A rapid fixation method for transmission and Scanning Electron Microscopy using microwave irradiation

1990 ◽  
Vol 48 (3) ◽  
pp. 760-761
Author(s):  
Yasuaki Hotta ◽  
Masumi Nozaki ◽  
Nobuhiko Honda ◽  
Hiroyuki Kato ◽  
Ying Wei Wang ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Microwave Irradiation ◽  
Phosphate Buffer Solution ◽  
Male Rats ◽  
Fixation Method ◽  
Buffer Solution ◽  
Wistar Strain ◽  
Microwave Fixation ◽  
Scanning Electron

Recently, the validity of microwave irradiation (MWI) for electron microscopy has attracted special interest especially in Japan. Recently, we developed a new maceration method for scanning electron microscopy (microwave maceration) and a rapid polymerization method for tissue embedding using epoxy resin (microwave polymerization). Concerning the tissue fixations using MWI (microwave fixation), there are many problems which are not yet clear. In this study, we intend to reveal the effectiveness and mechanism of microwave fixation for scanning and transmission electron mi c roscopy.Liver and trachea taken from Wistar strain male rats were used in this experiment. The fresh livers and tracheae were cut into small pieces and microwave irradiated using a microwave processor (H2500, Bio-Rad) for 1 or 3 min in 2.5% g1utara 1dehyde (GL) bufferized fixatives regulated at pH 7.3 with 0.1M phosphate buffer solution. To prevent the rising of temperature during MWI, specially designed metal case containing water and ice was used (Fig.1).

Field Emission Scanning Electron Microscopy Of Mouse Cerebellar Synaptic Contacts

2000 ◽  
Vol 6 (S2) ◽  
pp. 844-845
Author(s):  
O.J. Castejón ◽  
R. P. Apkarian ◽  
H. V. Castejón
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Field Emission ◽  
Cerebellar Cortex ◽  
Osmium Tetroxide ◽  
Phosphate Buffer Solution ◽  
Buffer Solution ◽  
Solution Ph ◽  
Osmium Tetroxide Solution ◽  
Scanning Electron

Samples of albino mice cerebellar cortex were processed by the cryofracture method for scanning electron microscopy and examined with the field emission scanning electron microscope (FESEM). Albino mouse cerebellar cortex was excised, cut into 1-2 mm slices and inmersed in 4% glutaraldehyde in O. l M phosphate buffer solution, pH 7.4, for 24h at 4°C; and postfixed for 1 h in a similarly buffered 1% osmium tetroxide solution. Specimens were dehydrated in a graded serie of ethanol (30, 50, 70, 80, 90 2x100%) prior to wrapping individual tissue pieces in preformed absolute ethanol filled parafilm cryofracture packets. Rapid freezing of packets was performed by plunging into LN2. First, the packet was transferred from the LN2 storage vessel with LNT chilled forceps in order to avoid themial damage. Secondly, the cooled fracture blade was removed from the LN2, the packet was orientated under the blade, and immediately struck with a heavy tool.

scholarly journals Pd-Au Electrocatalysts for Hydrogen Evolution Reaction at Neutral pH

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Elitsa Chorbadzhiyska ◽  
Mario Mitov ◽  
Georgi Hristov ◽  
Nina Dimcheva ◽  
Lori Nalbandian ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Hydrogen Evolution ◽  
Hydrogen Evolution Reaction ◽  
Phosphate Buffer Solution ◽  
Hydrogen Gas ◽  
Mass Spectrometry Analysis ◽  
Carbon Felt ◽  
Buffer Solution ◽  
Spectrometry Analysis ◽  
Scanning Electron

Pd-Au codeposits with different ratio of both metals were electrodeposited on carbon felt, characterized by scanning electron microscopy, and investigated as electrocatalysts towards hydrogen evolution reaction in neutral phosphate buffer solution. The quantities of the produced hydrogen gas with different electrocatalysts, estimated from data obtained by chronoamperometry, were confirmed by mass spectrometry analysis. The highest hydrogen evolution rate was achieved with the electrocatalysts, produced from electrolyte with equal Pd and Au content.

Microwave fixation of cells in culture for scanning electron microscopy

1983 ◽  
Vol 41 ◽  
pp. 630-631
Author(s):  
E.C. Chew ◽  
D.J. Riches ◽  
P.P.L. Tam ◽  
G.S.W. Tsao ◽  
T.K. Lam ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Cell Suspension ◽  
Single Cell ◽  
Culture Medium ◽  
Single Cell Suspension ◽  
Electron Microscopic ◽  
Syringe Needle ◽  
Microwave Fixation ◽  
Scanning Electron

The use of microwave irradiation for fixation of human and animal tissue has been proven satisfactory at light microscopic and electron microscopic levels. The present communication reports the study of the same method of fixation of cell cultures for scanning electron microscopy.Trophoblasts were isolated from the placentae of mouse conceptuses at 10.5 days of gestation. The placenta was dissected out from the decidua and placed in Ca and Mg-free PBS, minced and then forced through a gauge-21 syringe needle. The tissue fragments were digested with 0.25% trypsin in Ca and Mg-free PBS for 20 - 30 minutes at 4°C. The digested tissue was then washed with complete PB1 medium. A single-cell suspension was obtained by spinning down the larger fragments by centrifugation. A known volume of the single-cell suspension was added to the culture medium (DCMEM and 20% FCS). The culture medium was changed after 24 hours to remove any unattached cells.

