Scanning Electron Microscopy of the Outer Taste Pore from a Human Tongue

1969 ◽  
Vol 27 ◽  
pp. 42-43
Author(s):  
I. Kaufman Arenberg ◽  
W. F. Marovitz ◽  
A. P. Mackenzle
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Molecular Sieve ◽  
Freeze Drying ◽  
Osmic Acid ◽  
Chemical Fixation ◽  
Human Tongue ◽  
Neutral Formalin ◽  
Surface Observations ◽  
Scanning Electron

Increasing use is now being made of the scanning electron microscope for soft biologic tissues. The use of chemical fixation followed by freeze-drying, facilitates surface observations of tissues which otherwise would not be possible.Four hours following necropsy a sample of human tongue was removed. This specimen was fixed in 10% neutral formalin, postfixed in 1% osmic acid, dehydrated in graded series of alcohol, and evaporated, in vacuo, over a Linde molecular sieve at -30 ± 1°C. The specimen was plated with gold: palladium (40: 60) in a vacuum evaporator to an approximate 200 Å thickness. The emissive mode of the Cambridge Stereoscan Mark II scanning electron microscope was used for this study.

Human Stapes Crura: Normal Ultrastructure. Scanning Electron Microscopic Findings

1975 ◽  
Vol 33 ◽  
pp. 528-529
Author(s):  
M.D. Graham
Keyword(s):  
Electron Microscope ◽  
Surgical Treatment ◽  
Scanning Electron Microscope ◽  
Osmic Acid ◽  
Human Cadaver ◽  
Electron Microscopic ◽  
Scanning Electron Microscopic ◽  
Normal Human ◽  
Microscopic Findings ◽  
Scanning Electron

The recent development of the scanning electron microscope has added great impetus to the study of ultrastructural details of normal human ossicles. A thorough description of the ultrastructure of the human ossicles is required in order to determine changes associated with disease processes following medical or surgical treatment.Human stapes crura were obtained at the time of surgery for clinical otosclerosis and from human cadaver material. The specimens to be examined by the scanning electron microscope were fixed immediately in the operating room in a cold phosphate buffered 2% gluteraldehyde solution, washed with Ringers, post fixed in cold 1% osmic acid and dehydrated in graded alcohol. Specimens were transferred from alcohol to a series of increasing concentrations of ethyl alcohol and amyl acetate. The tissue was then critical point dried, secured to aluminum stubs and coated with gold, approximately 150A thick on a rotating stage in a vacuum evaporator. The specimens were then studied with the Kent-Cambridge S4-10 Scanning Electron Microscope at an accelerating voltage of 20KV.

Microencapsulation Exploration of Squalene by Ultrasonic Spraying and Freeze-Drying

2012 ◽  
Vol 554-556 ◽  
pp. 1835-1840 ◽  
Author(s):  
Lu Lu Han ◽  
Liang Wu Bi ◽  
Zhen Dong Zhao ◽  
Ya Li Xing
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Freeze Drying ◽  
Suitable Condition ◽  
Sucrose Ester ◽  
Arabic Gum ◽  
Reasonable Condition ◽  
Running Time ◽  
Ultrasonic Emulsification ◽  
Scanning Electron

Squalene, a natural antioxidant, was microencapsulated by the method of ultrasonic spraying and freeze-drying using shell materials of gelatin and arabic gum. The suitable condition for ultrasonic emulsification before ultrasonic spraying and freeze-drying was frequency 28 kHz and running time 20 min. The reasonable condition for microencapsulation of squalene by ultrasonic spraying and freeze-drying was gelatin 3.0 %, arabic gum 5.0 %, maltodextrin 6.0 % and sucrose ester 0.9 %. The appearance and size of microcapsules of squalene were measured by scanning electron microscope and metallographic microscope. The microcapsules of squalene were smonth spheres at the diameter of about 10 μm.

scholarly journals Efektivitas Zeolit Alam Ende-NTT sebagai Adsorben dalam Pemurnian Bioetanol Berbahan Baku Moke: Minuman Tradisional Flores

