X-ray Visible and Uniform Alginate Microspheres Loaded with in Situ Synthesized BaSO4 Nanoparticles for in Vivo Transcatheter Arterial Embolization

2015 ◽  
Vol 16 (4) ◽  
pp. 1240-1246 ◽  
Author(s):  
Qin Wang ◽  
Kun Qian ◽  
Shanshan Liu ◽  
Yajiang Yang ◽  
Bin Liang ◽  
...  
Keyword(s):  
Transcatheter Arterial Embolization ◽  
Arterial Embolization ◽  
X Ray

scholarly journals Development of a sticker sealed microfluidic device for in situ analytical measurements using synchrotron radiation

2021 ◽  
Author(s):  
ITAMAR NECKEL ◽  
Lucas F. de Castro ◽  
Flavia Callefo ◽  
Verônica Teixeira ◽  
Angelo Gobbi ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Green Energy ◽  
X Rays ◽  
Electrical Measurements ◽  
X Ray ◽  
Electrochemical Nucleation ◽  
Synchrotron Light

Abstract Shedding synchrotron light on microfluidic systems, exploring several contrasts in situ operando at the nanoscale, like X-ray fluorescence, diffraction, luminescence, and absorption, has the potential to reveal new properties and functionalities of materials across diverse areas, such as green energy, photonics, and nanomedicine. In this work, we present the micro-fabrication and characterization of a multifunctional polyester/glass sealed microfluidic device well-suited to combine with analytical X-ray techniques. The device consists of smooth microchannels patterned on glass, where three gold electrodes are deposited into the channels to serve in situ electrochemistry analysis or standard electrical measurements. It has been efficiently sealed through an ultraviolet-sensitive sticker-like layer based on a polyester film, and The burst pressure determined by pumping water through the microchannel(up to 0.22 MPa). Overall, the device has demonstrated exquisite chemical resistance to organic solvents, and its efficiency in the presence of biological samples (proteins) is remarkable. The device potentialities, and its high transparency to X-rays, have been demonstrated by taking advantage of the X-ray nanoprobe Carnaúba/Sirius/LNLS, by obtaining 2D X-ray nanofluorescence maps on the microchannel filled with water and after an electrochemical nucleation reaction. To wrap up, the microfluidic device characterized here has the potential to be employed in standard laboratory experiments as well as in situ and in vivo analytical experiments using a wide electromagnetic window, from infrared to X-rays, which could serve experiments in many branches of science.

scholarly journals Embolism resistance in petioles and leaflets of palms

2019 ◽  
Vol 124 (7) ◽  
pp. 1173-1183 ◽  
Author(s):  
Thaise Emilio ◽  
Laurent J Lamarque ◽  
José M Torres-Ruiz ◽  
Andrew King ◽  
Guillaume Charrier ◽  
...  
Keyword(s):  
Hydraulic Conductivity ◽  
Xylem Embolism ◽  
Phylogenetic Relatedness ◽  
X Ray ◽  
Xylem Pressure ◽  
Palm Species ◽  
Embolism Resistance ◽  
Increasing Temperature

Abstract Background and aims Hydraulic studies are currently biased towards conifers and dicotyledonous angiosperms; responses of arborescent monocots to increasing temperature and drought remain poorly known. This study aims to assess xylem resistance to drought-induced embolism in palms. Methods We quantified embolism resistance via P50 (xylem pressure inducing 50 % embolism or loss of hydraulic conductivity) in petioles and leaflets of six palm species differing in habitat and phylogenetic relatedness using three techniques: in vivo X-ray-based microcomputed tomography, the in situ flow centrifuge technique and the optical vulnerability method. Key results Our results show that P50 of petioles varies greatly in the palm family, from −2.2 ± 0.4 MPa in Dypsis baronii to −5.8 ± 0.3 MPa in Rhapis excelsa (mean ± s.e.). No difference or weak differences were found between petioles and leaf blades within species. Surprisingly, where differences occurred, leaflets were less vulnerable to embolism than petioles. Embolism resistance was not correlated with conduit size (r = 0.37, P = 0.11). Conclusions This study represents the first estimate of drought-induced xylem embolism in palms across biomes and provides the first step towards understanding hydraulic adaptations in long-lived arborescent monocots. It showed an almost 3-fold range of embolism resistance between palm species, as large as that reported in all angiosperms. We found little evidence for hydraulic segmentation between leaflets and petioles in palms, suggesting that when it happens, hydraulic segregation may lack a clear relationship with organ cost or replaceability.

