Ultrastructure of Oviduct Epithelium of the Porcine in Estrus

1971 ◽  
Vol 29 ◽  
pp. 512-513
Author(s):  
R. K. Nayak ◽  
D. R. Zimmerman
Keyword(s):  
Intercellular Junctions ◽  
Uranyl Acetate ◽  
Secretory Process ◽  
Osmic Acid ◽  
Electron Microscopic ◽  
Thin Sections ◽  
Tissue Sections ◽  
Oviduct Epithelium ◽  
Microscopic Studies ◽  
Cyclic Changes

Cyclic changes of porcine oviduct epithelium studied by light microscopy were first reported by Snyder in 1923. UltrastructuraI features of the porcine oviduct epithelium have not yet been described. Electron microscopic studies of oviduct epithelium have been reported for only a few species. These reports have been recently reviewed by Nilsson and Relnius. This report describes the fine structure of, the oviduct epithelium and attempts to elucidate the mechanism of ciliogenesis and the secretory process in the porcine during estrus.Tissue sections from the fimbria and ampulla were fixed in cold 3% cacodylate buffered glutaraldehyde (pH 7.4), post-fixed in 1% osmic acid and embedded in Epon. Ultra-thin sections were stained with uranyl acetate and lead citrate and examined in a RCA 3-G electron microscope operated at 100 kv.The epithelium of the tubal mucosa consists of secretory and ciliated cells. The cells are columnar and rest on a common basement membrane, which is about 50 mμ thick. The distal or free borders of the surface epithelial cells possess few irregular microvilli. The membranes of adjacent cells show tight intercellular junctions and macula adhaerentes (Figs. 1, 2).

Hemoglobin crystals of the red blood cells in the human renal cortex: electron microscopic observations of the renal lithiasis

1978 ◽  
Vol 36 (2) ◽  
pp. 480-481
Author(s):  
Kosuke Ueda ◽  
Hiroto Washida ◽  
Nakazo Watari
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Renal Cortex ◽  
Uranyl Acetate ◽  
Osmic Acid ◽  
Renal Lithiasis ◽  
Electron Microscopic ◽  
Hemoglobin C ◽  
First Case ◽  
Ultrathin Sections

IntroductionHemoglobin crystals in the red blood cells were electronmicroscopically reported by Fawcett in the cat myocardium. In the human, Lessin revealed crystal-containing cells in the periphral blood of hemoglobin C disease patients. We found the hemoglobin crystals and its agglutination in the erythrocytes in the renal cortex of the human renal lithiasis, and these patients had no hematological abnormalities or other diseases out of the renal lithiasis. Hemoglobin crystals in the human erythrocytes were confirmed to be the first case in the kidney.Material and MethodsTen cases of the human renal biopsies were performed on the operations of the seven pyelolithotomies and three ureterolithotomies. The each specimens were primarily fixed in cacodylate buffered 3. 0% glutaraldehyde and post fixed in osmic acid, dehydrated in graded concentrations of ethanol, and then embedded in Epon 812. Ultrathin sections, cut on LKB microtome, were doubly stained with uranyl acetate and lead citrate.

Cytoplasmic Invaginations in Trophoblasts and Epithelial Cells of Rat

1971 ◽  
Vol 29 ◽  
pp. 524-525
Author(s):  
Iracema M. Baccarini
Keyword(s):  
Osmium Tetroxide ◽  
Uranyl Acetate ◽  
Nuclear Inclusions ◽  
Mucous Cells ◽  
Electron Microscopic ◽  
Cervical Epithelium ◽  
Intact Membrane ◽  
Microscopic Studies ◽  
Abnormal Cells ◽  
Nuclear Features

