Quantitative Electron Holography Of Magnetic Materials

1999 ◽  
Vol 5 (S2) ◽  
pp. 32-33
Author(s):  
M. R. McCartney
Keyword(s):  
Spatial Resolution ◽  
Nanometer Scale ◽  
Objective Lens ◽  
Electron Holography ◽  
Field Versus ◽  
Wide Range ◽  
Phase Images ◽  
Phase Resolution ◽  
Range Of Values

Off-axis electron holography is a powerful method for providing quantitative micromagnetic structure to nanometer-scale resolution. One of the main challenges in determining magnetic induction from reconstructed phase images is the separation of phase shifts due to electrostatic and thickness effects from magnetostatic effects. We have used in-situ magnetization reversal to remove electrostatic and thickness effects which would otherwise prevent quantification of the induction. The Philips CM200-FEG microscope which was used for the holography described here is equipped with a powerful mini-lens below the specimen enabling 2nm spatial resolution (∼5nm phase resolution) and only a small residual field at the sample. In addition, the objective lens field versus current has been calibrated so that external vertical fields over a wide range of values may be applied to the sample. Tilting the sample in the presence of the vertical field has the effect of ‘applying an inplane component. This combination of high spatial resolution, quantitative analysis and in situ capabilities allows for the study of individual defects and provides experimental input for comparison with simulations.

Electron Holography of Electromagnetic Fields

1997 ◽  
Vol 3 (S2) ◽  
pp. 1059-1060
Author(s):  
J.E. Bonevich
Keyword(s):  
Magnetic Properties ◽  
Software Package ◽  
Phase Changes ◽  
Nanometer Scale ◽  
Objective Lens ◽  
Electron Holography ◽  
Wide Range ◽  
Electric And Magnetic Fields ◽  
Flame Burner ◽  
Conventional Microscopy

Electron holography can lend crucial insights to understanding the subtle manifestations of electro-magnetism in a wide range of materials. Whereas conventional microscopy is sensitive only to the intensity, holography reveals the phase changes in coherent electron wavefronts making it a unique tool to probe electric and magnetic fields on the nanometer scale. We have employed electron holography to characterize materials for mean inner potential measurements and also their electric and magnetic properties.Electron holograms were acquired in a 300 kV FE-TEM under two optical conditions: the standard high resolution mode was employed for mean inner potential measurements; to examine the intrinsic electromagnetic states, a lower resolution mode was used whereby the objective lens is turned off and the diffraction lens images the specimen. Digitally acquired holograms were reconstructed with the HolograFREE software package.Nanophase TiO2 particles generated in a flame burner system were found to have unusual central features. The rutile particles appear to contain faceted voids, raising the question whether the feature is truly a void or a secondary amorphous phase.

scholarly journals Urban Distributed Acoustic Sensing Using In-Situ Fibre Beneath Bern, Switzerland

2020 ◽  
Author(s):  
Krystyna Smolinski ◽  
Patrick Paitz ◽  
Daniel Bowden ◽  
Pascal Edme ◽  
Felix Kugler ◽  
...  
Keyword(s):  
Spatial Resolution ◽  
Seismic Waves ◽  
Velocity Structure ◽  
Road Traffic ◽  
Hazard Analysis ◽  
Noise Sources ◽  
Acoustic Sensing ◽  
Wide Range ◽  
Distributed Acoustic Sensing

<p>Anticipating the risks natural hazards pose to an urban environment requires an understanding of the shallow Earth structure of the region. While urban infrastructure often hinders the deployment of a traditional seismic array, Distributed Acoustic Sensing (DAS) technology facilitates the use of existing telecommunication fibre-optic cables for seismic observation, with spatial resolution down to the metre scale.</p><p>Through collaboration with the SWITCH foundation, we were able to use existing, in-situ fibres beneath Bern, Switzerland for seismic data acquisition over two weeks, covering a distance of 6 km with a spatial resolution of 2 m. This allowed for not only real-time visualisation of anthropogenic noise sources (e.g. road traffic), but also of the propagation of resulting seismic waves.</p><p>Data is analysed in the time and frequency domain to explore the range of signals captured and to assess the consistency of data quality along the cable. The local velocity structure can be constrained using both noise correlations and deterministic signals excited by traffic.</p><p>Initial results reveal the ability of DAS to capture signals over a wide range of frequencies and distances, and show promise for utilising urban DAS data to perform urban seismic tomography and hazard analysis.</p>

scholarly journals High-resolution inventory to capture glacier disintegration in the Austrian Silvretta

