α-Glucosidase-Triggered Reaction for Fluorometric and Colorimetric Assays Based on the Formation of Silicon-Containing Nanoparticles

Author(s):  
Stanislas Nsanzamahoro ◽  
Wei-Feng Wang ◽  
Ying Zhang ◽  
Cheng-Bo Wang ◽  
Yan-Ping Shi ◽  
...  
Sensors ◽  
2021 ◽  
Vol 21 (8) ◽  
pp. 2797
Author(s):  
Jing-Jhong Gao ◽  
Ching-Wei Chiu ◽  
Kuo-Hsing Wen ◽  
Cheng-Sheng Huang

This paper presents a compact spectral detection system for common fluorescent and colorimetric assays. This system includes a gradient grating period guided-mode resonance (GGP-GMR) filter and charge-coupled device. In its current form, the GGP-GMR filter, which has a size of less than 2.5 mm, can achieve a spectral detection range of 500–700 nm. Through the direct measurement of the fluorescence emission, the proposed system was demonstrated to detect both the peak wavelength and its corresponding intensity. One fluorescent assay (albumin) and two colorimetric assays (albumin and creatinine) were performed to demonstrate the practical application of the proposed system for quantifying common liquid assays. The results of our system exhibited suitable agreement with those of a commercial spectrometer in terms of the assay sensitivity and limit of detection (LOD). With the proposed system, the fluorescent albumin, colorimetric albumin, and colorimetric creatinine assays achieved LODs of 40.99 and 398 and 25.49 mg/L, respectively. For a wide selection of biomolecules in point-of-care applications, the spectral detection range achieved by the GGP-GMR filter can be further extended and the simple and compact optical path configuration can be integrated with a lab-on-a-chip system.


1995 ◽  
Vol 49 (2) ◽  
pp. 156-162 ◽  
Author(s):  
Janet S. MacFall ◽  
Anthony A. Ribeiro ◽  
Gary P. Cofer ◽  
Ko-Hsiu G. Dai ◽  
William Labiosa ◽  
...  

Development of methods for the detection and measurement of aluminum (Al) is crucial for our understanding of Al(III) chemistry and toxicity in natural waters, soil solutions, and environmental samples. Traditional colorimetric assays, by their very nature, alter solution Al(III) chemistry, potentially biasing measurements. Methods based on 27Al NMR spectroscopy have the advantage of being nondestructive and of not altering the chemistry of the solution. Standard commercial NMR probes and sample tubes, unfortunately, are constructed from aluminum-containing components. These materials give substantial background signal, which is detected as a large, broad hump, overwhelming signals from dilute samples. We describe here the construction of two novel NMR probes and a sample container built from a variety of materials with low Al content. The designs feature the use of transversely mounted solenoid coils with aluminum-free sample holders. The sample container features a second chamber which can be filled with an external reference solution. These novel 27Al NMR probes are being used for the NMR spectroscopic investigation and quantitation of natural, dilute (10−6 M) Al(III) samples from the environment.


2018 ◽  
Vol 13 (11) ◽  
pp. 1520-1524 ◽  
Author(s):  
Mary Salve ◽  
Ashwini Wadafale ◽  
Gurushree Dindorkar ◽  
Jayu Kalambe

2016 ◽  
Vol 8 (7) ◽  
pp. 1602-1608 ◽  
Author(s):  
Shintaro Pang ◽  
Lili He

Aptamer–gold nanoparticle (AuNP) based colorimetric assays have become increasingly popular as viable rapid detection methods, but the molecular interactions governing the mechanism and successful interpretation of color changes have not been explored well.


2015 ◽  
Vol 197 (6) ◽  
pp. 815-821 ◽  
Author(s):  
Felipe Padilla-Martínez ◽  
Luz Adriana Carrizosa-Villegas ◽  
Ángeles Rangel-Serrano ◽  
Itzel Paramo-Pérez ◽  
Verónica Mondragón-Jaimes ◽  
...  

2017 ◽  
Vol 105 ◽  
pp. 108-110 ◽  
Author(s):  
Magdalena M. Wiedermann ◽  
Evan S. Kane ◽  
Timothy J. Veverica ◽  
Erik A. Lilleskov

2014 ◽  
Vol 50 (65) ◽  
pp. 9196-9199 ◽  
Author(s):  
Zongzhao Sun ◽  
Ning Zhang ◽  
Yanmei Si ◽  
Shuai Li ◽  
Jiangwei Wen ◽  
...  

Colorimetric mercury(ii) assays with 96-cell plates were achieved by the mercury-stimulated catalytic activity of small silver nanoparticles in switchable gelatin.


2018 ◽  
Vol 1 (7) ◽  
pp. 3741-3746 ◽  
Author(s):  
María Sanromán-Iglesias ◽  
Charles H. Lawrie ◽  
Luis M. Liz-Marzán ◽  
Marek Grzelczak

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