Scanning Electron Microscopy of the glomerulus in diabetic BB/S wistar rats

1987 ◽  
Vol 45 ◽  
pp. 730-731
Author(s):  
R. C. Kaufmann ◽  
F. K. Khosho ◽  
K. S. Amankwah
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Glucose Tolerance ◽  
Renal Disease ◽  
Wistar Rats ◽  
Renal Damage ◽  
Diabetic Rats ◽  
Wistar Strain ◽  
Glucose Tolerance Tests ◽  
Scanning Electron

Renal damage secondary to diabetes seems to be related to the severity and duration of the diabetes. In streptozotocin and alloxan-induced diabetic rats, renal disease is found only in those rats that have glycosuria and then only after the glycosuria has been present for many months. In these animals, the longer they have glycosuria, the more severe the renal damage. In our colony of BB/S Wistar rats, animals that are going. to become frankly diabetic demonstrate clinical diabetes before they begin spilling glucose in their urine. After glycosuria develops, the condition of the animals worsens; yet, the glucose tolerance tests(GTT) remain essentially unchanged. The purpose of this investigation was to study the animals' kidneys to discover if lesions are present at the onset of glycosuria and how severe the lesions are.Rats of our BB/S Wistar strain were used the day they developed glycosuria. Similarly aged non-diabetic animals were used as controls.

Preparation and Mechanical Properties of Modified Magnesium Oxysulfate Cement Incorporating Alkali Conditioner

2020 ◽  
Vol 12 (10) ◽  
pp. 1558-1567
Author(s):  
Shengbin Li ◽  
Haoqian Ren ◽  
Qin Wu ◽  
Yiyang Ye
Keyword(s):  
Electron Microscopy ◽  
Mechanical Properties ◽  
Scanning Electron Microscopy ◽  
Calcium Carbonate ◽  
Mechanical Test ◽  
Mechanical Tests ◽  
Molar Ratio ◽  
Buffer Solution ◽  
Magnesium Oxysulfate ◽  
Scanning Electron

Magnesium oxysulfate cement is an ecological gas-hardening cementitious material. The cement has a complex system, insufficient hydration, and unstable hydration products, so that the cement mechanical property is poor. In this study, calcium chloride/carbon dioxide/weak buffer solution is used to generate alkaline additive, namely vaterite calcium carbonate. The additive material is characterized by scanning electron microscopy and X-ray diffraction, and then the optimal MgO/MgSO4/H2O molar ratio after calcium carbonate is added to magnesium oxysulfate cement, the optimal laying method and reasonable amount of vaterite calcium carbonate are analyzed by mechanical tests. In the experiment, the basic additive is characterized firstly. It is found that the XRD of the additive mainly includes the peaks of calcite and vaterite. It can be seen by scanning electron microscopy that many calcites and vaterites are not formed, the calcites are accumulated more, and there is a large number of particles, which are not appeared before. In the mechanical test of magnesium sulfide cement, the mechanical properties of magnesium oxysulfate cement will increase firstly and then decrease with the increase of MgO/MgSO4 molar ratio in the way of long-cut calcium carbonate for reinforcement. Compared with the layout of long-cut calcium carbonate-magnesium oxysulfate cement, the layout of short-cut calcium carbonate-magnesium oxysulfate cement can enhance the toughness of the modified cement, increase the amount of calcium carbonate, and improve the flexural strength and toughness index of the modified magnesium oxysulfate cement. The blending ratio is better to be 6%. Based on above researches and demonstrations, blending the calcium carbonate-based alkaline additive can effectively improve the mechanical properties of the magnesium oxysulfate cement.

Scanning electron microscopy of mouse ciliated oviduct and tracheal epithelium infected in vitro with Bordetella pertussis

1983 ◽  
Vol 29 (4) ◽  
pp. 415-420 ◽  
Author(s):  
Lauren B. Opremcak ◽  
Melvin S. Rheins
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Organ Culture ◽  
Bordetella Pertussis ◽  
Tracheal Ring ◽  
Fixation Method ◽  
Bacterial Cells ◽  
Ciliated Cells ◽  
Scanning Electron

Infection of mouse tracheal organ culture with Bordetella pertussis resulted in ciliostasis within 36 h. Scanning electron microscopy revealed that B. pertussis attached exclusively to ciliated cells but did not induce expulsion of this cell type at a test interval of 48 h. Mouse oviduct organ culture infected with B. pertussis demonstrated the same strict tropism for ciliated cells as in the tracheal ring system. Only ciliated cells were parasitized, becoming heavily colonized 48 h postinfection. Infected ciliated oviduct cells were not extruded. A fixation method which enhances fine structure was used in the scanning electron microscope studies. Bacterial fimbriae were not observed as the method of attachment of B. pertussis to cilia but fine fibers were seen extending between cilia and bacterial cells.

