptide in the oesophageal mucosa but absent in most other tissues examined. The pattern of XenCK55(5/6) expression during embryonic development was similar to that reported for the type I polypeptides of the ‘XK81 subfamily’ previously reported to be embryo-specific and absent in adult tissues. Therefore, we used a XK81 mRNA probe representing the 3′-untranslated region in Northern blots, S1 nuclease and hybrid-selection-translation assays and found the approximately 1.6 kb XK81 mRNA and the resulting protein of Mr approximately 48,000 not only in postgastrula embryos and tadpoles but also in the oesophagus of adult animals. Our results show that both these type II and type I cytokeratins are synthesized only on gastrulation and are very actively produced in early developmental stages but is continued in at least one epithelium of the adult organism. These observations raise doubts on the occurrence of Xenopus cytokeratins that are strictly specific for certain embryonic or larval stages and absent in the adult. They rather suggest that embryonically expressed cytokeratins are also produced in some adult tissues, although in a restricted pattern of tissue and cell type distribution.


Genome ◽  
2018 ◽  
Vol 61 (3) ◽  
pp. 187-194 ◽  
Author(s):  
Yong Zhou ◽  
Lifang Hu ◽  
Lunwei Jiang ◽  
Shiqiang Liu

Metacaspase (MC), a family of caspase-like proteins, plays vital roles in regulating programmed cell death (PCD) during development and in response to stresses in plants. In this study, five MC genes (designated as CsMC1 to CsMC5) were identified in the cucumber (Cucumis sativus) genome. Sequence analysis revealed that CsMC1–CsMC3 belong to type I MC proteins, while CsMC4 and CsMC5 are type II MC proteins. Phylogenetic tree and conserved motif analysis of MC proteins indicated that these proteins can be classified into two groups, which are correlated with the types of these MC proteins. Gene structure analysis demonstrated that type I CsMC genes contain 4–7 introns, while all type II CsMC genes harbor one intron. In addition, many hormone-, stress-, and development-related cis-elements were identified in the promoter regions of CsMC genes. Expression analysis using RNA-seq data revealed that CsMC genes have distinct expression patterns in various tissues and developmental stages. qRT-PCR results showed that the transcript levels of CsMC genes could be regulated by various abiotic stresses such as NaCl, PEG, and cold. These results demonstrate that the cucumber MC gene family may function in tissue development and plant stress responses.


2021 ◽  
Vol 2 (2) ◽  
pp. 507-534
Author(s):  
Raphael Portmann ◽  
Michael Sprenger ◽  
Heini Wernli

Abstract. The aim of this study is to explore the nature of potential vorticity (PV) cutoff life cycles. While climatological frequencies of such near-tropopause cyclonic vortices are well known, their life cycle and in particular their three-dimensional evolution is poorly understood. To address this gap, a novel method is introduced that uses isentropic air parcel trajectories to track PV cutoffs as three-dimensional objects. With this method, we can distinguish the two fundamentally different PV cutoff lysis scenarios on isentropic surfaces: complete diabatic decay vs. reabsorption by the stratospheric reservoir. This method is applied to the ERA-Interim dataset (1979–2018), and the first global climatology of PV cutoffs is presented that is independent of the selection of a vertical level and identifies and tracks PV cutoffs as three-dimensional features. More than 150 000 PV cutoff life cycles are identified and analyzed. The climatology confirms known frequency maxima of PV cutoffs and identifies additional bands in subtropical areas in the summer hemispheres and a circumpolar band around Antarctica. The first climatological analysis of diabatic decay and reabsorption shows that both scenarios occur equally frequently – in contrast to the prevailing opinion that diabatic decay dominates. Then, PV cutoffs are classified according to their position relative to jet streams (equatorward (Type I), between two jets (Type II), and poleward (Type III)). A composite analysis shows distinct dynamical scenarios for the genesis of the three types. Type I forms due to anticyclonic Rossby wave breaking above subtropical surface anticyclones and hardly results in precipitation. Type II results from anticyclonic Rossby wave breaking in mid-latitudes in regions with split-jet conditions and is frequently accompanied by surface cyclogenesis and substantial precipitation. Type III cutoffs preferentially form due to cyclonic Rossby wave breaking within extratropical cyclones in the storm track regions. We show that important track characteristics (speed, travel distance, frequency of decay and reabsorption, isentropic levels) differ between the categories, while lifetime is similar in all categories. Finally, 12 PV cutoff genesis regions in DJF and JJA are selected to study the regional characteristics of PV cutoff life cycles. As a particularly novel aspect, the vertical evolution of PV cutoffs along the life cycle is investigated. We find that, climatologically, PV cutoffs reach their maximum vertical extent about one day after genesis in most regions. However, while in some regions PV cutoffs rapidly disappear at lower levels by diabatic decay, they can grow downward in other regions. In addition, regional differences in lifetimes, the frequencies of diabatic decay and reabsorption, and the link to surface cyclones are identified that cannot be explained only by the preferred regional occurrence of the different cutoff types as defined above. Finally, we also show that in many regions PV cutoffs can be involved in surface cyclogenesis even after their formation. This study is an important step towards quantifying fundamental dynamical characteristics and the surface impacts of PV cutoffs. The proposed classification according to the jet-relative position provides a useful way to improve the conceptual understanding of PV cutoff life cycles in different regions of the globe. However, these life cycles can be substantially modified by specific regional conditions.


