fusarium fungi
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2022 ◽  
Vol 12 (1) ◽  
pp. 473
Author(s):  
Daniela Gwiazdowska ◽  
Katarzyna Marchwińska ◽  
Krzysztof Juś ◽  
Pascaline Aimee Uwineza ◽  
Romuald Gwiazdowski ◽  
...  

The presence of Fusarium fungi and their toxic metabolites in agricultural crops contributes to significant quantitative and qualitative losses of crops, causing a direct threat to human and animal health and life. Modern strategies for reducing the level of fungi and mycotoxins in the food chain tend to rely on natural methods, including plant substances. Essential oils (EOs), due to their complex chemical composition, show high biological activity, including fungistatic properties, which means that they exhibit high potential as a biological plant protection factor. The aim of this study was to determine the fungistatic activity of three EOs against F. graminearum, and the reduction of mycotoxin biosynthesis in corn and wheat grain. All tested EOs effectively suppressed the growth of F. graminearum in concentrations of 5% and 10%. Cinnamon and verbena EOs also effectively reduced the ergosterol (ERG) content in both grains at the concentration of 1%, while at the 0.1% EO concentration, the reduction in the ERG amount depended on the EO type as well as on the grain. The degree of zearalenone (ZEA) reduction was consistent with the inhibition of ERG biosynthesis, while the reduction in deoxynivalenol (DON) was not consistent with this parameter.


2022 ◽  
Vol 25 (8) ◽  
pp. 874-881
Author(s):  
N. S. Zhemchuzhina ◽  
M. I. Kiseleva ◽  
T. M. Kolomiets ◽  
I. B. Ablova ◽  
A. P. Glinushkin ◽  
...  

In order to prevent crop yield losses from the most dangerous and economically important pathogenic organisms, it is necessary not only to monitor the virulence gene pool, but also to study the nature of pathogen variability and determine the potential for the emergence of new genes and races. This requires centralized collections of fungal cultures characterized by a set of stable strains to provide for phytopathological, immunological, breeding, genetic, toxicological, parasitological and other studies. The State Collection of Phytopathogenic Microorganisms of the ARSRIP is the State Depository of Phytopathogenic microorganisms that are non-pathogenic to humans or farmed animals. Currently, it has more than 4,500 accessions of plant pathogenic strains of fungi, oomycetes, bacteria, viruses, phytoplasmas, and the collection is updated annually. For this purpose, the study of the inter- and intraspecific genetic diversity of genus Fusarium was carried out in agricultural systems of the Krasnodar Territory. In 2020, the State Collection of Phytopathogenic Microorganisms was supplemented with 13 strains of Fusarium fungi isolated from tissues of winter wheat plants collected in several locations of the Krasnodar region. The complex of Fusarium fungi revealed on winter wheat usually included Fusarium oxysporum, F. culmorum, F. lolii, F. graminearum, F. fujikuroi, F. sporotrichioides, etc. The effect of the preceding crop on the frequency of Fusarium species isolated from winter wheat was observed. After series cloning of collected isolates, 21 strains of different fungal species characterized by stable morphology traits and known pathogenic and phytotoxic properties were selected for collection replenishment. Significant differences in pathogenic activity were revealed between fungi belonging to either the same or different species; the manifestation of this activity varied from the absence of any effect of spore suspensions on seedling development to a complete inhibition of their growth. The phytotoxic activity towards wheat seedlings varied from medium to high. Species possessing a high intensity of phytotoxic activities are the most dangerous for wheat, since they promote accumulation of dangerous phytotoxins in plant tissues.


Toxins ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 884
Author(s):  
Maciej Żelechowski ◽  
Tomasz Molcan ◽  
Katarzyna Bilska ◽  
Kamil Myszczyński ◽  
Jacek Olszewski ◽  
...  