Coupled ZnO/TiO2 Nanorods Deposited in the Pores of Expanded Graphite Prepared by Microwave Irradiation

2011 ◽  
Vol 204-210 ◽  
pp. 268-272
Author(s):  
Min Cong Zhu ◽  
En Qiang Wang ◽  
Ying Chen Zhang ◽  
Deng Xin Li
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Microwave Irradiation ◽  
Expanded Graphite ◽  
Hydrolysis Method ◽  
Catalytic Nanoparticles ◽  
Key Factor ◽  
Temperature And Pressure ◽  
Harsh Conditions ◽  
Scanning Electron

Expanded graphite (EG) was prepared by microwave irradiation at 1000W for 60s. The growth of coupled ZnO/TiO2nanonods in the pores of EG by using hydrolysis method without harsh conditions was investigated and their microstructure was studied by scanning electron microscopy (SEM). Results show that pores of EG, which can load catalytic nanoparticles, is the physical base for growth of catalytic nanorods. The active edges of graphene of freshly EG is a key factor for the growth of coupled ZnO/TiO2nanorods under non-catalytic and normal temperature and pressure conditions.

SCANNING ELECTRON MICROSCOPY OBSERVATION OF IN-DEVICE InAs/AlAs QUANTUM DOTS BY SELECTIVE ETCHING OF CAPPING LAYERS

2007 ◽  
Vol 21 (14) ◽  
pp. 859-866
Author(s):  
JIE SUN ◽  
DAYONG ZHOU ◽  
RUOYUAN LI ◽  
CHANG ZHAO ◽  
XIAOLING YE ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Quantum Dots ◽  
Selective Etching ◽  
Buffer Solution ◽  
Device Structure ◽  
Alas Layer ◽  
Tunneling Diode ◽  
Rest Areas ◽  
Scanning Electron

Self-assembled InAs/AlAs quantum dots embedded in a resonant tunneling diode device structure are grown by molecular beam epitaxy. Through the selective etching in a C 6 H 8 O 7 · H 2 O-K 3 C 6 H 5 O 7 · H 2 O-H 2 O 2 buffer solution, 310 nm GaAs capping layers are removed and the InAs/AlAs quantum dots are observed by field-emission scanning electron microscopy. It is shown that as-fabricated quantum dots have a diameter of several tens of nanometers and a density of 1010 cm-2 order. The images taken by this means are comparable or slightly better than those of transmission electron microscopy. The undercut of the InAs/AlAs layer near the edges of mesas is detected and that verifies the reliability of the quantum dot images. The inhomogeneous oxidation of the upper AlAs barrier in H 2 O 2 is also observed. By comparing the morphologies of the mesa edge adjacent regions and the rest areas of the sample, it is concluded that the physicochemical reaction introduced in this letter is diffusion limited.

scholarly journals Evaluation of two fixation techniques for direct observation of biofilm formation of Bacillus subtilis in situ, on Congo red agar, using scanning electron microscopy

2020 ◽  
Vol 13 (6) ◽  
pp. 1133-1137
Author(s):  
Nadia Mahmoud Tawfiq Jebril
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Direct Observation ◽  
Congo Red ◽  
Fixation Method ◽  
Fixation Technique ◽  
Fixation Techniques ◽  
Biofilm Development ◽  
Scanning Electron

Background and Aim: Direct observation, scanning electron microscopy (SEM) is a common method used for the observations of biofilms. N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide) (EDC) fixation method has proven to be a valuable fixation method in the observation of these biofilms. Still, it entails a method of biofilm fixation that can damage slim structures, leading to the impossible observation of biofilm development. In contrast, alcian blue and lysine (ABL) fixation technique appears more glycocalyx of biofilm, fully preserved samples, which may provide much insight into the development of B. subtilis biofilms. Materials and Methods: Here, the evaluation of the fixation of ABL technique for the study of B. subtilis biofilms was carried out in situ, on Congo red agar. In doing so, the comparison to commonly use conventional EDC technique for sample fixation, and observation was carried out. Observations were based on SEM over 30 samples. Results: Overall, ABL technique provided excellent observation of biofilms formed in situ, on Congo red agar, and revealed slime structures, which have not been observed, much in standard EDC fixation or earlier in other studies of these biofilms in B. subtilis. Conclusion: This study reported the appropriate use of ABL in the fixation technique for the preservation of biofilm of B. subtilis.

Sign in / Sign up

Export Citation Format

Share Document