Diversity of seed storage protein patterns of Slovak accessions in jointed goatgrass (Aegilops cylindrica Host.)

2011 ◽  
Vol 9 (2) ◽  
pp. 222-223
Author(s):  
Edita Gregová ◽  
Pavol Hauptvogel ◽  
René Hauptvogel ◽  
Gábor Vörösváry ◽  
Gábor Málnási Csizmadia

Variations in seed storage protein patterns were investigated for six accessions of jointed goatgrass (Aegilops cylindrica) populations collected from Slovakia within the framework of the bilateral Co-operation in Science and Technology between the Slovak Republic and Hungary. The study covered populations collected from the southwestern (localities: Sered and Dunajská Streda), southern (localities: Chlaba and Kamenica nad Hronom) and southeastern (localities: Cierna nad Tisou and Dobra) parts of Slovakia. Analysis of profiles of seed storage proteins – glutenins and gliadins – was carried out using acid polyacrylamide gel electrophoresis and sodium dodecyl sulphate polyacrylamide gel electrophoresis. All accessions have a uniform three-band high molecular weight glutenin pattern with CxCyDy subunit composition. The highest variations in gliadin bands among the populations were observed from Cierna nad Tisou. There were small differences among the populations from Chlaba and Dobra. The lowest variations were in populations from Sered, Dunajska Streda and Kamenica nad Hronom. The present investigation showed that these jointed goatgrass populations are valuable genetic resources for wheat crop improvement programmes.

2011 ◽  
Vol 9 (2) ◽  
pp. 256-259
Author(s):  
Zuzana Šramková ◽  
Edita Gregová ◽  
Svetlana Šliková ◽  
Ernest Šturdík

The objective of our study was to determine the composition of high-molecular weight-glutenin subunits (HMW-GS) in 120 cultivars of common wheat (Triticum aestivum L.). Fourteen alleles and 34 allelic compositions were detected using sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The most frequent HMW-GS alleles at the Glu-A1, Glu-B1 and Glu-D1 loci were null (57.1%), 7+9 (43.3%) and 5+10 (61.9%), respectively. However, low-frequency HMW-GS alleles were also observed, such as 13+16, 20, 21, 7 and 18, encoded by the Glu-B1 locus, and 4+12, encoded by the Glu-D1 locus. The wheat–rye 1BL.1RS translocation was identified in 25 cultivars, using acid polyacrylamide gel electrophoresis. The Glu-score varied greatly, and some lines reached the maximum value of 10.


1983 ◽  
Vol 25 (4) ◽  
pp. 266-273 ◽  
Author(s):  
Eva Klozová ◽  
Jiřina Švachulová ◽  
J. Smartt ◽  
E. Hadač ◽  
Věra Turková ◽  
...  

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