Imaging Resin-Cast Osteocyte Lacuno-Canalicular System at Bone-Bioactive Glass Interface by Scanning Electron Microscopy

2010 ◽  
Vol 16 (2) ◽  
pp. 132-136 ◽  
Author(s):  
Alejandro A. Gorustovich
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Bioactive Glass ◽  
Microscopy Technique ◽  
Resin Cast ◽  
Calcium Phosphorus ◽  
Rat Tibia ◽  
Glass Interface ◽  
Pass Through ◽  
Scanning Electron

AbstractThe morphology of the osteocyte lacuno-canalicular system at the bone-biomaterial implant-interface has not been fully investigated. In this study, the resin-cast scanning electron microscopy technique was used, for the first time, to image the lacuno-canalicular network within neoformed bone around bioactive glass (BG) particles implanted in rat tibia bone marrow. The most salient finding was that the osteocyte canaliculi pass through the calcium-phosphorus layer formed at the bone-BG interface and reach the silica-rich layer of the reacted BG.

scholarly journals Perbedaan efektifitas penutupan tubulus dentin antara pasta gigi yang mengandung bioaktif glass (novamin) dan strontium chloride

2019 ◽  
Vol 8 (2) ◽  
pp. 96
Author(s):  
Profilia Shinta ◽  
Ketut Suardita ◽  
Moch. Mudjiono
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Bioactive Glass ◽  
Strontium Chloride ◽  
Dentinal Tubule ◽  
Two Samples ◽  
Dentinal Hypersensitivity ◽  
Tubule Occlusion ◽  
Scanning Electron ◽  
At Home

Background : Dentinal hypersensitivity (DH) is a painful clinical condition and is characterized by pain arising from exposed dentin in response to various stimuli. Various treatment modalities are available to treat dentinal hypersensitivity which include at-home and in-office treatment. At home treatment generally consists of a variety of dentrifices containing different constituents like strontium chloride and bioactive glass (Novamin). These agents cause occlusion of dentinal tubules thereby reducing hypersensitivity.Purpose: This study  was  to evaluate the effects of different desensitizing dentifrices on dentinal tubule occlusion by scanning electron microscopy (SEM) of strontium chloride and bioactive glass (Novamin) pastes in the treatment of dentinehypersensitivity (DH) Methods: sixteen extractedspecimens from bovine incisors teeth with randomized into 2 groups (n=8). The crowns were removed from the root and the crown  were sectioned longitudinally into two parts (in a mesiodistaldirection). The cervical toothwere resulting in two samples per tooth.Dentinal tubules were exposedand thesamples provided a 4 mm x 4 mm area of exposed dentinal tubules.Group 1 (treated with strontium chloride), Group 2 (treated with  bioactive glass (novamin))After each treatment for seven days, tubule occlusion on dentin were analyzed by scanning electron microscopy  (SEM).The data were analyzed using “mann-whitneytest” (p<0.05).Result: Groups bioactive glass (novamin) showed tubule occlusion highest when compared with groups strontium chloride.Conclusion: bioactive glass (novamin) paste showed tubule occlusion highest with strontium chloride

Environmental Scanning Electron Microscopy Technique to Identify Asbestos Phases Inside Ferruginous Bodies

2013 ◽  
Vol 19 (2) ◽  
pp. 420-424 ◽  
Author(s):  
Alessandro Croce ◽  
Maya Musa ◽  
Mario Allegrina ◽  
Paolo Trivero ◽  
Caterina Rinaudo
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Chemical Elements ◽  
Environmental Scanning Electron Microscopy ◽  
Environmental Scanning ◽  
Thin Sections ◽  
The Core ◽  
Microscopy Technique ◽  
Ferruginous Bodies ◽  
Scanning Electron

AbstractFerruginous bodies observed in lungs of patients affected by mesothelioma, asbestosis, and pulmonary carcinoma are important to relate the illness to exposure, environmental or occupational, to asbestos. Identification of the inorganic phase constituting the core of the ferruginous bodies, formed around asbestos but also around phases different from asbestos, is essential for legal purposes. Environmental scanning electron microscopy/energy dispersive spectroscopy was used to identify the fibrous mineral phase in the core of ferruginous bodies observed directly in thin sections of tissue, without digestion of the biological matrix. Spectra were taken with sequential analyses along a line crossing the core of the ferruginous bodies. By comparing the spectra taken near to and far from the core, the chemical elements that make up the core could be identified.

Latex Spheres as Immunologic Markers to Demonstrate the Binding of Human Salivary Immunoglobulins to Streptococcus mutans

1976 ◽  
Vol 55 (5) ◽  
pp. 879-885 ◽  
Author(s):  
G.R. Riviere ◽  
W.R. Cotton ◽  
J.L. Derkowski
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Streptococcus Mutans ◽  
Bacterial Cell ◽  
Oral Bacteria ◽  
Scanning Electron Microscopy Technique ◽  
Microscopy Technique ◽  
Immunologic Markers ◽  
Latex Beads ◽  
Scanning Electron

A scanning electron microscopy technique is described that uses latex beads as immunologic markers for detecting the interaction of salivary antibody with Streptococcus mutans. A Labeling Index (beads per bacterial cell) demonstrated that human salivary antibodies bound at a significantly greater degree to S mutans than to non oral bacteria.

scholarly journals Linked Redox Precipitation of Sulfur and Selenium under Anaerobic Conditions by Sulfate-Reducing Bacterial Biofilms

2003 ◽  
Vol 69 (12) ◽  
pp. 7063-7072 ◽  
Author(s):  
Simon L. Hockin ◽  
Geoffrey M. Gadd
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Elemental Sulfur ◽  
Environmental Scanning Electron Microscopy ◽  
Environmental Scanning ◽  
Elemental Selenium ◽  
Sulfate Reducing ◽  
Pass Through ◽  
Biofilm Matrix ◽  
Scanning Electron

ABSTRACT A biofilm-forming strain of sulfate-reducing bacteria (SRB), isolated from a naturally occurring mixed biofilm and identified by 16S rDNA analysis as a strain of Desulfomicrobium norvegicum, rapidly removed 200 μM selenite from solution during growth on lactate and sulfate. Elemental selenium and elemental sulfur were precipitated outside SRB cells. Precipitation occurred by an abiotic reaction with bacterially generated sulfide. This appears to be a generalized ability among SRB, arising from dissimilatory sulfide biogenesis, and can take place under low redox conditions and in the dark. The reaction represents a new means for the deposition of elemental sulfur by SRB under such conditions. A combination of transmission electron microscopy, environmental scanning electron microscopy, and cryostage field emission scanning electron microscopy were used to reveal the hydrated nature of SRB biofilms and to investigate the location of deposited sulfur-selenium in relation to biofilm elements. When pregrown SRB biofilms were exposed to a selenite-containing medium, nanometer-sized selenium-sulfur granules were precipitated within the biofilm matrix. Selenite was therefore shown to pass through the biofilm matrix before reacting with bacterially generated sulfide. This constitutes an efficient method for the removal of toxic concentrations of selenite from solution. Implications for environmental cycling and the fate of sulfur and selenium are discussed, and a general model for the potential action of SRB in selenium transformations is presented.

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