1997 ◽  
Vol 157 (1) ◽  
pp. 97-103 ◽  
Author(s):  
P. S. Leung ◽  
H. C. Chan ◽  
L. X. M. Fu ◽  
P. Y. Leung ◽  
S. B. C. Chew ◽  
...  

2021 ◽  
Author(s):  
Raphael Portmann ◽  
Michael Sprenger ◽  
Heini Wernli

<p>The aim of this study is to explore the nature of potential vorticity (PV) cutoff life cycles. While climatological frequencies of such upper-level cyclonic vortices are well known, their life cycle and in particular their three-dimensional evolution is poorly understood. To address this gap, a novel method is introduced that uses isentropic air parcel trajectories to track PV cutoffs as three-dimensional objects. With this method, we can distinguish the two fundamentally different PV cutoff lysis scenarios on isentropic surfaces: complete diabatic decay vs. reabsorption by the stratospheric reservoir.</p><p>This method is applied to the ERA-interim dataset (1979-2018) and the first global climatology of PV cutoffs is presented that is independent of the selection of a vertical level and identifies and tracks PV cutoffs as three-dimensional features. More than 140’000 PV cutoff life cycles are identified and analyzed. The climatology confirms known frequency maxima of PV cutoffs and identifies additional bands in subtropical areas in the summer hemispheres and a circumpolar band around Antarctica. The first climatological analysis of diabatic decay and reabsorption shows that both scenarios occur equally frequently – in contrast to the prevailing opinion that diabatic decay dominates.</p><p>Further, PV cutoffs are classified according to their position relative to jet streams [equatorward (type I), between two jets (type II), and poleward (type III)]. A composite analysis of PV cutoff genesis shows distinct dynamical scenarios for the three types. Type I forms due to anticyclonic Rossby wave breaking above subtropical surface anticyclones and hardly results in precipitation. Type II results from anticyclonic Rossby wave breaking downstream of the storm tracks and is frequently accompanied by surface cyclogenesis and substantial precipitation. Type III cutoffs preferentially form due to wave breaking within mature extratropical cyclones in the storm track regions. We show that important track characteristics (speed, travel distance, frequency of decay and reabsorption, vertical evolution) differ between the categories, while lifetime is similar in all categories. </p><p>Finally, twelve PV cutoff genesis regions in DJF and JJA are selected to study the regional characteristics of PV cutoff life cycles. We find that many characteristics of these PV cutoffs reflect the preferred regional occurrence of the different life cycle types. However, a few regions are characterized by substantially longer (e.g. central subtropical North Atlantic in summer) or shorter (Mediterranean in summer) lifetimes than most other regions. Furthermore, a remarkable variability in the vertical evolution of PV cutoffs is found. While in some regions, PV cutoffs rapidly disappear at lower levels by diabatic decay, they can grow downward in other regions. We also show that in many regions PV cutoffs can be involved in surface cyclogenesis even after their formation.</p><p>This study is an important step towards quantifying fundamental dynamical characteristics and the surface impacts of PV cutoffs. The proposed classification according to the jet-relative position provides a useful way to improve the conceptual understanding of PV cutoff life cycles. However, these life cycles can be substantially modified by specific regional conditions.</p>


2009 ◽  
Vol 77 (12) ◽  
pp. 5380-5388 ◽  
Author(s):  
Jason P. Gigley ◽  
Barbara A. Fox ◽  
David J. Bzik

ABSTRACT C57BL/6 (B6) mice are genetically highly susceptible to chronic type II Toxoplasma gondii infections that invariably cause lethal toxoplasmic encephalitis. We examined the ability of an attenuated type I vaccine strain to elicit long-term immunity to lethal acute or chronic type II infections in susceptible B6 mice. Mice immunized with the type I cps1-1 vaccine strain were not susceptible to a lethal (100-cyst) challenge with the type II strain ME49. Immunized mice challenged with 10 ME49 cysts exhibited significant reductions in brain cyst and parasite burdens compared to naive mice, regardless of the route of challenge infection. Remarkably, cps1-1 strain-immunized B6 mice chronically infected with ME49 survived for at least 12 months without succumbing to the chronic infection. Potent immunity to type II challenge infections persisted for at least 10 months after vaccination. While the cps1-1 strain-elicited immunity did not prevent the establishment of a chronic infection or clear established brain cysts, cps1-1 strain-elicited CD8+ immune T cells significantly inhibited recrudescence of brain cysts during chronic ME49 infection. In addition, we show that uracil starvation of the cps1-1 strain induces early markers of bradyzoite differentiation. Collectively, these results suggest that more effective immune control of chronic type II infection in the genetically susceptible B6 background is established by vaccination with the nonreplicating type I uracil auxotroph cps1-1 strain.


Author(s):  
Ronald S. Weinstein ◽  
N. Scott McNutt

The Type I simple cold block device was described by Bullivant and Ames in 1966 and represented the product of the first successful effort to simplify the equipment required to do sophisticated freeze-cleave techniques. Bullivant, Weinstein and Someda described the Type II device which is a modification of the Type I device and was developed as a collaborative effort at the Massachusetts General Hospital and the University of Auckland, New Zealand. The modifications reduced specimen contamination and provided controlled specimen warming for heat-etching of fracture faces. We have now tested the Mass. General Hospital version of the Type II device (called the “Type II-MGH device”) on a wide variety of biological specimens and have established temperature and pressure curves for routine heat-etching with the device.


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