The Redox Dysregulation Visualized by Electron Microscopic Localization of Cerium Perhydroxide in The Kidney of (Nzb X Nzw)F1 Mice.

1997 ◽  
Vol 3 (S2) ◽  
pp. 59-60
Author(s):  
Megumi Nishikawa ◽  
Li-Li Chen ◽  
Rie Igarashi ◽  
Tomoko Nakazawa ◽  
Eizo Aikawa
Keyword(s):  
Electron Microscopy ◽  
Renal Involvement ◽  
Radical Reactions ◽  
Oxygen Species ◽  
Rt Pcr ◽  
Standard Medium ◽  
Electron Microscopic ◽  
Ultrathin Sections ◽  
Pcr Method ◽  
Electron Microscopic Localization

Redox dysregulation is known as pathogenesis of renal involvement in autoimmune lupus mice [1,2]. However, the behavior of reactive oxygen species (ROS) is still unclear [3]. The purpose of this study is to make clear the localization of hydrogen perhydroxide which is generated as intermediate substances of radical reactions.Modified Brigg's method [4] was used to determine the generation of ROS in renal tissues of (NZB x NZW)F1 mice. The renal specimens were incubated with a standard medium, which consisted of 0. IM tris-malate buffer (pH7.4) with 7% sucrose, lmM CeCl3 and lOmM aminotriazole at 37° C for 30 min and ultrathin sections were prepared and examined by electron microscopy. Lecithinized-recombinant-superoxide dismutase (SOD) was used as redox regulator, which is ROS inhibitor. SOD (1000 U/kg) was injected 3 times a week, tail-intravenously to Fl mice from 4 to 60 weeks of age. The mRNA of inflammatory cytokines and IkB was detected by RT-PCR method.

Use of Uranium-Labeled Antibodies for Electron Microscopic Localization of Various Antigens in Mammalian Cells

1967 ◽  
Vol 25 ◽  
pp. 136-137
Author(s):  
J. P. Petrali ◽  
E. J. Donati ◽  
L. A. Sternberger
Keyword(s):  
Endoplasmic Reticulum ◽  
Hela Cells ◽  
Mammalian Cells ◽  
Specific Antiserum ◽  
Electron Microscopic ◽  
E Coli ◽  
Cytoplasmic Membranes ◽  
Viral Progeny ◽  
Ultrathin Sections ◽  
Electron Microscopic Localization

Specific contrast is conferred to subcellular antigen by applying purified antibodies, exhaustively labeled with uranium under immunospecific protection, to ultrathin sections. Use of Seligman’s principle of bridging osmium to metal via thiocarbohydrazide (TCH) intensifies specific contrast. Ultrathin sections of osmium-fixed materials were stained on the grid by application of 1) thiosemicarbazide (TSC), 2) unlabeled specific antiserum, 3) uranium-labeled anti-antibody and 4) TCH followed by reosmication. Antigens to be localized consisted of vaccinia antigen in infected HeLa cells, lysozyme in monocytes of patients with monocytic or monomyelocytic leukemia, and fibrinogen in the platelets of these leukemic patients. Control sections were stained with non-specific antiserum (E. coli).In the vaccinia-HeLa system, antigen was localized from 1 to 3 hours following infection, and was confined to degrading virus, the inner walls of numerous organelles, and other structures in cytoplasmic foci. Surrounding architecture and cellular mitochondria were unstained. 8 to 14 hours after infection, antigen was localized on the outer walls of the viral progeny, on cytoplasmic membranes, and free in the cytoplasm. Staining of endoplasmic reticulum was intense and focal early, and weak and diffuse late in infection.

scholarly journals ELECTRON MICROSCOPIC LOCALIZATION OF THE NEPHROTOXIC ANTIBODY IN THE GLOMERULI OF THE RAT AFTER INTRAVENOUS APPLICATION OF PURIFIED NEPHRITOGENIC ANTIBODY-FERRITIN CONJUGATES

1968 ◽  
Vol 127 (5) ◽  
pp. 867-878 ◽  
Author(s):  
Arnold Vogt ◽  
Hermann Bockhorn ◽  
Keniti Kozima ◽  
Masamichi Sasaki
Keyword(s):  
Electron Microscopy ◽  
Basement Membrane ◽  
Intravenous Injection ◽  
Fluorescent Antibody ◽  
Fluorescent Antibody Technique ◽  
Electron Microscopic ◽  
Antibody Technique ◽  
Disappearance Time ◽  
Filtration Surface ◽  
Electron Microscopic Localization

