scholarly journals Acridine orange fluorescent microscopy is more sensitive than India ink light microscopy in the rapid detection of cryptococcosis among CrAg positive HIV patients

PLoS ONE ◽  
2017 ◽  
Vol 12 (7) ◽  
pp. e0182108 ◽  
Author(s):  
Richard Kwizera ◽  
Andrew Akampurira ◽  
Darlisha Williams ◽  
David R. Boulware ◽  
David B. Meya ◽  
...  
Keyword(s):  
Light Microscopy ◽  
Acridine Orange ◽  
Rapid Detection ◽  
Fluorescent Microscopy ◽  
Hiv Patients ◽  
India Ink

Microvasculature and structure of hemal lymph nodes in the wall of the rabbit bladder: a vascular corrosion casting and EM study

1995 ◽  
Vol 53 ◽  
pp. 1074-1075
Author(s):  
F.E. Hossler ◽  
M.I. McKamey ◽  
F.C. Monson
Keyword(s):  
Lymph Nodes ◽  
Light Microscopy ◽  
Corrosion Casting ◽  
Fixed Tissue ◽  
Infusion Pressure ◽  
India Ink ◽  
Cacodylate Buffer ◽  
Rabbit Bladder ◽  
Lateral Walls ◽  
Comprehensive Study

A comprehensive study of the microvasculature of the normal rabbit bladder, revealed unusual "capillary glomeruli" along the lateral walls. Here they are characterized as hemal lymph nodes using light microscopy, SEM, TEM, ink injection, and vascular casting.Bladders were perfused via a cannula placed in the abdominal aorta with either 2% glutaraldehyde in 0.1M cacodylate buffer (pH 7.4) for fixation, 10% India ink in 0.9% saline and 0.1M phosphate (pH 7.4) for vessel tracing, or resin (Mercoximethylmethacrylate: catalyst, 4:1:0.3; Ladd Research Industries) for vascular corrosion casting. Infusion pressure was 100mm Hg. Fixed tissue was sectioned from epon-araldyte resin, and stained with toluidine blue for light microscopy, and lead and uranium for TEM. Ink injected tissue was photographed directly from saline-filled bladders illuminated from below. Resin-filled tissue was macerated in 5% KOH and distilled water. Casts were critical point dried, sputter coated with goldpalladium, and examined by routine SEM at 10 KV.

STUDIES ON THE BIOLOGICAL PROPERTIES AND CLASSIFICATION OF SV4 VIRUS

1965 ◽  
Vol 11 (2) ◽  
pp. 325-335 ◽  
Author(s):  
S. A. Sattar ◽  
K. R. Rozee
Keyword(s):  
Electron Microscope ◽  
Rhesus Monkey ◽  
Red Blood Cells ◽  
Acridine Orange ◽  
Blood Cells ◽  
Magnesium Chloride ◽  
Fluorescent Microscopy ◽  
Biological Properties ◽  
Acridine Orange Staining

Cytopathic changes in LLC-MK2 cells infected with SV4 virus, observed with the electron microscope and using acridine orange staining and fluorescent microscopy, have been shown to be similar to that caused by picornaviruses and members of the Columbia-SK virus group. The virus was found to be stabilized against heat in the presence of molar magnesium chloride, and to be stable at pH 3.5. The virus was non-pathogenic for suckling mice, failed to agglutinate sheep and human "O" red blood cells, but agglutinated rhesus monkey erythrocytes at 4 °C. On the basis of these properties and those already known, it was suggested that SV4 virus be placed in the group Enteroviruses of lower animals.

scholarly journals Morphological characterization of the gonads of bullfrog tadpoles (Lithobates catesbeianus)

2015 ◽  
Vol 32 (04) ◽  
pp. 245-252 ◽  
Author(s):  
G. Rizzi ◽  
E. Silva-Zacarin ◽  
C. Oliveira ◽  
M. Costa ◽  
R. Salla ◽  
...  
Keyword(s):  
Light Microscopy ◽  
Acridine Orange ◽  
Morphological Study ◽  
Gonadal Development ◽  
Morphological Characterization ◽  
Lithobates Catesbeianus ◽  
Secondary Spermatocyte ◽  
Gosner Stage

Abstract Introduction: This work describes various aspects of early gonadal development of female and male in pre-metamorphic tadpoles (Lithobates catesbeianus) at Gosner stage 25. Materials and Methods: Ovaries and testicles were prepared for routine light microscopy for morphological study and for acridine orange technique fluroescent microscopy for observation of RNA cytoplasm activity. Results: The results showed that female gonads at Gosner stage 25 predominated primary and secondary oogonias, as well as primary, secondary and tertiary oocytes. The developing testicle presented primary spermatogonia (I or A) and secondary spermatogonia (II or B), and as well as primary and secondary spermatocyte. All this cell phases were morphologically characterized and the cell sizes measured. In pre-metamorphic testes the somniferous duct are not developed and the vasa deferentia is opened. Conclusion: At this point, it was possible differentiate ovary from testes does not for the gonadal cells, but for the general anatomy of the organs, being the ovary a spheroid structure and the testicle an elongated tubule.

scholarly journals Acridine orange for diagnosis of malaria – Our experience

2016 ◽  
Vol 6 (1) ◽  
pp. 49-51
Author(s):  
Neeta Jangale ◽  
Ashwini Waghmare
Keyword(s):  
Acridine Orange ◽  
High Performance ◽  
Fluorescent Microscopy ◽  
Peripheral Blood Smear ◽  
Laboratory Diagnosis ◽  
Buffy Coat ◽  
Acridine Orange Staining ◽  
Qualified Personnel ◽  
Blood Smear Examination ◽  
Set Up

