Imaging and Volumetric Quantitation of Vascular Corrosion Casts with Laser Scanning Confocal Microscopy.

2000 ◽  
Vol 6 (S2) ◽  
pp. 562-563 ◽  
Author(s):  
K. Czymmek ◽  
R. C. Wagner ◽  
F. E. Hossler ◽  
R. Kao
Keyword(s):  
Confocal Microscopy ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Quantitative Information ◽  
Laser Scanning Confocal Microscopy ◽  
Hot Water ◽  
Corrosion Casts ◽  
Sem Images ◽  
Corrosion Cast ◽  
Vascular Corrosion Casts

Vascular corrosion casts provide faithful replicas of the three-dimensional anatomy of blood vessel system of organs and tissues. In addition, corrosion casts can be used to obtain quantitative information regarding vessel and tissue spaces. However, morphometric measurements of SEM images of corrosion casts is difficult due to severe specimen tilt, parallax and the inability to image 3D casts from all angles. We present evidence that confocal microscopy can be used to collect all of the 3D information of a corrosion cast in a z-series of optical slices. Surface and volumetric parameters of casted and non-casted space are readily retrievable from a stack of optical sections through a corrosion cast.Various tissues and organs were exsanguinated with heparenized saline following cannulization of regional arteries and casts were made by infusing Mercox resin or Mercox diluted with 20% methyl methacrylate monomer. Tissues were macerated with alternating rinses in 5% KOH and hot water.

Laser Scanning Confocal Microscopy and Three-Dimesnsional Reconstruction of the Mitotic Spindles of Unequally Dividing Embryonic Cells

1990 ◽  
Vol 48 (3) ◽  
pp. 166-167
Author(s):  
J. Holy ◽  
G. Schatten
Keyword(s):  
Confocal Microscopy ◽  
Mitotic Spindle ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Laser Scanning Confocal Microscopy ◽  
Two Dimensions ◽  
Embryonic Cells ◽  
Mitotic Spindles ◽  
Cleavage Division ◽  
Scanning Confocal Microscopy

One of the classic limitations of light microscopy has been the fact that three dimensional biological events could only be visualized in two dimensions. Recently, this shortcoming has been overcome by combining the technologies of laser scanning confocal microscopy (LSCM) and computer processing of microscopical data by volume rendering methods. We have employed these techniques to examine morphogenetic events characterizing early development of sea urchin embryos. Specifically, the fourth cleavage division was examined because it is at this point that the first morphological signs of cell differentiation appear, manifested in the production of macromeres and micromeres by unequally dividing vegetal blastomeres.The mitotic spindle within vegetal blastomeres undergoing unequal cleavage are highly polarized and develop specialized, flattened asters toward the micromere pole. In order to reconstruct the three-dimensional features of these spindles, both isolated spindles and intact, extracted embryos were fluorescently labeled with antibodies directed against either centrosomes or tubulin.

Three-Dimensional Laser-Scanning Confocal Microscopy of In Situ Hybridization in the Skin

1994 ◽  
Vol 16 (1) ◽  
pp. 44-51 ◽  
Author(s):  
Stephen E. Mahoney ◽  
Stephen W. Paddock ◽  
Louis C. Smith ◽  
Dorothy E. Lewis ◽  
Madeleine Duvic
Keyword(s):  
In Situ Hybridization ◽  
Confocal Microscopy ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Laser Scanning Confocal Microscopy ◽  
Scanning Confocal Microscopy

scholarly journals Three-Dimensional Nano-Morphology of Carbon Nanotube/Epoxy Filled Poly(methyl methacrylate) Microcapsules

Materials ◽  
2019 ◽  
Vol 12 (9) ◽  
pp. 1387 ◽  
Author(s):  
M. Galip Icduygu ◽  
Meltem Asilturk ◽  
M. Akif Yalcinkaya ◽  
Youssef K. Hamidi ◽  
M. Cengiz Altan
Keyword(s):  
Confocal Microscopy ◽  
Methyl Methacrylate ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Laser Scanning Confocal Microscopy ◽  
Poly Methyl Methacrylate ◽  
Scanning Confocal Microscopy ◽  
Electron Microscopy Analysis ◽  
Microscopy Analysis ◽  
Average Size

The three-dimensional nano-morphology of poly(methyl methacrylate; PMMA) microcapsules filled with carbon nanotubes (CNTs) and epoxy resin were investigated by various microscopy methods, including a novel, laser scanning confocal microscopy (LSCM) method. Initially, PMMA microcapsules containing various amounts of CNTs were synthesized by a solvent evaporation method. Scanning electron microscopy analysis showed that pore-free, smooth-surface microcapsules formed with various types of core-shell morphologies. The average size of CNT/epoxy/PMMA microcapsules was shown to decrease from ~52 μm to ~15 μm when mixing speed during synthesis increased from 300 rpm to 1000 rpm. In general, the presence of CNTs resulted in slightly larger microcapsules and higher variations in size. Moreover, three-dimensional scans obtained from confocal microscopy revealed that higher CNT content increased the occurrence and size of CNT aggregates inside the microcapsules. Entrapped submicron air bubbles were also observed inside most microcapsules, particularly within those with higher CNT content.