2021 ◽  
Author(s):  
Andrea Fischer ◽  
Bernd Seiser ◽  
Kay Helfricht ◽  
Martin Stocker-Waldhuber
Keyword(s):  
High Resolution ◽  
Spatial Resolution ◽  
Ice Age ◽  
Surface Elevation ◽  
Glacier Changes ◽  
Wide Range ◽  
Elevation Data ◽  
Glacier Ice ◽  
Geodetic Mass Balance

Abstract. Eastern Alpine glaciers have been receding since the LIA maximum, but the majority of glacier margins could be delineated unambiguously for the last Austrian glacier inventories. Even debris-covered termini, changes in slope, colour or the position of englacial streams enabled at least an in situ survey of glacier outlines. Today the outlines of totally debris-covered glacier ice are fuzzy and raise the theoretical discussion if these glaciogenic features are still glaciers and should be part of the respective inventory – or part of an inventory of transient cryogenic landforms. A new high-resolution glacier inventory (area and surface elevation) was compiled for the years 2017 and 2018 to quantify glacier changes for the Austrian Silvretta region in full. Glacier outlines were mapped manually, based on orthophotos and elevation models and patterns of volume change of 1 to 0.5 m spatial resolution. The vertical accuracy of the DEMs generated from 6 to 8 LiDAR points per m2 is in the order of centimetres. calculated in relation to the previous inventories dating from 2004/2006 (LiDAR), 2002, 1969 (photogrammetry) and to the Little Ice Age maximum extent (moraines). Between 2004/06 and 2017/2018, the 46 glaciers of the Austrian Silvretta lost −29 ± 4 % of their area and now cover 13.1 ± 0.4 km2. This is only 32 ± 2 % of their LIA extent of 40.9 ± 4.1 km2. The area change rate increased from −0.6 %/year (1969–2002) to −2.4 %/year (2004/06–2017/18). The annual geodetic mass balance showed a loss increasing from −0.2 ± 0.1 m w.e./year (1969–2002) to –0.8 m ±0.1 w.e./year (2004/06–2017/18) with an interim peak in 2002–2004/06 at −1.5 ± 0.7 m w.e./year. Identifying the glacier outlines offers a wide range of possible interpretations of former glaciers that have evolved into small and now totally debris-covered cryogenic geomorphological structures. Only the patterns and amounts of volume changes allow us to estimate the area of the buried glacier remnants. To keep track of the buried ice and its fate, and to distinguish increasing debris cover from ice loss, we recommend inventory repeat frequencies of three to five years and surface elevation data with a spatial resolution of one metre.

Off-Axis Electron Holography of Self-Assembled Co Nanoparticle Rings

2007 ◽  
Vol 1026 ◽  
Author(s):  
Takeshi Kasama ◽  
Rafal E. Dunin-Borkowski ◽  
Michael R. Scheinfein ◽  
Steven L. Tripp ◽  
Jie Liu ◽  
...  
Keyword(s):  
Room Temperature ◽  
Phase Image ◽  
Objective Lens ◽  
Electron Holography ◽  
Zero Field ◽  
Transmission Electron ◽  
Out Of Plane ◽  
Self Assembled ◽  
Magnetic States

AbstractWe use off-axis electron holography in the transmission electron microscope (TEM) to study magnetic flux closure (FC) states in self-assembled nanoparticle rings that each contain between five and eleven 25-nm-diameter Co crystals. Electron holograms are acquired at room temperature in zero-field conditions after applying chosen magnetic fields to the samples in situ in the TEM by partially exciting the conventional microscope objective lens. Mean inner potential contributions to the phase shift are determined by turning the samples over, and subsequently subtracted from each recorded phase image to obtain magnetic induction maps. Our results show that most nanoparticle rings form FC remanent magnetic states, and occasionally onion-like states. Although the chiralities (the directions of magnetization) of the FC states are determined by the shapes, sizes and positions of the constituent nanoparticles, reproducible magnetization reversal of each ring can be achieved by using an out-of-plane magnetic field of between 1600 and 2500 Oe.

