Correlative LM, SEM and TEM of argentaffin cells

1978 ◽  
Vol 36 (2) ◽  
pp. 84-85
Author(s):  
R.G. Frederickson ◽  
D.B. Spagnoli ◽  
P.B. DeNee
Keyword(s):  
Electron Microscopy ◽  
Secondary Electron ◽  
Thin Sections ◽  
Backscattered Electron Imaging ◽  
Selective Staining ◽  
Transmission Electron ◽  
Backscattered Electron ◽  
Electron Imaging ◽  
Different Levels ◽  
Tem Grid

The identification of small numbers of cells containing specific materials or having a particular location in a tissue can be observed cften in light microscopic (IM) sections by selective staining. In contrast, the same cells are often difficult to identify in alternate thin sections on grids by transmission electron microscopy (TEM). Grid bars produce periodic obstructions and consequently prevent the visualization of tissue continuity, making it difficult to identify landmarks. Also, the TEM image of a thin section of tissue does not correlate well with the thicker IM image. This is a matter of interpretation based on the different principles of image formation and the different levels of resolution.The purpose of this paper is to demonstrate a method which allows the same cells identified by IM to be more easily located in thin sections for TEM. This method combines the principles of backscattered electron imaging (BSI), secondary electron imaging (SEI) and heavy metal staining with scanning electron microscopy (SEM).

scholarly journals Routine use of backscattered electron imaging to visualize cytochemical and autoradiographic reactions in semi-thin plastic sections.

1990 ◽  
Vol 38 (3) ◽  
pp. 403-414 ◽  
Author(s):  
A Nanci ◽  
S Zalzal ◽  
C E Smith
Keyword(s):  
Light Microscope ◽  
Cell Structure ◽  
Thin Sections ◽  
Backscattered Electron Imaging ◽  
Cell Compartments ◽  
Tissue Organization ◽  
Transmission Electron ◽  
Backscattered Electron ◽  
Thin Plastic ◽  
Electron Imaging

The scanning electron microscope (SEM) was used to examine cytochemical and autoradiographic reactions in 2-microns semi-thin sections of tissues conventionally fixed and embedded in various resins. The sections were examined using both the secondary and backscatter modes of the SEM at magnifications within the range attainable with the light microscope. Both modes allowed the imaging of phosphatase reaction product using cerium and lead capture, lectin-gold, and immunogold labeling, with and without silver enhancement, and autoradiography. Backscattered electron imaging (BEI), however, provided images with more contrast and structural details. This approach allows examination of large sections, with more contrast and resolution than the light microscope, and visualization of reactions not visible with this instrument. The improved imaging and the simple and conventional preparation of specimens indicate that BEI can be used routinely to examine tissue organization, cell structure, and the content of the various cell compartments with a resolution approaching that of transmission electron microscopy.

The Use of Backscattered Electron Imaging and Transmission Electron Microscopy to Assess Bone Architecture and Mineral Loci: Effect of Intermittent Slow-Release Sodium Fluoride Therapy

1996 ◽  
Vol 2 (2) ◽  
pp. 79-86 ◽  
Author(s):  
Joseph E. Zerwekh ◽  
Dennis Bellotto ◽  
Kenneth S. Prostak ◽  
Herbert K. Hagler ◽  
Charles Y.C. Pak
Keyword(s):  
Electron Microscopy ◽  
Slow Release ◽  
Normal Subjects ◽  
Bone Architecture ◽  
Backscattered Electron Imaging ◽  
Transmission Electron ◽  
Mineral Deposition ◽  
Mineralization Defect ◽  
Backscattered Electron ◽  
Electron Imaging

Backscattered electron imaging (BEI) and transmission electron microscopy (TEM) were used to examine the effects of treatment with intermittent slow-release sodium fluoride (SRNaF) and continuous calcium citrate on bone architecture and crystallinity. Examination was performed in nondecalcified biopsies obtained from patients following up to four years of therapy (placebo or SRNaF) and compared to pretreatment biopsies from each patient, as well as to bone from young, normal subjects. BEI images disclosed increased areas of recent bone formation following fluoride administration. There was no evidence of a mineralization defect in any biopsy and both cortical and trabecular architecture remained normal. TEM analysis demonstrated intrafibrillar platelike crystals and extrafibrillar needlelike crystals for both the pre- and post-treatment biopsies as well as for the bone from young normal subjects. There was no evidence of increased crystal size or of an increase in extrafibrillar mineral deposition. These observations suggest that intermittent SRNaF and continuous calcium therapy exerts an anabolic action on the skeleton not accompanied by a mineralization defect or an alteration of bone mineral deposition. The use of BEI and TEM holds promise for the study of the pathophysiology and treatment of metabolic bone diseases.

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