Some possibilities of representing microcirculation in human spleen

Biologia ◽  
2009 ◽  
Vol 64 (6) ◽  
Author(s):  
Paulína Gálfiová ◽  
Ivan Varga ◽  
Martin Kopáni ◽  
Peter Michalka ◽  
Jana Michalková ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Laser Scanning Confocal Microscopy ◽  
The Other ◽  
Corrosion Casts ◽  
Histochemical Analysis ◽  
Human Spleen ◽  
Scanning Confocal Microscopy ◽  
Scanning Electron

AbstractThe representation of microcirculation can be approached in several ways. One of the possibilities is to represent the endothelium (endothelial or sinus lining cells) and their basement membrane on the basis of detecting the known components and the expression of the surface antigenes by the methods of immuno-, enzyme- or lectino-histochemical analysis, or by staining or impregnation histological methods. The other possibility is the examination of samples by transmission and scanning electron microscopy. For three-dimensional demonstration corrosion casts techniques or laser scanning confocal microscopy can be used. In this paper we describe the survey of immuno-, enzyme- and lectino-histochemical characteristics of selected components of microcirculation and our own results of its demonstration in human spleen.

scholarly journals Paired arbuscules in the Arum-type arbuscular mycorrhizal symbiosis with Linum usitatissimum

2003 ◽  
Vol 81 (5) ◽  
pp. 457-463 ◽  
Author(s):  
Sandy Dickson ◽  
Peter Schweiger ◽  
F Andrew Smith ◽  
Bengt Söderström ◽  
Sally Smith
Keyword(s):  
Confocal Microscopy ◽  
Surface Area ◽  
Linum Usitatissimum ◽  
Laser Scanning ◽  
Time Course ◽  
Developmental Stages ◽  
Three Dimensional ◽  
Arbuscular Mycorrhizal ◽  
Laser Scanning Confocal Microscopy ◽  
Mycorrhizal Symbiosis

Experiments were conducted to investigate the "paired" arbuscules characteristic of Arum-type mycorrhizal colonization in Linum usitatissimum L. The development and senescence of arbuscular structures were followed in a time course study. Roots were freeze-sectioned longitudinally and mycorrhizal structures visualized using nitroblue tetrazolium, a vital stain to indicate metabolically active arbuscules and intercellular hyphae, followed by acid fuchsin counterstaining. Arbuscules were imaged using laser scanning confocal microscopy. The volume and surface area of each arbuscule of a developing paired structure were measured using three-dimensional imaging software. Arbuscules occurred in pairs in adjacent cortical cells arising from a single, radial intercellular hypha. These "paired" arbuscules often appeared to be at different developmental stages. Logistic regression and measurement of surface area indicated that there was a delay in initiation of the second arbuscule.Key words: Arum-type arbuscular mycorrhiza, double staining, metabolic activity, morphology, confocal microscopy, Linum usitatissimum.

Characterizing the Influence of Drying on Ink Absorption Using Reconstructed Images by Laser Scanning Confocal Microscopy

2014 ◽  
Vol 904 ◽  
pp. 59-62 ◽  
Author(s):  
Jian Guo Li ◽  
Ying Li
Keyword(s):  
Confocal Microscopy ◽  
Penetration Depth ◽  
Laser Scanning ◽  
Three Dimensional ◽  
Laser Scanning Confocal Microscopy ◽  
Coated Paper ◽  
Drying Temperature ◽  
Scanning Confocal Microscopy ◽  
Distribution Uniformity ◽  
The Relationship

The objective of this experiment was to investigate the relationship between drying and ink absorption using laser scanning confocal microscopy (LSCM). Fluorescent ink was used to observe and characterize ink penetration and distribution by LSCM. Three-dimensional images of ink penetration were obtained by reconstructing all XY plane images. Reconstructed images were used to describe ink absorption in coated paper by LSCM. The results implied that it was reliable and effective using LSCM to characterize the ink penetration depth and distribution uniformity. This method could not damage the specimen and did not need fluorescent dye to stain the specimen, which decreased the errors by hand operation. The results indicated that drying temperature affected ink penetration depth and distribution evenness. Higher and lower drying temperature could not contribute to ink absorption uniformity. With the drying temperature increasing, ink penetration depth in coated paper increased.

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