Cellular Responses to Exposure to Outdoor Air from the Chinese Spring Festival at the Air–Liquid Interface

2019 ◽  
Vol 53 (15) ◽  
pp. 9128-9138 ◽  
Author(s):  
Jie Ding ◽  
Jincheng Guo ◽  
Liming Wang ◽  
Yandong Chen ◽  
Bin Hu ◽  
...  
Keyword(s):  
Chinese Spring ◽  
Cellular Responses ◽  
Liquid Interface ◽  
Outdoor Air ◽  
Spring Festival ◽  
Air Liquid Interface

scholarly journals Silica nanoparticles are less toxic to human lung cells when deposited at the air–liquid interface compared to conventional submerged exposure

2014 ◽  
Vol 5 ◽  
pp. 1590-1602 ◽  
Author(s):  
Alicja Panas ◽  
Andreas Comouth ◽  
Harald Saathoff ◽  
Thomas Leisner ◽  
Marco Al-Rawi ◽  
...  
Keyword(s):  
Cell Culture ◽  
Silica Nanoparticles ◽  
Amorphous Silica ◽  
Particle Deposition ◽  
Biological Effects ◽  
Cellular Responses ◽  
Liquid Interface ◽  
Lung Cells ◽  
Air Liquid Interface ◽  
Submerged Conditions

Background: Investigations on adverse biological effects of nanoparticles (NPs) in the lung by in vitro studies are usually performed under submerged conditions where NPs are suspended in cell culture media. However, the behaviour of nanoparticles such as agglomeration and sedimentation in such complex suspensions is difficult to control and hence the deposited cellular dose often remains unknown. Moreover, the cellular responses to NPs under submerged culture conditions might differ from those observed at physiological settings at the air–liquid interface. Results: In order to avoid problems because of an altered behaviour of the nanoparticles in cell culture medium and to mimic a more realistic situation relevant for inhalation, human A549 lung epithelial cells were exposed to aerosols at the air–liquid interphase (ALI) by using the ALI deposition apparatus (ALIDA). The application of an electrostatic field allowed for particle deposition efficiencies that were higher by a factor of more than 20 compared to the unmodified VITROCELL deposition system. We studied two different amorphous silica nanoparticles (particles produced by flame synthesis and particles produced in suspension by the Stöber method). Aerosols with well-defined particle sizes and concentrations were generated by using a commercial electrospray generator or an atomizer. Only the electrospray method allowed for the generation of an aerosol containing monodisperse NPs. However, the deposited mass and surface dose of the particles was too low to induce cellular responses. Therefore, we generated the aerosol with an atomizer which supplied agglomerates and thus allowed a particle deposition with a three orders of magnitude higher mass and of surface doses on lung cells that induced significant biological effects. The deposited dose was estimated and independently validated by measurements using either transmission electron microscopy or, in case of labelled NPs, by fluorescence analyses. Surprisingly, cells exposed at the ALI were less sensitive to silica NPs as evidenced by reduced cytotoxicity and inflammatory responses. Conclusion: Amorphous silica NPs induced qualitatively similar cellular responses under submerged conditions and at the ALI. However, submerged exposure to NPs triggers stronger effects at much lower cellular doses. Hence, more studies are warranted to decipher whether cells at the ALI are in general less vulnerable to NPs or specific NPs show different activities dependent on the exposure method.

Rapid, efficient and dose-controlled delivery of liquid aerosol to pulmonary cells cultured at the air-liquid interface

Pneumologie ◽  
2011 ◽  
Vol 65 (12) ◽  
Author(s):  
M Selmansberger ◽  
AG Lenz ◽  
M Schmidmeir ◽  
O Eickelberg ◽  
T Stoeger ◽  
...  
Keyword(s):  
Liquid Interface ◽  
Controlled Delivery ◽  
Pulmonary Cells ◽  
Air Liquid Interface ◽  
Cells Cultured

Efficient bioactive delivery of aerosolized drugs to pulmonary cells at the air-liquid interface: Validation of the ALICE-CLOUD technology

