Effect of Thyroxine and Estrogens on Collagen Synthesis in Rats: A Transmission and Scanning Electron Microscopy Study

Author(s):  
A. Lupulescu ◽  
J.E. Habowsky ◽  
J. Milkintas ◽  
D. Birmingham

Recent light and electron microscopic studies showed that corticosteroids and testosterone induced changes in the structure of fibroblasts and collagen synthesis. Our previous investigations demonstrate that also calcitonin significantly interferes with collagen synthesis and fibroblast growth in wounded rabbits.Conflicting findings were reported by using biochemical and light microscopic studies following estrogens and thyroxine administration. This report concerns some investigations with regard to the role of estradiol and thyroxine on collagen synthesis in rats.Adult male rats were used in these experiments. They were divided in three groups of 10 rats each. A) Thyroxine-treated rats: each rat received intramuscularly (im) 100 μg thyroxine (Synthroid), every other day, for one month. B) Estrogen-treated rats: each rat received 500 μg estradiol dipropionate, every other day, for one month. C) Controls, received only the diluent.

1998 ◽  
Vol 05 (01) ◽  
pp. 423-426 ◽  
Author(s):  
M. Remškar ◽  
Z. Škraba ◽  
F. Cléton ◽  
R. Sanjinés ◽  
F. Lévy

Recently it was found that instability of thin weakly bonded MoS 2 sheets against folding can lead to a growth of hollowed microtubes with several mm lengths, 1–20 μm diameter and less than 0.1 μm wall thickness.1 Bent sheets can directly roll up into the tube or they can produce in their microfolds a turbulent gas flow of transported molecules which promotes a tubelike growth mode. These uncommon stable features of inorganic crystals reveal a great similarity to much smaller carbon fullerences and especially to inorganic ( MoS 2, WS 2) nested fullereness-nanotubes.2 Scanning electron microscopic studies of cylindrical microtubes and twisted microribbons are combined with high resolution electron microscopic observations of stacking faults.


2015 ◽  
Vol 10 (3) ◽  
pp. e993264
Author(s):  
Archana Singh ◽  
Mohammad Wahid Ansari ◽  
C P Singh ◽  
Alok Shukla ◽  
Ramesh Chandra Pant ◽  
...  

2007 ◽  
Vol 122 (5) ◽  
pp. 470-475 ◽  
Author(s):  
P M Moreno ◽  
D H Meseguer

AbstractAims:The purpose of this study was to explore the pathogenic role of bone in sinus surgery, and to determine whether mucosal disease after sinus surgery may be induced by underlying disease within the bone.Material and methods:Twenty-five rabbits were divided into five groups. In group one, wide surgical removal of the maxillary sinus mucosa and creation of a nasoantral window were undertaken. In group two, only nasoantral window creation was undertaken. In group three, the mucosa of the anterior maxillary sinus was removed. In group four, a strip of mucosa around the ostium was removed. Group five was used as a control.Results:After three months, in the groups which had undergone wide surgical removal, the medial sinus walls were observed to be retracted and the inner curtain of the bone disturbed, with alteration of the haversian canal system and osteoclastic bone resorption.Conclusions:After radical sinus surgery, electron microscopic changes were observed in the bony walls, similar to those changes seen in osteomyelitis.


2006 ◽  
Vol 43 (1) ◽  
pp. 68-72 ◽  
Author(s):  
Sheila Tavares Pereira ◽  
Nágila Francinete Costa Secundino ◽  
Ana Cristina Carvalho Botelho ◽  
Valéria Cristina Pinheiro ◽  
Wanderly Pedro Tadei ◽  
...  

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Aris F. Pourlis

The main purpose of this study is to extend the microscopic investigations of the pecten oculi in the quail in order to add some information on the unresolved functional anatomy of this unique avian organ. The pecten oculi of the quail was studied by scanning electron microscopy. Eighteen- to-twenty two highly vascularised accordion-like folds were joined apically by a heavily pigmented bridge of tissue, which holds the pecten in a fanlike shape, widest at the base. The structure of the double layered limiting membrane was recorded. The presence of hyalocytes with macrophage-like appearance was illustrated. It is assumed that the pecten oculi of the quail resembles that of the chicken. Illustrated morphological features of this species may add information on the active physiological role of the pecten. But still, the functional significance of this organ is a matter of controversies.


2006 ◽  
Vol 43 (1) ◽  
pp. 68-72 ◽  
Author(s):  
Sheila Tavares Pereira ◽  
Nágila Francinete Costa Secundino ◽  
Ana Cristina Carvalho Botelho ◽  
Valéria Cristina Pinheiro ◽  
Wanderly Pedro Tadei ◽  
...  

Author(s):  
Grace C.H. Yang

The size and organization of collagen fibrils in the extracellular matrix is an important determinant of tissue structure and function. The synthesis and deposition of collagen involves multiple steps which begin within the cell and continue in the extracellular space. High-voltage electron microscopic studies of the chick embryo cornea and tendon suggested that the extracellular space is compartmentalized by the fibroblasts for the regulation of collagen fibril, bundle, and tissue specific macroaggregate formation. The purpose of this study is to gather direct evidence regarding the association of the fibroblast cell surface with newly formed collagen fibrils, and to define the role of the fibroblast in the control and the precise positioning of collagen fibrils, bundles, and macroaggregates during chick tendon development.


Author(s):  
Venita F. Allison

In 1930, Moore, Hughes and Gallager reported that after castration seminal vesicle epithelial cell atrophy occurred and that cell regeneration could be achieved with daily injections of testis extract. Electron microscopic studies have confirmed those observations and have shown that testosterone injections restore the epithelium of the seminal vesicle in adult castrated male rats. Studies concerned with the metabolism of androgens point out that dihydrotestosterone stimulates cell proliferation and that other metabolites of testosterone probably influence secretory function in certain target cells.Although the influence of androgens on adult seminal vesicle epithelial cytology is well documented, little is known of the effect of androgen depletion and replacement on those cells in aging animals. The present study is concerned with the effect of castration and testosterone injection on the epithelium of the seminal vesicle of aging rats.


Author(s):  
Julie A. Martini ◽  
Robert H. Doremus

Tracy and Doremus have demonstrated chemical bonding between bone and hydroxylapatite with transmission electron microscopy. Now researchers ponder how to improve upon this bond in turn improving the life expectancy and biocompatibility of implantable orthopedic devices.This report focuses on a study of the- chemical influences on the interfacial integrity and strength. Pure hydroxylapatite (HAP), magnesium doped HAP, strontium doped HAP, bioglass and medical grade titanium cylinders were implanted into the tibial cortices of New Zealand white rabbits. After 12 weeks, the implants were retrieved for a scanning electron microscopy study coupled with energy dispersive spectroscopy.Following sacrifice and careful retrieval, the samples were dehydrated through a graduated series starting with 50% ethanol and continuing through 60, 70, 80, 90, 95, and 100% ethanol over a period of two days. The samples were embedded in LR White. Again a graduated series was used with solutions of 50, 75 and 100% LR White diluted in ethanol.


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