Soybean is an important, high protein source of food and feed. However, like other agricultural grains, soybean may pose a risk to human and animal health due to contamination of the grains with toxigenic Fusaria and associated mycotoxins. In this study, we investigated the diversity of Fusaria on a panel of 104 field isolates obtained from soybean grains during the growing seasons in 2017–2020. The results of species-specific PCR analyses showed that Fusarium avenaceum was the most common (n = 40) species associated with soybean grains in Poland, followed by F. equiseti (n = 22) and F. sporotrichioides (11 isolates). A set of isolates, which was not determined based on PCR analyses, was whole genome sequenced. Multiple sequence analyses using tef-1α, top1, rpb1, rpb2, tub2, pgk, cam and lsu genes showed that most of them belonged to Equiseti clade. Three cryptic species from this clade: F. clavum, F. flagelliforme and FIESC 31 (lacking Latin binomial) were found on soybean for the first time. This is the first report demonstrating the prevalence of Fusaria on soybean grains in Poland.


2021 ◽  
Vol 25 (7) ◽  
pp. 732-739
Author(s):  
O. P. Gavrilova ◽  
T. Yu. Gagkaeva ◽  
A. S. Orina ◽  
A. S. Markova ◽  
A. D. Kabashov ◽  
...  

Fusarium disease of oats reduces yield quality due to decreasing germination that is caused by then contamination of grain with mycotoxins produced by Fusarium fungi. The aim of this study was to characterize the resistance of naked breeding lines of oats to fungal grain infection and to contamination with T-2 and HT-2 toxins. Thirteen naked oat breeding lines and two naked varieties, Nemchinovsky 61 and Vyatskiy, as well as a husked variety Yakov, were grown under natural conditions in the Nemchinovka Federal Research Center in 2019–2020. The contamination of grain with fungi was determined by the mycological method and real-time PCR. The analysis of mycotoxins was carried out by ELISA. In oats, Alternaria (the grain infection was 15–90 %), Cochliobolus (1–33 %), Cladosporium (1–19 %), Epicoccum (0–11 %), and Fusarium (3–17 %) fungi prevailed in the grain mycobiota. The predominant Fusarium species were F. poae (its proportion among Fusarium fungi was 49–68 %) and F. langsethiae (29–28 %). The highest amounts of F. langsethiae DNA ((27.9–71.9)×10–4 pg/ng) and T-2/HT-2 toxins (790–1230 μg/kg) were found in the grain of husked oat Yakov. Among the analysed naked oat lines, the amount of F. langsethiae DNA varied in the range of (1.2–42.7)×10–4 pg/ng, and the content of T-2/HT-2 toxins was in the range of 5–229 μg/kg. Two oat breeding lines, 54h2476 and 66h2618, as well as a new variety, Azil (57h2396), can be characterized as highly resistant to infection with Fusarium fungi and contamination with mycotoxins compared to the control variety Vyatskiy. 


2021 ◽  
Vol 845 (1) ◽  
pp. 012069
Author(s):  
A A Vypritskaya ◽  
A A Kuznetsov ◽  
V V Chekmarev ◽  
G N Buchneva ◽  
N N Dubrovskaya ◽  
...  

Abstract The study of prevalence and species composition of Fusarium fungi on sunflower and winter wheat crops was carried out in Tambov region. In 1992-2020, 15 Fusarium fungi were identified on sunflower crops. It was identified that the predominant species infecting sunflower plants and seeds are Fusarium oxysporum, F. oxysporum var. orthoceras, F. verticillioides, and F. tricinctum. The prevalence of these pathogens was 16.3-21.8%. On the grain of winter wheat, Fusarium poae, F. equiseti and F. sporotrichioides were more common (19.6-28.6%). It was found that the seeds of Lgovskaya 8, Don Awnless and Don’s Governor were less affected by fusarium infection (by 3 - 4%). It was observed that Fusarium fungi are associated with certain winter wheat varieties. Research results may be useful for specialists studying diseases of sunflower and wheat.


2021 ◽  
Vol 55 (5) ◽  
pp. 433-446
Author(s):  
A. V. Karelov ◽  
O. I. Borzykh ◽  
N. O. Kozub ◽  
I. O. Sozinov ◽  
L. A. Yanse ◽  
...  
Keyword(s):  

2021 ◽  
pp. 103875
Author(s):  
Marek Pernica ◽  
Blanka Kyralová ◽  
Zdeněk Svoboda ◽  
Rastislav Boško ◽  
Iveta Brožková ◽  
...  

