scholarly journals Small-Scale Colorimetric Assays of Intracellular Lactate and Pyruvate in the Nematode Caenorhabditis elegans

10.3791/57807 ◽  
2018 ◽  
Author(s):  
Sumino Yanase ◽  
Kayo Yasuda ◽  
Naoaki Ishii
Keyword(s):  
Caenorhabditis Elegans ◽  
Small Scale ◽  
Lactate And Pyruvate ◽  
Colorimetric Assays

A liquid-culture-based screening approach to study compounds affecting inflammatory processes in Caenorhabditis elegans

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Vu Thu Thuy Nguyen ◽  
Sarah Christina Meyer ◽  
Hanna Rieger ◽  
Kristina Endres
Keyword(s):  
Caenorhabditis Elegans ◽  
Liquid Culture ◽  
Human Cell Line ◽  
Small Scale ◽  
Initial Screening ◽  
Inflammatory Processes ◽  
Biological Sources ◽  
Selection Of ◽  
Screening Approaches

Abstract Discovery of biomedical drugs makes use of novel biological sources of limited availability and is often in need of fast, small-scale initial screening approaches. Here, we present a screening, based on the reporter Caenorhabditis elegans strain IG692, for identification of anti- and pro-inflammatory properties. The elaborated workflow is based on cultivation in fluid and by this, allows fast and reproducible seeding in 96 well plates. LPS and dexamethasone served as reliable controls, comparable to application in the human cell line THP-1. This in vivo approach offers a first step for selection of e.g. natural products or for repurposing of compounds from drug libraries and by this can serve as a tool in drug discovery for inflammatory human diseases.

Reasons to strike first

2019 ◽  
Vol 42 ◽  
Author(s):  
William Buckner ◽  
Luke Glowacki
Keyword(s):  
Intergroup Conflict ◽  
Small Scale

Abstract De Dreu and Gross predict that attackers will have more difficulty winning conflicts than defenders. As their analysis is presumed to capture the dynamics of decentralized conflict, we consider how their framework compares with ethnographic evidence from small-scale societies, as well as chimpanzee patterns of intergroup conflict. In these contexts, attackers have significantly more success in conflict than predicted by De Dreu and Gross's model. We discuss the possible reasons for this disparity.

Exploring Coronal Structures with SOHO

2000 ◽  
Vol 179 ◽  
pp. 403-406
Author(s):  
M. Karovska ◽  
B. Wood ◽  
J. Chen ◽  
J. Cook ◽  
R. Howard
Keyword(s):  
Solar Corona ◽  
Image Enhancement ◽  
Coronal Mass Ejections ◽  
Dynamical Evolution ◽  
Small Scale ◽  
Low Contrast ◽  
Contrast Structures ◽  
Scale Structures ◽  
Small Scale Structures ◽  
Coronal Structures

AbstractWe applied advanced image enhancement techniques to explore in detail the characteristics of the small-scale structures and/or the low contrast structures in several Coronal Mass Ejections (CMEs) observed by SOHO. We highlight here the results from our studies of the morphology and dynamical evolution of CME structures in the solar corona using two instruments on board SOHO: LASCO and EIT.

Scanning tunneling microscopy and atomic force microscopy: New tools for biology

1989 ◽  
Vol 47 ◽  
pp. 778-779
Author(s):  
CE Bracker ◽  
P. K. Hansma
Keyword(s):  
Physical Property ◽  
Scanning Probe ◽  
Scanning Tunneling ◽  
Small Scale ◽  
Tunneling Microscopy ◽  
Force Microscopy ◽  
New Family ◽  
Small Probe ◽  
Atomic Force ◽  
Transmission Electron

A new family of scanning probe microscopes has emerged that is opening new horizons for investigating the fine structure of matter. The earliest and best known of these instruments is the scanning tunneling microscope (STM). First published in 1982, the STM earned the 1986 Nobel Prize in Physics for two of its inventors, G. Binnig and H. Rohrer. They shared the prize with E. Ruska for his work that had led to the development of the transmission electron microscope half a century earlier. It seems appropriate that the award embodied this particular blend of the old and the new because it demonstrated to the world a long overdue respect for the enormous contributions electron microscopy has made to the understanding of matter, and at the same time it signalled the dawn of a new age in microscopy. What we are seeing is a revolution in microscopy and a redefinition of the concept of a microscope.Several kinds of scanning probe microscopes now exist, and the number is increasing. What they share in common is a small probe that is scanned over the surface of a specimen and measures a physical property on a very small scale, at or near the surface. Scanning probes can measure temperature, magnetic fields, tunneling currents, voltage, force, and ion currents, among others.

