Incubated Protein Reduction and Digestion on an Electrowetting-on-Dielectric Digital Microfluidic Chip for MALDI-MS

Due to its simple structure, low consumption of energy but strong driving forces, Electrowetting on Dielectric (EWOD) is used most frequently in digital microfluidics for manipulation and control of droplets. In this paper, the internal mechanism of EWOD is explained though establishing the geometric model of the unipolar board structure digital microfluidic chip. And the boundary conditions of equations are determined. Three coupling physical fields: electric field, flow field and temperature field in the digital microfluidic chip are simulated and analyzed. With the electric field equation coupled, Navier-Stokes equations and energy equation of the temperature control, the numerical simulation of the chip is conducted. The results show that the internal flow of micro-droplets is counterclockwise and swirling flow. The external flow velocity of micro-droplet is greater than the internal velocity. In addition, micro-droplets near the electrode applied temperature are higher than the internal temperature. Surface micromachining technologies are employed to fabricate the chip. Experimental results show that the droplet can be driven in a velocity of 25cm/s. It will possibly provide an effective solution to the manipulation of droplets.

Download Full-text

Electrochemical detection on electrowetting-on-dielectric digital microfluidic chip

Talanta ◽

10.1016/j.talanta.2011.03.073 ◽

2011 ◽

Vol 84 (5) ◽

pp. 1384-1389 ◽

Cited By ~ 37

Author(s):

Chanpen Karuwan ◽

Kreeta Sukthang ◽

Anurat Wisitsoraat ◽

Ditsayut Phokharatkul ◽

Viyapol Patthanasettakul ◽

...

Keyword(s):

Electrochemical Detection ◽

Microfluidic Chip ◽

Electrowetting On Dielectric ◽

Digital Microfluidic

Download Full-text

Efficient Radiosynthesis of 3'-Deoxy-3'-18F-Fluorothymidine Using Electrowetting-on-Dielectric Digital Microfluidic Chip

Journal of Nuclear Medicine ◽

10.2967/jnumed.113.121053 ◽

2013 ◽

Vol 55 (2) ◽

pp. 321-328 ◽

Cited By ~ 26

Author(s):

M. R. Javed ◽

S. Chen ◽

H.-K. Kim ◽

L. Wei ◽

J. Czernin ◽

...

Keyword(s):

Microfluidic Chip ◽

Electrowetting On Dielectric ◽

Digital Microfluidic

Download Full-text

EWOD-Based Digital Microfluidic Chip and its Application

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.157-158.1071 ◽

2012 ◽

Vol 157-158 ◽

pp. 1071-1074 ◽

Cited By ~ 2

Author(s):

Yin Xia Chang ◽

Si Xiang Zhang ◽

Jian Yi Li

Keyword(s):

Microfluidic Chip ◽

Printed Circuit Board ◽

New Technologies ◽

New Technology ◽

Circuit Board ◽

Commercial Application ◽

Good Prospect ◽

Electrowetting On Dielectric ◽

Major Application ◽

Digital Microfluidic

As a new technology, digital microfluidic chip based on EWOD (Electrowetting on Dielectric) has good prospect of application in biological, medical, chemical and optic fields. Firstly, the working principle is introduced. Then the major application domains are summarized. Finally, the key problems in design and two fabrication methods are discussed, the clean-room-processing chip is more precise and PCB(Printed Circuit Board) chip is cheaper. New technologies developed in these fields speed up the step from lab to commercial application.

Download Full-text

Drop driving on digital microfluidic chip

Optics and Precision Engineering ◽

10.37188/ope.20202811.2488 ◽

2020 ◽

Vol 28 (11) ◽

pp. 2488-2496

Author(s):

Hong WANG ◽

◽

Jie ZHENG ◽

Yan-peng YAN ◽

Song WANG ◽

...

Keyword(s):

Microfluidic Chip ◽

Digital Microfluidic

Download Full-text

Electrowetting-on-dielectric (EWOD) digital microfluidic device for in-line workup in organic reactions: A critical step in the drug discovery work cycle

Sensors and Actuators B Chemical ◽

10.1016/j.snb.2020.129252 ◽

2021 ◽

Vol 330 ◽

pp. 129252

Author(s):

Matin Torabinia ◽

Udaya Sree Dakarapu ◽

Parham Asgari ◽

Junha Jeon ◽

Hyejin Moon

Keyword(s):

Drug Discovery ◽

Microfluidic Device ◽

Organic Reactions ◽

Electrowetting On Dielectric ◽

Critical Step ◽

Work Cycle ◽

Digital Microfluidic

Download Full-text

Ultra high frequency surface acoustic waves enabled Electrode-free digital microfluidic chip

10.1109/ius52206.2021.9593906 ◽

2021 ◽

Author(s):

Shuchang Liu ◽

Weiwei Cui ◽

Wei Pang

Keyword(s):

Microfluidic Chip ◽

Acoustic Waves ◽

High Frequency ◽

Surface Acoustic Waves ◽

Ultra High Frequency ◽

Digital Microfluidic

Download Full-text

Phase separation of multiphase droplets in a digital microfluidic device

Micro and Nano Systems Letters ◽

10.1186/s40486-019-0099-0 ◽

2019 ◽

Vol 7 (1) ◽

Cited By ~ 1

Author(s):

Mun Mun Nahar ◽

Hyejin Moon

Keyword(s):

Phase Separation ◽

Capillary Number ◽

Digital Microfluidics ◽

Separation Performance ◽

Comprehensive Investigation ◽

Electrowetting On Dielectric ◽

Splitting Technique ◽

Droplet Splitting ◽

Two Phases ◽

Digital Microfluidic

Abstract This study reports the first comprehensive investigation of separation of the immiscible phases of multiphase droplets in digital microfluidics (DMF) platform. Electrowetting-on-dielectric (EWOD) actuation has been used to mechanically separate the phases. Phase separation performance in terms of percentage residue of one phase into another phase has been quantified. It was conceived that the residue formation can be controlled by controlling the deformation of the phases. The larger capillary number of the neck forming phase is associated with the larger amount of deformation as well as more residue. In this study, we propose two different ways to control the deformation of the phases. In the first method, we applied different EWOD operation voltages on two phases to maintain equal capillary numbers during phase separation. In the second method, while keeping the applied voltages same on both sides, we tested the phase separation performance by varying the actuation schemes. Less than 2% of residue was achieved by both methods, which is almost 90% improvement compared to the phase separation by the conventional droplet splitting technique in EWOD DMF platform, where the residue percentage can go up to 20%.

Download Full-text