Preparation of Casein Biopeptide-Loaded Niosomes by High Shear Homogenization and Their Characterization

Caseinate-stabilized emulsions of black cumin (Nigella sativa) and tamanu (Calophyllum inophyllum) oils were studied in terms of preparation, characterization, and antibacterial properties. The oils were described while using their basic characteristics, including fatty acid composition and scavenging activity. The oil-in-water (o/w) emulsions containing the studied oils were formulated, and the influence of protein stabilizer (sodium caseinate (CAS), 1–12 wt%), oil contents (5–30 wt%), and emulsification methods (high-shear homogenization vs sonication) on the emulsion properties were investigated. It was observed that, under both preparation methods, emulsions of small, initial droplet sizes were predominantly formed with CAS content that was higher than 7.5 wt%. Sonication was a more efficient emulsification procedure and was afforded emulsions with smaller droplet size throughout the entire used concentration ranges of oils and CAS when compared to high-shear homogenization. At native pH of ~ 6.5, all of the emulsions exhibited negative zeta potential that originated from the presence of caseinate. The antibacterial activities of both oils and their emulsions were investigated with respect to the growth suppression of common spoilage bacteria while using the disk diffusion method. The oils and selected emulsions were proven to act against gram positive strains, mainly against Staphylococcus aureus (S. aureus) and Bacillus cereus (B. cereus); regrettably, the gram negative species were fully resistant against their action.

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Effect of Solid Lipid and Liquid Oil Ratios on Properties of Nanostructured Lipid Carriers for Oral Curcumin Delivery

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.1060.62 ◽

2014 ◽

Vol 1060 ◽

pp. 62-65 ◽

Cited By ~ 9

Author(s):

Yaowaporn Sangsen ◽

Punsupang Laochai ◽

Pravara Chotsathidchai ◽

Ruedeekorn Wiwattanapatapee

Keyword(s):

Particle Size ◽

Zeta Potential ◽

Sustained Release ◽

Physicochemical Properties ◽

Nanostructured Lipid Carriers ◽

Loading Capacity ◽

High Shear ◽

Solid Lipid ◽

High Shear Homogenization ◽

Xrd Techniques

In this study, three nanostructured lipid carriers (NLC) formulations comprised of varying ratios of lipid (Compritol® 888 ATO) and oil (Labrafac® CC) including 4:1, 3:2, and 2.5:2.5, were developed by high shear homogenization technique. The effect of different ratios on the physicochemical properties and release profiles of the formulations were investigated. Increasing the amount of liquid oil increased the particle size and zeta potential whereas decreased size distribution of the blank and curcumin loaded NLC. However, the entrapment efficacy and loading capacity of the NLC for curcumin were not increased following such ratios. The different ratios were not influence on the sequence of sustained release of curcumin from the NLC over 60 h. Moreover, the amorphous curcumin and crystalline behavior of the optimized NLC were characterized by DSC and XRD techniques. Thus, the effect of the proportions of solid lipid and liquid oil in the formulations should be considered for development of suitable NLC system for oral curcumin delivery.

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Optimization of β-carotene loaded solid lipid nanoparticles preparation using a high shear homogenization technique

Journal of Nanoparticle Research ◽

10.1007/s11051-008-9402-3 ◽

2008 ◽

Vol 11 (3) ◽

pp. 601-614 ◽

Cited By ~ 55

Author(s):

Michael D. Triplett ◽

James F. Rathman

Keyword(s):

Solid Lipid Nanoparticles ◽

Lipid Nanoparticles ◽

High Shear ◽

Solid Lipid ◽

Homogenization Technique ◽

High Shear Homogenization ◽

Β Carotene

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Olanzapine Loaded Nanostructured Lipid Carriers via High Shear Homogenization and Ultrasonication

Scientia Pharmaceutica ◽

10.3390/scipharm89020025 ◽

2021 ◽

Vol 89 (2) ◽

pp. 25

Author(s):

Adejumoke Lara Ajiboye ◽

Uttom Nandi ◽

Martin Galli ◽

Vivek Trivedi

Keyword(s):

Particle Size ◽

Drug Release ◽

Drug Loading ◽

Particle Diameter ◽

Phosphate Buffer Solution ◽

Nanostructured Lipid Carriers ◽

High Shear ◽

Buffer Solution ◽

High Shear Homogenization

The aim of this study was to understand the effect of high shear homogenization (HSH) and ultrasonication (US) on the physicochemical properties of blank and olanzapine loaded nanostructured lipid carriers (NLCs) along with their drug loading potential and drug release profiles from formulated particles. NLCs were prepared with different ratios of Compritol and Miglyol as the solid and liquid lipids, respectively, under changing HSH and US times between 0 to 15 minutes. The surfactants (Poloxamer 188 (P188) and tween 80) and the drug content was kept constant in all formulations. The prepared NLCs were evaluated for particle size, polydispersity index, zeta potential, drug crystallinity and chemical interactions between lipids and OLZ. The in-vitro drug release was performed using dialysis tube method in phosphate buffer solution (PBS) at pH 7.4. The formulated NLCs were negatively charged, spherically shaped and monodisperse, with particle sizes ranging from 112 to 191 nm. There was a significant influence of US time on the preparation of NLCs in comparison to HSH, where a significant reduction in the mean particle diameter was seen after 5 min of sonication. An increase of Miglyol content in NLCs led to an increase in particle size. In general, application of US led to decrease in particle size after HSH but an increase in particle diameter of low Miglyol containing preparation was also observed with longer sonication time. OLZ was successfully encapsulated in the NLCs and a total release of 89% was achieved in 24 hours in PBS at pH 7.4.

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