Some morphological nuclear features (invaginations) in normal and abnormal cells have been described in several electron microscopic studies. They have been referred to by others as blebs, loops, pockets, sheets, bodies, nuclear inclusions and cytoplasmic invaginations. Identical appearing structures were found in cells of the uterine cervical epithelium, in trophoblasts of blastocysts and in trophoblasts of rat placenta.Methods. Uterine cervix (normal rats), rat placenta (9-10 days gestation) and blastocyst were placed in 3% glutarahdehyde for 3 hours. The tissue was washed in phosphate buffer for 24 hours, postfixed in 1%. buffered osmium tetroxide for 1-2 hours and embedded in epon araldite. Sections were double stained with uranyl acetate and lead citrate and viewed in E. M. Siemens 200.Observations. Nuclear invaginations were found in basal, parabasal and mucous cells of the cervix epithelium, in trophoblasts of blastocyst and in trophoblasts of placenta. An oval, round or elongated invagination contained heterogenously cytoplasm surrounded by a double intact membrane; usually several invaginations were found in the same nucleus.

An Electron Microscopic Study of the Acute Effects of Hypothalamic Releasing Factors on the Anterior Pituitary Gland

1968 ◽  
Vol 26 ◽  
pp. 232-233
Author(s):  
P.W. Coates ◽  
E.A. Ashby ◽  
L. Krulich ◽  
A. Dhariwal ◽  
S. McCann
Keyword(s):  
Anterior Pituitary ◽  
Microscopic Investigation ◽  
Uranyl Acetate ◽  
Electron Microscopic Investigation ◽  
Electron Microscopic ◽  
Tissue Samples ◽  
Thin Sections ◽  
Dense Membrane ◽  
Membrane Bound ◽  
Releasing Factors

The morphologic effects on somatotrophs of crude sheep hypothalamic extract prepared from stalk-median eminence were studied by electron microscopy in conjunction with concurrently run bioassays performed on the same tissue samples taken from young adult male Sherman rats.Groups were divided into uninjected controls and injected experimentals sacrificed at 5', 15', and 30' after injection. Half of each anterior pituitary was prepared for electron microscopic investigation, the other half for bioassay. Fixation using collidine buffered osmium tetroxide was followed by dehydration and embedment in Maraglas. Uranyl acetate and lead citrate were used as stains. Thin sections were examined in a Philips EM 200.Somatotrophs from uninjected controls appeared as described in the literature (Fig. 1). In addition to other components, these cells contained moderate numbers of spherical, electron-dense, membrane-bound granules approximately 350 millicrons in diameter.

scholarly journals Preservation of alveolar type II pneumocyte lamellar bodies for electron microscopic studies.

1979 ◽  
Vol 27 (5) ◽  
pp. 989-996 ◽  
Author(s):  
A J Collet
Keyword(s):  
Uranyl Acetate ◽  
Alkaline Solutions ◽  
Alveolar Type ◽  
Morphological Aspect ◽  
Type Ii ◽  
Lamellar Bodies ◽  
Electron Microscopic ◽  
Type Ii Pneumocyte ◽  
Microscopic Studies ◽  
Freeze Etching

Simultaneous fixation with glutaraldehyde and osmium tetroxide, followed by an uranyl acetate (UA) treatment before dehydration and embedding (Hirsch and Fedorko 1968) ensures a very good preservation of lamellar bodies (LB's) as well as of the cellular membranes in type II pneumocyte. The uranyl acetate treatment appeared to be the most efficient step of the procedure. The morphological aspect of lamellar bodies after such a preparation was similar to that observed after freeze-etching of lipid retaining methods. Moreover, the Hirsch-Fedorko procedure is very simple and can easily be used for routine ultrastructural and radioautographic studies. On the other hand, it appeared that the uranyl acetate phospholipid "complex" is very sensitive to the pH of chemical solutions used after sectioning. The "complex" is variously dissolved by alkaline solutions, photographic developers or stains. The best preservation of ultrastructure was obtained with neutral or acidic developers and acidic stains.

