Analytical Strategies for LC–MS-Based Untargeted and Targeted Metabolomics Approaches Reveal the Entomological Origins of Honey

2022 ◽  
Author(s):  
Xinran Wang ◽  
Yi Li ◽  
Lanzhen Chen ◽  
Jinhui Zhou
Keyword(s):  
Targeted Metabolomics ◽  
Analytical Strategies

Strategies for large-scale targeted metabolomics quantification by liquid chromatography-mass spectrometry

The Analyst ◽  
2016 ◽  
Vol 141 (23) ◽  
pp. 6362-6373 ◽  
Author(s):  
Juntuo Zhou ◽  
Yuxin Yin
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Large Scale ◽  
Liquid Chromatography Mass Spectrometry ◽  
Targeted Metabolomics ◽  
Chromatography Mass Spectrometry ◽  
Great Progress ◽  
Analytical Strategies ◽  
Metabolomics Analysis

Advances in liquid chromatography-mass spectrometry (LC-MS) instruments and analytical strategies have brought about great progress in targeted metabolomics analysis.

scholarly journals Analytical strategies for LC–MS-based targeted metabolomics

2008 ◽  
Vol 871 (2) ◽  
pp. 236-242 ◽  
Author(s):  
Wenyun Lu ◽  
Bryson D. Bennett ◽  
Joshua D. Rabinowitz
Keyword(s):  
Targeted Metabolomics ◽  
Analytical Strategies

Effects of Psychotherapy on Brain Activation Patterns in Anxiety Disorders

2016 ◽  
Vol 224 (2) ◽  
pp. 62-70 ◽  
Author(s):  
Thomas Straube
Keyword(s):  
Anxiety Disorders ◽  
Neuronal Plasticity ◽  
Functional Neuroimaging ◽  
State Of The Art ◽  
Methodological Issues ◽  
Future Directions ◽  
Activation Patterns ◽  
New Learning ◽  
Analytical Strategies ◽  
Sample Characteristics

Abstract. Psychotherapy is an effective treatment for most mental disorders, including anxiety disorders. Successful psychotherapy implies new learning experiences and therefore neural alterations. With the increasing availability of functional neuroimaging methods, it has become possible to investigate psychotherapeutically induced neuronal plasticity across the whole brain in controlled studies. However, the detectable effects strongly depend on neuroscientific methods, experimental paradigms, analytical strategies, and sample characteristics. This article summarizes the state of the art, discusses current theoretical and methodological issues, and suggests future directions of the research on the neurobiology of psychotherapy in anxiety disorders.

Diversity of diterpenoids and polyphenols in Salvia glutinosa L. analyzed using targeted metabolomics based on LC-qTOF-MS and 1H-NMR

2017 ◽  
Author(s):  
S Ślusarczyk ◽  
J JaŘpińska ◽  
Ł Pecio ◽  
A Stochmal ◽  
E Gille ◽  
...  
Keyword(s):  
1H Nmr ◽  
Targeted Metabolomics

Mechanisms of ageing metabolic decline revealed by targeted metabolomics and energy metabolism in NAD+ depleted skeletal muscle

2016 ◽  
Author(s):  
Rachel Fletcher ◽  
Lucy Oldacre-Bartley ◽  
Craig Doig ◽  
Charles Brenner ◽  
Gareth Lavery
Keyword(s):  
Skeletal Muscle ◽  
Energy Metabolism ◽  
Targeted Metabolomics

Comprehensive Analysis of Transcriptomics and Metabolomics between the Heads and Tails of Angelica Sinensis: Genes Related to Phenylpropanoid Biosynthesis Pathway

2020 ◽  
Vol 23 ◽  
Author(s):  
Jie Yang ◽  
Chi Zhang ◽  
Wei-Hong Li ◽  
Tian-Er Zhang ◽  
Guang-Zhong Fan ◽  
...  
Keyword(s):  
Ferulic Acid ◽  
Metabolic Regulation ◽  
Comprehensive Analysis ◽  
Angelica Sinensis ◽  
Rna Seq ◽  
Targeted Metabolomics ◽  
Kegg Pathways ◽  
Qrt Pcr ◽  
Phenylpropanoid Biosynthesis ◽  
Combined Strategy

Background:: In Traditional Chinese Medicine (TCM), the heads and tails of Angelica sinensis (Oliv.) Diels (AS) is used in treating different diseases due to their different pharmaceutical efficacies. The underline mechanisms, however, have not been fully explored. Objective:: Novel mechanisms responsible for the discrepant activities between AS heads and tails were explored by a combined strategy of transcriptomes and metabolomics. Method:: Six pairs of the heads and tails of AS roots were collected in Min County, China. Total RNA and metabolites, which were used for RNA-seq and untargeted metabolomics analysis, were respectively isolated from each AS sample (0.1 g) by Trizol and methanol reagent. Subsequently, differentially expressed genes (DEGs) and discrepant pharmaceutical metabolites were identified for comparing AS heads and tails. Key DEGs and metabolites were quantified by qRT-PCR and targeted metabolomics experiment. Results:: Comprehensive analysis of transcriptomes and metabolomics results suggested that five KEGG pathways with significant differences included 57 DEGs. Especially, fourteen DEGs and six key metabolites were relation to the metabolic regulation of Phenylpropanoid biosynthesis (PB) pathway. Results of qRT-PCR and targeted metabolomics indicated that higher levels of expression of crucial genes in PB pathway, such as PAL, CAD, COMT and peroxidase in the tail of AS were positively correlated with levels of ferulic acid-related metabolites. The average content of ferulic acid in tails (569.58162.39 nmol/g) was higher than those in the heads (168.73  67.30 nmol/g) (P˂0.01); Caffeic acid in tails (3.82  0.88 nmol/g) vs heads (1.37  0.41 nmol/g) (P˂0.01), and Cinnamic acid in tails (0.24  0.09 nmol/g) vs heads (0.14  0.02 nmol/g) (P˂0.05). Conclusion:: Our work demonstrated that overexpressed genes and accumulated metabolites derived from PB pathway might be responsible for the discrepant pharmaceutical efficacies between AS heads and tails.

scholarly journals P2.11-10 Discovery of Potential Biomarkers That Discriminate Early Stage NSCLC from Controls by Non-Targeted Metabolomics Profiling

2019 ◽  
Vol 14 (10) ◽  
pp. S795-S796
Author(s):  
J. Kim ◽  
R. Balshaw ◽  
C. Trevena ◽  
S. Banerji ◽  
L. Murphy ◽  
...  
Keyword(s):  
Early Stage ◽  
Targeted Metabolomics ◽  
Early Stage Nsclc ◽  
Potential Biomarkers
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