Scanning Electron Microscopy of the glomerulus in diabetic BB/S wistar rats

1987 ◽  
Vol 45 ◽  
pp. 730-731
Author(s):  
R. C. Kaufmann ◽  
F. K. Khosho ◽  
K. S. Amankwah
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Glucose Tolerance ◽  
Renal Disease ◽  
Wistar Rats ◽  
Renal Damage ◽  
Diabetic Rats ◽  
Wistar Strain ◽  
Glucose Tolerance Tests ◽  
Scanning Electron

Renal damage secondary to diabetes seems to be related to the severity and duration of the diabetes. In streptozotocin and alloxan-induced diabetic rats, renal disease is found only in those rats that have glycosuria and then only after the glycosuria has been present for many months. In these animals, the longer they have glycosuria, the more severe the renal damage. In our colony of BB/S Wistar rats, animals that are going. to become frankly diabetic demonstrate clinical diabetes before they begin spilling glucose in their urine. After glycosuria develops, the condition of the animals worsens; yet, the glucose tolerance tests(GTT) remain essentially unchanged. The purpose of this investigation was to study the animals' kidneys to discover if lesions are present at the onset of glycosuria and how severe the lesions are.Rats of our BB/S Wistar strain were used the day they developed glycosuria. Similarly aged non-diabetic animals were used as controls.

Surface epithelial ultrastructure of the polycystic ovary in the BB wistar rat

1986 ◽  
Vol 44 ◽  
pp. 252-253
Author(s):  
R. C. Kaufmann ◽  
F. K. Khosho ◽  
K. S. Amankwah
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Wistar Rats ◽  
Polycystic Ovary ◽  
Wistar Rat ◽  
Polycystic Ovaries ◽  
Chemical Diabetes ◽  
Scanning Electron

Diabetes decreases the fertility of females, but the mechanisms are not completely understood. In our investigations, we have found that 13% of the female BB Wistar rats that spontaneously developed chemical diabetes had persistent estrous. In this study the ovaries of these rats were examined by scanning electron microscopy(SEM) and compared to normal-cycling controls as well as to rats that had developed polycystic ovaries(PCO) by exposure to constant 1ight.

A rapid fixation method for transmission and Scanning Electron Microscopy using microwave irradiation

1990 ◽  
Vol 48 (3) ◽  
pp. 760-761
Author(s):  
Yasuaki Hotta ◽  
Masumi Nozaki ◽  
Nobuhiko Honda ◽  
Hiroyuki Kato ◽  
Ying Wei Wang ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Microwave Irradiation ◽  
Phosphate Buffer Solution ◽  
Male Rats ◽  
Fixation Method ◽  
Buffer Solution ◽  
Wistar Strain ◽  
Microwave Fixation ◽  
Scanning Electron

Recently, the validity of microwave irradiation (MWI) for electron microscopy has attracted special interest especially in Japan. Recently, we developed a new maceration method for scanning electron microscopy (microwave maceration) and a rapid polymerization method for tissue embedding using epoxy resin (microwave polymerization). Concerning the tissue fixations using MWI (microwave fixation), there are many problems which are not yet clear. In this study, we intend to reveal the effectiveness and mechanism of microwave fixation for scanning and transmission electron mi c roscopy.Liver and trachea taken from Wistar strain male rats were used in this experiment. The fresh livers and tracheae were cut into small pieces and microwave irradiated using a microwave processor (H2500, Bio-Rad) for 1 or 3 min in 2.5% g1utara 1dehyde (GL) bufferized fixatives regulated at pH 7.3 with 0.1M phosphate buffer solution. To prevent the rising of temperature during MWI, specially designed metal case containing water and ice was used (Fig.1).

Three-dimensional aspects of the structural characteristics and kidney angioarchitecture of adult and aged wistar rats: A scanning electron microscopy study

2013 ◽  
Vol 76 (5) ◽  
pp. 538-544 ◽  
Author(s):  
Cristina De Sousa Bolina ◽  
Regina De Sousa Bolina-Matos ◽  
Paulo Henrique De Matos Alves ◽  
Diego Pulzato Cury ◽  
Adriano Polican Ciena ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Wistar Rats ◽  
Structural Characteristics ◽  
Three Dimensional ◽  
Microscopy Study ◽  
Electron Microscopy Study ◽  
Scanning Electron Microscopy Study ◽  
Scanning Electron

Scanning Electron Microscopy of mouse BALB/C/Cd polycystic renal disease

1987 ◽  
Vol 45 ◽  
pp. 932-933
Author(s):  
E. Keyhani ◽  
W. Penasse
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Fine Structure ◽  
Renal Disease ◽  
Solid Tumor ◽  
Single Layer ◽  
The Other ◽  
Distal Tubules ◽  
Number Of Cells ◽  
Scanning Electron

