Soft and Hard Interactions between Polystyrene Nanoplastics and Human Serum Albumin Protein Corona

2019 ◽  
Vol 30 (4) ◽  
pp. 1067-1076 ◽  
Author(s):  
Shinji Kihara ◽  
Nadine J. van der Heijden ◽  
Chris K. Seal ◽  
Jitendra P. Mata ◽  
Andrew E. Whitten ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Protein Corona

scholarly journals A study on human serum albumin corona formed on photoluminescent carbon dots

2020 ◽  
Vol 44 (7-8) ◽  
pp. 447-452
Author(s):  
Peng Wang ◽  
Ming Yuan ◽  
Na Li ◽  
Feng Zhang
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Carbon Dots ◽  
Resonance Energy ◽  
Binding Constants ◽  
Protein Corona ◽  
Hybrid Nanomaterial ◽  
Corona Formation ◽  
Biomedical Field

Fluorescence nanostructures have been widely applied in the biomedical field as therapeutic agents and as novel tools for labeling, imaging, and sensing. However, the protein corona will dramatically influence the predesigned properties of nanostructures in serum. Therefore, it is important to understand the mechanism of protein corona formation on nanostructures. Photoluminescent carbon dots have been widely applied in the biomedical field since their discovery. Due to the large overlap between the absorption spectra of proteins and the fluorescence spectra of photoluminescent carbon dots, herein we investigate the mechanism of human serum albumin corona formed on photoluminescent carbon dots using fluorescence resonance energy transfer. By employing spectroscopic methods, the binding constants and the number of binding sites between human serum albumin and photoluminescent carbon dots have been determined, and the corresponding thermodynamics are also discussed as well for the interaction between photoluminescent carbon dots and human serum albumin. In addition, we successfully demonstrate the photoluminescent carbon dots in labeling bean sprouts. We believe that the current research cannot shed light on the mechanism of protein corona formation on nanostructures, but also could benefit the design of hybrid nanomaterial which will be applied to serum environments.

Human serum albumin stabilizes aqueous silver nanoparticle suspensions and inhibits particle uptake by cells

2018 ◽  
Vol 5 (4) ◽  
pp. 863-867 ◽  
Author(s):  
D. C. Kennedy ◽  
H. Qian ◽  
V. Gies ◽  
L. Yang
Keyword(s):  
Silver Nanoparticles ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Silver Nanoparticle ◽  
Protein Corona ◽  
Particle Uptake ◽  
Nanoparticle Suspensions ◽  
Commercial Polymers ◽  
Better Than

Human serum albumin forms a protein corona that stabilizes silver nanoparticles in water better than commercial polymers.

Molecularly Imprinted Nanogels Possessing Dansylamide Interaction Sites for Controlling Protein Corona In Situ by Cloaking Intrinsic Human Serum Albumin

Langmuir ◽  
2020 ◽  
Vol 36 (36) ◽  
pp. 10674-10682 ◽  
Author(s):  
Takahiro Morishita ◽  
Aoi Yoshida ◽  
Natsuki Hayakawa ◽  
Kentaro Kiguchi ◽  
Chehasan Cheubong ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Protein Corona ◽  
Molecularly Imprinted ◽  
Interaction Sites

scholarly journals Are Zinc oxide nanoparticles safe? A structural study on human serum albumin using in vitro and in silico methods

2018 ◽  
Author(s):  
Marziyeh Hassanian ◽  
Hassan Aryapour ◽  
Alireza Goudarzi ◽  
Masoud Bezi Javan
Keyword(s):  
Zinc Oxide ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
In Silico ◽  
Zinc Oxide Nanoparticles ◽  
Protein Corona ◽  
Oxide Nanoparticles ◽  
Zno Nps

AbstractWith due attention to adsorption of proteins on the nanoparticles surface and the formation of nanoparticle-protein corona, investigation of nanoparticles toxicity on the structure of proteins is important. Therefore, this work was done to evaluate toxicity of Zinc oxide nanoparticles (ZnO NPs) on the structure of human serum albumin (HSA) through in vitro and in silico studies. First, ZnO NPs were synthesized using hydrothermal method and their size and morphology were determined by SEM and TEM methods and then to study its toxicity on the HSA structure were used UV-Vis and fluorescence spectroscopy. Also, in order to investigate interaction mechanism of ZnO NP with HSA at the atomistic level was used molecular dynamics (md) simulation. The obtained images from SEM and TEM showed that ZnO NPs were nanosheet with size of less than 40 nm. The results of spectroscopic studies showed ZnO NPs lead to significant conformational changes in the protein’s absorption and emission spectra. Moreover, md results showed the minor structure changes in HSA due to interaction with ZnO NP during the 100 ns simulation and the formation of nanoparticle-protein corona complex that is mainly because of electrostatic interactions between charge groups of HSA and ZnO NP.