Dark field electron holography for quantitative strain measurements with nanometer-scale spatial resolution

2009 ◽  
Vol 95 (5) ◽  
pp. 053501 ◽  
Author(s):  
David Cooper ◽  
Jean-Paul Barnes ◽  
Jean-Michel Hartmann ◽  
Armand Béché ◽  
Jean-Luc Rouviere
Keyword(s):  
Spatial Resolution ◽  
Dark Field ◽  
Nanometer Scale ◽  
Electron Holography ◽  
Strain Measurements ◽  
Field Electron ◽  
Dark Field Electron

X-ray microanalysis of airway surface liquid in the mouse

2005 ◽  
Vol 288 (5) ◽  
pp. L874-L878 ◽  
Author(s):  
Inna Kozlova ◽  
Harriet Nilsson ◽  
Mia Phillipson ◽  
Brigitte Riederer ◽  
Ursula Seidler ◽  
...  
Keyword(s):  
Elemental Composition ◽  
Ionic Composition ◽  
Airway Surface Liquid ◽  
X Ray ◽  
Relative Paucity ◽  
Wide Range ◽  
Surface Liquid ◽  
Bead Method ◽  
Range Of Values

The ionic composition of airway surface liquid (ASL) has been debated, and, in particular for the mouse, a wide range of values has been published. Two techniques were developed to measure the elemental composition of the ASL. X-ray microanalysis of ASL was carried out at low temperature on trachea removed from isoflurane-anesthetized animals and shock-frozen. In the second technique, dextran beads were placed on top of the epithelium of the trachea removed from pentobarbital-anesthetized animals, left to equilibrate with the ASL, dried, and subjected to X-ray microanalysis. Both techniques showed that mouse tracheal ASL has significantly lower concentrations of Na and Cl (∼60–80 mM) than serum. Differences between the two techniques were due to different sampling of mucus. CFTR(−/−) mice had significantly higher concentrations of Na and Cl in their ASL than age-matched controls. Pilocarpine or isoproterenol stimulation significantly reduced the ion concentrations in tracheal ASL. ASL was also collected with the dextran bead method from the nasal cavity in situ in pentobarbital-anesthetized animals. In control animals, the elemental composition of nasal fluid was similar to that of tracheal ASL. Pilocarpine stimulation caused a significant increase in Na, Cl, and K; stimulation with isoproterenol or phenylephrine caused a significant increase only in K. It is concluded that mouse ASL under unstimulated conditions is hypotonic, which may be related to the relative paucity of submucosal glands in the mouse trachea.

scholarly journals Fiducial Reference Measurements for Vegetation Bio-Geophysical Variables: An End-to-End Uncertainty Evaluation Framework

2021 ◽  
Vol 13 (16) ◽  
pp. 3194
Author(s):  
Luke A. Brown ◽  
Fernando Camacho ◽  
Vicente García-Santos ◽  
Niall Origo ◽  
Beatriz Fuster ◽  
...  
Keyword(s):  
Spatial Resolution ◽  
Reference Data ◽  
High Spatial Resolution ◽  
Evaluation Framework ◽  
Uncertainty Evaluation ◽  
Uncertainty Estimates ◽  
Wide Range ◽  
End To End ◽  
Reference Measurements

With a wide range of satellite-derived vegetation bio-geophysical products now available to users, validation efforts are required to assess their accuracy and fitness for purpose. Substantial progress in the validation of such products has been made over the last two decades, but quantification of the uncertainties associated with in situ reference measurements is rarely performed, and the incorporation of uncertainties within upscaling procedures is cursory at best. Since current validation practices assume that reference data represent the truth, our ability to reliably demonstrate compliance with product uncertainty requirements through conformity testing is limited. The Fiducial Reference Measurements for Vegetation (FRM4VEG) project, initiated by the European Space Agency, is aiming to address this challenge by applying metrological principles to vegetation and surface reflectance product validation. Following FRM principles, and in accordance with the International Standards Organisation’s (ISO) Guide to the Expression of Uncertainty in Measurement (GUM), for the first time, we describe an end-to-end uncertainty evaluation framework for reference data of two key vegetation bio-geophysical variables: the fraction of absorbed photosynthetically active radiation (FAPAR) and canopy chlorophyll content (CCC). The process involves quantifying the uncertainties associated with individual in situ reference measurements and incorporating these uncertainties within the upscaling procedure (as well as those associated with the high-spatial-resolution imagery used for upscaling). The framework was demonstrated in two field campaigns covering agricultural crops (Las Tiesas–Barrax, Spain) and deciduous broadleaf forest (Wytham Woods, UK). Providing high-spatial-resolution reference maps with per-pixel uncertainty estimates, the framework is applicable to a range of other bio-geophysical variables including leaf area index (LAI), the fraction of vegetation cover (FCOVER), and canopy water content (CWC). The proposed procedures will facilitate conformity testing of moderate spatial resolution vegetation bio-geophysical products in future validation exercises.