2021 ◽  
Vol 104 (2) ◽  
pp. 124-127
Author(s):  
N. A. Krupenko* ◽  
S. F. Buga ◽  
A. G. Zhukovskiy ◽  
I. N. Odintsova ◽  
A. A. Zhukovskaya ◽  
...  

Fusarium fungi are the main causal agents of root rot of winter cereals in Belarus. As many as 12 different species were identified, with occurrence being dependent on the cereal host species and weather conditions during the growing season. Lack of precipitation from April to July led to decreasing fungal biodiversity. Fusarium pathogen complex on wheat roots was formed by F. culmorum, F. avenaceum, F. equiseti, F. oxysporum, whereas F. equiseti and F. oxysporum prevailed on triticale and rye, and F. solani, F. avenaceum and F. equiseti dominated on barley root systems. The infestation of root with F. oxysporum increased under dry conditions. In contrast, F. culmorum was isolated from root system of wheat and triticale more frequently when rainfall was sufficient. For the first time, F. cerealis and F. tricinctum were isolated from triticale, and F. cerealis from rye only, in the Republic of Belarus.


2021 ◽  
Author(s):  
Jing Zhang ◽  
Huabo Chen ◽  
Xiaomei Wan ◽  
Mingming Jiang ◽  
Yichao Ding ◽  
...  

Abstract Purpose:To analyze the etiology, characteristics, and treatment outcomes of paediatric fungal keratitis in northern China.Methods:The medical records of children (< 18 years old) diagnosed with fungal keratitis at the Shandong Eye Institute from 1996 to 2017 were reviewed for demographic features, risk factors, seasonal variation, clinical characteristics, laboratory findings, and treatment strategies.Results:Forty-five children (46 eyes) were included. Fungal keratitis in children accounted for 16.4% of all of the children with infectious keratitis, which was lower than that in adults (59.4%, p < 0.001). Unexplained fungal keratitis accounted for 26.1%. Plant trauma (23.9%) and wind and sand into the eyes (21.7%) ranked second and third, respectively. Fusarium fungi infection was found in 69.9%, and 31.0% were infected by Aspergillus fungi. The corneal ulcer area was positively correlated with a peripheral blood neutrophil/lymphocyte ratio (NLR) at the first visit (r = 0.310, p = 0.036). Voriconazole had the highest drug sensitivity rate. A total of 67.4% (31 eyes) underwent therapeutic keratoplasty (PKP 21 eyes; LKP 10 eyes). The recurrence rate was 3.2% (due to an Aspergillus infection). The best corrected visual acuity (BCVA) after treatment in children ≤ 8 years (median 20/60) was lower than the BCVA in children > 8 years (median 20/50, p = 0.035).Conclusions:Fungal keratitis are less common in children than in adults. The main infection was due to Fusarium fungi. The preferred antifungal drug was voriconazole. LKP treatment is preferred for Fusarium infection keratitis.


2021 ◽  
Vol 344 (1) ◽  
pp. 92-97
Author(s):  
Yu. S. Lebedin ◽  
A. S. Orina ◽  
O. P. Gavrilova ◽  
T. Y. Gagkaeva ◽  
V. N. Maigurova ◽  
...  

Relevance. The scientific community and agricultural producers are looking for a solution to the problem of how to protect consumers from the effects of hazardous mycotoxins in agricultural products. One of the tools, which will be considered in this article, is a screening system that allows determining the quan-titative indicators of wheat grain infection by toxin-producing fungi in a short time.Methods. The method of quantitative PCR with the detection of fungal DNA and the method of enzyme immunoassay with the detection of Fusarium antigens were used to characterize the grain infection.Results. We established the lower critical limit of DNA content which is 3955·10-4 pg/ng and the lower critical limit of Fusarium antigens which is 596 U/g based on the analyzes of grain fungi contamination in model experiments. Grain should be subjected to mycotoxins analysis upon detection of these critical lim-its. All batches of grain can be used in production without additional analyzes if their values determined by analytical methods are below that critical points. The obtained values can be quantitative benchmarks corresponding to the standard visual analysis of grain contamination currently described in GOST 31646-2012


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