Superstructures and ordering phenomena in ceramic superconductors

1996 ◽  
Vol 54 ◽  
pp. 710-711
Author(s):  
R. Gronsky
Keyword(s):  
Domain Growth ◽  
Small Displacement ◽  
Small Scale ◽  
Ceramic Superconductors ◽  
Oxygen Sublattices ◽  
Tweed Contrast ◽  
Diffraction Patterns ◽  
Representative Image ◽  
Static Lattice ◽  
Modulation Wavelength

It is now well established that the phase transformation behavior of YBa2Cu3O6+δ is significantly influenced by matrix strain effects, as evidenced by the formation of accommodation twins, the occurrence of diffuse scattering in diffraction patterns, the appearance of tweed contrast in electron micrographs, and the generation of displacive modulation superstructures, all of which have been successfully modeled via simple Monte Carlo simulations. The model is based upon a static lattice formulation with two types of excitations, one of which is a change in oxygen occupancy, and the other a small displacement of both the copper and oxygen sublattices. Results of these simulations show that a displacive superstructure forms very rapidly in a morphology of finely textured domains, followed by domain growth and a more sharply defined modulation wavelength, ultimately evolving into a strong <110> tweed with 5 nm to 7 nm period. What is new about these findings is the revelation that both the small-scale deformation superstructures and coarser tweed morphologies can result from displacive modulations in ordered YBa2Cu3O6+δ and need not be restricted to domain coarsening of the disordered phase. Figures 1 and 2 show a representative image and diffraction pattern for fully-ordered (δ = 1) YBa2Cu3O6+δ associated with a long-period <110> modulation.

The glycomes of Caenorhabditis elegans and other model organisms

2002 ◽  
Vol 69 ◽  
pp. 117-134 ◽  
Author(s):  
Stuart M. Haslam ◽  
David Gems ◽  
Howard R. Morris ◽  
Anne Dell
Keyword(s):  
Caenorhabditis Elegans ◽  
Current Knowledge ◽  
Structural Data ◽  
Model Organisms ◽  
Entire Genome ◽  
C Elegans ◽  
The Face ◽  
Area Of Concern ◽  
Nematode Worm

There is no doubt that the immense amount of information that is being generated by the initial sequencing and secondary interrogation of various genomes will change the face of glycobiological research. However, a major area of concern is that detailed structural knowledge of the ultimate products of genes that are identified as being involved in glycoconjugate biosynthesis is still limited. This is illustrated clearly by the nematode worm Caenorhabditis elegans, which was the first multicellular organism to have its entire genome sequenced. To date, only limited structural data on the glycosylated molecules of this organism have been reported. Our laboratory is addressing this problem by performing detailed MS structural characterization of the N-linked glycans of C. elegans; high-mannose structures dominate, with only minor amounts of complex-type structures. Novel, highly fucosylated truncated structures are also present which are difucosylated on the proximal N-acetylglucosamine of the chitobiose core as well as containing unusual Fucα1–2Gal1–2Man as peripheral structures. The implications of these results in terms of the identification of ligands for genomically predicted lectins and potential glycosyltransferases are discussed in this chapter. Current knowledge on the glycomes of other model organisms such as Dictyostelium discoideum, Saccharomyces cerevisiae and Drosophila melanogaster is also discussed briefly.

Indirect Assessment of Attitudes with Response-Time-Based Measures

2006 ◽  
Vol 37 (3) ◽  
pp. 131-139 ◽  
Author(s):  
Juliane Degner ◽  
Dirk Wentura ◽  
Klaus Rothermund
Keyword(s):  
Social Cognitive ◽  
Incremental Validity ◽  
Self Report ◽  
Small Scale ◽  
Implicit Measures ◽  
Scale Theory ◽  
Starting Point ◽  
Self Reports ◽  
Predicting Behavior ◽  
Social Cognitive Theories

Abstract: We review research on response-latency based (“implicit”) measures of attitudes by examining what hopes and intentions researchers have associated with their usage. We identified the hopes of (1) gaining better measures of interindividual differences in attitudes as compared to self-report measures (quality hope); (2) better predicting behavior, or predicting other behaviors, as compared to self-reports (incremental validity hope); (3) linking social-cognitive theories more adequately to empirical research (theory-link hope). We argue that the third hope should be the starting point for using these measures. Any attempt to improve these measures should include the search for a small-scale theory that adequately explains the basic effects found with such a measure. To date, small-scale theories for different measures are not equally well developed.

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