Temperature sensitive p(N-isopropylacrylamide-co-acrylic acid) modified gold nanoparticles for trans-arterial embolization and angiography

2017 ◽  
Vol 5 (5) ◽  
pp. 907-916 ◽  
Author(s):  
Yiming Liu ◽  
Xiaole Peng ◽  
Kun Qian ◽  
Yingying Ma ◽  
Jiangshan Wan ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Acrylic Acid ◽  
Blood Vessel ◽  
Arterial Embolization ◽  
Temperature Sensitive ◽  
X Ray ◽  
X Ray Imaging

Temperature-sensitive in situ hydrogels of GNP@PNA nanomedicine achieve good blood-vessel embolization and X-ray imaging on rabbits.

scholarly journals Development of a sticker sealed microfluidic device for in situ analytical measurements using synchrotron radiation

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Itamar T. Neckel ◽  
Lucas F. de Castro ◽  
Flavia Callefo ◽  
Verônica C. Teixeira ◽  
Angelo L. Gobbi ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Green Energy ◽  
X Rays ◽  
Electrical Measurements ◽  
X Ray ◽  
Electrochemical Nucleation ◽  
Synchrotron Light

AbstractShedding synchrotron light on microfluidic systems, exploring several contrasts in situ/operando at the nanoscale, like X-ray fluorescence, diffraction, luminescence, and absorption, has the potential to reveal new properties and functionalities of materials across diverse areas, such as green energy, photonics, and nanomedicine. In this work, we present the micro-fabrication and characterization of a multifunctional polyester/glass sealed microfluidic device well-suited to combine with analytical X-ray techniques. The device consists of smooth microchannels patterned on glass, where three gold electrodes are deposited into the channels to serve in situ electrochemistry analysis or standard electrical measurements. It has been efficiently sealed through an ultraviolet-sensitive sticker-like layer based on a polyester film, and The burst pressure determined by pumping water through the microchannel(up to 0.22 MPa). Overall, the device has demonstrated exquisite chemical resistance to organic solvents, and its efficiency in the presence of biological samples (proteins) is remarkable. The device potentialities, and its high transparency to X-rays, have been demonstrated by taking advantage of the X-ray nanoprobe Carnaúba/Sirius/LNLS, by obtaining 2D X-ray nanofluorescence maps on the microchannel filled with water and after an electrochemical nucleation reaction. To wrap up, the microfluidic device characterized here has the potential to be employed in standard laboratory experiments as well as in in situ and in vivo analytical experiments using a wide electromagnetic window, from infrared to X-rays, which could serve experiments in many branches of science.

scholarly journals MODERN OPPORTUNITIES OF USING TRANSCATHETER ARTERIAL EMBOLIZATION IN BLEEDING FROM THE ABDOMINAL ORGANS OF NON-TRAUMATIC GENESIS (LITERATURE REVIEW)

2021 ◽  
Vol 21 (4) ◽  
pp. 54-62
Author(s):  
A. N. Sekeev ◽  
V. G. Verbitsky ◽  
G. I. Sinenchenko ◽  
S. А. Alentyev ◽  
А. Е. Demko ◽  
...  
Keyword(s):  
Acute Pancreatitis ◽  
Gastrointestinal Tract ◽  
Literature Review ◽  
Transcatheter Arterial Embolization ◽  
Arterial Embolization ◽  
Ulcer Bleeding ◽  
Present Stage ◽  
X Ray ◽  
Abdominal Organs ◽  
Surgical Methods

At the present stage treatment of gastrointestinal bleeding, x-ray surgical methods play an important role. This review examines the features of the use of transcatheter arterial embolization in the most relevant nosological forms: ulcer bleeding, bleeding in chronic and acute pancreatitis, and in decaying tumors of the gastrointestinal tract.