2020 ◽  
Vol 4 (2) ◽  
pp. 121
Author(s):  
Yulius Dala Ngapa ◽  
Jumilah Gago
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Molecular Sieve ◽  
Natural Zeolite ◽  
Alternative Energy ◽  
Adsorption Process ◽  
Activation Process ◽  
X Ray Diffraction ◽  
X Ray ◽  
Scanning Electron

Ketergantungan akan pemakaian bahan bakar fosil yang terus meningkat telah mengubah pola pikir manusia untuk mengembangkan penelitian terkait energi alternatif terbarukan. Bioetanol yang berasal dari hasil fermentasi nira tanaman aren (Arenga pinnata MERR), dan dikenal sebagai minuman tradisional Moke di Flores dapat dimanfaatkan sebagai bahan bakar alternatif jika memiliki kemurnian di atas 99,5%. Proses adsorpsi merupakan salah satu metode yang dilakukan untuk menghasilkan bioetanol dengan kadar di atas titik azeotrop. Adsorben yang efektif digunakan sebagai molecular sieve adalah zeolit alam yang berasal dari kabupaten Ende – NTT. Peningkatan efisiensi penyerapan zeolit alam dalam pemurnian bioetanol berbahan baku Moke sudah dilakukan melalui proses aktivasi kimia. Penelitian ini bertujuan untuk mengetahui kadar dan kualitas bioetanol setelah pemurnian menggunakan adsorben zeolit alam Ende – NTT yang telah diaktivasi NaOH pada berbagai konsentrasi. Kadar bioetanol yang terdapat pada Moke sebelum dan sesudah proses adsorpsi ditentukan dengan instrumen gas kromatografi, karakterisasi zeolit alam menggunakan X-Ray Diffraction (XRD) dan Scanning Electron Microscope (SEM). Zeolit alam Ende dapat digunakan sebagai adsorben dalam meningkatkan kadar bioetanol berbahan baku Moke.The reliance on increasing use of fossil fuels has changed the human mindset to develop research related to renewable alternative energy. Bioethanol derived from fermented palm sugar sap (Arenga pinnata MERR), and known as Moke, traditional drink in Flores can be used as an alternative fuel if it has a purity above 99.5%. The adsorption process is one method used to produce bioethanol with a level above the azeotrope point. The effective adsorbent used as molecular sieve is natural zeolite originating from Ende - NTT. Increased efficiency of absorption of natural zeolite in bioethanol purification made from Moke has been done through a chemical activation process. This study aims to determine the level and quality of bioethanol after purification using Ende-NTT natural zeolite adsorbent which has been activated by NaOH at various concentrations. Bioethanol levels found in the Moke before and after the adsorption process were determined by gas chromatography instruments, natural zeolite characterization using X-Ray Diffraction (XRD) and Scanning Electron Microscope (SEM). Ende natural zeolite can be used as an adsorbent to increase the level of bioethanol made from Moke.

A technique for preparing Beauveria spp. for scanning electron microscopy

1980 ◽  
Vol 58 (15) ◽  
pp. 1700-1703 ◽  
Author(s):  
E. C. Quattlebaum ◽  
G. R. Carner
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Freeze Drying ◽  
Osmium Tetroxide ◽  
Air Drying ◽  
Preparation Methods ◽  
Critical Point Drying ◽  
Scanning Electron

Vapor fixation for 96 h with 1% osmium tetroxide (OsO4) and 3–4 days air drying produced distortion-free specimens of Beauveria spp. for examination with the scanning electron microscope. A combination of 4 h OsO4 vapor fixation and freeze-drying also reduced disruption satisfactorily but specimens were not as well preserved as with the first method. Preparation methods that were ineffective in preventing collapse of hydrophilic structures were Cling Free® sprayed on specimens prior to examination, freeze-drying, critical-point drying (of unfixed material), and vapor fixation with glutaraldehyde.