scholarly journals A Versatile Linker for Probes Targeting Hydrolases via In Situ labeling

2021 ◽  
Author(s):  
Jun Liu ◽  
Zixin Chen ◽  
Chao Cui ◽  
Ashton L Sigler ◽  
Lina Cui
Keyword(s):  
Spatial Resolution ◽  
Molecular Mechanisms ◽  
Test Tube ◽  
Quinone Methide ◽  
Covalent Bonds ◽  
Fluorescent Reporter ◽  
Wide Range ◽  
Key Factor

Hydrolases are important molecules that are involved in a wide range of biological functions and their activities are tightly regulated in healthy or diseased states. Detecting or imaging the activities of hydrolases, therefore, can reveal underlying molecular mechanisms in the context of cells to organisms, and their correlation with different physiological conditions can therefore be used in diagnosis. Due to the nature of hydrolases, substrate-based probes can be activated in their catalytic cycles, and cleavage of covalent bonds frees reporter moieties. For test-tube type bulk detection, spatial resolution is not a measure of importance, but for cell- or organism-based detection or imaging, spatial resolution is a key factor for probe sensitivity that influences signal-to-background ratio. One strategy to improve spatial resolution of the probes is to form a covalent linkage between the reporter moiety and intracellular proteins upon probe activation by the enzyme. In this work, we developed a generalizable linker chemistry that would allow in situ labeling of various imaging moieties via quinone methide species. To do so, we synthesized probes containing a monofluoromethyl or a difluoromethyl groups for β-galactosidase activation, while using fluorescein as a fluorescent reporter. The labeling efficacy of these two probes was evaluated in vitro. The probe bearing a monofluormethyl group exhibited superior labeling efficiency in imaging β-galactosidase activity in living cells. This study provides a versatile linker for applying quinone methide chemistry in the development of hydrolase-targeting probes involving in situ labeling.

scholarly journals FEI Titan G2 60-300 HOLO

2016 ◽  
Vol 2 ◽  
Author(s):  
Chris Boothroyd ◽  
András Kovács ◽  
Karsten Tillmann
Keyword(s):  
Electron Gun ◽  
Fourth Generation ◽  
Objective Lens ◽  
Electron Holography ◽  
Pole Piece ◽  
High Brightness ◽  
Transmission Electron ◽  
Schottky Type ◽  
Technical Specifications

The FEI Titan G2 60-300 HOLO is a unique fourth generation transmission electron microscope, which has been specifically designed for the investigation of electromagnetic fields of materials using off-axis electron holography. It has a Lorentz lens to allow magnetic field free imaging plus two electron biprisms, which in combination enable more uniform holographic fringes to be used. The instrument also has an ultra-wide objective lens pole piece gap which is ideal for <em>in situ</em> experiments. For these purposes, the FEI Titan G2 60-300 HOLO is equipped with a Schottky type high-brightness electron gun (FEI X-FEG), an image C<sub>s</sub> corrector (CEOS), a post-column energy filter system (Gatan Tridiem 865 ER) as well as a 4 megapixel CCD system (Gatan UltraScan 1000 XP). Typical examples of use and technical specifications for the instrument are given below.

Comparison of Deterministic and Linear Cosine Spatial Filtering of Transmission Electron Micrographs

1972 ◽  
Vol 30 ◽  
pp. 596-597
Author(s):  
David A. Ansley
Keyword(s):  
Spherical Aberration ◽  
Focal Length ◽  
Chromatic Aberration ◽  
Spatial Filter ◽  
Operating Conditions ◽  
Objective Lens ◽  
List Type ◽  
Spatial Filters ◽  
Transmission Electron ◽  
Wide Range

The coherence of the electron flux of a transmission electron microscope (TEM) limits the direct application of deconvolution techniques which have been used successfully on unmanned spacecraft programs. The theory assumes noncoherent illumination. Deconvolution of a TEM micrograph will, therefore, in general produce spurious detail rather than improved resolution.A primary goal of our research is to study the performance of several types of linear spatial filters as a function of specimen contrast, phase, and coherence. We have, therefore, developed a one-dimensional analysis and plotting program to simulate a wide 'range of operating conditions of the TEM, including adjustment of the:(1) Specimen amplitude, phase, and separation(2) Illumination wavelength, half-angle, and tilt(3) Objective lens focal length and aperture width(4) Spherical aberration, defocus, and chromatic aberration focus shift(5) Detector gamma, additive, and multiplicative noise constants(6) Type of spatial filter: linear cosine, linear sine, or deterministic

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