Anionic Polysaccharide-Modified Filter Papers for Rapid Isolation of Extracellular Vesicles from Diverse Samples in a Simple Bind–Wash–Elute Manner

2021 ◽  
Author(s):  
Shanglin Li ◽  
Qiang Liu ◽  
Zhi Geng ◽  
Kaiyi Li ◽  
Tian Zhao ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Rapid Isolation ◽  
Filter Papers

Rapid isolation of extracellular vesicles from diverse biofluid matrices via capillary-channeled polymer fiber solid-phase extraction micropipette tips

The Analyst ◽  
2021 ◽  
Author(s):  
Kaylan K. Jackson ◽  
Rhonda R. Powell ◽  
Terri F. Bruce ◽  
R. Kenneth Marcus
Keyword(s):  
Solid Phase Extraction ◽  
Extracellular Vesicles ◽  
Solid Phase ◽  
Phase Extraction ◽  
Polymer Fiber ◽  
Multiple Methods ◽  
Rapid Isolation ◽  
High Yields

C-CP fiber spin-down tips are employed on a table-top centrifuge to isolate extracellular vesicles from diverse biofluids in less than 15 min. The EVs are isolated in high yields and purity, with the recoveries verified across multiple methods.

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