scholarly journals STAINING METHODS APPLICABLE TO SECTIONS OF OSMIUM-FIXED TISSUE FOR LIGHT MICROSCOPY

1961 ◽  
Vol 11 (2) ◽  
pp. 502-506 ◽  
Author(s):  
Bryce L. Munger
Keyword(s):  
Light Microscopy ◽  
Fixed Tissue ◽  
Staining Methods

Microvasculature and structure of hemal lymph nodes in the wall of the rabbit bladder: a vascular corrosion casting and EM study

1995 ◽  
Vol 53 ◽  
pp. 1074-1075
Author(s):  
F.E. Hossler ◽  
M.I. McKamey ◽  
F.C. Monson
Keyword(s):  
Lymph Nodes ◽  
Light Microscopy ◽  
Corrosion Casting ◽  
Fixed Tissue ◽  
Infusion Pressure ◽  
India Ink ◽  
Cacodylate Buffer ◽  
Rabbit Bladder ◽  
Lateral Walls ◽  
Comprehensive Study

A comprehensive study of the microvasculature of the normal rabbit bladder, revealed unusual "capillary glomeruli" along the lateral walls. Here they are characterized as hemal lymph nodes using light microscopy, SEM, TEM, ink injection, and vascular casting.Bladders were perfused via a cannula placed in the abdominal aorta with either 2% glutaraldehyde in 0.1M cacodylate buffer (pH 7.4) for fixation, 10% India ink in 0.9% saline and 0.1M phosphate (pH 7.4) for vessel tracing, or resin (Mercoximethylmethacrylate: catalyst, 4:1:0.3; Ladd Research Industries) for vascular corrosion casting. Infusion pressure was 100mm Hg. Fixed tissue was sectioned from epon-araldyte resin, and stained with toluidine blue for light microscopy, and lead and uranium for TEM. Ink injected tissue was photographed directly from saline-filled bladders illuminated from below. Resin-filled tissue was macerated in 5% KOH and distilled water. Casts were critical point dried, sputter coated with goldpalladium, and examined by routine SEM at 10 KV.

Staining methods for sections of epon-embedded tissues for light microscopy

1970 ◽  
Vol 48 (1) ◽  
pp. 189-191 ◽  
Author(s):  
C. Roland Leeson ◽  
Thomas S. Leeson
Keyword(s):  
Light Microscopy ◽  
Synthetic Resin ◽  
Electron Microscopic ◽  
Glass Slides ◽  
Staining Methods

Sections 0.5–2 μ thick are mounted on clean glass slides and allowed to dry. A number of staining procedures are described. After the sections are stained, permanent preparations are made by mounting them in a synthetic resin. The methods result in sections which are suitable for routine light microscopy and for comparison with adjacent electron microscopic sections.

Cytochemical methods for the rapid demonstration of infection at the tissue-biomaterial interface

1992 ◽  
Vol 50 (1) ◽  
pp. 634-635
Author(s):  
J. Hanker ◽  
J.J. Dobbins ◽  
P.E. Yates ◽  
B.L. Giammara
Keyword(s):  
Light Microscopy ◽  
Culture Techniques ◽  
The Past ◽  
Glass Slides ◽  
Staining Methods ◽  
The Times

Infection can be an extensive problem developing during implantation of an autogenous or prosthetic (biomaterial) device or graft. Although culture techniques are invaluable in identifying the responsible microorganisms, the times required frequently emphasize the need for rapid staining methods which can reduce the classification times from days to hours. Studies in our laboratories over the past few years have resulted in microwave-accelerated stains and in methods developed by Giammara, which enable the rapid study of glass slides and coverslips by electron as well as light microscopy.

scholarly journals EFFECT OF INFLUENZA VIRUS ON CILIA AND EPITHELIAL CELLS IN THE BRONCHI OF MICE

1952 ◽  
Vol 95 (2) ◽  
pp. 173-189 ◽  
Author(s):  
Carl G. Harford ◽  
Alice Hamlin
Keyword(s):  
Influenza Virus ◽  
Viral Infection ◽  
Cellular Proliferation ◽  
Pneumococcal Infection ◽  
Bronchial Epithelium ◽  
Ciliary Activity ◽  
Polymorphonuclear Leucocytes ◽  
Fixed Tissue ◽  
Staining Methods ◽  
Ciliary Beat