Pneumologie ◽  
2014 ◽  
Vol 68 (02) ◽  
Author(s):  
AG Lenz ◽  
T Stoeger ◽  
D Cei ◽  
M Schmidmeir ◽  
N Pfister ◽  
...  
Keyword(s):  
Liquid Interface ◽  
Cloud Technology ◽  
Pulmonary Cells ◽  
Air Liquid Interface

Zwischen Luft und Wasser – Air-Liquid-Interface-Kulturen als In-vitro-Modell des Respirationstrakts

2020 ◽  
Author(s):  
S. Runft ◽  
L. Burigk ◽  
A. Lehmbecker ◽  
K. Schöne ◽  
D. Waschke ◽  
...  
Keyword(s):  
Liquid Interface ◽  
Air Liquid Interface

scholarly journals Drag on a spherical particle at the air–liquid interface: Interplay between compressibility, Marangoni flow, and surface viscosities

2021 ◽  
Vol 33 (6) ◽  
pp. 062103
Author(s):  
Meisam Pourali ◽  
Martin Kröger ◽  
Jan Vermant ◽  
Patrick D. Anderson ◽  
Nick O. Jaensson
Keyword(s):  
Spherical Particle ◽  
Liquid Interface ◽  
Marangoni Flow ◽  
Air Liquid Interface

Nonclassical Surface Nucleation of 6CB at the Air–Liquid Interface of a 6CB Oil-in-Water Nanoemulsion

Langmuir ◽  
2021 ◽  
Author(s):  
Jin Tae Park ◽  
Govind Paneru ◽  
Masao Iwamatsu ◽  
Bruce M. Law ◽  
Hyuk Kyu Pak
Keyword(s):  
Liquid Interface ◽  
Surface Nucleation ◽  
Oil In Water ◽  
Air Liquid Interface

scholarly journals Effect of Alpha-1 Antitrypsin on CFTR Levels in Primary Human Airway Epithelial Cells Grown at the Air-Liquid-Interface

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2639
Author(s):  
Frauke Stanke ◽  
Sabina Janciauskiene ◽  
Stephanie Tamm ◽  
Sabine Wrenger ◽  
Ellen Luise Raddatz ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Human Lung ◽  
Liquid Interface ◽  
Lung Epithelial Cells ◽  
Western Blots ◽  
Mesenchymal Transition ◽  
Protein Levels ◽  
Lung Epithelial ◽  
Cftr Protein ◽  
Air Liquid Interface

The cystic fibrosis transmembrane conductance regulator (CFTR) gene is influenced by the fundamental cellular processes like epithelial differentiation/polarization, regeneration and epithelial–mesenchymal transition. Defects in CFTR protein levels and/or function lead to decreased airway surface liquid layer facilitating microbial colonization and inflammation. The SERPINA1 gene, encoding alpha1-antitrypsin (AAT) protein, is one of the genes implicated in CF, however it remains unknown whether AAT has any influence on CFTR levels. In this study we assessed CFTR protein levels in primary human lung epithelial cells grown at the air-liquid-interface (ALI) alone or pre-incubated with AAT by Western blots and immunohistochemistry. Histological analysis of ALI inserts revealed CFTR- and AAT-positive cells but no AAT-CFTR co-localization. When 0.5 mg/mL of AAT was added to apical or basolateral compartments of pro-inflammatory activated ALI cultures, CFTR levels increased relative to activated ALIs. This finding suggests that AAT is CFTR-modulating protein, albeit its effects may depend on the concentration and the route of administration. Human lung epithelial ALI cultures provide a useful tool for studies in detail how AAT or other pharmaceuticals affect the levels and activity of CFTR.

scholarly journals A robust SARS-CoV-2 replication model in primary human epithelial cells at the air liquid interface to assess antiviral agents

2021 ◽  
pp. 105122
Author(s):  
Thuc Nguyen Dan Do ◽  
Kim Donckers ◽  
Laura Vangeel ◽  
Arnab K. Chatterjee ◽  
Philippe A. Gallay ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Antiviral Agents ◽  
Liquid Interface ◽  
Human Epithelial Cells ◽  
Replication Model ◽  
Air Liquid Interface
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