Mouse BALB/C/Cd exhibited spontaneous transplantable kidney adenocarcinoma which affected both sexes and involved both kidneys. This disease is characterized by plain cysts and cysts with single or multiple polyps to solid tumors at various stages of evolution. The fine structure of solid tumor was previously described.Single or multiple (up to 10) cysts were present throughout the cortex parenchyma. Their sizes ranged from 1 to 8 mm in diameter. The cysts occurred from both proximal and distal tubules and were composed of a single layer of cubic-cylindrical epithelial cells. Most cysts exhibited single or multiple focal areas of epithelial polypoid hyperplasia. Single polyps consisted of accumulations of hyperplasic cells piling up on top of each other (Fig. l). The other form consisted of single or multiple branched polyps with a central vascularized core (Fig. 2). The number of cells forming polyps hyperplasia varied between a dozen to up to several hundreds of cells.

scholarly journals Synthesis, photocatalytic and antidiabetic properties of ZnO/PVA nanoparticles

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Shady M. EL-Dafrawy ◽  
Mahmoud Tarek ◽  
Salem Samra ◽  
Shawky M. Hassan
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Active Catalyst ◽  
Sol Gel ◽  
Diabetic Rats ◽  
Poly Vinyl Alcohol ◽  
X Ray Diffraction ◽  
X Ray ◽  
Visible Irradiation ◽  
Scanning Electron

AbstractA series of ZnO and ZnO/poly(vinyl alcohol) (PVA) catalysts were prepared using sol–gel method. An X-ray diffraction analysis confirmed the existence of the wurtzite ZnO phase, and scanning electron microscopy (SEM) observation revealed the formation of spherical ZnO and ZnO/PVA nanoparticles. The decomposition of methylene blue (MB) and methyl orange (MO) induced by the synthesized pure ZnO and ZnO/PVA nanoparticles was studied under ultraviolet–visible irradiation. Among the catalysts evaluated, ZnO/5PVA was the most active in the decomposition of MB, whereas ZnO/7PVA was the most active catalyst in the decomposition of MO. Moreover, an investigation of the biological activity of pure ZnO and ZnO/PVA indicated that ZnO/5PVA exhibited the best performance in lowering the glucose level in diabetic rats.

Scanning Electron Microscopy of the vagina in diabetic BB/S Wistar rats

1987 ◽  
Vol 45 ◽  
pp. 912-913
Author(s):  
F. K. Khosho ◽  
R. C. Kaufmann ◽  
K. S. Amankwah
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Wistar Rats ◽  
Mammalian Species ◽  
Surface Epithelium ◽  
Vaginal Smears ◽  
The Government ◽  
Government Of Canada ◽  
Scanning Electron

The influence of diabetes on reproductive organs in humans and various other mammalian species, including the laboratory rat, is not well documented. We have previously shown that 13% of our BB/S Wistar rat colony demonstrated persistent estrous on vaginal smears. Spontaneously-diabetic BB Wistar rats of our colony demonstrate persistent diestrous on vaginal smears long before showing glucose in urine. In this report, we describe our findings on the ultrastructure of the vaginal epithelium of the frankly diabetic(FD) rat. The rats which started our colony were obtained from the Animal Resource Division of the Government of Canada.Scanning electron microscopy was used to examine specimens of vaginal surface epithelium with comparisons being made with specimens from nondiabetic controls. Cardiovascular perfusion with a buffered glutaraldehyde solution was used for in vivo fixation of the cervix and vagina.

Pathogenesis of Human Hair Defects

1974 ◽  
Vol 32 ◽  
pp. 12-13
Author(s):  
P.S. Porter ◽  
T. Aoyagi ◽  
R. Matta
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Human Hair ◽  
Standard Techniques ◽  
Scanning Electron

Using standard techniques of scanning electron microscopy (SEM), over 1000 human hair defects have been studied. In several of the defects, the pathogenesis of the abnormality has been clarified using these techniques. It is the purpose of this paper to present several distinct morphologic abnormalities of hair and to discuss their pathogenesis as elucidated through techniques of scanning electron microscopy.

Ultrastructural Analysis of the Salivary Glands of Gromphadorhina Portentosa (Schaum)

1977 ◽  
Vol 35 ◽  
pp. 638-639
Author(s):  
P.J. Dailey
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Salivary Glands ◽  
Secretory Vesicles ◽  
The Past ◽  
Transmission Electron ◽  
Means Of Transmission ◽  
Gromphadorhina Portentosa ◽  
Scanning Electron ◽  
Topographical Relief

The structure of insect salivary glands has been extensively investigated during the past decade; however, none have attempted scanning electron microscopy (SEM) in ultrastructural examinations of these secretory organs. This study correlates fine structure by means of SEM cryofractography with that of thin-sectioned epoxy embedded material observed by means of transmission electron microscopy (TEM).Salivary glands of Gromphadorhina portentosa were excised and immediately submerged in cold (4°C) paraformaldehyde-glutaraldehyde fixative1 for 2 hr, washed and post-fixed in 1 per cent 0s04 in phosphosphate buffer (4°C for 2 hr). After ethanolic dehydration half of the samples were embedded in Epon 812 for TEM and half cryofractured and subsequently critical point dried for SEM. Dried specimens were mounted on aluminum stubs and coated with approximately 150 Å of gold in a cold sputtering apparatus.Figure 1 shows a cryofractured plane through a salivary acinus revealing topographical relief of secretory vesicles.

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