Nanoscale Metal–Organic Frameworks for Real-Time Intracellular pH Sensing in Live Cells

: Adenosine 5'-triphosphate (ATP) plays a significant role in biological processes and the ATP level is closely associated with many diseases. In order to detect ATP in live cells, tissues and body fluids with a high sensitivity and selectivity, researchers have developed various sensing strategies. Particularly, owing to distinct physicochemical properties of nanomaterials and high sensitivity of fluorescence, a great deal of efforts have been devoted to developing nanomaterials-based approaches for fluorescent ATP sensing in recent years. In this review, we focus on the current development of nanomaterial-based fluorescent ATP sensors and discuss the sensing mechanisms in detail. The advantages and disadvantages of ATP sensing using different kinds of nanomaterials, including carbon nanomaterials, metal nanoparticles, semiconductor quantum dots, metal-organic frameworks and up-conversion nanoparticles have been thoroughly compared and discussed. Finally, current challenges and future prospects in this field are given.

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A Wide Range of pH-Sensitive Fluorescent Probe based on Responsive Double Hydrophilic Block Copolymer for Real-Time Intracellular pH Sensing in Water Samples

IOP Conference Series Materials Science and Engineering ◽

10.1088/1757-899x/562/1/012053 ◽

2019 ◽

Vol 562 ◽

pp. 012053

Author(s):

Zijuan Zhou

Keyword(s):

Block Copolymer ◽

Real Time ◽

Fluorescent Probe ◽

Intracellular Ph ◽

Water Samples ◽

Ph Sensitive ◽

Ph Sensing ◽

Wide Range

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Anion directing self-assembly of 2D and 3D water-stable silver(i) cation metal organic frameworks and their applications in real-time discriminating cysteine and DNA detection

Journal of Materials Chemistry B ◽

10.1039/c8tb01032c ◽

2018 ◽

Vol 6 (28) ◽

pp. 4569-4574 ◽

Cited By ~ 10

Author(s):

Xin Rui Wang ◽

Jing Du ◽

Zhuo Huang ◽

Kun Liu ◽

Yuan Yuan Liu ◽

...

Keyword(s):

Real Time ◽

Self Assembly ◽

Dna Detection ◽

Metal Organic Frameworks ◽

Metal Organic ◽

2D And 3D

Two water-stable silver(i) cation metal organic frameworks (MOFs), namely 2D MOF {[Ag(L)2]BF4}n (1) and 3D MOF {[Ag3(L)3]·(H2O)·(CF3SO3)3}n (2) (L = 1-(4-aminobenzyl)-1,2,4-triazole), have been prepared.

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Rapid and sensitive analysis of progesterone by solid-phase extraction with amino-functionalized metal-organic frameworks coupled to direct analysis in real-time mass spectrometry

Analytical and Bioanalytical Chemistry ◽

10.1007/s00216-020-02535-6 ◽

2020 ◽

Vol 412 (12) ◽

pp. 2939-2947 ◽

Cited By ~ 3

Author(s):

Linnan Li ◽

Yilin Chen ◽

Yuangui Yang ◽

Ying Yang ◽

Li Yang ◽

...

Keyword(s):

Mass Spectrometry ◽

Solid Phase Extraction ◽

Real Time ◽

Solid Phase ◽

Metal Organic Frameworks ◽

Direct Analysis ◽

Phase Extraction ◽

Sensitive Analysis ◽

Metal Organic

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Inside Cover: Highly Stable Mixed-Lanthanide Metal-Organic Frameworks for Self-Referencing and Colorimetric Luminescent pH Sensing (ChemNanoMat 1/2017)

ChemNanoMat ◽

10.1002/cnma.201600355 ◽

2016 ◽

Vol 3 (1) ◽

pp. 2-2

Author(s):

Tifeng Xia ◽

Yuanjing Cui ◽

Yu Yang ◽

Guodong Qian

Keyword(s):

Metal Organic Frameworks ◽

Ph Sensing ◽

Metal Organic ◽

Lanthanide Metal

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Cu-based metal-organic frameworks-derived copper nanoclusters with tunable emission for ratiometric pH sensing

Sensors and Actuators B Chemical ◽

10.1016/j.snb.2021.131130 ◽

2021 ◽

pp. 131130

Author(s):

Xue Hu ◽

Xiaodan Zhang ◽

Haiyan Cao ◽

Yuming Huang

Keyword(s):

Metal Organic Frameworks ◽

Ph Sensing ◽

Copper Nanoclusters ◽

Metal Organic ◽

Ratiometric Ph Sensing ◽

Tunable Emission

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Fe-Hemin-Metal Organic Frameworks/Three-Dimensional Graphene Composites with Efficient Peroxidase-Like Bioactivity for Real-Time Electrochemical Detection of Extracellular Hydrogen Peroxide

Journal of The Electrochemical Society ◽

10.1149/1945-7111/ac3b04 ◽

2021 ◽

Author(s):

Shiying Zhou ◽

Human Sun ◽

Xianfeng Wang ◽

Peng Lu ◽

Danqun Huo ◽

...

Keyword(s):

Hydrogen Peroxide ◽

Real Time ◽

Metal Organic Frameworks ◽

Three Dimensional ◽

A549 Cells ◽

Dynamic Monitoring ◽

Serum Samples ◽

Time Dynamic ◽

Metal Organic ◽

Time Determination

Abstract Real-time detection of extracellular hydrogen peroxide is important for dynamic monitoring of diseases and cytopathological research. Here, a novel composite of Fe-hemin-metal organic frameworks/three-dimensional graphene (Fe-hemin MOFs/3DG) was prepared by embedding hemin into amino-contained Fe-MOFs, then decorated with 3DG. The obtained Fe-hemin MOFs/3DG possessed efficient peroxidase-like bioactivity and could serve as an effective catalyst for construction of H2O2 electrochemical sensor. The electrochemical results show that the proposed sensor based on Fe-hemin MOFs/3DG has excellent catalytic activity for H2O2. With a linear range was 10 - 12080 μM and LOD was 0.34 μM, this sensor showed favorable selectivity, repeatability and stability, which could be used to detect H2O2 in real serum samples. Most importantly, this sensor realized the real-time determination of H2O2 released by A549 cells and possessed good biocompatibility. The outstanding electrochemical performance mainly benefited from the unique porous structure of MOFs, which could effectively protect the active center of hemin, and the introduction of 3DG greatly improved the conductivity of Fe-hemin MOFs. Therefore, the Fe-hemin MOFs/3DG could be a promising probe for real-time dynamic monitoring of H2O2.

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