Protein corona formation of human serum albumin with carbon quantum dots from roast salmon

2020 ◽  
Vol 11 (3) ◽  
pp. 2358-2367 ◽  
Author(s):  
Yukun Song ◽  
Haitao Wang ◽  
Lijuan Zhang ◽  
Bin Lai ◽  
Kangjing Liu ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Protein Corona ◽  
Carbon Quantum Dots ◽  
Corona Formation

Carbon quantum dots (CQDs) extracted from roast salmon could interact with human serum albumin (HSA) to form protein coronas, changing their biological identity.

scholarly journals Effects of surface functionalization on the adsorption of human serum albumin onto nanoparticles – a fluorescence correlation spectroscopy study

2014 ◽  
Vol 5 ◽  
pp. 2036-2047 ◽  
Author(s):  
Pauline Maffre ◽  
Stefan Brandholt ◽  
Karin Nienhaus ◽  
Li Shang ◽  
Wolfgang J Parak ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Fluorescence Correlation Spectroscopy ◽  
Pt Nanoparticles ◽  
Protein Corona ◽  
Correlation Spectroscopy ◽  
Surface Charges ◽  
Fluorescence Correlation ◽  
Coulombic Interactions

By using fluorescence correlation spectroscopy (FCS), we have studied the adsorption of human serum albumin (HSA) onto Fe–Pt nanoparticles (NPs, 6 nm radius), CdSe/ZnS quantum dots (QDs, 5 nm radius) and Au and Ag nanoclusters (1–4 nm radius), which are enshrouded by various water-solubilizing surface layers exposing different chemical functional groups (carboxyl, amino and both), thereby endowing the NPs with different surface charges. We have also measured the effects of modified surface functionalizations on the protein via succinylation and amination. A step-wise increase in hydrodynamic radius with protein concentration was always observed, revealing formation of protein monolayers coating the NPs, independent of their surface charge. The differences in the thickness of the protein corona were rationalized in terms of the different orientations in which HSA adsorbs onto the NPs. The midpoints of the binding transition, which quantifies the affinity of HSA toward the NP, were observed to differ by almost four orders of magnitude. These variations can be understood in terms of specific Coulombic interactions between the proteins and the NP surfaces.

scholarly journals Role of the Protein Corona Derived from Human Plasma in Cellular Interactions between Nanoporous Human Serum Albumin Particles and Endothelial Cells

2017 ◽  
Vol 28 (8) ◽  
pp. 2062-2068 ◽  
Author(s):  
Mikhail V. Zyuzin ◽  
Yan Yan ◽  
Raimo Hartmann ◽  
Katelyn T. Gause ◽  
Moritz Nazarenus ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Human Plasma ◽  
Protein Corona ◽  
Cellular Interactions

II. Lokalisation der Placenta mit 113Inm- Human-Serum-Albumin

1969 ◽  
Vol 08 (01) ◽  
pp. 15-21 ◽  
Author(s):  
K. E. Scheer ◽  
J. Heep ◽  
W. Maier-Borst ◽  
W. J. Lorenz ◽  
H. Sinn ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Vena Cava ◽  
Placenta Praevia ◽  
Wird Eine

ZusammenfassungNach tierexperimentellen Voruntersuchungen wurde die Placentographie mit trägerfreiem 113Inm -HSA als klinische Methode eingeführt. Vor Amniocentesen und bei Verdacht auf Placenta praevia werden Placentographien geschrieben. Den Schwangeren wird eine Aktivität von 500 μCi in die Cubitalvene injiziert. Die der Aktivität entsprechende Indiummenge ist kleiner als 0,1 ng. Die fetale Strahlenbelastung liegt unter lOmrad. Bei Anwendung von 113Inm-HSA entfällt eine Blockade der mütterlichen und fetalen Schilddrüsen. Die genaue Abgrenzung einer Placenta praevia wird nicht durch eine Blasenaktivität beeinträchtigt.Es wurden bisher 19 Placentalokalisationen durchgeführt. In allen Fällen konnte der Placentasitz eindeutig festgestellt werden. Bedingt durch die lange Liegezeit beim Aufnehmen eines Szintigramms kam es in zwei Fällen zu einem Vena-Cava-Kompressions-Syndrom. Zur Verhinderung dieser klinischen Zwischenfälle werden inzwischen Placentographien mit der Anger-Kamera aufgenommen. Mit Hilfe des divergierenden Kollimators konnte der gesamte Abdominalbereich erfaßt werden. Die Aufnahmezeit konnte auf 7 — 10 Minuten verkürzt werden. Die intravenöse injizierte Aktivität betrug bei dieser Methode ebenfalls 500 μCi. Der diagnostische Aussagewert der Kamerabilder ist szintigraphischen Aufnahmen gleichwertig.

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