Light microscopy of Romanowsky stained peripheral blood smear examination is the age old and commonest method for laboratory diagnosis of malaria. However it is labor intensive, time consuming and requires qualified personnel. So fluorescent microscopy variation that is Quantitative Buffy Coat method( QBC) came into vogue. But QBC imposes cost limitation despite its high performance. Therefore we decided to evaluate fluorescent method using acridine orange in our set up. In this study we have compared Acridine Orange staining with Leishman’s staining as gold standard. The sensitivity and specificity of acridine orange was 99.28% and 97.19% respectively, while positive predictive value (PPV) and negative predicitive value was 89.93% and 99.82% respectively.South East Asia Journal of Public Health Vol.6(1) 2016: 49-51

Acridine Orange Fluorescent Microscopy and the Detection of Malaria in Populations with Low-Density Parasitemia

1991 ◽  
Vol 44 (1) ◽  
pp. 17-20 ◽  
Author(s):  
Chansuda Wongsrichanalai ◽  
Henry Wilde ◽  
Jaturaporn Pornsilapatip ◽  
Anna Luccini ◽  
Pongpun Pansamdang ◽  
...  
Keyword(s):  
Acridine Orange ◽  
Fluorescent Microscopy ◽  
Low Density

scholarly journals Morphological analysis of the seeds of three pseudocereals by using light microscopy and ESEM-EDS

2020 ◽  
Vol 64 (1) ◽  
Author(s):  
Paolino Ninfali ◽  
Anna Panato ◽  
Federica Bortolotti ◽  
Laura Valentini ◽  
Pietro Gobbi
Keyword(s):  
Light Microscopy ◽  
Seed Coat ◽  
Fluorescent Microscopy ◽  
Chenopodium Quinoa ◽  
Environmental Scanning Electron Microscopy ◽  
Seed Morphology ◽  
Fluorescent Light ◽  
Vascular Bundles ◽  
Processing Technologies ◽  
Structural Resistance

The seed morphology of three Pseudocereal Grains (PSCg), i.e. quinoa (Chenopodium quinoa Willd, Chenopodiaceae), buckwheat (Fagopyrum esculentum Moench, Polygonaceae) and amaranth (Amaranthus caudatus L., Amaranthaceae) was studied by light microscopy (LM) and Environmental Scanning Electron Microscopy coupled with Energy Dispersive Spectroscopy (ESEM-EDS). LM was used with visible light to evaluate either unstained sections or sections stained with Azan mixture and with fluorescent light. The aim of the study was to compare the architecture of the three seeds in order to connect their morphology with nutrient localization. The Azan staining allowed for the visualization of the seed coat, the embryo - with its shoot apical meristem - and the radicle cell layers, whereas the use of fluorescent microscopy identified the cells rich in phenolic compounds. Finally, the ESEM-EDS analysis revealed that the seed coat of the quinoa was thinner than that of amaranth or buckwheat. In all PSCg, starch granules appeared to be located in large polygonal cells, surrounded by a thin cell wall. Several globoids of proteins were observed in the embryo cells. In the radicle section, the vascular bundles of the procambium were evident, while Amaranth only showed a consistent layer of calcium crystals, located between the embryo and the perysperm. The morphological differences of the three PSCg were discussed in the context of their structural resistance to processing technologies which impact on nutritional value of derived foods.

scholarly journals In Vitro Effects of Papaverine on Cell Proliferation, Reactive Oxygen Species, and Cell Cycle Progression in Cancer Cells

Molecules ◽  
2021 ◽  
Vol 26 (21) ◽  
pp. 6388
Author(s):  
Daniella A. Gomes ◽  
Anna M. Joubert ◽  
Michelle H. Visagie
Keyword(s):  
Cell Cycle ◽  
Light Microscopy ◽  
Cell Lines ◽  
Growth Medium ◽  
Cell Cycle Progression ◽  
Fluorescent Microscopy ◽  
A549 Cells ◽  
Du145 Cell ◽  
G1 Phase ◽  
Cycle Progression

Papaverine (PPV) is an alkaloid isolated from the Papaver somniferum. Research has shown that PPV inhibits proliferation. However, several questions remain regarding the effects of PPV in tumorigenic cells. In this study, the influence of PPV was investigated on the proliferation (spectrophotometry), morphology (light microscopy), oxidative stress (fluorescent microscopy), and cell cycle progression (flow cytometry) in MDA-MB-231, A549, and DU145 cell lines. Exposure to 150 μM PPV resulted in time- and dose-dependent antiproliferative activity with reduced cell growth to 56%, 53%, and 64% in the MDA-MB-231, A549, and DU145 cell lines, respectively. Light microscopy revealed that PPV exposure increased cellular protrusions in MDA-MB-231 and A549 cells to 34% and 23%. Hydrogen peroxide production increased to 1.04-, 1.02-, and 1.44-fold in PPV-treated MDA-MB-231, A549, and DU145 cells, respectively, compared to cells propagated in growth medium. Furthermore, exposure to PPV resulted in an increase of cells in the sub-G1 phase by 46% and endoreduplication by 10% compared to cells propagated in growth medium that presented with 2.8% cells in the sub-G1 phase and less than 1% in endoreduplication. The results of this study contribute to understanding of effects of PPV on cancer cell lines.

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