Nephritis in rats was induced by intravenous injection of purified ferritin-conjugated rabbit and duck nephrotoxic globulin. Using the fluorescent antibody technique, the same capillary pattern was found as that in glomeruli of rats receiving uncoupled nephrotoxic globulin. Electron microscopy revealed a heavy accumulation of the basement membrane-fixed antibody almost exclusively at the endothelial side. A higher concentration of ferritin was demonstrable in the peripheral basement membrane. The once-fixed antibody remained at the site of reaction though decreasing with time. The half-disappearance time seemed to be shorter than that of the uncoupled nephrotoxic globulin. No difference in localization was observed between rabbit and duck antibody. At least 40 basement membrane-fixed antibody molecules from the rabbit per 3000 mµ2 of filtration surface were needed to cause immediate nephritis. To induce nephritis using duck antibody, a greater number of basement membrane-fixed antibody seemed to be necessary. No evidence of specific reaction with constituents of glomerular cells was obtained.

scholarly journals Inorganic trimetaphosphatase as a histochemical marker for lysosomes in light and electron microscopy.

1977 ◽  
Vol 25 (12) ◽  
pp. 1381-1384 ◽  
Author(s):  
S B Doty ◽  
C E Smith ◽  
A R Hand ◽  
C Oliver
Keyword(s):  
Electron Microscopy ◽  
Incubation Medium ◽  
Soft Tissues ◽  
Light And Electron Microscopy ◽  
Electron Microscopic ◽  
Cytochemical Method ◽  
Microscopic Localization ◽  
Histochemical Marker ◽  
Electron Microscopic Localization

A new cytochemical method is presented for the light and electron microscopic localization of lysosomes in mineralized and soft tissues. Inorganic trimetaphosphate is used as substrate in a lead chelate incubation medium at pH 3.9. Lysosomes in several tissues are strongly reactive, and reaction product is frequently present in Golgi saccules and GERL. The reaction can be differentiated from acid glycerophosphatase activity, is relatively insensitive to fixation and demineralization procedures, and the reaction is often complete after short incubation times.

Electron microscopic analysis of objects in light microscopic sections

1978 ◽  
Vol 36 (2) ◽  
pp. 80-81
Author(s):  
Arvid B. Maunsbach
Keyword(s):  
Electron Microscopy ◽  
Light And Electron Microscopy ◽  
Microscopic Analysis ◽  
Electron Microscopic Level ◽  
Semithin Section ◽  
Cover Glass ◽  
Electron Microscopic ◽  
Thin Sections ◽  
Glass Slides ◽  
Ultrathin Sections

Structural studies in experimental biology or in pathology are frequently extended from the light to the electron microscopic level. This is often done by cutting both semithin (about 1 μm) and thin sections from the same tissue block after embedding for electron microscopy. However, in many studies it would be of great value to analyse the same structure both by light and electron microscopy, i.e. to be able to study by electron microscopy an object which is first detected by light microscopy in a semithin section. To achieve this, a method has been developed by which ultrathin sections are cut directly from the semithin section containing the object of interest.Semithin sections, about 1 μ in thickness, are cut from Epon or Vestopal embedded tissue. The sections are placed on ordinary glass slides and stained with toluidine blue. The sections are studied in the light microscope without a cover glass or mounted in water.

Organs of a Baikal seal affected by a morbillivirus

1990 ◽  
Vol 48 (3) ◽  
pp. 968-969
Author(s):  
O.I. Belykh ◽  
Ye.V. Likhoshway ◽  
Yu.V. Solodun ◽  
O.A. Goldberg ◽  
V.P. Kumarev
Keyword(s):  
Electron Microscopy ◽  
Measles Virus ◽  
Present Report ◽  
Protein A ◽  
Uranyl Acetate ◽  
Kidney Cells ◽  
Electron Microscopic ◽  
Microscopic Studies ◽  
Liver And Kidney ◽  
Ultrathin Sections

The population of Baikal seals Phoca sibirica has been plagued in 1987-88 by an unknown disease. Oligonucleotide probing of nucleic acids isolated from tissues of ill and dead animals, as well as immunological evidence and clinical data suggested that seals were infected by a morbillivirus. Morbillivirus antigen has been vizualized in dead seal tissues by immunoelectron microscopy (preembedding technique).The present report gives outline of electron microscopic studies of the tissues of infected Baikal seals. Morbillivirus antigens were vizualized as clusters of gold spheres by postembedding technique with monoclonal antibodies against measles virus and protein A-colloid gold conjugates in nuclei and cytoplasm of liver and kidney cells. Some clusters were associated with virus-like particles having a diameter of 80-100 nm. Electron microscopy of ultrathin sections stained with uranyl acetate revealed nucleocapsides having length of up to 1400 nm, and a diameter of 13-17 nm, morphologically similar to measles and seals distemper virus.

scholarly journals Photoconversion and electron microscopic localization of the fluorescent axon tracer fluoro-ruby (rhodamine-dextran-amine).