In order to determine the effect of infection with influenza virus on bronchial cilia of the mouse, ciliary beat has been visualized directly by microscopic examination of the bronchi in slices of fresh lung. Cilia have been shown also in sections of fixed tissue by the use of special silver staining methods. The results have shown persistence of the cilia in spite of severe viral infection and indicate that the lowered resistance to secondary pneumococcal infection which occurs in influenzal pneumonia of the mouse is not due to interference with the ciliary mechanism. By a process of exclusion, the findings give further support to the theory that lowered resistance to pneumococcal infection in influenzal pneumonia is due to edema fluid in the viral lesion furnishing a culture medium for inhaled pneumococci. A widely used method for evaluation of ciliary activity on respiratory epithelia has been the microscopic observation of wave-like movements in reflected light. This activity was observed readily in the bronchi of mice but evidence was obtained showing that at this site it was due to something other than ciliary beat. Further histopathologic observations were made in order to define the lesion of the bronchial epithelium that would permit sparing of ciliated cells. In addition to usual techniques, mice were injected with colchicine for estimation of the rate of cellular proliferation and were exposed to a large dose of roentgen rays to eliminate polymorphonuclear leucocytes. Stains for mucous and for mitochondria were done also. The evidence obtained favors the theory that the viral infection does not destroy any of the cells of the bronchial epithelium. Inclusion bodies were found in the cytoplasm, making it seem likely instead that viral particles grow in colony-like aggregations and that liberation of virus into the lumen takes place not only by simple extrusion of inclusions but also by detachment of inclusion-laden globular portions of the cytoplasm.

Increase in epithelial mast cell numbers in the nasal mucosa of patients with perennial allergic rhinitis

1996 ◽  
Vol 110 (10) ◽  
pp. 929-933 ◽  
Author(s):  
A. Slater ◽  
L. A. Smallman ◽  
A. B. Drake-Lee
Keyword(s):  
Allergic Rhinitis ◽  
Mast Cells ◽  
Light Microscopy ◽  
Nasal Mucosa ◽  
Inferior Turbinate ◽  
Control Group ◽  
Perennial Allergic Rhinitis ◽  
Immunohistochemical Technique ◽  
Cell Numbers ◽  
Staining Methods

AbstractThe aim of the study was to compare the numbers and distribution of mast cells in the nasal mucosa of perennial allergic rhinitis (PAR) patients and controls, as demonstrated by different staining methods for light microscopy.Biopsies of inferior turbinate mucosa were taken from 10 patients with PAR and 10 patients undergoing septoplasty or septorhinoplasty (control group). Sections for light microscopy were stained with azure A, chloroacetate esterase and an ABC immunohistochemical technique using antibody to tryptase.Three times more mast cells were found in the epithelium of PAR patients compared to controls using the immunohistochemical technique (p = 0.0074). This method demonstrated considerably more mast cells than the other stains.The increase in epithelial mast cells is consistent with the migration of mast cells seen in seasonal allergic rhinitis, and this may be important in the phenomenon of nasal priming seen after repeated antigen exposure.

scholarly journals Immunogold-silver staining: new method of immunostaining with enhanced sensitivity.

1983 ◽  
Vol 31 (7) ◽  
pp. 938-944 ◽  
Author(s):  
C S Holgate ◽  
P Jackson ◽  
P N Cowen ◽  
C C Bird
Keyword(s):  
Silver Staining ◽  
New Method ◽  
Precipitation Reaction ◽  
Human Tonsil ◽  
Fixed Tissue ◽  
Immunogold Staining ◽  
Enhanced Sensitivity ◽  
Secondary Antiserum ◽  
Silver Staining Method ◽  
Staining Methods

A new method for demonstrating antigens in paraffin sections of formol sublimate-fixed tissue is described that utilizes an "indirect" immunohistological technique employing immunoglobulin adsorbed to colloidal gold as the secondary antiserum. The gold particles introduced to antigenic sites are revealed by a silver precipitation reaction. This technique, the immunogold-silver staining method, is of much enhanced sensitivity (up to 200-fold) as compared with standard immunoperoxidase and immunogold staining methods. The results have been confirmed in a study of immunoglobulins in reactive human tonsil. The use of this new method for double immunolabeling is also described.

scholarly journals Simultaneous localization of proteoglycan by light and electron microscopy using toluidine blue O. A study of epiphyseal cartilage.

