Fluorescent Silicon Nanorods-Based Ratiometric Sensors for Long-Term and Real-Time Measurements of Intracellular pH in Live Cells

2017 ◽  
Vol 89 (22) ◽  
pp. 12152-12159 ◽  
Author(s):  
Binbin Chu ◽  
Bin Song ◽  
Xiaoyuan Ji ◽  
Yuanyuan Su ◽  
Houyu Wang ◽  
...  
Keyword(s):  
Real Time ◽  
Intracellular Ph ◽  
Live Cells ◽  
Ratiometric Sensors

Fluorescent and Photostable Silicon Nanoparticles Sensors for Real-Time and Long-Term Intracellular pH Measurement in Live Cells

2016 ◽  
Vol 88 (18) ◽  
pp. 9235-9242 ◽  
Author(s):  
Binbin Chu ◽  
Houyu Wang ◽  
Bin Song ◽  
Fei Peng ◽  
Yuanyuan Su ◽  
...  
Keyword(s):  
Real Time ◽  
Intracellular Ph ◽  
Silicon Nanoparticles ◽  
Ph Measurement ◽  
Live Cells

Real-time monitoring of intracellular pH in live cells with fluorescent ionic liquid

2020 ◽  
Vol 1111 ◽  
pp. 132-138 ◽  
Author(s):  
Lifang Gao ◽  
Xin Lin ◽  
Anqi Zheng ◽  
E. Shuang ◽  
Jianhua Wang ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
Real Time ◽  
Intracellular Ph ◽  
Live Cells ◽  
Real Time Monitoring

scholarly journals Techno-Economic Planning and Operation of the Microgrid Considering Real-Time Pricing Demand Response Program

Energies ◽  
2021 ◽  
Vol 14 (15) ◽  
pp. 4597
Author(s):  
Zi-Xuan Yu ◽  
Meng-Shi Li ◽  
Yi-Peng Xu ◽  
Sheraz Aslam ◽  
Yuan-Kang Li
Keyword(s):  
Real Time ◽  
Demand Response ◽  
Positive Impact ◽  
Environmental Aspects ◽  
Component Size ◽  
Cost Of Energy ◽  
Four Seasons ◽  
Demand Response Program ◽  
Real Time Pricing

The optimal planning of grid-connected microgrids (MGs) has been extensively studied in recent years. While most of the previous studies have used fixed or time-of-use (TOU) prices for the optimal sizing of MGs, this work introduces real-time pricing (RTP) for implementing a demand response (DR) program according to the national grid prices of Iran. In addition to the long-term planning of MG, the day-ahead operation of MG is also analyzed to get a better understanding of the DR program for daily electricity dispatch. For this purpose, four different days corresponding to the four seasons are selected for further analysis. In addition, various impacts of the proposed DR program on the MG planning results, including sizing and best configuration, net present cost (NPC) and cost of energy (COE), and emission generation by the utility grid, are investigated. The optimization results show that the implementation of the DR program has a positive impact on the technical, economic, and environmental aspects of MG. The NPC and COE are reduced by about USD 3700 and USD 0.0025/kWh, respectively. The component size is also reduced, resulting in a reduction in the initial cost. Carbon emissions are also reduced by 185 kg/year.

scholarly journals Ligand-Mediated Assembly and Real-Time Cellular Dynamics of Estrogen Receptor α-Coactivator Complexes in Living Cells

2001 ◽  
Vol 21 (13) ◽  
pp. 4404-4412 ◽  
Author(s):  
David L. Stenoien ◽  
Anne C. Nye ◽  
Maureen G. Mancini ◽  
Kavita Patel ◽  
Martin Dutertre ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Real Time ◽  
Fluorescent Protein ◽  
Yellow Fluorescent Protein ◽  
Steroid Receptor ◽  
Estrogen Receptor Α ◽  
Living Cells ◽  
Live Cells ◽  
Creb Binding Protein ◽  
High Accumulation