Properties of Mouse Leukemia Viruses X V . Electron Microscopic Studies on the Organization of Friend Leukemia Virus and Other Mammalian C-Type Viruses

1978 ◽  
Vol 33 (1-2) ◽  
pp. 124-138 ◽  
Author(s):  
Hermann Frank ◽  
Heinz Schwarz ◽  
Thomas Graf ◽  
Werner Schäfer
Keyword(s):  
Leukemia Virus ◽  
Microscopic Analysis ◽  
Uranyl Acetate ◽  
Viral Envelope ◽  
Preparation Technique ◽  
Electron Microscopic ◽  
Friend Leukemia ◽  
Microscopic Studies ◽  
Detergent Treatment ◽  
Leukemia Viruses

Abstract Further investigations on the structure of Friend murine leukemia virus (FLV) revealed that the transition from the “immature” (now termed “native”) to the structurally less organized “mature” (now termed “collapsed”) form occurs mainly as a result of the preparation for the electron microscope. A short pretreatment of virus with the detergent NP40 prior to negative staining with uranyl acetate is able to preserve the “native” structure of a high percentage of virus particles from standard preparations. Treatment with conventional fixatives was found to be in ­ effective.Using this preparation technique, a more detailed electron microscopic analysis of the viral internal organization became possible. A thin layer designated “inner coat” was newly detected in close apposition to the viral membrane between the viral envelope and core. Removal of the unit membrane by more intensive detergent treatment suggests the existence of material extending be­ tween the viral surface knobs and the viral interior. The icosahedral core shell has an opening at one side and this opening matches with the hole in the apparently beehive-like arranged ribo-nucleoprotein (RNP) strand which represents the innermost structure.A comparative study of a series of representative mammalian C-type viruses with the same technique indicated a close similarity to Friend virus in fine structure, although differences in stability were observed.Based on these and earlier findings a model of the structure of mammalian C-type viruses is presented.

Ultrastructure of Septa from the Filamentous Fungus Aspergillus Nidulans

1998 ◽  
Vol 4 (S2) ◽  
pp. 1142-1143
Author(s):  
Elizabeth A. Richardson ◽  
Michelle Momany
Keyword(s):  
Aspergillus Nidulans ◽  
Filamentous Fungus ◽  
Freeze Substitution ◽  
Uranyl Acetate ◽  
Temperature Sensitive ◽  
Electron Microscopic ◽  
Genetic Studies ◽  
Ultrastructural Studies ◽  
Microscopic Studies ◽  
Dialysis Membranes

The filamentous fungus Aspergillus nidulans partitions its cells by laying down septa at regularly spaced intervals in response to nuclear division. Physiological and genetic studies of the temperature-sensitive sep mutants have been especially useful in dissecting the regulation of septation. Electron microscopic studies of the sep mutants should be equally useful in dissecting the structural intermediates of septation. In preparation for ultrastructural studies of the sep mutants, we have examined septa in wild-type A. nidulans fixed by freeze substitution.Dialysis membranes were placed on rich medium plates and inoculated with A. nidulans spore suspensions. After 12 hours at 30°C, the dialysis membranes with adhering fungal hyphae were cut into square pieces measuring approximately 5mm on each side. The pieces were plunged into liquid propane and processed according to the procedures of Hoch. Serial sections were cut using a diamond knife and post stained with uranyl acetate and lead citrate.

An Electron Microscopic Investigation of the Pathogenesis of Hemorrhage Induced by Rattlesnake Venom

1974 ◽  
Vol 32 ◽  
pp. 56-57
Author(s):  
Charlotte L. Ownby ◽  
Robert A. Kainer ◽  
Anthony T. Tu
Keyword(s):  
Phosphate Buffer ◽  
Osmium Tetroxide ◽  
Microscopic Investigation ◽  
Uranyl Acetate ◽  
Electron Microscopic Investigation ◽  
Lead Citrate ◽  
Electron Microscopic ◽  
Thin Sections ◽  
Rattlesnake Venom ◽  
Diamondback Rattlesnake