Preliminary Study in Cross-Linked Gelatin/Alginate Sponges

2012 ◽  
Vol 184-185 ◽  
pp. 1102-1105
Author(s):  
Ching Wen Lou ◽  
Ming Sheng Huang ◽  
Chiung Yun Chang ◽  
Chao Tsang Lu ◽  
Wen Cheng Chen ◽  
...  
Keyword(s):  
Weight Loss ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Freeze Drying ◽  
Tissue Fluid ◽  
Fluid Absorption ◽  
Degradation Tests ◽  
Swelling Rate ◽  
Preliminary Study ◽  
Scanning Electron

Gelatin and alginate are commonly used as biomaterials for making dressings because both of which have hemostasis and high tissue fluid absorption ability. In this study, gelatin and alginate are mixed at various weight ratios, the mixtures of which then undergo the freeze-drying, forming the gelatin/alginate sponges. The resulting sponges serve as the contact surface of dressings, and swelling and degradation tests evaluate the samples in terms of hydrophilicity and absorbility. The pores in the sponges are then observed by a scanning electron microscope (SEM). According to our study, with a decrease in the amount of gelatin, the determined pore size and swelling rate both increase, but the weight loss decreases.

Bifunctional Pt-Molecular Sieve Catalysts for Hydroisomerization of Long-Chain Olefins

2011 ◽  
Vol 418-420 ◽  
pp. 2250-2254
Author(s):  
Fei Ding ◽  
Cai Rong Luo ◽  
Hai Rong Zhang ◽  
Lian Xiong ◽  
Xin De Chen
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Molecular Sieves ◽  
Molecular Sieve ◽  
Textural Properties ◽  
Product Distribution ◽  
Bifunctional Catalysts ◽  
Long Chain ◽  
Scanning Electron

Bifunctional Pt/HZSM-5, Pt/HBeta, Pt/HMCM-41 and Pt/HSAPO-11 catalysts were prepared using pseudoboehmite as a binder. The H2-TPR profiles, scanning electron microscope (SEM) and brunauer-emmett-teller (BET) pointed out that reducibility of catalysts and textural properties. These bifunctional catalysts have been tested in long-chain olefins hydroisomerization. The experiments were carried out at 320oC under 2.0 MPa of hydrogen and at 3.0 h-1WHSV. Complete hydrogenation of long-chain olefins was observed over Pt/HMCM-41 and Pt/HSAPO-11. Hydroisomerization reactions also occured in the case of Pt supported strong acidic molecular sieves (HZSM-5 and HBeta). The result suggests product distribution of long-chain olefins hydroisomerization is dependent on the balance between the acid and metallic functions in catalysts.

scholarly journals Fabrication of Sponges from Amnion Hydrogel to Apply as Wound Dressing

2021 ◽  
Vol 18 (20) ◽  
pp. 36
Author(s):  
Nghia Thi Hieu Phan ◽  
My Thi Ngoc Nguyen ◽  
Ha Le Bao Tran
Keyword(s):  
Tensile Strength ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Wound Dressing ◽  
Freeze Drying ◽  
Mechanical Test ◽  
Drying Methods ◽  
Relative Growth Rates ◽  
Type Of Injury ◽  
Scanning Electron

A burn is a type of injury to the skin or other tissues. It can give rise to skin defects or even death. In this study, sponges derived from amnion hydrogel were generated to apply as a wound dressing. The sponges were created by combining crosslinking and freeze-drying methods. There were three types of the obtained sponges: MGA-0 (only washed with PBS), MGA-1 (washed in glycine 1 % for one day) and MGA-2 (washed in glycine 1 % for two days). These sponges were evaluated by scanning electron microscope (SEM), mechanical test, swelling, and cytotoxicity. The tensile strength of the sponges was about 1.8 MPa, and the absorption increased during 24 h. The relative growth rates (%RGR) of MGA-0, MGA-1, and MGA-2 were 88.8, 58.2 and 67.2 %, respectively. The obtained results suggested that the MGA-0 sponge has potential for wound dressing application. HIGHLIGHTS The sponges have been manufactured from human amnion hydrogel by combining crosslinking and freeze-drying methods Through the scanning electron microscope (SEM), the surface of the obtained sponges showed a fibrous-like structure These sponges have good absorbency, the tensile strength of the sponges is about 1.8 MPa like the tensile strength of human skin, and the MGA-0 sponge is not cytotoxic GRAPHICAL ABSTRACT

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