ABSTRACT Studies with live cells demonstrate that agonist and antagonist rapidly (within minutes) modulate the subnuclear dynamics of estrogen receptor α (ER) and steroid receptor coactivator 1 (SRC-1). A functional cyan fluorescent protein (CFP)-taggedlac repressor-ER chimera (CFP-LacER) was used in live cells to discretely immobilize ER on stably integratedlac operator arrays to study recruitment of yellow fluorescent protein (YFP)-steroid receptor coactivators (YFP–SRC-1 and YFP-CREB binding protein [CBP]). In the absence of ligand, YFP–SRC-1 is found dispersed throughout the nucleoplasm, with a surprisingly high accumulation on the CFP-LacER arrays. Agonist addition results in the rapid (within minutes) recruitment of nucleoplasmic YFP–SRC-1, while antagonist additions diminish YFP–SRC-1–CFP-LacER associations. Less ligand-independent colocalization is observed with CFP-LacER and YFP-CBP, but agonist-induced recruitment occurs within minutes. The agonist-induced recruitment of coactivators requires helix 12 and critical residues in the ER–SRC-1 interaction surface, but not the F, AF-1, or DNA binding domains. Fluorescence recovery after photobleaching indicates that YFP–SRC-1, YFP-CBP, and CFP-LacER complexes undergo rapid (within seconds) molecular exchange even in the presence of an agonist. Taken together, these data suggest a dynamic view of receptor-coregulator interactions that is now amenable to real-time study in living cells.

Intensifiers on Tyneside

2010 ◽  
Vol 31 (3) ◽  
pp. 252-287 ◽  
Author(s):  
Katie Barnfield ◽  
Isabelle Buchstaller
Keyword(s):  
Real Time ◽  
Longitudinal Analysis ◽  
Developmental Trajectories ◽  
Time Data ◽  
Longitudinal Changes ◽  
Real Time Data ◽  
Quantitative Development ◽  
First Time

We report on longitudinal changes in the system of intensification in an innovative corpus that spans five decades of dialectal speech. Our analyses allow us — for the first time in a British context — to trace the quantitative development in the variable across four generations. Longitudinal analysis across real and apparent time determines the effect of extralinguistic and intralinguistic variables on intensification in Tyneside and tests to what extent real time data corroborates trends reported from previous apparent time analyses. Long-term competition within the variable manifests itself in distinctive developmental trajectories: expansion — both proportionally within the variable as well as across adjectival categories — tends to follow one of three types of patterns, exemplified, respectively, by really, so and dead. Variant retraction, however, follows only one schema. Importantly, numerical decline in the system does not necessarily go hand in hand with a reduction in breadth of application.

LONG TERM EFFECTS OF THYROID STIMULATING HORMONE AND INSULIN ON INTRACELLULAR pH IN FRTL-5 CELLS

1992 ◽  
Vol 133 (2) ◽  
pp. R9-R11
Author(s):  
A.M. Wood ◽  
S.P. Bidey ◽  
J. Soden ◽  
W.R. Robertson
Keyword(s):  
Intracellular Ph ◽  
Small Groups ◽  
Thyroid Stimulating Hormone ◽  
Ph Sensitive ◽  
Long Term Effects ◽  
Bicarbonate Ions ◽  
Chronic Effects ◽  
Petri Dishes ◽  
Presence And Absence

ABSTRACT We have studied the chronic effects of TSH (100μU/ml) and insulin (10μg/ml) on intracellular pH (pHi) in FRTL-5 cells using the pH sensitive probe 2′7-bis (2-carboxyethyl-5′-6′) carboxyfluorescein. FRTL-5 cells were cultured on Petri dishes either in the presence of 4H, ie. Coons F-12 containing cortisol (10nM), transferrin (0.5μg/ml), glycyl-histidyl lysine acetate (10ng/ml) and somatostatin (10μg/ml), or with 4H+insulin (5H), 4H+TSH, or 4H+TSH+insulin (6H). pHi was measured in small groups of cells by microspectrofluorimetry both in the presence and absence of bicarbonate ions after cells had been deprived of serum for at least a day. In

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