1993 ◽  
Vol 41 (5) ◽  
pp. 777-782 ◽  
Author(s):  
L C Schmued ◽  
L F Snavely
Keyword(s):  
Electron Microscopy ◽  
Green Light ◽  
Microscopic Level ◽  
Synaptic Connectivity ◽  
Electron Microscopic Level ◽  
Light Microscopic Level ◽  
Electron Microscopic ◽  
Axon Transport ◽  
Conventional Electron Microscopy ◽  
Electron Microscopic Localization

Fluoro-Ruby, the fluorescent tetramethylrhodamine-dextran-amine used to demonstrate anterograde axon transport, has been successfully photoconverted and subsequently localized by electron microscopy. The photoconversion was accomplished by irradiating the tissue with green light while bathing it in a solution containing DAB. The tissue could then be examined by brightfield microscopy or processed for conventional electron microscopy. Potential advantages of the technique include greater permanence and contrast at the light microscopic level and the ability to resolve synaptic connectivity at the electron microscopic level.

scholarly journals Electron Microscopic Localization of Russell Bodies in the Human Plasma Cell

Blood ◽  
1960 ◽  
Vol 16 (3) ◽  
pp. 1307-1312 ◽  
Author(s):  
RONALD A. WELSH
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Human Plasma ◽  
Plasma Cell ◽  
Plasma Cells ◽  
Electron Microscopic ◽  
Degenerative Process ◽  
Microscopic Localization ◽  
Electron Microscopic Localization

Abstract The location of Russell bodies in the human plasma cell was shown by electron microscopy to be within the intracisternal space of the endoplasmic reticulum. The significance of this finding was discussed from the standpoint of possible intracellular function of the endoplasmic reticulum. The appearance of the affected plasma cells tended to negate a degenerative process, and the suggestion was offered that the Russell body results from a condensation of intracisternal secretion.

Electron microscopic study of respiratory syncitial (RS) virus: A new approach

1988 ◽  
Vol 46 ◽  
pp. 312-313
Author(s):  
R. Alain ◽  
F. Bélanger ◽  
L. Berthiaume ◽  
M. Trudel
Keyword(s):  
Electron Microscopy ◽  
Microscopic Study ◽  
Electron Microscopic Study ◽  
Post Infection ◽  
Final Concentration ◽  
Negative Staining ◽  
Electron Microscopic ◽  
New Approach ◽  
Ok Cells ◽  
Ultrathin Sections

The respiratory syncytial (RS) virus is included in the Paramyxoviridae family, genus Pneumovirus. In the present study, three strains were compared: human (HRS), bovine (BRS) and caprine (CRS). The ultrastructure of HRS virus has been previously described according to conventional methods of negative staining and ultrathin sectioning (Berthiaume et al., 1974). Recently, bridges between BRS particles in ultrathin sections were noted (Bélanger et al., 1988). Bridges were also observed with CRS (unpublished results). We were interested in studying, by negative staining, what produced these bridges and why they were present on BRS and CRS particles and not on HRS.Human, bovine and caprine RS virus were propagated in ovine kidney (OK) cells. After 5 to 7 days post-infection, when viral titers were at a maximum, supernatants were collected. They were separated in two parts: the first aliquot was used without treatment immediately after collection; the second aliquot was immediately fixed with glutaraldehyde (final concentration of 0,5% (v/v)) and after ten minutes fixation, samples were prepared for electron microscopy.

Electron Microscopic Study of the Polymyxin Treated Goat Erythrocytes

1984 ◽  
Vol 39 (7-8) ◽  
pp. 776-780
Author(s):  
T. K. Mandal ◽  
S. N. Chatterjee
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Spherical Shape ◽  
Polymyxin B ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
Ultrathin Sections ◽  
Treatment Changes ◽  
Dose Dependent ◽  
Scanning Electron

Abstract Polymyxin B produced dose dependent changes in the surface topography of the goat erythrocyte cells. Transformation from the normal biconcave discs through crenated structures to the final rounded or spherical shape was recorded by scanning electron microscopy. A maxim um of three to four crenations per cell was recorded corresponding to a polymyxin dose of 15.62 ng/ml. Transmission electron microscopy of the ultrathin sections of treated or untreated erythrocytes indicated that the crenations were formed by protrusions of the plasma membrane, occurring presumably because of the local increase of membrane fluidity after polymyxin treatment. Changes in the shape of the erythrocytes to the ultimate rounded forms were also indicated by the transmission electron microscopy.

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