Development of Microcapsules Containing Oil-Based Contrast Medium

2011 ◽  
Vol 687 ◽  
pp. 548-551
Author(s):  
Masumi Uehara ◽  
Syotaro Hamakawa ◽  
Yasuyuki Maki ◽  
Toshiaki Dobashi
Keyword(s):  
Hepatocellular Carcinoma ◽  
Contrast Medium ◽  
Transcatheter Arterial Embolization ◽  
Interfacial Polymerization ◽  
Arterial Embolization ◽  
Embolization Therapy ◽  
X Ray ◽  
The Core ◽  
Average Size ◽  
X Ray Absorption

For efficient transcatheter arterial embolization therapy for hepatocellular carcinoma, we have developed microcapsules containing oil-based contrast medium, as obstructing materials. The microcapsules were prepared by means of the interfacial polymerization to make a wall membrane consisting of polyureaurethane with Lipiodol in the core. The average size of the particles was 80 μm, and the size distribution was fairly small. The extent of X-ray absorption of the microcapsules was controlled by the ratio of the amounts of triisocyanate monomer and Lipiodol in the oil phase on the interfacial polymerization.

scholarly journals Serial femtosecond X-ray diffraction of in vivo crystals in intact yeast cells

2014 ◽  
Vol 70 (a1) ◽  
pp. C570-C570
Author(s):  
Daniel Passon ◽  
Arjen Jakobi ◽  
Francesco Stellato ◽  
Mengning Liang ◽  
Kevin Knoops ◽  
...  
Keyword(s):  
Yeast Cells ◽  
Protein Crystal ◽  
Crystal Formation ◽  
Proof Of Concept ◽  
X Ray Diffraction ◽  
X Ray ◽  
In Situ Crystallization ◽  
Diffraction Patterns

Peroxisomes are membrane-enclosed organelles in eukaryotic cells with important roles in lipid metabolism and the scavenging of reactive oxygen species. Peroxisomes are capable of carrying an unusually high load of proteins, which under appropriate nutrient conditions results in the in situ crystallization of peroxisomal proteins in several yeast species and vertebrate hepatocytes [1,2]. In the methylotrophic yeast H.polymorpha, the predominant peroxisomal protein alcohol/methanol oxidase (AO) oligomerizes into octameric assemblies with a molecular mass of 600 kDa that spontaneously form 200-500 nm crystallites within peroxisomes [1]. We exposed H.polymorpha cell suspensions containing peroxisome-confined AO crystallites to femtosecond X-ray pulses at the Coherent X-ray Imaging (CXI) experimental endstation at the Linac Coherent Light Source. Peak detection routines mining the resulting scattering profiles identified >5000 Bragg-sampled diffraction patterns, providing the proof of concept that background scattering from the cells does not deteriorate the signal-to-noise ratio to an extent precluding observation of diffraction from individual AO crystallites. Summation patterns assembled from the individual frames match low-resolution powder diffraction patterns from concentrated suspensions of purified peroxisomes collected at the P14 beamline at the PETRAIII synchrotron, confirming that the observed diffraction mainly results from Bragg scattering of peroxisomal crystallites. To the best of our knowledge our data are the first to report room temperature X-ray diffraction from functional protein crystals in their native cellular environment. Currently the maximum resolution achieved in the diffraction patterns is limited to 20-15 Å. Future work will need to address improved sample preparation protocols in order to assess whether diffraction to a resolution sufficient to permit structure solution can be obtained. Protein crystal formation in vivo has been observed under physiological or pathological conditions in a number of other systems [3]. We hope that our results will help to establish serial femtosecond X-ray diffraction (SFX) as a method for structural characterization of cellular structures with crystalline content and provide a proof of concept for using in situ crystallization of proteins as a means to generate nanocrystalline samples for SFX.

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