One of the significant changes induced by the injection of rattlesnake (Crotalidae) venom is hemorrhage. Since crotaline antivenin does not prevent such local tissue damage, a more effective treatment of snakebite is needed. To aid in the development of such a treatment the pathogenesis of venom-induced hemorrhae was investigated.Swiss-Webster white mice were injected intramuscularly with Western diamondback rattlesnake (Crotalus atrox) venom. Two minutes after the injection, muscle tissue was obtained by bioosy from the thigh and fixed in 6% glutaraldehyde in Milloniq's phosphate buffer (DH 7.4, 2 hrs., 4°C). After post-fixation in 2% osmium tetroxide in Milloniq's phosphate buffer (pH 7.4, 1hr., 4°C) the tissue was dehydrated routinely in ethanol and embedded in Epon 812. The thin sections were stained with uranyl acetate in methanol and lead citrate then observed with either a Zeiss EM 9A or an Hitachi HS-8 electron microscope.

Problems in Myogenesis Terminology in Electron Microscopy

1967 ◽  
Vol 25 ◽  
pp. 38-39
Author(s):  
P.E. Conen ◽  
J.U. Balis ◽  
C.D. Bell
Keyword(s):  
Electron Microscopy ◽  
Cell Types ◽  
Uranyl Acetate ◽  
Electron Microscopic ◽  
Accurate Identification ◽  
Microscopic Studies ◽  
A Cell ◽  
Lead Hydroxide ◽  
Developing Muscle

Myogenesis in man was studied using muscle from 19 fetuses of 8 to 16 weeks gestation which were processed with standard osmium-Epon or glutaraldehyde-osmium-Epon schedules and sections were stained in uranyl acetate and/or lead hydroxide. Particular emphasis was given during this study to presence of basement membrane and myofilaments as additional aids in classification of cell types present in developing muscle.Electron microscopy permits accurate identification of fibroblasts and early cells of muscle series and has been used in studies of myogenesis in chick, and rat. Light microscopy definitions for premyoblasts and myoblasts, and for myocytes at the myotube and muscle fiber stages of development are difficult to apply to electron microscopic studies without modification. For example the term myoblast was used differently by Tello, Katznelson and Boyd to designate a cell destined to become muscle but not recognizable as a muscle cell.

Ultrastructural studies of cartilage in genetic disorders of skeletal growth

1978 ◽  
Vol 36 (2) ◽  
pp. 668-669
Author(s):  
D. O. Sillence ◽  
D. L. Rimoin ◽  
Ruth Silberberg
Keyword(s):  
Genetic Disorders ◽  
Electron Microscopic Examination ◽  
Uranyl Acetate ◽  
Osmic Acid ◽  
Electron Microscopic ◽  
Skeletal Dysplasias ◽  
Ultrastructural Studies ◽  
Low Viscosity ◽  
Transmission Electron ◽  
Cacodylate Buffer

The human skeletal dysplasias are an heterogeneous group of heritable connective tissue disorders associated with abnormalities in the size and shape of the limbs, trunk and/or skull which frequently result in disproportionate short stature. In recent years it has become apparent that these comprise over 50 distinct conditions with a variety of subtypes distinguished on clinical and radiological grounds. We have investigated the pathogenesis of these conditions in over 100 patients by direct transmission electron microscopic examination of chondro-osseous tissue. Some of the ultrastructural studies have been previously reported.Small biopsies of chondro-osseous junction were collected for electron microscopy from the rib or iliac crest of patients with skeletal dysplasias or from normal controls at the time of surgery. These were cut into small blocks and fixed for one hour in either 5% glutaraldehyde in white's buffer or directly in 1% osmic acid in White's buffer or a modified Karnovsky's fixative, (2. 5% paraformaldehyde, 2. 5% glutaraldehyde, 2. 5mM calcium in cacodylate buffer). Subsequent processing included osmium fixation, block staining with uranyl acetate and embedding in Araldite or Spurr's low viscosity resin (firm composition). Sections were cut with glass knives or diamond knives. The latter produced sections which were much more even in thickness, permitting more consistent appraisal of matrix features.

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