1976 ◽  
Vol 24 (5) ◽  
pp. 621-629 ◽  
Author(s):  
N Shepard ◽  
N Mitchell
Keyword(s):  
Electron Microscopy ◽  
Light Microscopy ◽  
Toluidine Blue ◽  
Light And Electron Microscopy ◽  
Uranyl Acetate ◽  
Collagen Fibrils ◽  
Epiphyseal Cartilage ◽  
Fixation Method ◽  
Fixed Tissue

The simultaneous localization of proteoglycan by light and electron microscopy was demonstrated by fixing epiphyseal cartilage in a glutaraldehyde toluidine blue O solution. Sections cut for light microscopy viewing and those cut for electron microscopy required no further staining, although, in the latter case, staining with uranyl acetate and lead improved the overall contrast. By this technique, electron-dense structures were seen concentrated about the cells which were actively synthesizing matrix, and these structures appeared to bind collagen fibrils. Similar structures were not seen in conventionally fixed tissue. They could also not be identified when the specimens were previously incubated with the proteoglycan-digesting enzyme, papain, prior to toluidine blue O fixation. The toluidine blue O fixation method, unlike conventional fixation and staining, retained proteoglycan in the pericellular areas of actively synthesizing cells and made it visible by light and electron microscopy. It appears that proteoglycans is both precipitated and stained by the presence of toluidine blue O during fixation.

scholarly journals Sperm morphological and morphometric evaluation in captive collared peccaries (Pecari tajacu)

2013 ◽  
Vol 33 (7) ◽  
pp. 924-930 ◽  
Author(s):  
Patrícia C. Sousa ◽  
Erika A.A. Santos ◽  
Ana L.P. Souza ◽  
Gabriela L. Lima ◽  
Felipe F.P.C. Barros ◽  
...  
Keyword(s):  
Light Microscopy ◽  
Sperm Morphology ◽  
Blue Staining ◽  
Image Analyzer ◽  
Pecari Tajacu ◽  
Staining Methods ◽  
Collared Peccary ◽  
Bengal Rose ◽  
Phenol Blue ◽  
Morphometric Evaluation

The aim of this study was to compare different staining methods for the evaluation of sperm morphology by light microscopy and also to describe the morphometry of the entire sperm in collared peccaries (Pecari tajacu). Semen from 10 males was obtained by electroejaculation and evaluated for sperm motility, vigor, and concentration. Semen smears were prepared through three different staining methods: Bengal rose, brome-phenol blue, and eosin-nigrosin. Smears were evaluated under light microscopy and sperm morphologic alterations were determined in percentage. In addition, sperm morphometric analysis was conducted by light microscopy coupled to image analyzer software. The smears stained with Bengal Rose provide the best results for the visualization of the sperm tail, midpiece, and head. The use of eosin-nigrosin stain did not allow an adequate impregnation, and some sperm presented a few contrasts with the background. A higher incidence of bent coiled tails was verified in the use of brome-phenol blue staining (P<0.05). Through morphometric evaluation, it was observed that the tail occupies the greatest proportion (89%) of the sperm which presents a discretely elongated head. According to the results, the use of the Bengal Rose stain is recommended for the morphologic evaluation of the collared peccary sperm.

scholarly journals DISTRIBUTION OF PEROXISOMES (MICROBODIES) IN THE NEPHRON OF THE RAT

1969 ◽  
Vol 42 (2) ◽  
pp. 501-518 ◽  
Author(s):  
Margaret E. Beard ◽  
Alex B. Novikoff
Keyword(s):  
Light Microscopy ◽  
Electron Micrographs ◽  
Oxidase Activity ◽  
Hydroxy Acid ◽  
Acid Oxidase ◽  
Outer Medulla ◽  
Fixed Tissue ◽  
Low Levels ◽  
Proximal Convolution ◽  
Pars Convoluta

The distribution of peroxisomes (microbodies) in the rat nephron was studied cytochemically, using glutaraldehyde- or formaldehyde-fixed tissue, by means of α-hydroxy acid oxidase activity in light microscopy or oxidation of 3,3'-diaminobenzidine (DAB) at pH 9 in both light and electron microscopy.The two cytochemical methods show peroxisomes to be nearly sperical particles found only in cells of the proximal convoluted tubule. Lysosomes were identified in the same or parallel sections, with ß-glycerophosphate or 5'-cytidylic acid as substrate. They are found in all cells of the nephron. These cytochemical methods visualize the two organelles for light microscopy; they also permit unequivocal differentiation of all kidney peroxisomes from lysosomes in electron micrographs. Peroxisomes are larger and more reactive in the cells of the pars descendens (P3 segment) of the proximal convolution, located in the outer medulla and medullary rays, than in the cells of the pars convoluta (P1 and P2 segments), situated in the cortex. In contrast, lysosomes are much smaller in the P3 segment and larger and more reactive in the P1 and P2 segments. In all cells of the proximal convolution, peroxisomes tend to be concentrated nearer the base of the cells than do lysosomes. Mitochondria in P3 cells also show low levels of DAB oxidation at pH 6, in contrast to those in P1 and P2 cells. The possibility is discussed that P3 cells possess an extramitochondrial means of oxidation in which peroxisome oxidases play an important role.

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